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The map label for this gene is nuoI-2 [H]

Identifier: 197116572

GI number: 197116572

Start: 201864

End: 202394

Strand: Reverse

Name: nuoI-2 [H]

Synonym: Gbem_0172

Alternate gene names: 197116572

Gene position: 202394-201864 (Counterclockwise)

Preceding gene: 197116573

Following gene: 197116571

Centisome position: 4.39

GC content: 65.54

Gene sequence:

>531_bases
ATGCCCATTCTGACCGACATAAAAGAGATCTTGACCGGCCTTTCCATCACCTTCAGCCATATCTGGCGGAAGCCCGTAAC
CGTGCAGTTCCCCGAGGAGCAAAGGCCGATGCCCCCCCGTTTCCGCGGCAGCATCGTGCTCACCCGGGACCCCGACGGCG
CCGAGCGCTGCGTCGCCTGCTACCTCTGCTCCGGCGCCTGCCCCGTGGACTGCATCTCCATGGCCGCTGCCGAAGGGGAG
AACGGCCGCCGCTACGCCGCCTGGTTCCGCATCAACTTCTCCCGCTGCATCCTGTGCGGCATGTGCGCCGAGGCGTGCCC
GACCCTTGCCATCCAGATGTCGCCCGAGATGTTCCGCTGCAAGCGGCAGGTCATGGACATGGTGTACGAGAAGGAGGACC
TCCTCATCGACGGCACCGGGAAGGATCCCGAATACAACTATTACCGGCATGCGGGAATCGGCGTGGTGCAGCCGCGGGGG
AGCTCCCCCAGCGAGCAGGAACCTGCCGACCCGCGCAGCCTGCTGCCGTAA

Upstream 100 bases:

>100_bases
CTGTCTGCCGGGCCTCCGGCCCTCCCGGCAGATGCCAGGTTCCTCCGGGGCGGCTGAAATCGATCTCCCCAAGCGGCGCC
CGTGCCGCAAACAGGAAAAG

Downstream 100 bases:

>100_bases
GAGAAATACCACCGGAGCGAAATGGAACGGACCATCTTCTACATACTTGCAGCCGTCACCGTGATAGGGACGCTCTGCGC
CATCACGGCGCGGCAGGCGG

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 1; NDH-1 subunit I 1 [H]

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MPILTDIKEILTGLSITFSHIWRKPVTVQFPEEQRPMPPRFRGSIVLTRDPDGAERCVACYLCSGACPVDCISMAAAEGE
NGRRYAAWFRINFSRCILCGMCAEACPTLAIQMSPEMFRCKRQVMDMVYEKEDLLIDGTGKDPEYNYYRHAGIGVVQPRG
SSPSEQEPADPRSLLP

Sequences:

>Translated_176_residues
MPILTDIKEILTGLSITFSHIWRKPVTVQFPEEQRPMPPRFRGSIVLTRDPDGAERCVACYLCSGACPVDCISMAAAEGE
NGRRYAAWFRINFSRCILCGMCAEACPTLAIQMSPEMFRCKRQVMDMVYEKEDLLIDGTGKDPEYNYYRHAGIGVVQPRG
SSPSEQEPADPRSLLP
>Mature_175_residues
PILTDIKEILTGLSITFSHIWRKPVTVQFPEEQRPMPPRFRGSIVLTRDPDGAERCVACYLCSGACPVDCISMAAAEGEN
GRRYAAWFRINFSRCILCGMCAEACPTLAIQMSPEMFRCKRQVMDMVYEKEDLLIDGTGKDPEYNYYRHAGIGVVQPRGS
SPSEQEPADPRSLLP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=136, Percent_Identity=38.9705882352941, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788617, Length=180, Percent_Identity=55.5555555555556, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17555194, Length=138, Percent_Identity=36.231884057971, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864306, Length=136, Percent_Identity=39.7058823529412, Blast_Score=118, Evalue=2e-27,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19740; Mature: 19609

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
10.2 %Cys+Met (Translated Protein)
5.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILTDIKEILTGLSITFSHIWRKPVTVQFPEEQRPMPPRFRGSIVLTRDPDGAERCVAC
CCCHHHHHHHHHCHHHHHHHHHCCCCEEECCHHCCCCCCCCCCCEEEEECCCCHHHHHHH
YLCSGACPVDCISMAAAEGENGRRYAAWFRINFSRCILCGMCAEACPTLAIQMSPEMFRC
HHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHCCEEEEEECHHHHHH
KRQVMDMVYEKEDLLIDGTGKDPEYNYYRHAGIGVVQPRGSSPSEQEPADPRSLLP
HHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PILTDIKEILTGLSITFSHIWRKPVTVQFPEEQRPMPPRFRGSIVLTRDPDGAERCVAC
CCHHHHHHHHHCHHHHHHHHHCCCCEEECCHHCCCCCCCCCCCEEEEECCCCHHHHHHH
YLCSGACPVDCISMAAAEGENGRRYAAWFRINFSRCILCGMCAEACPTLAIQMSPEMFRC
HHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHHHHHHCCEEEEEECHHHHHH
KRQVMDMVYEKEDLLIDGTGKDPEYNYYRHAGIGVVQPRGSSPSEQEPADPRSLLP
HHHHHHHHHCCCCEEEECCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA