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The map label for this gene is tyrS [H]

Identifier: 197117360

GI number: 197117360

Start: 1121990

End: 1123201

Strand: Direct

Name: tyrS [H]

Synonym: Gbem_0970

Alternate gene names: 197117360

Gene position: 1121990-1123201 (Clockwise)

Preceding gene: 197117359

Following gene: 308535204

Centisome position: 24.31

GC content: 56.35

Gene sequence:

>1212_bases
ATGACCGTTGCAGAGCAGATGGAAATAATCAAGAGGGGAGCCGTAGAGATTCTGGTGGAGAAAGAGCTGGTTGAAAAGCT
CGAGAAATCCGTCAAGACCGGCGTGCCGCTCAAGATAAAGGCCGGTTTCGATCCGACCGCGCCCGATTTGCATCTGGGGC
ACACCGTGCTGTTGCACAAGATGCGTCAGTTCCAGAAGCTTGGCCACGAAGTCCACTTCCTGATCGGCGACTTCACAGGC
ATGATCGGCGACCCCACCGGCAAGTCCGAAACCCGCAAGGTGTTGACCCGCGAGGACGTGTTGAAGAACGCCGAGACCTA
CAAGGAGCAGGTCTTCAAGATCCTCGATCCCAAGCTCACCAAGGTGGTCTTCAACTCCGAATGGTTAGGCAAGCTCTCCG
CGTCCGACATGATCGGACTCGCCTCCAAGTACACCGTCGCCCGGATGCTCGAGCGCGAAGATTTCAGCAACCGTTTTTCC
AACCAGCTTCCCATCAGCATCCACGAATTCATGTATCCGCTGGTGCAGGGATACGATTCGGTGGCTCTCAAGGCCGACGT
CGAATTGGGCGGCACCGACCAGAAGTTCAACCTCCTGGTAGGGCGTGAACTGCAAAGGGAATGGGGGCAAGCGCCCCAGT
GCGTAATCACCATGCCGCTTCTTGAAGGACTCGACGGCGTGAACAAGATGAGCAAGTCCCTTGGGAACTACATAGGCATC
AACGAGCCCGCCGACGAGATCTACGGCAAGATCATGTCCATCTCCGATGAGCTGATGATTCGCTACTACGAGCTGTTGAG
CGACCTGACCATAGTCGAATTCGAGAAGCTCAAATCTGACCTGAAGGATGGGGCGATCCATCCTATGGAGGCGAAGAAGC
AGCTTGCCCGGGAAATGGTGGGCCGCTACCACGGCGTGGAGGCTGCCACCCTTGCCGACGAAAACTTCGTCAAGCGTTTC
AAGAACAACGAGACACCGGAAGATATGCCTGAGTTCACCCTGAAGGCTGAGGGGGAGAAGGTGCTTCTTTGCAAGGTGCT
TGCGGAGGCGCAGCTCACCAAGTCCAACAGCGAAGGGCGTCGCTCCATCCAGGGCGGCGGCGTTAAAGTCAACGGCGATA
AAGTCAGCGACGAAAACCTCGAAATAGCCTGTGTTGGAGAGTACGTTATCCAGGTAGGGAAGCGTCGTTTCGCCAAGGTG
CGGTTCGCCTAG

Upstream 100 bases:

>100_bases
GCATCAATCCCTCTCTTGAATACGCGGCTGACGCGTCCCGTCGCCCGGCGGGAGAGGGACAGGGTGAGGGCATTTTTGAA
ATAAATCTGGAGGGAAAGTG

Downstream 100 bases:

>100_bases
CTCTAAAAGACAATGTCAGACGATTGGGGGGGGCGATGCGCAAGCATCGCCCCTTCTGCGTGGAGATGCCGCCTGTCCAT
TAAGTCCACCAAGTCCATCG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTVAEQMEIIKRGAVEILVEKELVEKLEKSVKTGVPLKIKAGFDPTAPDLHLGHTVLLHKMRQFQKLGHEVHFLIGDFTG
MIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPKLTKVVFNSEWLGKLSASDMIGLASKYTVARMLEREDFSNRFS
NQLPISIHEFMYPLVQGYDSVALKADVELGGTDQKFNLLVGRELQREWGQAPQCVITMPLLEGLDGVNKMSKSLGNYIGI
NEPADEIYGKIMSISDELMIRYYELLSDLTIVEFEKLKSDLKDGAIHPMEAKKQLAREMVGRYHGVEAATLADENFVKRF
KNNETPEDMPEFTLKAEGEKVLLCKVLAEAQLTKSNSEGRRSIQGGGVKVNGDKVSDENLEIACVGEYVIQVGKRRFAKV
RFA

