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The map label for this gene is metH [H]

Identifier: 197116677

GI number: 197116677

Start: 334026

End: 336461

Strand: Direct

Name: metH [H]

Synonym: Gbem_0277

Alternate gene names: 197116677

Gene position: 334026-336461 (Clockwise)

Preceding gene: 197116676

Following gene: 197116678

Centisome position: 7.24

GC content: 66.38

Gene sequence:

>2436_bases
ATGCCTTTTATGCAGGCGGTTAAAGAGCGGGTGCTGGTTCTGGACGGCGCCATGGGGACCATGCTGCAGGAACGCGGGCT
GAAGCCGGGGCAGTCCCCGGAGGAGATGAACCTCACCGCAGCCGACGTGGTCGCCGGGGTGCACCAGGCTTATCTGGACG
CCGGCGCCGACATCATCGTCACCAACACCTTCGGCGGGACCAAGGCGAAGCTGGAACATTACGGCCTCGGCGACCAAGTG
GCGCGCATCAACGCCGAGGCGGTGAGGATCGCCCGCGAGGTGGCTGGCGAAAAGGCCTACGTGGCAGGTTCCATCGGCCC
CACCGGCCGCTTCCTGGAGCCGGTCGGCGACATGAGCTTCGACGAGGCAGTCTCGCTTTTCCGCGAGCAGGCACAGGCGC
TCGTCGACGCCGGCTGCGACCTGATCAGCTTCGAAACCTTCCTCGACATCAAGGAGATCCGCGCCGGTGTCATCGCGGTG
CGCGAGATCTCCAAGGAGATCCCGGTCATCGCCATGCTCACCTTCGAGGAGAAGGGGAGAAGCGTCCTCGGCACCCCGCC
CGAGGCTGCCGCCATCACCCTGGAGGCGGTGGGCGCGACCATCGTCGGCTCCAACTGCGGCTTAGGCCCTGAAGGGATCT
ACCAGATCCTCTCCGGCATGAGGGAGGTCACCGCGCTCCCGCTCATCTCCCAGGCGAACGCGGGGCTCCCGATCCTCAAG
GACGGAGTCACAATCTTCCCGGCGACCCCGGACGAGATGACTTCCTACCACGACCGGCTGATCGAACTCGGCGTCCGCAT
CATCGGCGGCTGCTGCGGCACCACCCCCGCCCACATCGCCGCCATCAAGAAAGCGCTCGCCGCGAAGCAGACTCCCTTCG
TCCCCAAGAAGGAGGACGGCACCACCTGGCTCTCCAGCCGCGGCAGCTTCGCCGCAGTCGGCTCCGCCCATCCGGTGGCG
CTCATCGGCGAGCGCATCAACCCGACCGGCAAAAAGCTCTACTCGCAGGAACTCAGGGAAGGGAAGGTGAGCTACATCCG
GCGCGAGGCGCTGGAGCAGACCGGGCTCGGCGCGACGCTTCTCGACGTGAACGTCGGCACCCCGGGGATCGACGAGCCGG
CCGCCATGGAACGCGCCGTCTTCTGCATCACCGGCGCAGTCCAGACGCCGCTGGTACTCGACTCATCCTCCCCTGCCGCC
CTGGAGGCGGGGCTTAAGGCCGCCGACGGCAAGGTGCTCATCAACTCGGTGAACGGCGAAGAAAAGAGCCTCGCCGCCGT
CCTCCCCCTGGCCAAGAAGTACGGCGCGGCGCTGGTCTGCCTCACGCTGGACGAGGCGGGGATCCCTGCGGAGGCTGCGG
GGCGGGTCGCCGTCGCCGAGAAGATCGCCGCTGCCGCTAAGGCCGCAGGGGTGAAAAGAAGCGATCTCGTGGTCGACTGC
CTCACCCTGACGGTGAGCGCCGAGCCCAAGGGGGCGCTGGTGGCGCTGGAAGCGGTGCGCCAGGTGAGCGCGCTCGGGCT
CAACACGACGCTCGGCGTTTCCAACATCTCCTTCGGCCTTCCCTGCCGCCCCCTCATCTCCTCCACCTTCTTCTCCTTGG
CCATGGCCGCCGGGCTCACCTCGGCCATCGTCAACGTGAAGGAGGCGCCGATGATGGCGGCCTGGAGAAGCTCCATGGTG
CTGCTGGAAAAGGACGTCAACGCGGCCTGTTACATCGAGGCCTACAAGGGGCAGGCGGTCGGCGCCACGGTGGAGCCAGC
CGCTGCCGCCGGCGCAACCGGAACCCGGGTCGCAGCCGTCGAGCCGGAAGGTGTCCGCGGGCGCCTGGCCAAGGCGGTCA
TCAACGGCGAAGCGGACAACGTGGTGGCGCTGGTGGAAGAGGCGCTGGCCGAGGGGCTGACCCCGATGCAGATCAGCTCC
GAGGCGCTCCTGGTCGGGCTCGACGAGGTGGGAAAGCGTTTCGGCTCCGGTGAATTCTTCCTGCCGCAGGTGATGGTCTC
AGCCGACACCATGAAGACCGCTTTCGCCCGGCTGAAGATGGAACTTTCCACCGGCGGATTGCAGAGCATCGGCAAGATCC
TGATGGCGACCGTCGAGGGGGACATTCACGACATCGGCAAGAACATCCTGGTCACCCTCCTGGAGAACAACGGTTTCGAG
GTGATAGATCTGGGCAAGAACGTCCCGGCCGACCGCATCCTGTACGAGGCGCGTGCCCACCAGGTGGATGCCGTCGGGCT
CTCCGCGCTGATGACGACGACCATGGCGCAGATGGACAAGGTGGTCAAACTCCTGAAGGCCGAAGGGGTGAAGAGCTTCA
CCATGGTCGGCGGGGCCGTGGTCACCCAGGAATACGCCGACGAGATCGGCGCCGACCTCTACGCCAAGGACGCCATGGAG
GCGGTAGCCCGCATCAAGAAACTGCTGGCAAAATAA

