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The map label for this gene is rpfG [H]

Identifier: 197117277

GI number: 197117277

Start: 1039239

End: 1040201

Strand: Direct

Name: rpfG [H]

Synonym: Gbem_0887

Alternate gene names: 197117277

Gene position: 1039239-1040201 (Clockwise)

Preceding gene: 197117276

Following gene: 197117278

Centisome position: 22.52

GC content: 57.01

Gene sequence:

>963_bases
ATGGGGGAGATGTACGTACCCGTCAGCATAGAAAGCATCCAGGCCGATCTTTTCCCGGATGTGGCCCTTTACATCAGAAG
TGGTGAAAAATACATCCTTTACAAGAGCCATGGCCGCAATTTCACCGCCAGCGACCAGGAACGGCTCATGGAAAACAAGG
TGGAGTTCCTCTTCGTAAGTCCGGCCGACCTGGAGGTGATCACGACCTTCATGGAGAACAACGCCGAGCGCGTGTTGAAG
GACAATACGCTGCAGGCGAAGACCAAGGGAAAGATCATCTACCAGACCTCCATCAACTTCGTGGGGGACATCTTCGAACA
CCCAGAGAAGGTCTCGGATTTTTCCCGTAGCAAGCGGCTGGTGGAAAACCTGCTGCAGTACCTGGCCAGCGACCGCGATT
CCTTCAATTCCCTGGAAAGCGTGATGGCCCACAACTACTACACCTTCGTGCACAGCCTGCAGGTGACCGCGCTGTCGGTG
CTGCTGCATGCGGAGGCCTACCTTCTGTCCCACGACGAAATGCTCGACGTCGGTATCGGGACTCTCCTGCACGATTTCGG
CAAGGTATTCGTTGCGCCAGCGATCCTGAACAACGAAGGGAAGCTCTCCAAGGCCGAGATCGAGGAATACCGCAAGCATC
CGGAAGAGGGGTACCAGTACCTCAAGAAAAACACCAGGCTTTCCGAGATCTCCCTCGGAGTCGTGCGCTGTCATCACGAG
CGCAACAACGGCAACGGGTATCCCCAGGGGCTGAAAGGGCCGGCCATTTCCCGCAGCGCGCAGGTCGCCGCCATCTGCGA
CGTCTACTGCACCTTAACCATCGACCGTGCCTGCCGTAAGGCCCTCAGTTCCGCCATTGCCGTCTCCATCATGAAGCAGG
AGATGAAGGGCTCGTTCAACGAGCGGCTGCTCGATGTACTGGAAGGGATCGTCTGCACCGAAAACCCGGCGCAGTTCTTG
TGA

Upstream 100 bases:

>100_bases
GGAAAAAGCCCTGCTGGCCCTTAAGCTCATGAGCGTGCTGCCGATATAGTTCCTGTGCCCACCAACGCAGGGGGGCAACG
GTAACAACGGAGGTGGCGCC

Downstream 100 bases:

>100_bases
ACCAATTGACAATTGACAATGGACAATTGACAACGAAATACAAACCACTACCACGCAGCGGGCAGTGTCCAGTCCAACTT
GAAGCATTGGGGATAATGAA

Product: cyclic diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGEMYVPVSIESIQADLFPDVALYIRSGEKYILYKSHGRNFTASDQERLMENKVEFLFVSPADLEVITTFMENNAERVLK
DNTLQAKTKGKIIYQTSINFVGDIFEHPEKVSDFSRSKRLVENLLQYLASDRDSFNSLESVMAHNYYTFVHSLQVTALSV
LLHAEAYLLSHDEMLDVGIGTLLHDFGKVFVAPAILNNEGKLSKAEIEEYRKHPEEGYQYLKKNTRLSEISLGVVRCHHE
RNNGNGYPQGLKGPAISRSAQVAAICDVYCTLTIDRACRKALSSAIAVSIMKQEMKGSFNERLLDVLEGIVCTENPAQFL

Sequences:

>Translated_320_residues
MGEMYVPVSIESIQADLFPDVALYIRSGEKYILYKSHGRNFTASDQERLMENKVEFLFVSPADLEVITTFMENNAERVLK
DNTLQAKTKGKIIYQTSINFVGDIFEHPEKVSDFSRSKRLVENLLQYLASDRDSFNSLESVMAHNYYTFVHSLQVTALSV
LLHAEAYLLSHDEMLDVGIGTLLHDFGKVFVAPAILNNEGKLSKAEIEEYRKHPEEGYQYLKKNTRLSEISLGVVRCHHE
RNNGNGYPQGLKGPAISRSAQVAAICDVYCTLTIDRACRKALSSAIAVSIMKQEMKGSFNERLLDVLEGIVCTENPAQFL
>Mature_319_residues
GEMYVPVSIESIQADLFPDVALYIRSGEKYILYKSHGRNFTASDQERLMENKVEFLFVSPADLEVITTFMENNAERVLKD
NTLQAKTKGKIIYQTSINFVGDIFEHPEKVSDFSRSKRLVENLLQYLASDRDSFNSLESVMAHNYYTFVHSLQVTALSVL
LHAEAYLLSHDEMLDVGIGTLLHDFGKVFVAPAILNNEGKLSKAEIEEYRKHPEEGYQYLKKNTRLSEISLGVVRCHHER
NNGNGYPQGLKGPAISRSAQVAAICDVYCTLTIDRACRKALSSAIAVSIMKQEMKGSFNERLLDVLEGIVCTENPAQFL

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 36074; Mature: 35942

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEMYVPVSIESIQADLFPDVALYIRSGEKYILYKSHGRNFTASDQERLMENKVEFLFVS
CCCEEEEEEHHHHHHHHCCHHEEEEECCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEC
PADLEVITTFMENNAERVLKDNTLQAKTKGKIIYQTSINFVGDIFEHPEKVSDFSRSKRL
CCCHHHHHHHHHCCHHHHHHCCCEEECCCCCEEEEECHHHHHHHHHCHHHHHHHHHHHHH
VENLLQYLASDRDSFNSLESVMAHNYYTFVHSLQVTALSVLLHAEAYLLSHDEMLDVGIG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLLHDFGKVFVAPAILNNEGKLSKAEIEEYRKHPEEGYQYLKKNTRLSEISLGVVRCHHE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHEEEEE
RNNGNGYPQGLKGPAISRSAQVAAICDVYCTLTIDRACRKALSSAIAVSIMKQEMKGSFN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERLLDVLEGIVCTENPAQFL
HHHHHHHCCCEECCCCCCCC
>Mature Secondary Structure 
GEMYVPVSIESIQADLFPDVALYIRSGEKYILYKSHGRNFTASDQERLMENKVEFLFVS
CCEEEEEEHHHHHHHHCCHHEEEEECCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEC
PADLEVITTFMENNAERVLKDNTLQAKTKGKIIYQTSINFVGDIFEHPEKVSDFSRSKRL
CCCHHHHHHHHHCCHHHHHHCCCEEECCCCCEEEEECHHHHHHHHHCHHHHHHHHHHHHH
VENLLQYLASDRDSFNSLESVMAHNYYTFVHSLQVTALSVLLHAEAYLLSHDEMLDVGIG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLLHDFGKVFVAPAILNNEGKLSKAEIEEYRKHPEEGYQYLKKNTRLSEISLGVVRCHHE
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHEEEEE
RNNGNGYPQGLKGPAISRSAQVAAICDVYCTLTIDRACRKALSSAIAVSIMKQEMKGSFN
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERLLDVLEGIVCTENPAQFL
HHHHHHHCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]