Sequences:

>Translated_403_residues
MTVAEQMEIIKRGAVEILVEKELVEKLEKSVKTGVPLKIKAGFDPTAPDLHLGHTVLLHKMRQFQKLGHEVHFLIGDFTG
MIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPKLTKVVFNSEWLGKLSASDMIGLASKYTVARMLEREDFSNRFS
NQLPISIHEFMYPLVQGYDSVALKADVELGGTDQKFNLLVGRELQREWGQAPQCVITMPLLEGLDGVNKMSKSLGNYIGI
NEPADEIYGKIMSISDELMIRYYELLSDLTIVEFEKLKSDLKDGAIHPMEAKKQLAREMVGRYHGVEAATLADENFVKRF
KNNETPEDMPEFTLKAEGEKVLLCKVLAEAQLTKSNSEGRRSIQGGGVKVNGDKVSDENLEIACVGEYVIQVGKRRFAKV
RFA
>Mature_402_residues
TVAEQMEIIKRGAVEILVEKELVEKLEKSVKTGVPLKIKAGFDPTAPDLHLGHTVLLHKMRQFQKLGHEVHFLIGDFTGM
IGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPKLTKVVFNSEWLGKLSASDMIGLASKYTVARMLEREDFSNRFSN
QLPISIHEFMYPLVQGYDSVALKADVELGGTDQKFNLLVGRELQREWGQAPQCVITMPLLEGLDGVNKMSKSLGNYIGIN
EPADEIYGKIMSISDELMIRYYELLSDLTIVEFEKLKSDLKDGAIHPMEAKKQLAREMVGRYHGVEAATLADENFVKRFK
NNETPEDMPEFTLKAEGEKVLLCKVLAEAQLTKSNSEGRRSIQGGGVKVNGDKVSDENLEIACVGEYVIQVGKRRFAKVR
FA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=431, Percent_Identity=26.2180974477958, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787925, Length=392, Percent_Identity=27.5510204081633, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=412, Percent_Identity=26.9417475728155, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6325160, Length=423, Percent_Identity=24.822695035461, Blast_Score=87, Evalue=6e-18,
Organism=Drosophila melanogaster, GI20130383, Length=410, Percent_Identity=25.1219512195122, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45258; Mature: 45127

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAEQMEIIKRGAVEILVEKELVEKLEKSVKTGVPLKIKAGFDPTAPDLHLGHTVLLHK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHH
MRQFQKLGHEVHFLIGDFTGMIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPKLT
HHHHHHHCCHHEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KVVFNSEWLGKLSASDMIGLASKYTVARMLEREDFSNRFSNQLPISIHEFMYPLVQGYDS
HHEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
VALKADVELGGTDQKFNLLVGRELQREWGQAPQCVITMPLLEGLDGVNKMSKSLGNYIGI
EEEEEECEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHCCCCCC
NEPADEIYGKIMSISDELMIRYYELLSDLTIVEFEKLKSDLKDGAIHPMEAKKQLAREMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GRYHGVEAATLADENFVKRFKNNETPEDMPEFTLKAEGEKVLLCKVLAEAQLTKSNSEGR
HHHCCCCHHHHCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHHCCCCCCC
RSIQGGGVKVNGDKVSDENLEIACVGEYVIQVGKRRFAKVRFA
HHCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVAEQMEIIKRGAVEILVEKELVEKLEKSVKTGVPLKIKAGFDPTAPDLHLGHTVLLHK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHH
MRQFQKLGHEVHFLIGDFTGMIGDPTGKSETRKVLTREDVLKNAETYKEQVFKILDPKLT
HHHHHHHCCHHEEHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KVVFNSEWLGKLSASDMIGLASKYTVARMLEREDFSNRFSNQLPISIHEFMYPLVQGYDS
HHEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCC
VALKADVELGGTDQKFNLLVGRELQREWGQAPQCVITMPLLEGLDGVNKMSKSLGNYIGI
EEEEEECEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCHHHHHHHHHCCCCCC
NEPADEIYGKIMSISDELMIRYYELLSDLTIVEFEKLKSDLKDGAIHPMEAKKQLAREMV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
GRYHGVEAATLADENFVKRFKNNETPEDMPEFTLKAEGEKVLLCKVLAEAQLTKSNSEGR
HHHCCCCHHHHCCHHHHHHHCCCCCCCCCCCCEEECCCCEEEHHHHHHHHHHHCCCCCCC
RSIQGGGVKVNGDKVSDENLEIACVGEYVIQVGKRRFAKVRFA
HHCCCCCEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA