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The map label for this gene is btuB [H]

Identifier: 197117566

GI number: 197117566

Start: 1370672

End: 1372537

Strand: Reverse

Name: btuB [H]

Synonym: Gbem_1178

Alternate gene names: 197117566

Gene position: 1372537-1370672 (Counterclockwise)

Preceding gene: 197117576

Following gene: 197117565

Centisome position: 29.74

GC content: 64.52

Gene sequence:

>1866_bases
TTGAGACGTAACTTCGTAACCAAATTACTGTTTCCATGCGCGCTGTTGTCTGCCGTCCTGGCCGCGGCCCCCGCGCTGGG
ATCGGGGCAGGAGGAACTGATCGGTTCCTTCGGCCTCTCCGACCCCCCTCCCGAACTTGCGCGCTTTCCCCGCCCTGCCT
CCCGTATCGCCGAAAATATCACCGTCGTCACGGCGCAAGATATCGCCCGCATCAACGCCCACACTGTCGCCGAAGTGCTG
AATACCCTCCCGGGGGTCCAGATGGACCAGCTGCAGACGCCGGGATCGATGAACTTCTTCGGGGTCCTGGGCGCATCGAG
CCGGCACGTCCTGGTGCAGATCGACGGCGTGGCCCAGAACCTCATCGGTTCGGAAAACATAGCCCAGGTGGGGCTCATCC
CGGTGCAGATGATCGAGCGCATCGAGGTGGTGAAGGGGGCAGCTTCGGCGGCCTGGGGCTCGGCGCTGGGCGGGGTGATC
AACATCGTCACCAAGAGCCCTGCCGCGGACCGCGCCGCCGGCATGGTTTCGGGGTCCACCGGCAAGAGCGCCACCCGTGA
CCTGCGCGCCGAGGCGACCGGCAGCGGGGAGCGCTTCGGCTACTACCTGACCGCCGGCAAGCTTAGCTCCGACGGTCTCT
TGGCCGGCAACGACACCGACATGAACCACGGCTTCGGCAAATTGAGCTACGACTTTCCCAACGGGGGGGATCTTTCCCTG
AGCCTCGACGCGCGGCACAGCATGGCGGGCACCGAGGAGTCGCGGCGGTACGATTTTCACAACACCGGCGCCGCGACCCA
TGCCACGGGGAACCTGGTGCTCAACTACCCGCTGGCGGAGCGGCTTGCCCTGGAGGCGAACGCGCACACGGGGAGGCGGG
AGGTGCAGAACCGGCGGGCTGTCCTCACCACGGGGGTCCCCTTCGCCGACTTCAAAGGCCAGGAGACGTTCCGCGGAGCC
GACGCCGCGCTCAGTTGGGGAGACGCAAGCCGTGCCCTGAAAGCGGGGGTCTCCTTGGAGGAGACCGAGGTCGAAATGCA
CGAGCCGGTGAGACGGTATCCGCAGGCGAACTACGACCTTGCCCTCAGGCGGGAGTCGGCCTACCTGAACGGGACCTACA
CGATGGGAAGGCTCTCCATCCTGCCGGGTGTGCGCTTGGACCGCCTAAGTCTTATGGAAGATCCGGTCAGTTTCACTTTG
GGCGCCACGCTGCGTCTGACGGATAACACGCTGCTGCGCGGCTACGCGGCGTACGGCTATAGCATGCCGATCATCTCTAA
CCTCGGTATCCAGAACGGGCAGGTGCAGCGGGACCTGCAGCACGTACGGACGGTGCAGGCAGGAGTTGAGAGCGCGGATC
TCCCCTTCGTCTGGGTGAAAACAACCTTCTTCTACGCAGAGGTCTGGAACATCCAGGACTTCGACCAGCCGACGCCGCAG
CTCAGCCAGCAGGTGAAGCAGGGGGCCGAGCTGGAGCTGAAGAGCTCCCCCTGGTGCGGGTTCGCCCTAAACGGCTCCTA
CAACTTCACCGACGCCCGCGACCAAAAGACCGGGAAGGTGCTTTCCGTACTGGATGCGGGGCCGCGGCAGGCGCTGAAGG
TCGGGGTGAGATACGACAGCGAACCGGCAGGGCTTTCGGCTACGCTTCTGGGGGACTGGGCGAAGCTGCACAACCCGGGG
CGCTTCGCCAAGGACAAAGGGGAGTTCTGGGACCTCCACCTGACCCAGAAGCTCTCTCCTTCTTCGGACAACTCCCCGGA
GCTGTTCCTGTCCTGGCACAACATCTTCGACAGCAAACGGTACTTCTACGACTTCCGGGCCAACGCACCGCGCTGGTTAG
AGGCAGGGGCGAGGTGGTTTTTCTGA

Upstream 100 bases:

>100_bases
TCTTTGACACAATAATGCCAAACTGATACCAATATCACTTGGCGAATTTGTTCTCAGAAATTTCCCTGCTGTCAATCACT
CTTTGCTACAGGCTGCTCGC

Downstream 100 bases:

>100_bases
TGCGCGCCCTGGCCCTATTCCTACTGCTGGCGTTTTGCCTCCCCCCCCTCCCCTTAGCGCAGGGAGCCGAGCTGCTCATC
CTGGAGTCGAGCCGCAGCGC

Product: TonB-dependent receptor plug

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MRRNFVTKLLFPCALLSAVLAAAPALGSGQEELIGSFGLSDPPPELARFPRPASRIAENITVVTAQDIARINAHTVAEVL
NTLPGVQMDQLQTPGSMNFFGVLGASSRHVLVQIDGVAQNLIGSENIAQVGLIPVQMIERIEVVKGAASAAWGSALGGVI
NIVTKSPAADRAAGMVSGSTGKSATRDLRAEATGSGERFGYYLTAGKLSSDGLLAGNDTDMNHGFGKLSYDFPNGGDLSL
SLDARHSMAGTEESRRYDFHNTGAATHATGNLVLNYPLAERLALEANAHTGRREVQNRRAVLTTGVPFADFKGQETFRGA
DAALSWGDASRALKAGVSLEETEVEMHEPVRRYPQANYDLALRRESAYLNGTYTMGRLSILPGVRLDRLSLMEDPVSFTL
GATLRLTDNTLLRGYAAYGYSMPIISNLGIQNGQVQRDLQHVRTVQAGVESADLPFVWVKTTFFYAEVWNIQDFDQPTPQ
LSQQVKQGAELELKSSPWCGFALNGSYNFTDARDQKTGKVLSVLDAGPRQALKVGVRYDSEPAGLSATLLGDWAKLHNPG
RFAKDKGEFWDLHLTQKLSPSSDNSPELFLSWHNIFDSKRYFYDFRANAPRWLEAGARWFF

Sequences:

>Translated_621_residues
MRRNFVTKLLFPCALLSAVLAAAPALGSGQEELIGSFGLSDPPPELARFPRPASRIAENITVVTAQDIARINAHTVAEVL
NTLPGVQMDQLQTPGSMNFFGVLGASSRHVLVQIDGVAQNLIGSENIAQVGLIPVQMIERIEVVKGAASAAWGSALGGVI
NIVTKSPAADRAAGMVSGSTGKSATRDLRAEATGSGERFGYYLTAGKLSSDGLLAGNDTDMNHGFGKLSYDFPNGGDLSL
SLDARHSMAGTEESRRYDFHNTGAATHATGNLVLNYPLAERLALEANAHTGRREVQNRRAVLTTGVPFADFKGQETFRGA
DAALSWGDASRALKAGVSLEETEVEMHEPVRRYPQANYDLALRRESAYLNGTYTMGRLSILPGVRLDRLSLMEDPVSFTL
GATLRLTDNTLLRGYAAYGYSMPIISNLGIQNGQVQRDLQHVRTVQAGVESADLPFVWVKTTFFYAEVWNIQDFDQPTPQ
LSQQVKQGAELELKSSPWCGFALNGSYNFTDARDQKTGKVLSVLDAGPRQALKVGVRYDSEPAGLSATLLGDWAKLHNPG
RFAKDKGEFWDLHLTQKLSPSSDNSPELFLSWHNIFDSKRYFYDFRANAPRWLEAGARWFF
>Mature_621_residues
MRRNFVTKLLFPCALLSAVLAAAPALGSGQEELIGSFGLSDPPPELARFPRPASRIAENITVVTAQDIARINAHTVAEVL
NTLPGVQMDQLQTPGSMNFFGVLGASSRHVLVQIDGVAQNLIGSENIAQVGLIPVQMIERIEVVKGAASAAWGSALGGVI
NIVTKSPAADRAAGMVSGSTGKSATRDLRAEATGSGERFGYYLTAGKLSSDGLLAGNDTDMNHGFGKLSYDFPNGGDLSL
SLDARHSMAGTEESRRYDFHNTGAATHATGNLVLNYPLAERLALEANAHTGRREVQNRRAVLTTGVPFADFKGQETFRGA
DAALSWGDASRALKAGVSLEETEVEMHEPVRRYPQANYDLALRRESAYLNGTYTMGRLSILPGVRLDRLSLMEDPVSFTL
GATLRLTDNTLLRGYAAYGYSMPIISNLGIQNGQVQRDLQHVRTVQAGVESADLPFVWVKTTFFYAEVWNIQDFDQPTPQ
LSQQVKQGAELELKSSPWCGFALNGSYNFTDARDQKTGKVLSVLDAGPRQALKVGVRYDSEPAGLSATLLGDWAKLHNPG
RFAKDKGEFWDLHLTQKLSPSSDNSPELFLSWHNIFDSKRYFYDFRANAPRWLEAGARWFF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=135, Percent_Identity=40, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1788478, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67580; Mature: 67580