Upstream 100 bases:

>100_bases
GCAGCCGCCGTACCTCCTCCGGTGGCCGGACGGGGTGAGCGCGTCGAACGTTGAACTTTGAACCTTGAACCTTGAACGGA
TTTTGGGAGGTTAGATGAAG

Downstream 100 bases:

>100_bases
AGCTTTTCGGCGGCAACCCGGCTGGGGGAGCGCCCTAATATCGCTTGCAACGATCCTTCTGCTGTGCTAACTTGCGGTCG
GGTTCCGCCATTTGGCGTAG

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MPFMQAVKERVLVLDGAMGTMLQERGLKPGQSPEEMNLTAADVVAGVHQAYLDAGADIIVTNTFGGTKAKLEHYGLGDQV
ARINAEAVRIAREVAGEKAYVAGSIGPTGRFLEPVGDMSFDEAVSLFREQAQALVDAGCDLISFETFLDIKEIRAGVIAV
REISKEIPVIAMLTFEEKGRSVLGTPPEAAAITLEAVGATIVGSNCGLGPEGIYQILSGMREVTALPLISQANAGLPILK
DGVTIFPATPDEMTSYHDRLIELGVRIIGGCCGTTPAHIAAIKKALAAKQTPFVPKKEDGTTWLSSRGSFAAVGSAHPVA
LIGERINPTGKKLYSQELREGKVSYIRREALEQTGLGATLLDVNVGTPGIDEPAAMERAVFCITGAVQTPLVLDSSSPAA
LEAGLKAADGKVLINSVNGEEKSLAAVLPLAKKYGAALVCLTLDEAGIPAEAAGRVAVAEKIAAAAKAAGVKRSDLVVDC
LTLTVSAEPKGALVALEAVRQVSALGLNTTLGVSNISFGLPCRPLISSTFFSLAMAAGLTSAIVNVKEAPMMAAWRSSMV
LLEKDVNAACYIEAYKGQAVGATVEPAAAAGATGTRVAAVEPEGVRGRLAKAVINGEADNVVALVEEALAEGLTPMQISS
EALLVGLDEVGKRFGSGEFFLPQVMVSADTMKTAFARLKMELSTGGLQSIGKILMATVEGDIHDIGKNILVTLLENNGFE
VIDLGKNVPADRILYEARAHQVDAVGLSALMTTTMAQMDKVVKLLKAEGVKSFTMVGGAVVTQEYADEIGADLYAKDAME
AVARIKKLLAK

Sequences:

>Translated_811_residues
MPFMQAVKERVLVLDGAMGTMLQERGLKPGQSPEEMNLTAADVVAGVHQAYLDAGADIIVTNTFGGTKAKLEHYGLGDQV
ARINAEAVRIAREVAGEKAYVAGSIGPTGRFLEPVGDMSFDEAVSLFREQAQALVDAGCDLISFETFLDIKEIRAGVIAV
REISKEIPVIAMLTFEEKGRSVLGTPPEAAAITLEAVGATIVGSNCGLGPEGIYQILSGMREVTALPLISQANAGLPILK
DGVTIFPATPDEMTSYHDRLIELGVRIIGGCCGTTPAHIAAIKKALAAKQTPFVPKKEDGTTWLSSRGSFAAVGSAHPVA
LIGERINPTGKKLYSQELREGKVSYIRREALEQTGLGATLLDVNVGTPGIDEPAAMERAVFCITGAVQTPLVLDSSSPAA
LEAGLKAADGKVLINSVNGEEKSLAAVLPLAKKYGAALVCLTLDEAGIPAEAAGRVAVAEKIAAAAKAAGVKRSDLVVDC
LTLTVSAEPKGALVALEAVRQVSALGLNTTLGVSNISFGLPCRPLISSTFFSLAMAAGLTSAIVNVKEAPMMAAWRSSMV
LLEKDVNAACYIEAYKGQAVGATVEPAAAAGATGTRVAAVEPEGVRGRLAKAVINGEADNVVALVEEALAEGLTPMQISS
EALLVGLDEVGKRFGSGEFFLPQVMVSADTMKTAFARLKMELSTGGLQSIGKILMATVEGDIHDIGKNILVTLLENNGFE
VIDLGKNVPADRILYEARAHQVDAVGLSALMTTTMAQMDKVVKLLKAEGVKSFTMVGGAVVTQEYADEIGADLYAKDAME
AVARIKKLLAK
>Mature_810_residues
PFMQAVKERVLVLDGAMGTMLQERGLKPGQSPEEMNLTAADVVAGVHQAYLDAGADIIVTNTFGGTKAKLEHYGLGDQVA
RINAEAVRIAREVAGEKAYVAGSIGPTGRFLEPVGDMSFDEAVSLFREQAQALVDAGCDLISFETFLDIKEIRAGVIAVR
EISKEIPVIAMLTFEEKGRSVLGTPPEAAAITLEAVGATIVGSNCGLGPEGIYQILSGMREVTALPLISQANAGLPILKD
GVTIFPATPDEMTSYHDRLIELGVRIIGGCCGTTPAHIAAIKKALAAKQTPFVPKKEDGTTWLSSRGSFAAVGSAHPVAL
IGERINPTGKKLYSQELREGKVSYIRREALEQTGLGATLLDVNVGTPGIDEPAAMERAVFCITGAVQTPLVLDSSSPAAL
EAGLKAADGKVLINSVNGEEKSLAAVLPLAKKYGAALVCLTLDEAGIPAEAAGRVAVAEKIAAAAKAAGVKRSDLVVDCL
TLTVSAEPKGALVALEAVRQVSALGLNTTLGVSNISFGLPCRPLISSTFFSLAMAAGLTSAIVNVKEAPMMAAWRSSMVL
LEKDVNAACYIEAYKGQAVGATVEPAAAAGATGTRVAAVEPEGVRGRLAKAVINGEADNVVALVEEALAEGLTPMQISSE
ALLVGLDEVGKRFGSGEFFLPQVMVSADTMKTAFARLKMELSTGGLQSIGKILMATVEGDIHDIGKNILVTLLENNGFEV
IDLGKNVPADRILYEARAHQVDAVGLSALMTTTMAQMDKVVKLLKAEGVKSFTMVGGAVVTQEYADEIGADLYAKDAMEA
VARIKKLLAK

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=855, Percent_Identity=30.4093567251462, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI13162290, Length=316, Percent_Identity=30.6962025316456, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI157266337, Length=319, Percent_Identity=29.4670846394984, Blast_Score=102, Evalue=2e-21,
Organism=Escherichia coli, GI1790450, Length=881, Percent_Identity=30.4199772985244, Blast_Score=322, Evalue=5e-89,
Organism=Escherichia coli, GI1786456, Length=304, Percent_Identity=31.25, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17535405, Length=850, Percent_Identity=31.1764705882353, Blast_Score=319, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6324982, Length=321, Percent_Identity=27.7258566978193, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322966, Length=310, Percent_Identity=27.4193548387097, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 84564; Mature: 84433

