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The map label for this gene is pcm-2 [H]

Identifier: 197117384

GI number: 197117384

Start: 1150716

End: 1151375

Strand: Direct

Name: pcm-2 [H]

Synonym: Gbem_0994

Alternate gene names: 197117384

Gene position: 1150716-1151375 (Clockwise)

Preceding gene: 197117382

Following gene: 197117387

Centisome position: 24.93

GC content: 66.97

Gene sequence:

>660_bases
ATGACTGTCGACCCGAAAAGAGCGATGGAGGCCAGGAAGGAGCGGATGTTGAGCGAGCACCTTGCCGCGCGGGGGATTCG
CGACGCGGCCGTAATGCAGGCGATGCGGGAGGTTCCCAGAGAAGCCTTTCTCCCCTTGGGGATGGAACTTTTTGCCTACG
ACGACGGTCCGCTCCCCATAGCGGAGGGGCAGACCATCTCGCAGCCCTACATCGTCGCCTACATGATTGAGGCGCTCGAG
TTGCTGGGCAGGGAGCGGGTGCTCGAGATCGGCACCGGTTCAGGCTATGCCGCGGCGGTTCTTAGCCGTTGCGCCGCCCA
GGTCTACACGGTGGAGCGCATCCCCTCCCTGGCCGCGGGGGCGCGGGCGAGGCTCAAAGAGCTTGGCTACGGCAACGTCA
CCGTCCATCTCGGAGACGGCACACGGGGGTGGCTGGAACACGCGCCCTACGACGCCATTGTGGTGACTGCGGGGGCGCCC
GAGGTGCCGCGCGAGTTGCTGGACCAGCTGGCGCCCTTGGGGAGACTGGTGATACCGGTCGGCCCCACCCAGCACCTGCA
GGAGCTGGTGCTGGTGCGCCGGACGCGTGACGGGCATTTCCGCCATGAGCAGCTCTGCCCGGTTCGCTTCGTGCCGCTGA
TCGGCGAGCACGGCTGGTGA

Upstream 100 bases:

>100_bases
TGATGCCAGAATGGCGGCTGTTGAAATCGGGCTGTGACTGTCAGGCCAGCCCCGTTGTCTGCCGATTGCTGTATGTTACC
ATTTGCACCTTGGAGGAGGT

Downstream 100 bases:

>100_bases
GCTAGGCGGGGAGGGGGCCTGCTGCCGGCAGCTGGTGCATGGTGTCGAGCAGGTCCTTCTCGCTCACGGCGCTGTAGCGC
GACACGAGCCGCATCAACTG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTVDPKRAMEARKERMLSEHLAARGIRDAAVMQAMREVPREAFLPLGMELFAYDDGPLPIAEGQTISQPYIVAYMIEALE
LLGRERVLEIGTGSGYAAAVLSRCAAQVYTVERIPSLAAGARARLKELGYGNVTVHLGDGTRGWLEHAPYDAIVVTAGAP
EVPRELLDQLAPLGRLVIPVGPTQHLQELVLVRRTRDGHFRHEQLCPVRFVPLIGEHGW

Sequences:

>Translated_219_residues
MTVDPKRAMEARKERMLSEHLAARGIRDAAVMQAMREVPREAFLPLGMELFAYDDGPLPIAEGQTISQPYIVAYMIEALE
LLGRERVLEIGTGSGYAAAVLSRCAAQVYTVERIPSLAAGARARLKELGYGNVTVHLGDGTRGWLEHAPYDAIVVTAGAP
EVPRELLDQLAPLGRLVIPVGPTQHLQELVLVRRTRDGHFRHEQLCPVRFVPLIGEHGW
>Mature_218_residues
TVDPKRAMEARKERMLSEHLAARGIRDAAVMQAMREVPREAFLPLGMELFAYDDGPLPIAEGQTISQPYIVAYMIEALEL
LGRERVLEIGTGSGYAAAVLSRCAAQVYTVERIPSLAAGARARLKELGYGNVTVHLGDGTRGWLEHAPYDAIVVTAGAPE
VPRELLDQLAPLGRLVIPVGPTQHLQELVLVRRTRDGHFRHEQLCPVRFVPLIGEHGW

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=171, Percent_Identity=38.0116959064327, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1789100, Length=206, Percent_Identity=49.5145631067961, Blast_Score=176, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71983477, Length=197, Percent_Identity=31.9796954314721, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193207222, Length=195, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17981723, Length=222, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24132; Mature: 24001

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDPKRAMEARKERMLSEHLAARGIRDAAVMQAMREVPREAFLPLGMELFAYDDGPLPI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCC
AEGQTISQPYIVAYMIEALELLGRERVLEIGTGSGYAAAVLSRCAAQVYTVERIPSLAAG
CCCCCCCCHHHHHHHHHHHHHHCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARARLKELGYGNVTVHLGDGTRGWLEHAPYDAIVVTAGAPEVPRELLDQLAPLGRLVIPV
HHHHHHHCCCCCEEEEECCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEC
GPTQHLQELVLVRRTRDGHFRHEQLCPVRFVPLIGEHGW
CCHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCCC
>Mature Secondary Structure 
TVDPKRAMEARKERMLSEHLAARGIRDAAVMQAMREVPREAFLPLGMELFAYDDGPLPI
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCC
AEGQTISQPYIVAYMIEALELLGRERVLEIGTGSGYAAAVLSRCAAQVYTVERIPSLAAG
CCCCCCCCHHHHHHHHHHHHHHCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ARARLKELGYGNVTVHLGDGTRGWLEHAPYDAIVVTAGAPEVPRELLDQLAPLGRLVIPV
HHHHHHHCCCCCEEEEECCCCCHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEC
GPTQHLQELVLVRRTRDGHFRHEQLCPVRFVPLIGEHGW
CCHHHHHHHHHHHHCCCCCCCHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA