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The map label for this gene is rpoN [H]

Identifier: 197117261

GI number: 197117261

Start: 1024256

End: 1025803

Strand: Direct

Name: rpoN [H]

Synonym: Gbem_0869

Alternate gene names: 197117261

Gene position: 1024256-1025803 (Clockwise)

Preceding gene: 197117260

Following gene: 197117262

Centisome position: 22.19

GC content: 61.89

Gene sequence:

>1548_bases
ATGGCAATAGAGATGCGCCAGCAGATGAAAATGAGTCAGCAACTGGTGATGACGCCCCAGTTGCAGCAGGCCATCAAGCT
CCTCCAGCTTTCCCGGCTGGAGTTGCAGGACGTAGTACGTCAGGAGCTGGAGGAGAACCCCATACTCGACGAGGTGATCG
AGCAGGAGGAGATCCGGGAACCCGAGCAGATCGAGTTGCGCGAGAAGGAAGCCGAGCCGGAGGCCGCAGCGAGCGATTTC
CAGGAAGTGCGGGCCGGCGAGGAGACGCGCGAGGCGGACTGGGATTCCTACATAGACGGCTACAACTACAGCTCCGGCGA
GCAGTACTACGACGACGAGGACCGTCCCTCCTTCGAGAACCTCCTCACCAAGAAATCCACCCTGTTCGACCACCTGATGT
GGCAGCTGAGCCTCACCCGCCTCACCGAGCGCGAGATTGTGGTAGGAGCCGAAATCATCGGCAACATCGACGAGGAGGGC
TACCTCCGCGCCTCCCTCGAGGACGTAGCTTCCGCCTGCGTGCAGGTTGCCCCGTTTCAGGAAGAGATGCTCGAGTGGTC
GGGGCTCGGCTCTGACGCCTGCGAGGAAGAGATCGCCGATGCTGCGGGCGGTTTCTCCACTGCCGTGCTGGTTCCGCTGG
TCGATTCGGTGTTGAAGCGGATCCACGAATTCGACCCGGTCGGCGTCGGGGCTCGCGACCTGCGCGAGTGCCTCCTGATC
CAGGTCGGCAGCCTCGGCATGGGGGGGAGCCTCGTGGAGTCGCTGTTGCGCGACCACCTTAAGGACCTGGAGAGCCACAA
GTACAAGCAGGCCGCCAAGGTGCTGGGGGTGGAGGTGAACGACATCCTCGCCGCCACGAGGATCATCGCGGAACTCGATC
CCAAGCCGGGGCGGGTCTTTGGCAGCGACGACGTGCAGTACATCTCGGCCGACATCTTCGTGCACAAGGTGGGTGAAGAG
TACGTGGTGATGCTGAACGACGAGGGGATGCCCAACTTGAGGATCAACCCCATCTACGCCCCCGACGCCAAGAGCAGCCG
CCCGGTGGACAAGGTGGCCGAGGATTACATCGGCGAGAAGATGCGCTCCGCCCTGTGGCTCATCAAGAGCATCCAGCAGC
GCCAGCGCACCATCTTCAAGGTGGCCAAGAGCATCGTGAAGTTCCAGCGCGAATTTCTGGACCGCGGCATCGAGCACCTG
CGCCCGCTGGTCCTGAGGGACATCGCCGAGGACATCGGCATGCACGAGTCCACCATCAGCCGTGTCACCACCAACAAGTA
CATGCAGACCCCGCAGGGGCTCTTCGAGCTGAAGTACTTCTTCAACTCCGGCATCTCGACCGGGGAGGGGGACTTCATCG
CCTCCGAGAGCGTGAAGAGCAAGATCAAGGAACTGGTGGACAACGAGGACTCCAAGCGCCCCTACAGCGACCAGCGCCTG
GCGGAACTCCTCTCGGACCACAACATCGTCATAGCCCGCCGCACCGTTACCAAGTATCGCGAGATGCTTCGGATCGGCTC
GTCTTCGGAGCGCAAGAAGCATTTCTAA

Upstream 100 bases:

>100_bases
TAACTGTTAAACCGGCAACTAACAACGCATAAACAGCACGTAGAACTTCATCCGCTTAGAAAGGAACTGTCGTAGCAGTA
CACGGATTCAGCGAGGACTT

Downstream 100 bases:

>100_bases
ACGCGGGCGCCGCCGTCGGCCGGCGCGTGAGCCGCGTGTATTGACAATAAAGGGAGGTACTTATTATGCAAACAACCGTG
ACGTTCAGACACATGGAGCC

Product: RNA polymerase sigma-54 factor RpoN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MAIEMRQQMKMSQQLVMTPQLQQAIKLLQLSRLELQDVVRQELEENPILDEVIEQEEIREPEQIELREKEAEPEAAASDF
QEVRAGEETREADWDSYIDGYNYSSGEQYYDDEDRPSFENLLTKKSTLFDHLMWQLSLTRLTEREIVVGAEIIGNIDEEG
YLRASLEDVASACVQVAPFQEEMLEWSGLGSDACEEEIADAAGGFSTAVLVPLVDSVLKRIHEFDPVGVGARDLRECLLI
QVGSLGMGGSLVESLLRDHLKDLESHKYKQAAKVLGVEVNDILAATRIIAELDPKPGRVFGSDDVQYISADIFVHKVGEE
YVVMLNDEGMPNLRINPIYAPDAKSSRPVDKVAEDYIGEKMRSALWLIKSIQQRQRTIFKVAKSIVKFQREFLDRGIEHL
RPLVLRDIAEDIGMHESTISRVTTNKYMQTPQGLFELKYFFNSGISTGEGDFIASESVKSKIKELVDNEDSKRPYSDQRL
AELLSDHNIVIARRTVTKYREMLRIGSSSERKKHF