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFMQAVKERVLVLDGAMGTMLQERGLKPGQSPEEMNLTAADVVAGVHQAYLDAGADIIV
CCHHHHHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
TNTFGGTKAKLEHYGLGDQVARINAEAVRIAREVAGEKAYVAGSIGPTGRFLEPVGDMSF
EECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCCCCH
DEAVSLFREQAQALVDAGCDLISFETFLDIKEIRAGVIAVREISKEIPVIAMLTFEEKGR
HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
SVLGTPPEAAAITLEAVGATIVGSNCGLGPEGIYQILSGMREVTALPLISQANAGLPILK
EECCCCCCHHEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEE
DGVTIFPATPDEMTSYHDRLIELGVRIIGGCCGTTPAHIAAIKKALAAKQTPFVPKKEDG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TTWLSSRGSFAAVGSAHPVALIGERINPTGKKLYSQELREGKVSYIRREALEQTGLGATL
CHHHCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LDVNVGTPGIDEPAAMERAVFCITGAVQTPLVLDSSSPAALEAGLKAADGKVLINSVNGE
EEEECCCCCCCCCHHHHHEEEEEECCCCCCEEECCCCCHHHHHCCCCCCCEEEEEECCCC
EKSLAAVLPLAKKYGAALVCLTLDEAGIPAEAAGRVAVAEKIAAAAKAAGVKRSDLVVDC
HHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHEEEE
LTLTVSAEPKGALVALEAVRQVSALGLNTTLGVSNISFGLPCRPLISSTFFSLAMAAGLT
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SAIVNVKEAPMMAAWRSSMVLLEKDVNAACYIEAYKGQAVGATVEPAAAAGATGTRVAAV
HHHHHHHCCCHHHHHHCCEEEEEECCCEEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEE
EPEGVRGRLAKAVINGEADNVVALVEEALAEGLTPMQISSEALLVGLDEVGKRFGSGEFF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHCCCCEE
LPQVMVSADTMKTAFARLKMELSTGGLQSIGKILMATVEGDIHDIGKNILVTLLENNGFE
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHHHCHHHEEEEECCCCEE
VIDLGKNVPADRILYEARAHQVDAVGLSALMTTTMAQMDKVVKLLKAEGVKSFTMVGGAV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHH
VTQEYADEIGADLYAKDAMEAVARIKKLLAK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFMQAVKERVLVLDGAMGTMLQERGLKPGQSPEEMNLTAADVVAGVHQAYLDAGADIIV
CHHHHHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEE
TNTFGGTKAKLEHYGLGDQVARINAEAVRIAREVAGEKAYVAGSIGPTGRFLEPVGDMSF
EECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCCCCH
DEAVSLFREQAQALVDAGCDLISFETFLDIKEIRAGVIAVREISKEIPVIAMLTFEEKGR
HHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
SVLGTPPEAAAITLEAVGATIVGSNCGLGPEGIYQILSGMREVTALPLISQANAGLPILK
EECCCCCCHHEEEEEECCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEE
DGVTIFPATPDEMTSYHDRLIELGVRIIGGCCGTTPAHIAAIKKALAAKQTPFVPKKEDG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
TTWLSSRGSFAAVGSAHPVALIGERINPTGKKLYSQELREGKVSYIRREALEQTGLGATL
CHHHCCCCCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LDVNVGTPGIDEPAAMERAVFCITGAVQTPLVLDSSSPAALEAGLKAADGKVLINSVNGE
EEEECCCCCCCCCHHHHHEEEEEECCCCCCEEECCCCCHHHHHCCCCCCCEEEEEECCCC
EKSLAAVLPLAKKYGAALVCLTLDEAGIPAEAAGRVAVAEKIAAAAKAAGVKRSDLVVDC
HHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHCCCCCHHHEEEE
LTLTVSAEPKGALVALEAVRQVSALGLNTTLGVSNISFGLPCRPLISSTFFSLAMAAGLT
EEEEEECCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SAIVNVKEAPMMAAWRSSMVLLEKDVNAACYIEAYKGQAVGATVEPAAAAGATGTRVAAV
HHHHHHHCCCHHHHHHCCEEEEEECCCEEEEEEEECCCCCCCCCCCHHHCCCCCCEEEEE
EPEGVRGRLAKAVINGEADNVVALVEEALAEGLTPMQISSEALLVGLDEVGKRFGSGEFF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCEEEEHHHHHHHHCCCCEE
LPQVMVSADTMKTAFARLKMELSTGGLQSIGKILMATVEGDIHDIGKNILVTLLENNGFE
HHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCCHHHHHCHHHEEEEECCCCEE
VIDLGKNVPADRILYEARAHQVDAVGLSALMTTTMAQMDKVVKLLKAEGVKSFTMVGGAV
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHH
VTQEYADEIGADLYAKDAMEAVARIKKLLAK
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]