Click here to switch to the map view.

The map label for this gene is yugF [H]

Identifier: 197117413

GI number: 197117413

Start: 1191941

End: 1192795

Strand: Reverse

Name: yugF [H]

Synonym: Gbem_1023

Alternate gene names: 197117413

Gene position: 1192795-1191941 (Counterclockwise)

Preceding gene: 197117419

Following gene: 197117412

Centisome position: 25.85

GC content: 62.81

Gene sequence:

>855_bases
ATGAACTCGCCGCTCGGGGCATGCCCCGTCTCCCCCAGTGAAACCCTCCGCTACCGCAGCTACGGCACCGGTCCCCGCAA
GATCGTCCTGGTTCACGGGCTTGCAGCCCGTTCCGAAACCTGGAAGGACCTGGTTCCGCTGTTCCCTGCCGACAGGTACA
CGGTCTACCTCCTGGATCTTTTAGGATCCGGGGAATCCGCCAAGCCACGGGAAGCGGACTACTCCATCAGGGGACACAGC
CGGAGGCTTCTTTGCTTCATCGATCGCGCGGGACTCCGGGGGGCGACGCTCGTGGGTCACTCTCTCGGTGGTGCGGTGGT
ACTGGTCGCAGCCGTCGAGGCCATGATCAAGGGGGATGCCGACGCCATCTCGGCCCTCGTGATAATGGGCGGTCCGGGGT
ACCTCCAACGGCTGCCGCTCATGGCCGAGATCTTCGAGAACCGTCTCGCCGCCGCACTTTTCATCGCACTGTACGCGCCG
GATATCTGGATCAAGGTGGGTTTGAAGATGGCCTACTACGACCAACGCCTCATCGACCGTGAGCACATCGCGCGCTACGC
CCCCTGCTATCGCAACCGGGACGCCAAGCGCGCCCTGGTCGAAACCTGCCGCTCGCTGGTTCCGGTGGACCAGGAAGAGA
TAACGGCCCGGTACGGGGACCTCGCGCTCCCGGTGTTGCTCCTTTGGGGGCGTCACGACCAGATCGTACCCCTGTCCCAG
GGATCCAGGCTGGAAGCCGCCATACCCGGGTCGAAGCTGCAGGTGATCGAGGAATGTGGTCATAACCCTCAGGAAGAAAA
GCCGCAAGTCACATTCAGCATCATCGAGAAGTTTCTGTTGCAAACCGCCGGCTAG

Upstream 100 bases:

>100_bases
TTCTCGAAAAGCCCGGTGAACTCTCATCGGGCTTTTTTATGCCTTAATTCTTTCACTTTAGACTGCCGCCTTCGGAAAGA
TTCTGCTACACTTGCCTGCC

Downstream 100 bases:

>100_bases
CCCACTGTCTGAGCGGAAGTAGAGTTAGCTGCGGCATTCTATGTCAGGGTTGATCTTGGGCTTGGCAGTGATATGTTTTT
CCAGTCCGAGCCCACATTAC

Product: hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNSPLGACPVSPSETLRYRSYGTGPRKIVLVHGLAARSETWKDLVPLFPADRYTVYLLDLLGSGESAKPREADYSIRGHS
RRLLCFIDRAGLRGATLVGHSLGGAVVLVAAVEAMIKGDADAISALVIMGGPGYLQRLPLMAEIFENRLAAALFIALYAP
DIWIKVGLKMAYYDQRLIDREHIARYAPCYRNRDAKRALVETCRSLVPVDQEEITARYGDLALPVLLLWGRHDQIVPLSQ
GSRLEAAIPGSKLQVIEECGHNPQEEKPQVTFSIIEKFLLQTAG

Sequences:

>Translated_284_residues
MNSPLGACPVSPSETLRYRSYGTGPRKIVLVHGLAARSETWKDLVPLFPADRYTVYLLDLLGSGESAKPREADYSIRGHS
RRLLCFIDRAGLRGATLVGHSLGGAVVLVAAVEAMIKGDADAISALVIMGGPGYLQRLPLMAEIFENRLAAALFIALYAP
DIWIKVGLKMAYYDQRLIDREHIARYAPCYRNRDAKRALVETCRSLVPVDQEEITARYGDLALPVLLLWGRHDQIVPLSQ
GSRLEAAIPGSKLQVIEECGHNPQEEKPQVTFSIIEKFLLQTAG
>Mature_284_residues
MNSPLGACPVSPSETLRYRSYGTGPRKIVLVHGLAARSETWKDLVPLFPADRYTVYLLDLLGSGESAKPREADYSIRGHS
RRLLCFIDRAGLRGATLVGHSLGGAVVLVAAVEAMIKGDADAISALVIMGGPGYLQRLPLMAEIFENRLAAALFIALYAP
DIWIKVGLKMAYYDQRLIDREHIARYAPCYRNRDAKRALVETCRSLVPVDQEEITARYGDLALPVLLLWGRHDQIVPLSQ
GSRLEAAIPGSKLQVIEECGHNPQEEKPQVTFSIIEKFLLQTAG

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=276, Percent_Identity=27.536231884058, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31280; Mature: 31280

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPLGACPVSPSETLRYRSYGTGPRKIVLVHGLAARSETWKDLVPLFPADRYTVYLLDL
CCCCCCCCCCCHHHHEEECCCCCCCCEEEEEECCHHCCHHHHHHHCCCCCCCCEEEEEEE
LGSGESAKPREADYSIRGHSRRLLCFIDRAGLRGATLVGHSLGGAVVLVAAVEAMIKGDA
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHCCCH
DAISALVIMGGPGYLQRLPLMAEIFENRLAAALFIALYAPDIWIKVGLKMAYYDQRLIDR
HHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCH
EHIARYAPCYRNRDAKRALVETCRSLVPVDQEEITARYGDLALPVLLLWGRHDQIVPLSQ
HHHHHHCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCEEECCC
GSRLEAAIPGSKLQVIEECGHNPQEEKPQVTFSIIEKFLLQTAG
CCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSPLGACPVSPSETLRYRSYGTGPRKIVLVHGLAARSETWKDLVPLFPADRYTVYLLDL
CCCCCCCCCCCHHHHEEECCCCCCCCEEEEEECCHHCCHHHHHHHCCCCCCCCEEEEEEE
LGSGESAKPREADYSIRGHSRRLLCFIDRAGLRGATLVGHSLGGAVVLVAAVEAMIKGDA
CCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHHCCCH
DAISALVIMGGPGYLQRLPLMAEIFENRLAAALFIALYAPDIWIKVGLKMAYYDQRLIDR
HHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCH
EHIARYAPCYRNRDAKRALVETCRSLVPVDQEEITARYGDLALPVLLLWGRHDQIVPLSQ
HHHHHHCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCEEECCC
GSRLEAAIPGSKLQVIEECGHNPQEEKPQVTFSIIEKFLLQTAG
CCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]