Sequences:

>Translated_515_residues
MAIEMRQQMKMSQQLVMTPQLQQAIKLLQLSRLELQDVVRQELEENPILDEVIEQEEIREPEQIELREKEAEPEAAASDF
QEVRAGEETREADWDSYIDGYNYSSGEQYYDDEDRPSFENLLTKKSTLFDHLMWQLSLTRLTEREIVVGAEIIGNIDEEG
YLRASLEDVASACVQVAPFQEEMLEWSGLGSDACEEEIADAAGGFSTAVLVPLVDSVLKRIHEFDPVGVGARDLRECLLI
QVGSLGMGGSLVESLLRDHLKDLESHKYKQAAKVLGVEVNDILAATRIIAELDPKPGRVFGSDDVQYISADIFVHKVGEE
YVVMLNDEGMPNLRINPIYAPDAKSSRPVDKVAEDYIGEKMRSALWLIKSIQQRQRTIFKVAKSIVKFQREFLDRGIEHL
RPLVLRDIAEDIGMHESTISRVTTNKYMQTPQGLFELKYFFNSGISTGEGDFIASESVKSKIKELVDNEDSKRPYSDQRL
AELLSDHNIVIARRTVTKYREMLRIGSSSERKKHF
>Mature_514_residues
AIEMRQQMKMSQQLVMTPQLQQAIKLLQLSRLELQDVVRQELEENPILDEVIEQEEIREPEQIELREKEAEPEAAASDFQ
EVRAGEETREADWDSYIDGYNYSSGEQYYDDEDRPSFENLLTKKSTLFDHLMWQLSLTRLTEREIVVGAEIIGNIDEEGY
LRASLEDVASACVQVAPFQEEMLEWSGLGSDACEEEIADAAGGFSTAVLVPLVDSVLKRIHEFDPVGVGARDLRECLLIQ
VGSLGMGGSLVESLLRDHLKDLESHKYKQAAKVLGVEVNDILAATRIIAELDPKPGRVFGSDDVQYISADIFVHKVGEEY
VVMLNDEGMPNLRINPIYAPDAKSSRPVDKVAEDYIGEKMRSALWLIKSIQQRQRTIFKVAKSIVKFQREFLDRGIEHLR
PLVLRDIAEDIGMHESTISRVTTNKYMQTPQGLFELKYFFNSGISTGEGDFIASESVKSKIKELVDNEDSKRPYSDQRLA
ELLSDHNIVIARRTVTKYREMLRIGSSSERKKHF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=512, Percent_Identity=38.28125, Blast_Score=330, Evalue=2e-91,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 58555; Mature: 58424

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEMRQQMKMSQQLVMTPQLQQAIKLLQLSRLELQDVVRQELEENPILDEVIEQEEIRE
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
PEQIELREKEAEPEAAASDFQEVRAGEETREADWDSYIDGYNYSSGEQYYDDEDRPSFEN
CHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHH
LLTKKSTLFDHLMWQLSLTRLTEREIVVGAEIIGNIDEEGYLRASLEDVASACVQVAPFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCH
EEMLEWSGLGSDACEEEIADAAGGFSTAVLVPLVDSVLKRIHEFDPVGVGARDLRECLLI
HHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QVGSLGMGGSLVESLLRDHLKDLESHKYKQAAKVLGVEVNDILAATRIIAELDPKPGRVF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEE
GSDDVQYISADIFVHKVGEEYVVMLNDEGMPNLRINPIYAPDAKSSRPVDKVAEDYIGEK
CCCCHHHHHHHHHHEECCCCEEEEECCCCCCCCEECCEECCCCCCCCCHHHHHHHHHHHH
MRSALWLIKSIQQRQRTIFKVAKSIVKFQREFLDRGIEHLRPLVLRDIAEDIGMHESTIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RVTTNKYMQTPQGLFELKYFFNSGISTGEGDFIASESVKSKIKELVDNEDSKRPYSDQRL
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
AELLSDHNIVIARRTVTKYREMLRIGSSSERKKHF
HHHHCCCCEEEEHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
AIEMRQQMKMSQQLVMTPQLQQAIKLLQLSRLELQDVVRQELEENPILDEVIEQEEIRE
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCC
PEQIELREKEAEPEAAASDFQEVRAGEETREADWDSYIDGYNYSSGEQYYDDEDRPSFEN
CHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCCHHHHHCCCCCCCCCCCCCCCCCCHHHH
LLTKKSTLFDHLMWQLSLTRLTEREIVVGAEIIGNIDEEGYLRASLEDVASACVQVAPFQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCH
EEMLEWSGLGSDACEEEIADAAGGFSTAVLVPLVDSVLKRIHEFDPVGVGARDLRECLLI
HHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
QVGSLGMGGSLVESLLRDHLKDLESHKYKQAAKVLGVEVNDILAATRIIAELDPKPGRVF
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEE
GSDDVQYISADIFVHKVGEEYVVMLNDEGMPNLRINPIYAPDAKSSRPVDKVAEDYIGEK
CCCCHHHHHHHHHHEECCCCEEEEECCCCCCCCEECCEECCCCCCCCCHHHHHHHHHHHH
MRSALWLIKSIQQRQRTIFKVAKSIVKFQREFLDRGIEHLRPLVLRDIAEDIGMHESTIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RVTTNKYMQTPQGLFELKYFFNSGISTGEGDFIASESVKSKIKELVDNEDSKRPYSDQRL
HHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
AELLSDHNIVIARRTVTKYREMLRIGSSSERKKHF
HHHHCCCCEEEEHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]