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The map label for this gene is polX [H]

Identifier: 197116665

GI number: 197116665

Start: 313029

End: 314765

Strand: Direct

Name: polX [H]

Synonym: Gbem_0265

Alternate gene names: 197116665

Gene position: 313029-314765 (Clockwise)

Preceding gene: 197116664

Following gene: 308535153

Centisome position: 6.78

GC content: 63.15

Gene sequence:

>1737_bases
ATGAAAAACAGGGAAATCGCACGGATCTTTGCCGAGATCGCCGACATCCTGGAGATCAAGGAAGGGAACGTCTTCAAGAT
AAGGGCCTACCGGCGCGCGGCGCTCAACCTGGACGGCTTCAGCAGGGACCTGGCGCAGCTCTCCCACAAGGAACTCCTGG
AGATTCCGGGGGTGGGGGCTGACCTTGCCGGGAGGATAGAGGAATACCTCCAGACCGGCACCATGGCTGCCTACGAGGAG
TTGAAACAGGAGGTCCCCGCCGGCGTCTTCGCGCTATTGGCCATCCCGGACCTGGGCCCTAAGACGGCGAAGGCGATCTA
CGACGCCCTGCAGATAGCGAGCATCGAGGAGCTGGAAAAGGCCGCACTCGAACACAAGCTGATCGGCATCAAGGGGATTA
AGCAGAAGACGGAGGAGAACATCCTCAAGGGGATAGCGGCGGTGAAGCGCGGGCGCGAGCGCCAGCCGCTGGGGCGCATG
CTCCCTGCGGCGCAGGACCTGGTGGAGGCGCTCAAAGAGCGGGCTCCGGTGGAGCGGATCGAGGTCGCCGGAAGCATCCG
GAGGCGCAGGGACAGCATCAAGGACATCGACATTGTCGCCACCTCGCCCGATCCGGCCGCCGTCATGGCGGCTTTTGTCG
ATTTGCCCCAGGTGCACGACGTTATCATGCGCGGCCCGACCCGTGCCAGCGTCACTATTCGCGAGGGGGTGCAGGTGGAC
CTGCGCGTGGTGGATCCTGCCTCCTATGGCGCCGCCCTTGCCTACCTCACCGGCAGCCAGGCCCACAACGTGCGCTTGCG
CGAGATGGCCCAGAAACGGGGGCTGAAGATCAACGAATACGGGATCTTCCGTGAGGAGGACAACCAGCGCCTGGGGGGCG
TGGACGAGGAAGACGTCTACCGCCTGCTGGACCTGGCCTTTGTCCCCCCGGTGCTGCGTGAGGACCGCGGCGAGATCGAA
GCGGCCCTTTTGGGGAAGCTGCCGCAGCTGGTGACCCAGGCCGATATCAGGGGGGATCTGCACGTCCACTCCAGGTGGAG
CGACGGCGCCCACGGCATCGCCGAGCTGGTGGAGGCGGCAAGGGAACGAGGCCTGTCCTACATCGCGGTAACCGATCATT
CGCAGGGGCTAGGAGTGGCCCGCGGTCTTACGGTGGAGCGGCTTCGGGAACAGGCCGCGGAGATAAAGGAATTGAACCGG
GAGCTCAAGGGATTCCGGGTCCTGCACGGCACCGAGATGGACATCCTGGGGGATGGAGCGCTCGATTTTCCCGACGAGGT
GCTGAAGGGTCTCGACATAGTAGTCGCCTCCATCCACTCCGGGTTCAACAACTCGAAGGAAGTCATGACCTCGCGCATAG
TGGCCGCCATGCGTAACCCCTACATCTCGATCATCGGGCATCCGACCGGGCGCATCCTAGGAGAGAGGGAAGGGTACCAG
ATCGACATGGACGAGGTGCTGCGGGTAGCCAAGGAGACCGGGACGGCGCTGGAGATAAACGCCTACCCGATGCGGCTGGA
CCTGGAGGATAAGCACGTGCGCCGCGCCAAGGAGCTGGGCATCATGATAGCCGTCAACACCGACACCCATGCCAAGCTGC
AATTCGATTTTCTCCCCTACGGGATCTCGGTCGCCCAGCGCGGCTGGCTCGAACCTGCGGACGTGCTGAACACGCTGGAT
CCCGACCAGCTGCTGAAGAAGCTCAGAGAGAAAAGGAAGAGGATGGCTCGAAAATAA

Upstream 100 bases:

>100_bases
ACTACTACAAGAAGAACCCGATCCGCTACTTCTACTACCGCTCGAGCTGCGGGCGCGACCGGCGGCTGAAGGAGCTGTGG
GGGCGTTAACGAGAGAGGCT

Downstream 100 bases:

>100_bases
AGGTCATCGGGAAACGTCGCTCTAGGTCCCCCCTCCCCCGGTGGGAGGGGGTTAGGGGGTGGGGGTAGGTGCCACCTTTT
GAGCTGCTGCCAAGTTCACC

Product: DNA polymerase IV domain-/PHP phosphoesterase domain-containing protein

Products: NA

Alternate protein names: DNA polymerase type-X; 3'-5' exodeoxyribonuclease; 3'-5' exonuclease [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKNREIARIFAEIADILEIKEGNVFKIRAYRRAALNLDGFSRDLAQLSHKELLEIPGVGADLAGRIEEYLQTGTMAAYEE
LKQEVPAGVFALLAIPDLGPKTAKAIYDALQIASIEELEKAALEHKLIGIKGIKQKTEENILKGIAAVKRGRERQPLGRM
LPAAQDLVEALKERAPVERIEVAGSIRRRRDSIKDIDIVATSPDPAAVMAAFVDLPQVHDVIMRGPTRASVTIREGVQVD
LRVVDPASYGAALAYLTGSQAHNVRLREMAQKRGLKINEYGIFREEDNQRLGGVDEEDVYRLLDLAFVPPVLREDRGEIE
AALLGKLPQLVTQADIRGDLHVHSRWSDGAHGIAELVEAARERGLSYIAVTDHSQGLGVARGLTVERLREQAAEIKELNR
ELKGFRVLHGTEMDILGDGALDFPDEVLKGLDIVVASIHSGFNNSKEVMTSRIVAAMRNPYISIIGHPTGRILGEREGYQ
IDMDEVLRVAKETGTALEINAYPMRLDLEDKHVRRAKELGIMIAVNTDTHAKLQFDFLPYGISVAQRGWLEPADVLNTLD
PDQLLKKLREKRKRMARK

Sequences:

>Translated_578_residues
MKNREIARIFAEIADILEIKEGNVFKIRAYRRAALNLDGFSRDLAQLSHKELLEIPGVGADLAGRIEEYLQTGTMAAYEE
LKQEVPAGVFALLAIPDLGPKTAKAIYDALQIASIEELEKAALEHKLIGIKGIKQKTEENILKGIAAVKRGRERQPLGRM
LPAAQDLVEALKERAPVERIEVAGSIRRRRDSIKDIDIVATSPDPAAVMAAFVDLPQVHDVIMRGPTRASVTIREGVQVD
LRVVDPASYGAALAYLTGSQAHNVRLREMAQKRGLKINEYGIFREEDNQRLGGVDEEDVYRLLDLAFVPPVLREDRGEIE
AALLGKLPQLVTQADIRGDLHVHSRWSDGAHGIAELVEAARERGLSYIAVTDHSQGLGVARGLTVERLREQAAEIKELNR
ELKGFRVLHGTEMDILGDGALDFPDEVLKGLDIVVASIHSGFNNSKEVMTSRIVAAMRNPYISIIGHPTGRILGEREGYQ
IDMDEVLRVAKETGTALEINAYPMRLDLEDKHVRRAKELGIMIAVNTDTHAKLQFDFLPYGISVAQRGWLEPADVLNTLD
PDQLLKKLREKRKRMARK
>Mature_578_residues
MKNREIARIFAEIADILEIKEGNVFKIRAYRRAALNLDGFSRDLAQLSHKELLEIPGVGADLAGRIEEYLQTGTMAAYEE
LKQEVPAGVFALLAIPDLGPKTAKAIYDALQIASIEELEKAALEHKLIGIKGIKQKTEENILKGIAAVKRGRERQPLGRM
LPAAQDLVEALKERAPVERIEVAGSIRRRRDSIKDIDIVATSPDPAAVMAAFVDLPQVHDVIMRGPTRASVTIREGVQVD
LRVVDPASYGAALAYLTGSQAHNVRLREMAQKRGLKINEYGIFREEDNQRLGGVDEEDVYRLLDLAFVPPVLREDRGEIE
AALLGKLPQLVTQADIRGDLHVHSRWSDGAHGIAELVEAARERGLSYIAVTDHSQGLGVARGLTVERLREQAAEIKELNR
ELKGFRVLHGTEMDILGDGALDFPDEVLKGLDIVVASIHSGFNNSKEVMTSRIVAAMRNPYISIIGHPTGRILGEREGYQ
IDMDEVLRVAKETGTALEINAYPMRLDLEDKHVRRAKELGIMIAVNTDTHAKLQFDFLPYGISVAQRGWLEPADVLNTLD
PDQLLKKLREKRKRMARK

Specific function: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a sing

COG id: COG1796

COG function: function code L; DNA polymerase IV (family X)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PHP family [H]

Homologues:

Organism=Homo sapiens, GI4505931, Length=303, Percent_Identity=26.4026402640264, Blast_Score=80, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002054
- InterPro:   IPR010996
- InterPro:   IPR003583
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR022311 [H]

Pfam domain/function: PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 64196; Mature: 64196

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNREIARIFAEIADILEIKEGNVFKIRAYRRAALNLDGFSRDLAQLSHKELLEIPGVGA
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCH
DLAGRIEEYLQTGTMAAYEELKQEVPAGVFALLAIPDLGPKTAKAIYDALQIASIEELEK
HHHHHHHHHHHHCHHHHHHHHHHHCCHHHEEEHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AALEHKLIGIKGIKQKTEENILKGIAAVKRGRERQPLGRMLPAAQDLVEALKERAPVERI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHCCCHHHH
EVAGSIRRRRDSIKDIDIVATSPDPAAVMAAFVDLPQVHDVIMRGPTRASVTIREGVQVD
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCEEE
LRVVDPASYGAALAYLTGSQAHNVRLREMAQKRGLKINEYGIFREEDNQRLGGVDEEDVY
EEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECCCCEECCCCCCCCCCCHHHHH
RLLDLAFVPPVLREDRGEIEAALLGKLPQLVTQADIRGDLHVHSRWSDGAHGIAELVEAA
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
RERGLSYIAVTDHSQGLGVARGLTVERLREQAAEIKELNRELKGFRVLHGTEMDILGDGA
HHCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCC
LDFPDEVLKGLDIVVASIHSGFNNSKEVMTSRIVAAMRNPYISIIGHPTGRILGEREGYQ
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCE
IDMDEVLRVAKETGTALEINAYPMRLDLEDKHVRRAKELGIMIAVNTDTHAKLQFDFLPY
ECHHHHHHHHHHCCCEEEEECEEEEECCCHHHHHHHHHCCEEEEEECCCCCEEEEEEECC
GISVAQRGWLEPADVLNTLDPDQLLKKLREKRKRMARK
CHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNREIARIFAEIADILEIKEGNVFKIRAYRRAALNLDGFSRDLAQLSHKELLEIPGVGA
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCH
DLAGRIEEYLQTGTMAAYEELKQEVPAGVFALLAIPDLGPKTAKAIYDALQIASIEELEK
HHHHHHHHHHHHCHHHHHHHHHHHCCHHHEEEHCCCCCCHHHHHHHHHHHHHHHHHHHHH
AALEHKLIGIKGIKQKTEENILKGIAAVKRGRERQPLGRMLPAAQDLVEALKERAPVERI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHCCCHHHH
EVAGSIRRRRDSIKDIDIVATSPDPAAVMAAFVDLPQVHDVIMRGPTRASVTIREGVQVD
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCEEE
LRVVDPASYGAALAYLTGSQAHNVRLREMAQKRGLKINEYGIFREEDNQRLGGVDEEDVY
EEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECCCCEECCCCCCCCCCCHHHHH
RLLDLAFVPPVLREDRGEIEAALLGKLPQLVTQADIRGDLHVHSRWSDGAHGIAELVEAA
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
RERGLSYIAVTDHSQGLGVARGLTVERLREQAAEIKELNRELKGFRVLHGTEMDILGDGA
HHCCCEEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCEEEECCCCEEEECCCC
LDFPDEVLKGLDIVVASIHSGFNNSKEVMTSRIVAAMRNPYISIIGHPTGRILGEREGYQ
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCCCCE
IDMDEVLRVAKETGTALEINAYPMRLDLEDKHVRRAKELGIMIAVNTDTHAKLQFDFLPY
ECHHHHHHHHHHCCCEEEEECEEEEECCCHHHHHHHHHCCEEEEEECCCCCEEEEEEECC
GISVAQRGWLEPADVLNTLDPDQLLKKLREKRKRMARK
CHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]