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The map label for this gene is groEL [H]

Identifier: 197116649

GI number: 197116649

Start: 291508

End: 293151

Strand: Direct

Name: groEL [H]

Synonym: Gbem_0249

Alternate gene names: 197116649

Gene position: 291508-293151 (Clockwise)

Preceding gene: 197116648

Following gene: 197116650

Centisome position: 6.32

GC content: 62.17

Gene sequence:

>1644_bases
ATGGCAGCAAAGATCATCAAGTTCGACCAGGAAGCACGCAACTGCATCCTCAAAGGTGTCAACACCCTGGCAGATGCCGT
TAAAGTGACCCTGGGCCCCAAAGGGCGCAACGTCGTCATCGAGAAATCCTACGGCGCGCCGCTCATCACCAAGGACGGCG
TCACCGTCGCCAAGGAAATCGAGCTGGAAGACAAGTTCGAGAACATGGGCGCGCAGCTGGTGAAGGAAGTCGCTTCCAAG
ACCTCCGACGTAGCCGGCGACGGCACCACCACCGCAACCGTGCTGGCACAGGCGATCTACCGCCAGGGCGCCAAGCTGGT
CGCGGCAGGTCACAACCCGATGGAAATCAAGCGCGGTCTGGATCAGGCTGTCGAGGCCCTGGTCGCCGAGCTGAAGAACA
TCTCCAAGCCGATCAAGGACCACAAGGAAATCGCGCAGGTCGGCACCATCTCCGCCAACAACGACAAGACCATCGGCGAC
ATCATCGCCGAGGCGATGGAGAAGGTCGGCAAGGAAGGGGTCATCACCGTCGAGGAAGCCAAGGCGATGGAAACCACCCT
TGAGACCGTCGAGGGTATGCAGTTCGACCGCGGCTACCTCTCCCCCTACTTCGTGACCGATCCGGAGCGCATGGAAGCCG
CGATGGACAACGTCGCCATCCTGATCCACGACAAGAAGATCGCCAACATGAAGGACCTCCTCCCGGTTCTCGAGCAGACC
GCCAAGTCCGGCCGTCCGCTGCTGATCATCGCCGAGGACATCGAAGGCGAAGCCCTGGCAACCCTCGTGGTCAACAAGCT
GCGCGGCGTCCTTAACGTCTGCGCCGTCAAGGCCCCGGGCTTCGGCGACCGCCGCAAGGCGATGCTCGAAGACATCGCCA
TCCTGACCGGCGGCAAGGTGATCTCCGAGGAAGTCGGCTTCAAACTCGAGAACACCACCCTCGACATGCTGGGCCAGGCG
AAGAAGATCACCGTCGACAAGGACAACACCACCATCATCGACGGCTACGGCGCCGAGGCCGACATCCAGGGCCGCGTCAA
GATGATCCGCGCCCAGATCGATGAGACCTCCTCCGACTACGACCGCGAGAAGCTCCAGGAGCGCCTGGCGAAACTGGTCG
GCGGCGTTGCCGTCATCAAGGTCGGTGCTGCCACCGAGATCGAGATGAAGGAGAAGAAGGCACGCGTCGAAGACGCACTG
CACGCAACCCGCGCAGCGGTCGACGAGGGGATCGTCCCCGGAGGCGGCGTGGCTTACCTGCGCGCCCTGAAGGTGCTGGA
AAAACTTCAGCTCGCACCGGAGCAGCAGTTCGGGGTCAACGTGATCAAGCGCGCCCTCGAAGAGCCGATCCGTCAGATCT
CCCAGAACGCCGGCGTCGACGGCTCCATTGTCGTGGACAAGGTCAAAAACGGCAAGGATGCCTTCGGCTACAACGCCGCC
GACGACGTCTATGTCGACATGATCGAGGCCGGCATCATCGACCCGACCAAGGTCTCCAGGAGCGCGCTGCAGAACGCCGC
TTCCGTGGCAGGTCTCATGATGACCACCGAAGCAATGATCGCAGACAAACCGAAGGAAGAAGGCGCGATGCCGGCAATGC
CGGGTGGCATGGGCGGCATGGGTGGCATGGGCGGCATGATGTAG

Upstream 100 bases:

>100_bases
GACGACATCCTCGGCGTCGTCGAGAAGTAATCACCGCCTCCCGGTCCGCCGGGGGAAGCAGTTTCGCCACCATTCATTAA
TTCCAAGGAGGATATACGTT

Downstream 100 bases:

>100_bases
TCCTGCCCCCGAAACCCGCAGCAAAAATGAAGGGAGCCGGCCCCAGTGCCGGCTCCCTTTTTTATCGGCAGCTTGCTTCT
CCTGGTTCCCAAGTGGTCGC

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60 [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAKIIKFDQEARNCILKGVNTLADAVKVTLGPKGRNVVIEKSYGAPLITKDGVTVAKEIELEDKFENMGAQLVKEVASK
TSDVAGDGTTTATVLAQAIYRQGAKLVAAGHNPMEIKRGLDQAVEALVAELKNISKPIKDHKEIAQVGTISANNDKTIGD
IIAEAMEKVGKEGVITVEEAKAMETTLETVEGMQFDRGYLSPYFVTDPERMEAAMDNVAILIHDKKIANMKDLLPVLEQT
AKSGRPLLIIAEDIEGEALATLVVNKLRGVLNVCAVKAPGFGDRRKAMLEDIAILTGGKVISEEVGFKLENTTLDMLGQA
KKITVDKDNTTIIDGYGAEADIQGRVKMIRAQIDETSSDYDREKLQERLAKLVGGVAVIKVGAATEIEMKEKKARVEDAL
HATRAAVDEGIVPGGGVAYLRALKVLEKLQLAPEQQFGVNVIKRALEEPIRQISQNAGVDGSIVVDKVKNGKDAFGYNAA
DDVYVDMIEAGIIDPTKVSRSALQNAASVAGLMMTTEAMIADKPKEEGAMPAMPGGMGGMGGMGGMM

Sequences:

>Translated_547_residues
MAAKIIKFDQEARNCILKGVNTLADAVKVTLGPKGRNVVIEKSYGAPLITKDGVTVAKEIELEDKFENMGAQLVKEVASK
TSDVAGDGTTTATVLAQAIYRQGAKLVAAGHNPMEIKRGLDQAVEALVAELKNISKPIKDHKEIAQVGTISANNDKTIGD
IIAEAMEKVGKEGVITVEEAKAMETTLETVEGMQFDRGYLSPYFVTDPERMEAAMDNVAILIHDKKIANMKDLLPVLEQT
AKSGRPLLIIAEDIEGEALATLVVNKLRGVLNVCAVKAPGFGDRRKAMLEDIAILTGGKVISEEVGFKLENTTLDMLGQA
KKITVDKDNTTIIDGYGAEADIQGRVKMIRAQIDETSSDYDREKLQERLAKLVGGVAVIKVGAATEIEMKEKKARVEDAL
HATRAAVDEGIVPGGGVAYLRALKVLEKLQLAPEQQFGVNVIKRALEEPIRQISQNAGVDGSIVVDKVKNGKDAFGYNAA
DDVYVDMIEAGIIDPTKVSRSALQNAASVAGLMMTTEAMIADKPKEEGAMPAMPGGMGGMGGMGGMM
>Mature_546_residues
AAKIIKFDQEARNCILKGVNTLADAVKVTLGPKGRNVVIEKSYGAPLITKDGVTVAKEIELEDKFENMGAQLVKEVASKT
SDVAGDGTTTATVLAQAIYRQGAKLVAAGHNPMEIKRGLDQAVEALVAELKNISKPIKDHKEIAQVGTISANNDKTIGDI
IAEAMEKVGKEGVITVEEAKAMETTLETVEGMQFDRGYLSPYFVTDPERMEAAMDNVAILIHDKKIANMKDLLPVLEQTA
KSGRPLLIIAEDIEGEALATLVVNKLRGVLNVCAVKAPGFGDRRKAMLEDIAILTGGKVISEEVGFKLENTTLDMLGQAK
KITVDKDNTTIIDGYGAEADIQGRVKMIRAQIDETSSDYDREKLQERLAKLVGGVAVIKVGAATEIEMKEKKARVEDALH
ATRAAVDEGIVPGGGVAYLRALKVLEKLQLAPEQQFGVNVIKRALEEPIRQISQNAGVDGSIVVDKVKNGKDAFGYNAAD
DVYVDMIEAGIIDPTKVSRSALQNAASVAGLMMTTEAMIADKPKEEGAMPAMPGGMGGMGGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family [H]

