Click here to switch to the map view.

The map label for this gene is nuoF-2 [H]

Identifier: 197116576

GI number: 197116576

Start: 205979

End: 207244

Strand: Reverse

Name: nuoF-2 [H]

Synonym: Gbem_0176

Alternate gene names: 197116576

Gene position: 207244-205979 (Counterclockwise)

Preceding gene: 197116577

Following gene: 197116575

Centisome position: 4.49

GC content: 65.24

Gene sequence:

>1266_bases
ATGGATCTCCCTCTCTTTCGACATAACCGCCGGGACCGCTCGGTCGCCTTCAAGGAGTACCGCTCCGAAGGGGGCTTCGA
GGCGCTCAAGAGGGCGATCACGGGGATGACGCCGGCCGAGGTGCAGCAGACCGTCATCGACTCCGGCCTGCGCGGGCGGG
GTGGCGCCGGCTTTCCGACCGGGAAGAAGTGGTCCTTCGTGCCGCGGGACCTCCCCGGGCCGCGCTGGATCATCTGCAAC
TGCGACGAGATGGAACCGGGGACCTACAAGGACCGGGTGCTCCTGGAGCATAACCCCTACAGCCTGATCGAGGGGATGAC
GCTCGCCTGTTACGCGCTAGGAGTGAGGCACGGCTTCATCTTCATCCGCAAGGGGTACGAGCAGGCCGCCGAGAATCTGG
CCCGCGCCATCGACGAGGCAAAAAAGGAAGGGTTTCTGGGGGAGCGCATCCTCGGGAGCGAGCACTCCATCGACATCGAC
CTGCATCTTTCCGCCGGCCGCTACATCTGCGGCGAGGAGACGGCGCTCATCAACGCCCTGGAGGGGAAGCGCCCGAACCC
GCGGCAAAAGCCCCCCTTCCCGGCGGTGAAGGGGCTTTGGGCCGGGCCTACGGTGGTGAACAACGTCGAGACCCTGGCCA
ACATACCGGGGATCCTCGCTCGCGGCGCCGCCTGGTTCAAGGGGCTGGCGATGAACCCAGAGGGGGCGGGCAGCAAGCTC
TTCTGCGTGAGCGGATCGGTGAAGGACCGCGCCTGCGTCGAGCTTCCCATCGGCATACCGCTAGGCGACATCATCGACGG
CCCCTGCGGCGGCATGCGCCTGGGAAAGAGGTTCAAGGCCTGCATCCCGGGGGGCGCCTCGACGCAGTTTCTCACCGCGG
AGCACCTGAAGGTCCCCATGGATTACGACCCCGTCGCCAAGGTCGGCTCCCGGCTCGGCTCCGGCGGCATCATCGTCTTC
GACCAGGACCACTGTCTGGTGGAGGCGACCCTGAACCTGATCACCTTTTTCGCCCGCGAATCGTGCGGCTGGTGTACCCC
GTGCCGCGAGGGGCTGCCCTACGTGAGGGAGATTCTGACCAGGATCGAGACCGGGTACGGCAAGGAAGACGACATCGCCA
TCCTGCGCGAGCACATAAAGTACCTGAATTACTCGTTCTGCGCCCTGGCCCCCGGCGCCATGGCGCCGCTGGACGGGCTT
TTGCGGCACTTCGAGGACGAGGTGCGGGAGCATATCGTGAAGAAGAAATGTCCCTTGAAGAAATAG

Upstream 100 bases:

>100_bases
CAGCCCCGCGATGTCGGTGGGAGGGACCCCCTACGGGCACCTGACGCCGCATCTCGTGGACGAGATCCTGGAAAAGGAAC
GGCGGGCGTAAAAGGACGAC

Downstream 100 bases:

>100_bases
GGCGAAAGGACCGTAGAGCTATCAGATGCCGAAGCTGATCATAGATGACATACCGGTGGAGGTCCCGCCGGGAACCAGCG
TGCTGGAAGCCGCCCGCAGC

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F; NUO6 [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDLPLFRHNRRDRSVAFKEYRSEGGFEALKRAITGMTPAEVQQTVIDSGLRGRGGAGFPTGKKWSFVPRDLPGPRWIICN
CDEMEPGTYKDRVLLEHNPYSLIEGMTLACYALGVRHGFIFIRKGYEQAAENLARAIDEAKKEGFLGERILGSEHSIDID
LHLSAGRYICGEETALINALEGKRPNPRQKPPFPAVKGLWAGPTVVNNVETLANIPGILARGAAWFKGLAMNPEGAGSKL
FCVSGSVKDRACVELPIGIPLGDIIDGPCGGMRLGKRFKACIPGGASTQFLTAEHLKVPMDYDPVAKVGSRLGSGGIIVF
DQDHCLVEATLNLITFFARESCGWCTPCREGLPYVREILTRIETGYGKEDDIAILREHIKYLNYSFCALAPGAMAPLDGL
LRHFEDEVREHIVKKKCPLKK

Sequences:

>Translated_421_residues
MDLPLFRHNRRDRSVAFKEYRSEGGFEALKRAITGMTPAEVQQTVIDSGLRGRGGAGFPTGKKWSFVPRDLPGPRWIICN
CDEMEPGTYKDRVLLEHNPYSLIEGMTLACYALGVRHGFIFIRKGYEQAAENLARAIDEAKKEGFLGERILGSEHSIDID
LHLSAGRYICGEETALINALEGKRPNPRQKPPFPAVKGLWAGPTVVNNVETLANIPGILARGAAWFKGLAMNPEGAGSKL
FCVSGSVKDRACVELPIGIPLGDIIDGPCGGMRLGKRFKACIPGGASTQFLTAEHLKVPMDYDPVAKVGSRLGSGGIIVF
DQDHCLVEATLNLITFFARESCGWCTPCREGLPYVREILTRIETGYGKEDDIAILREHIKYLNYSFCALAPGAMAPLDGL
LRHFEDEVREHIVKKKCPLKK
>Mature_421_residues
MDLPLFRHNRRDRSVAFKEYRSEGGFEALKRAITGMTPAEVQQTVIDSGLRGRGGAGFPTGKKWSFVPRDLPGPRWIICN
CDEMEPGTYKDRVLLEHNPYSLIEGMTLACYALGVRHGFIFIRKGYEQAAENLARAIDEAKKEGFLGERILGSEHSIDID
LHLSAGRYICGEETALINALEGKRPNPRQKPPFPAVKGLWAGPTVVNNVETLANIPGILARGAAWFKGLAMNPEGAGSKL
FCVSGSVKDRACVELPIGIPLGDIIDGPCGGMRLGKRFKACIPGGASTQFLTAEHLKVPMDYDPVAKVGSRLGSGGIIVF
DQDHCLVEATLNLITFFARESCGWCTPCREGLPYVREILTRIETGYGKEDDIAILREHIKYLNYSFCALAPGAMAPLDGL
LRHFEDEVREHIVKKKCPLKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=381, Percent_Identity=41.994750656168, Blast_Score=289, Evalue=4e-78,
Organism=Homo sapiens, GI260656005, Length=381, Percent_Identity=41.994750656168, Blast_Score=288, Evalue=5e-78,
Organism=Escherichia coli, GI1788620, Length=413, Percent_Identity=49.1525423728814, Blast_Score=404, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17531727, Length=371, Percent_Identity=42.5876010781671, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI19920794, Length=369, Percent_Identity=42.8184281842818, Blast_Score=294, Evalue=7e-80,
Organism=Drosophila melanogaster, GI45550452, Length=379, Percent_Identity=43.5356200527704, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24653819, Length=384, Percent_Identity=38.28125, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24653817, Length=360, Percent_Identity=36.9444444444444, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46283; Mature: 46283

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPLFRHNRRDRSVAFKEYRSEGGFEALKRAITGMTPAEVQQTVIDSGLRGRGGAGFPT
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
GKKWSFVPRDLPGPRWIICNCDEMEPGTYKDRVLLEHNPYSLIEGMTLACYALGVRHGFI
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCEE
FIRKGYEQAAENLARAIDEAKKEGFLGERILGSEHSIDIDLHLSAGRYICGEETALINAL
EEECCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEECCCCEECCCHHHHHHHH
EGKRPNPRQKPPFPAVKGLWAGPTVVNNVETLANIPGILARGAAWFKGLAMNPEGAGSKL
CCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEE
FCVSGSVKDRACVELPIGIPLGDIIDGPCGGMRLGKRFKACIPGGASTQFLTAEHLKVPM
EEECCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEHHHEECCC
DYDPVAKVGSRLGSGGIIVFDQDHCLVEATLNLITFFARESCGWCTPCREGLPYVREILT
CCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHH
RIETGYGKEDDIAILREHIKYLNYSFCALAPGAMAPLDGLLRHFEDEVREHIVKKKCPLK
HHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
K
C
>Mature Secondary Structure
MDLPLFRHNRRDRSVAFKEYRSEGGFEALKRAITGMTPAEVQQTVIDSGLRGRGGAGFPT
CCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
GKKWSFVPRDLPGPRWIICNCDEMEPGTYKDRVLLEHNPYSLIEGMTLACYALGVRHGFI
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCEE
FIRKGYEQAAENLARAIDEAKKEGFLGERILGSEHSIDIDLHLSAGRYICGEETALINAL
EEECCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEECCCCEECCCHHHHHHHH
EGKRPNPRQKPPFPAVKGLWAGPTVVNNVETLANIPGILARGAAWFKGLAMNPEGAGSKL
CCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCEE
FCVSGSVKDRACVELPIGIPLGDIIDGPCGGMRLGKRFKACIPGGASTQFLTAEHLKVPM
EEECCCCCCCEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEHHHEECCC
DYDPVAKVGSRLGSGGIIVFDQDHCLVEATLNLITFFARESCGWCTPCREGLPYVREILT
CCCHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHH
RIETGYGKEDDIAILREHIKYLNYSFCALAPGAMAPLDGLLRHFEDEVREHIVKKKCPLK
HHHHCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7690854; 8157582; 9205837; 9278503; 8366049 [H]