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The map label for this gene is nuoE-2 [H]

Identifier: 197116577

GI number: 197116577

Start: 207254

End: 207724

Strand: Reverse

Name: nuoE-2 [H]

Synonym: Gbem_0177

Alternate gene names: 197116577

Gene position: 207724-207254 (Counterclockwise)

Preceding gene: 197116578

Following gene: 197116576

Centisome position: 4.5

GC content: 64.54

Gene sequence:

>471_bases
ATGATATCGGAAGCACTGAAAAAATCGCTGACCGAACGGGTGGCAGGCGCCATCACCCCGCGCGAGGCTGCGGTCGACGT
GATGAAGGAACTGCAGGCGCACTACGGCTGGCTCACCGACGAGGCCGTCGCCGAGGCTGCCTCTCTCCTGGGGCTGTCGC
CGCTGCAGGTGGAGGAACTGGCCACCTTTTACGAGATGATCTACCGCCGGCCGGTGGGTAAGAAGGTGATCCACGTCTGC
GACTCCATCTCCTGCTGGTGCGCGGACTGCGACGGCATCATCGAGCACCTGAAAAAGCGGCTGGGGGTGGAGCTTGGGGG
GACTACGGCGGACGGGATGTACACCCTTCTTCCCTGCGCCTGCATGGGAAGGTGCGGCGACAGCCCCGCGATGTCGGTGG
GAGGGACCCCCTACGGGCACCTGACGCCGCATCTCGTGGACGAGATCCTGGAAAAGGAACGGCGGGCGTAA

Upstream 100 bases:

>100_bases
AGCCGCGGCTGGCTGGTGTCCGATTTCCTGGCCATTCTTGGGTCGTTGGATTTCGTGCTTTCGGATTTGGACCGGTAATA
AACGAGGAGCGGAAACGCTG

Downstream 100 bases:

>100_bases
AAGGACGACATGGATCTCCCTCTCTTTCGACATAACCGCCGGGACCGCTCGGTCGCCTTCAAGGAGTACCGCTCCGAAGG
GGGCTTCGAGGCGCTCAAGA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MISEALKKSLTERVAGAITPREAAVDVMKELQAHYGWLTDEAVAEAASLLGLSPLQVEELATFYEMIYRRPVGKKVIHVC
DSISCWCADCDGIIEHLKKRLGVELGGTTADGMYTLLPCACMGRCGDSPAMSVGGTPYGHLTPHLVDEILEKERRA

Sequences:

>Translated_156_residues
MISEALKKSLTERVAGAITPREAAVDVMKELQAHYGWLTDEAVAEAASLLGLSPLQVEELATFYEMIYRRPVGKKVIHVC
DSISCWCADCDGIIEHLKKRLGVELGGTTADGMYTLLPCACMGRCGDSPAMSVGGTPYGHLTPHLVDEILEKERRA
>Mature_156_residues
MISEALKKSLTERVAGAITPREAAVDVMKELQAHYGWLTDEAVAEAASLLGLSPLQVEELATFYEMIYRRPVGKKVIHVC
DSISCWCADCDGIIEHLKKRLGVELGGTTADGMYTLLPCACMGRCGDSPAMSVGGTPYGHLTPHLVDEILEKERRA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=130, Percent_Identity=36.1538461538462, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1788621, Length=151, Percent_Identity=37.7483443708609, Blast_Score=119, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17561328, Length=137, Percent_Identity=35.7664233576642, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI18859877, Length=131, Percent_Identity=36.6412213740458, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21355353, Length=124, Percent_Identity=36.2903225806452, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 16950; Mature: 16950

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISEALKKSLTERVAGAITPREAAVDVMKELQAHYGWLTDEAVAEAASLLGLSPLQVEEL
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
ATFYEMIYRRPVGKKVIHVCDSISCWCADCDGIIEHLKKRLGVELGGTTADGMYTLLPCA
HHHHHHHHHCCCHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CMGRCGDSPAMSVGGTPYGHLTPHLVDEILEKERRA
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MISEALKKSLTERVAGAITPREAAVDVMKELQAHYGWLTDEAVAEAASLLGLSPLQVEEL
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHH
ATFYEMIYRRPVGKKVIHVCDSISCWCADCDGIIEHLKKRLGVELGGTTADGMYTLLPCA
HHHHHHHHHCCCHHHHHHHHCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
CMGRCGDSPAMSVGGTPYGHLTPHLVDEILEKERRA
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]