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=51.8026565464896, Blast_Score=527, Evalue=1e-149,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=51.8026565464896, Blast_Score=527, Evalue=1e-149,
Organism=Homo sapiens, GI5453607, Length=539, Percent_Identity=23.5621521335807, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI38455427, Length=528, Percent_Identity=22.9166666666667, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI261399877, Length=509, Percent_Identity=23.1827111984283, Blast_Score=66, Evalue=8e-11,
Organism=Escherichia coli, GI1790586, Length=530, Percent_Identity=67.3584905660377, Blast_Score=699, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17555558, Length=528, Percent_Identity=52.4621212121212, Blast_Score=545, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI193210679, Length=210, Percent_Identity=52.8571428571429, Blast_Score=220, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323288, Length=526, Percent_Identity=54.1825095057034, Blast_Score=551, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322350, Length=557, Percent_Identity=23.5188509874327, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322524, Length=180, Percent_Identity=26.1111111111111, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24641193, Length=527, Percent_Identity=51.8026565464896, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24641191, Length=527, Percent_Identity=51.8026565464896, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45550936, Length=527, Percent_Identity=50.4743833017078, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550132, Length=527, Percent_Identity=50.4743833017078, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45550935, Length=527, Percent_Identity=50.4743833017078, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI17864606, Length=554, Percent_Identity=44.2238267148014, Blast_Score=462, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24584129, Length=535, Percent_Identity=37.5700934579439, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI19921262, Length=535, Percent_Identity=37.5700934579439, Blast_Score=342, Evalue=4e-94,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58387; Mature: 58256

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKIIKFDQEARNCILKGVNTLADAVKVTLGPKGRNVVIEKSYGAPLITKDGVTVAKEI
CCCCCEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCEEECCCCEEEHHC
ELEDKFENMGAQLVKEVASKTSDVAGDGTTTATVLAQAIYRQGAKLVAAGHNPMEIKRGL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
DQAVEALVAELKNISKPIKDHKEIAQVGTISANNDKTIGDIIAEAMEKVGKEGVITVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHH
KAMETTLETVEGMQFDRGYLSPYFVTDPERMEAAMDNVAILIHDKKIANMKDLLPVLEQT
HHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHCCEEEEEECCHHCCHHHHHHHHHHH
AKSGRPLLIIAEDIEGEALATLVVNKLRGVLNVCAVKAPGFGDRRKAMLEDIAILTGGKV
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCH
ISEEVGFKLENTTLDMLGQAKKITVDKDNTTIIDGYGAEADIQGRVKMIRAQIDETSSDY
HHHHHCCEECCCHHHHCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
DREKLQERLAKLVGGVAVIKVGAATEIEMKEKKARVEDALHATRAAVDEGIVPGGGVAYL
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
RALKVLEKLQLAPEQQFGVNVIKRALEEPIRQISQNAGVDGSIVVDKVKNGKDAFGYNAA
HHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
DDVYVDMIEAGIIDPTKVSRSALQNAASVAGLMMTTEAMIADKPKEEGAMPAMPGGMGGM
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
GGMGGMM
CCCCCCC
>Mature Secondary Structure 
AAKIIKFDQEARNCILKGVNTLADAVKVTLGPKGRNVVIEKSYGAPLITKDGVTVAKEI
CCCCEEHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCEEECCCCEEEHHC
ELEDKFENMGAQLVKEVASKTSDVAGDGTTTATVLAQAIYRQGAKLVAAGHNPMEIKRGL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
DQAVEALVAELKNISKPIKDHKEIAQVGTISANNDKTIGDIIAEAMEKVGKEGVITVEEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHH
KAMETTLETVEGMQFDRGYLSPYFVTDPERMEAAMDNVAILIHDKKIANMKDLLPVLEQT
HHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHCCEEEEEECCHHCCHHHHHHHHHHH
AKSGRPLLIIAEDIEGEALATLVVNKLRGVLNVCAVKAPGFGDRRKAMLEDIAILTGGKV
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCH
ISEEVGFKLENTTLDMLGQAKKITVDKDNTTIIDGYGAEADIQGRVKMIRAQIDETSSDY
HHHHHCCEECCCHHHHCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
DREKLQERLAKLVGGVAVIKVGAATEIEMKEKKARVEDALHATRAAVDEGIVPGGGVAYL
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
RALKVLEKLQLAPEQQFGVNVIKRALEEPIRQISQNAGVDGSIVVDKVKNGKDAFGYNAA
HHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
DDVYVDMIEAGIIDPTKVSRSALQNAASVAGLMMTTEAMIADKPKEEGAMPAMPGGMGGM
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCC
GGMGGMM
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA