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The map label for this gene is lysC [H]

Identifier: 197117652

GI number: 197117652

Start: 1460726

End: 1461940

Strand: Direct

Name: lysC [H]

Synonym: Gbem_1264

Alternate gene names: 197117652

Gene position: 1460726-1461940 (Clockwise)

Preceding gene: 197117651

Following gene: 197117653

Centisome position: 31.65

GC content: 58.19

Gene sequence:

>1215_bases
ATGGCTTTGGTGGTCCAAAAGTACGGCGGCACCTCGATGGGTTCCGTCGAACGGATTCGTAACGTCGCTAAGAGGGTGGC
GAAAACCTACGATGCCGGCAACGACATGGTGGTGGTAGTCTCTGCCATGTCCGGCGAAACTAACAAGCTGGTGGCCCTTG
CCAACGAAGTCTGCGAATTCCCGGACAACCGCGAATACGACGTGCTGGTTGCAGCAGGCGAGCAGGTTTCCATCGCGTTG
CTGGCCATGTGCCTTAAATCCATGGGGTACAAAGCGAAATCCTACTTGGGCTTCCAGGTCCCCATTCAGACCGATAGCGC
CTACGCAAAGGCACGCATCGAGAAGATCGACGACGCCAAGATGCGCACCGATTTGAAGGAAGGGACCATCCTCATCGTCG
CGGGCTTCCAGGGTGTCGACCAGTCCGGCAGCGTCACCACGCTTGGGCGCGGCGGCTCGGACACCTCGGCGGTCGCTCTC
GCGGCGGCTCTGAAAGCGGACGTTTGCGAAATTTTCACCGACGTTGACGGGGTCTACACCACCGATCCCAACATCTGCAA
GGACGCGAAGAAGATCGAGCGCATCTCCTACGAGGAGATGCTGGAGTTGGCCAGCCTGGGCGCCAAGGTGCTCCAGATCC
GCTCCGTCGAATTCGCAAGCAAGTACAACGTCGACGTCCATGTCCGCTCAAGCTTTAACGAAAATCTCGGAACCATGGTT
ACCAAGGAGGATAAAGATATGGAAGCAGTACTCGTCTCGGGTATCGCCTATGCCAAGGATGAAGTGAAAATAGCCGTGAT
GCAGGTTCCGGACAAGCCGGGGATCGCCGCCCAGATCCTGTCGCCGCTCTCCGATGCCAATATTTCCGTGGACATGATCG
TTCAGAACGTGAGCGAGGCCGGTTCCACCGACTTCACCTTCACCGTGCCCCAGGCCGAATTCAAGAAGGCGCTGGCCATC
ACCCAGGAGACCGCCCAGGCGATCAACGCCAAGGAAGTCCTCTCCGACGAGAACGTGAGCAAGGTCTCCATCGTCGGCCT
CGGCATGAGGAGCCACGCAGGGGTCGCGACAACCATGTTCAAGGCGCTTGCCGCAGAAGGGATCAACATCCAGATGATCT
CCACCTCGGAGATCAAGATCTCCGTCGTCGTCGACGCCAAATACACCGAACTCGCCGTAAGGGTGCTGCACGACGTCTTC
GGCCTGTCGAAATAA

Upstream 100 bases:

>100_bases
AATGTTTTGACCGTGAACTCTATTCTTGCTAATAAAGGTACCTCCTAACCACCGGGGGATGCTTTCCGGGGATTAACAGT
TGAGTATAAGGAGGACTGAC

Downstream 100 bases:

>100_bases
CACCCGTTCAACGTTAGACGTTCGAGGTTTCGGAACCTTCTGTGTGCAGGGGACTGGCTCCGCAAGGTGCCCGTCCCCCT
GCAGCCGGAGGCCTTGGGAC

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MALVVQKYGGTSMGSVERIRNVAKRVAKTYDAGNDMVVVVSAMSGETNKLVALANEVCEFPDNREYDVLVAAGEQVSIAL
LAMCLKSMGYKAKSYLGFQVPIQTDSAYAKARIEKIDDAKMRTDLKEGTILIVAGFQGVDQSGSVTTLGRGGSDTSAVAL
AAALKADVCEIFTDVDGVYTTDPNICKDAKKIERISYEEMLELASLGAKVLQIRSVEFASKYNVDVHVRSSFNENLGTMV
TKEDKDMEAVLVSGIAYAKDEVKIAVMQVPDKPGIAAQILSPLSDANISVDMIVQNVSEAGSTDFTFTVPQAEFKKALAI
TQETAQAINAKEVLSDENVSKVSIVGLGMRSHAGVATTMFKALAAEGINIQMISTSEIKISVVVDAKYTELAVRVLHDVF
GLSK

Sequences:

>Translated_404_residues
MALVVQKYGGTSMGSVERIRNVAKRVAKTYDAGNDMVVVVSAMSGETNKLVALANEVCEFPDNREYDVLVAAGEQVSIAL
LAMCLKSMGYKAKSYLGFQVPIQTDSAYAKARIEKIDDAKMRTDLKEGTILIVAGFQGVDQSGSVTTLGRGGSDTSAVAL
AAALKADVCEIFTDVDGVYTTDPNICKDAKKIERISYEEMLELASLGAKVLQIRSVEFASKYNVDVHVRSSFNENLGTMV
TKEDKDMEAVLVSGIAYAKDEVKIAVMQVPDKPGIAAQILSPLSDANISVDMIVQNVSEAGSTDFTFTVPQAEFKKALAI
TQETAQAINAKEVLSDENVSKVSIVGLGMRSHAGVATTMFKALAAEGINIQMISTSEIKISVVVDAKYTELAVRVLHDVF
GLSK
>Mature_403_residues
ALVVQKYGGTSMGSVERIRNVAKRVAKTYDAGNDMVVVVSAMSGETNKLVALANEVCEFPDNREYDVLVAAGEQVSIALL
AMCLKSMGYKAKSYLGFQVPIQTDSAYAKARIEKIDDAKMRTDLKEGTILIVAGFQGVDQSGSVTTLGRGGSDTSAVALA
AALKADVCEIFTDVDGVYTTDPNICKDAKKIERISYEEMLELASLGAKVLQIRSVEFASKYNVDVHVRSSFNENLGTMVT
KEDKDMEAVLVSGIAYAKDEVKIAVMQVPDKPGIAAQILSPLSDANISVDMIVQNVSEAGSTDFTFTVPQAEFKKALAIT
QETAQAINAKEVLSDENVSKVSIVGLGMRSHAGVATTMFKALAAEGINIQMISTSEIKISVVVDAKYTELAVRVLHDVFG
LSK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=349, Percent_Identity=32.9512893982808, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1790455, Length=453, Percent_Identity=28.6975717439294, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790376, Length=239, Percent_Identity=28.8702928870293, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320893, Length=494, Percent_Identity=23.8866396761134, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43240; Mature: 43109

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVQKYGGTSMGSVERIRNVAKRVAKTYDAGNDMVVVVSAMSGETNKLVALANEVCEF
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
PDNREYDVLVAAGEQVSIALLAMCLKSMGYKAKSYLGFQVPIQTDSAYAKARIEKIDDAK
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHHHCCCHH
MRTDLKEGTILIVAGFQGVDQSGSVTTLGRGGSDTSAVALAAALKADVCEIFTDVDGVYT
HHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
TDPNICKDAKKIERISYEEMLELASLGAKVLQIRSVEFASKYNVDVHVRSSFNENLGTMV
CCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHEEEEEHHHHCCEEEEEECCCCCCCCCEE
TKEDKDMEAVLVSGIAYAKDEVKIAVMQVPDKPGIAAQILSPLSDANISVDMIVQNVSEA
ECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHC
GSTDFTFTVPQAEFKKALAITQETAQAINAKEVLSDENVSKVSIVGLGMRSHAGVATTMF
CCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
KALAAEGINIQMISTSEIKISVVVDAKYTELAVRVLHDVFGLSK
HHHHHCCCEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ALVVQKYGGTSMGSVERIRNVAKRVAKTYDAGNDMVVVVSAMSGETNKLVALANEVCEF
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCC
PDNREYDVLVAAGEQVSIALLAMCLKSMGYKAKSYLGFQVPIQTDSAYAKARIEKIDDAK
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHHHHCCCHH
MRTDLKEGTILIVAGFQGVDQSGSVTTLGRGGSDTSAVALAAALKADVCEIFTDVDGVYT
HHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
TDPNICKDAKKIERISYEEMLELASLGAKVLQIRSVEFASKYNVDVHVRSSFNENLGTMV
CCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHEEEEEHHHHCCEEEEEECCCCCCCCCEE
TKEDKDMEAVLVSGIAYAKDEVKIAVMQVPDKPGIAAQILSPLSDANISVDMIVQNVSEA
ECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHC
GSTDFTFTVPQAEFKKALAITQETAQAINAKEVLSDENVSKVSIVGLGMRSHAGVATTMF
CCCCEEEECCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCCCCCHHHHHH
KALAAEGINIQMISTSEIKISVVVDAKYTELAVRVLHDVFGLSK
HHHHHCCCEEEEEECCCEEEEEEEECHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]