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNFVTKLLFPCALLSAVLAAAPALGSGQEELIGSFGLSDPPPELARFPRPASRIAENI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHCCCCHHHHHCCE
TVVTAQDIARINAHTVAEVLNTLPGVQMDQLQTPGSMNFFGVLGASSRHVLVQIDGVAQN
EEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEECCCCCEEEEEECCHHHH
LIGSENIAQVGLIPVQMIERIEVVKGAASAAWGSALGGVINIVTKSPAADRAAGMVSGST
HCCCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCHHHHCEECCCC
GKSATRDLRAEATGSGERFGYYLTAGKLSSDGLLAGNDTDMNHGFGKLSYDFPNGGDLSL
CCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCEEEECCCCCCCEEE
SLDARHSMAGTEESRRYDFHNTGAATHATGNLVLNYPLAERLALEANAHTGRREVQNRRA
EECCCCCCCCCCHHHCCCCCCCCCCEECCCCEEEECCHHHHHEEECCCCCCHHHHHCCCE
VLTTGVPFADFKGQETFRGADAALSWGDASRALKAGVSLEETEVEMHEPVRRYPQANYDL
EEEECCCCCCCCCCCHHCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCE
ALRRESAYLNGTYTMGRLSILPGVRLDRLSLMEDPVSFTLGATLRLTDNTLLRGYAAYGY
EEEECCCEECCEEEECEEEECCCCCCHHHHHHCCCCEEEECEEEEECCCHHHHHHHHCCC
SMPIISNLGIQNGQVQRDLQHVRTVQAGVESADLPFVWVKTTFFYAEVWNIQDFDQPTPQ
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCHH
LSQQVKQGAELELKSSPWCGFALNGSYNFTDARDQKTGKVLSVLDAGPRQALKVGVRYDS
HHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHEEECEECC
EPAGLSATLLGDWAKLHNPGRFAKDKGEFWDLHLTQKLSPSSDNSPELFLSWHNIFDSKR
CCCCCEEHEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEHHHCCCCE
YFYDFRANAPRWLEAGARWFF
EEEEECCCCCHHHHCCCCCCC
>Mature Secondary Structure
MRRNFVTKLLFPCALLSAVLAAAPALGSGQEELIGSFGLSDPPPELARFPRPASRIAENI
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCHHHHHCCCCHHHHHCCE
TVVTAQDIARINAHTVAEVLNTLPGVQMDQLQTPGSMNFFGVLGASSRHVLVQIDGVAQN
EEEEHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCEEEEECCCCCEEEEEECCHHHH
LIGSENIAQVGLIPVQMIERIEVVKGAASAAWGSALGGVINIVTKSPAADRAAGMVSGST
HCCCCCCHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCHHHHCEECCCC
GKSATRDLRAEATGSGERFGYYLTAGKLSSDGLLAGNDTDMNHGFGKLSYDFPNGGDLSL
CCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEECCCCCCCCCCCEEEECCCCCCCEEE
SLDARHSMAGTEESRRYDFHNTGAATHATGNLVLNYPLAERLALEANAHTGRREVQNRRA
EECCCCCCCCCCHHHCCCCCCCCCCEECCCCEEEECCHHHHHEEECCCCCCHHHHHCCCE
VLTTGVPFADFKGQETFRGADAALSWGDASRALKAGVSLEETEVEMHEPVRRYPQANYDL
EEEECCCCCCCCCCCHHCCCCCEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCE
ALRRESAYLNGTYTMGRLSILPGVRLDRLSLMEDPVSFTLGATLRLTDNTLLRGYAAYGY
EEEECCCEECCEEEECEEEECCCCCCHHHHHHCCCCEEEECEEEEECCCHHHHHHHHCCC
SMPIISNLGIQNGQVQRDLQHVRTVQAGVESADLPFVWVKTTFFYAEVWNIQDFDQPTPQ
CCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCHH
LSQQVKQGAELELKSSPWCGFALNGSYNFTDARDQKTGKVLSVLDAGPRQALKVGVRYDS
HHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHEEECEECC
EPAGLSATLLGDWAKLHNPGRFAKDKGEFWDLHLTQKLSPSSDNSPELFLSWHNIFDSKR
CCCCCEEHEEHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEHHHCCCCE
YFYDFRANAPRWLEAGARWFF
EEEEECCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA