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The map label for this gene is ispA [C]

Identifier: 197117645

GI number: 197117645

Start: 1453419

End: 1454306

Strand: Direct

Name: ispA [C]

Synonym: Gbem_1257

Alternate gene names: 197117645

Gene position: 1453419-1454306 (Clockwise)

Preceding gene: 197117644

Following gene: 197117646

Centisome position: 31.49

GC content: 64.75

Gene sequence:

>888_bases
ATGGATCTGAAGCAATATTTGAAGCAGAAGTGTGAGTTGGTCGACCAGGCGCTGGAGCGCTTTCTTCCCCATGGGAAGGA
ACTACCGGCGTCCCTGCACGGTTCCATGCGCTACTCGGTGTTTGCCGGAGGCAAAAGGGTGCGCCCGGTGCTCTTGCTGG
CCGCCTGCGAGGCGGTGGGGGGGGAGCTTAACTCCGCCCTGCCGGCTGCCTGCGCCATGGAGATGATCCACACCTACTCC
CTCATCCACGACGACCTCCCCGCCATGGACAACGACGACTTCCGCCGGGGGAACCCCACCAACCACAAGGTGTACGGCGA
GGCGACCGCCATCTTAGCCGGCGACGCGCTCTTAACCGAGGCCTTCATCCTCCTCTCCTGCAACGGCGAAGGGATGGAGC
CTGGGGCGCGCCTTCGGGTGATCCACGAGATAGCGCACGCTTCCGGCTCGCGCGGGATGGTTGGCGGGCAGATGGTCGAC
ATGGAGAGCGAGGGAAAGCACGACATCGACATGGCTACCCTCTCCTACATCCATACCCACAAGACCGGTGCCCTGATCCG
GGCTTCGGTGCGCGCCGGCGCCATCCTCGGCGGGGCGAACGAGGAGACCTTCGACGCCCTCACCCGTTACGCCGACGCCA
TCGGCCTCGCCTTCCAGATCGCGGACGACGTTCTCGACGTGGAGGGGACCACCGAGGAGTTGGGAAAGGACGCCGGGAGC
GACCAGGCCCGCGGCAAGGCGACCTACCCGGCGCTCGTCGGGCTGGAGGCGTCCAAACGGCGCGCCGAAGAACTGGTGCA
GATCGCCTTGAACGCGCTGGAGCCGTTCGACGAGCGGGCAGAACCTTTGCGGGCCATAGCTTCGTACATAGTGAAAAGGA
AATCGTAG

Upstream 100 bases:

>100_bases
AAGCAGCGCGACGGCAGCTTCGTCACCAAGCCATTCGAGGAAGAGGAATAGAGAGCCGCGGGACGCGGCCAACGTAACTT
ATCGAAAAGTGAGACATGAT

Downstream 100 bases:

>100_bases
ATGCCGTCCATCCTGGAAGGGATTAAAGAACCGGGGCAGTTGAAGGCGCTCTCGCTGAGCGAGCTGGAAACCCTTGCGGC
CGAGCTCCGCGAGCGGATCA

Product: trans-isoprenyl diphosphate synthase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MDLKQYLKQKCELVDQALERFLPHGKELPASLHGSMRYSVFAGGKRVRPVLLLAACEAVGGELNSALPAACAMEMIHTYS
LIHDDLPAMDNDDFRRGNPTNHKVYGEATAILAGDALLTEAFILLSCNGEGMEPGARLRVIHEIAHASGSRGMVGGQMVD
MESEGKHDIDMATLSYIHTHKTGALIRASVRAGAILGGANEETFDALTRYADAIGLAFQIADDVLDVEGTTEELGKDAGS
DQARGKATYPALVGLEASKRRAEELVQIALNALEPFDERAEPLRAIASYIVKRKS

Sequences:

>Translated_295_residues
MDLKQYLKQKCELVDQALERFLPHGKELPASLHGSMRYSVFAGGKRVRPVLLLAACEAVGGELNSALPAACAMEMIHTYS
LIHDDLPAMDNDDFRRGNPTNHKVYGEATAILAGDALLTEAFILLSCNGEGMEPGARLRVIHEIAHASGSRGMVGGQMVD
MESEGKHDIDMATLSYIHTHKTGALIRASVRAGAILGGANEETFDALTRYADAIGLAFQIADDVLDVEGTTEELGKDAGS
DQARGKATYPALVGLEASKRRAEELVQIALNALEPFDERAEPLRAIASYIVKRKS
>Mature_295_residues
MDLKQYLKQKCELVDQALERFLPHGKELPASLHGSMRYSVFAGGKRVRPVLLLAACEAVGGELNSALPAACAMEMIHTYS
LIHDDLPAMDNDDFRRGNPTNHKVYGEATAILAGDALLTEAFILLSCNGEGMEPGARLRVIHEIAHASGSRGMVGGQMVD
MESEGKHDIDMATLSYIHTHKTGALIRASVRAGAILGGANEETFDALTRYADAIGLAFQIADDVLDVEGTTEELGKDAGS
DQARGKATYPALVGLEASKRRAEELVQIALNALEPFDERAEPLRAIASYIVKRKS

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=226, Percent_Identity=30.5309734513274, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1786623, Length=299, Percent_Identity=46.4882943143813, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1789578, Length=233, Percent_Identity=31.7596566523605, Blast_Score=118, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17505681, Length=187, Percent_Identity=32.620320855615, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24651612, Length=230, Percent_Identity=30.4347826086957, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 31773; Mature: 31773

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKQYLKQKCELVDQALERFLPHGKELPASLHGSMRYSVFAGGKRVRPVLLLAACEAVG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHC
GELNSALPAACAMEMIHTYSLIHDDLPAMDNDDFRRGNPTNHKVYGEATAILAGDALLTE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHC
AFILLSCNGEGMEPGARLRVIHEIAHASGSRGMVGGQMVDMESEGKHDIDMATLSYIHTH
EEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHC
KTGALIRASVRAGAILGGANEETFDALTRYADAIGLAFQIADDVLDVEGTTEELGKDAGS
CCCHHEEHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
DQARGKATYPALVGLEASKRRAEELVQIALNALEPFDERAEPLRAIASYIVKRKS
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLKQYLKQKCELVDQALERFLPHGKELPASLHGSMRYSVFAGGKRVRPVLLLAACEAVG
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEEEEEEECCCCHHHHHHHHHHHHHC
GELNSALPAACAMEMIHTYSLIHDDLPAMDNDDFRRGNPTNHKVYGEATAILAGDALLTE
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHC
AFILLSCNGEGMEPGARLRVIHEIAHASGSRGMVGGQMVDMESEGKHDIDMATLSYIHTH
EEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHC
KTGALIRASVRAGAILGGANEETFDALTRYADAIGLAFQIADDVLDVEGTTEELGKDAGS
CCCHHEEHHHHHCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
DQARGKATYPALVGLEASKRRAEELVQIALNALEPFDERAEPLRAIASYIVKRKS
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]