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The map label for this gene is nuoN-2

Identifier: 197116566

GI number: 197116566

Start: 195654

End: 197048

Strand: Reverse

Name: nuoN-2

Synonym: Gbem_0166

Alternate gene names: 197116566

Gene position: 197048-195654 (Counterclockwise)

Preceding gene: 197116567

Following gene: 197116565

Centisome position: 4.27

GC content: 64.66

Gene sequence:

>1395_bases
ATGATCATCCCTGACCTACATACCATCATGCCGATCATCATCACCGGGGTGGGGGCGCTGGTCGTGCTCCTTTGCGGGCC
GCTGCTCCTTAGCGGGACCACCAGCTCTATCGCGGCGGTTACTGCGGCGGCAGCGGGGCTTTGGGCAATTTTCACCGCGC
CCTCGGCTGTGCCTGCCGTCGCCGGGCTTACCTTCACGCCGCTGGCCCAGTTCCTGATCCCGCTTTTTTGCGCGGCGGCA
CTCCTGACGCTGCTCCTCTCCCACGGCTACAACGAAAACCGCGCCATCAAGGGAGAGGAATACCCGGCGACGGTCCTCTT
CGCACTGTTCGCTTTGTGCGTGCTCCCCTGCGCCTCGAACCTCCTGATCCTCTTCCTGGCTATCGAGTCGGTCTCCTTCG
CCTTCTACATCCTGGTCACCATGGACCTCCAGCGGGCGGAATCGGGCGAGGCGGGCCTGAAATACCTCCTCATGGGGGCG
GTCGCCGCCGCCTTCACCGCTTTCGGCTTCGCCCTCATCTTCGCCGGCAGCGGTACCCTCGAACTTGCCGGCGCCGTGCC
CCACGCGGATAACCGCGCCATCGTCGCCGCCGGTTGGGGCGTGGTCATCATGGGCATGGCCTTCAAGCTTTCGTTGGTCC
CCGCGCACCTCTGGACTCCTGACGTCTACCAGGGAGCTCCGGCCCCGGTCGCAGCCTTTCTTTCCACCAGTTCCAAGGTG
GCGGCGGCTTCGCTGCTGCTCATGCTGCTCACCTTCTCCCCCCCGATGCCGGAATTGCGCATCCCGCTTGTGCTGCTGTC
GCTCCTCTCGATGGTGGTAGGAAACCTGGCGGCGCTCCGGCAGCAGAACATCAAGAGGATGCTCGCCTACTCCTCCATCG
CCCACATGGGCTACCTCACCCTCGCGCTTCTCACCGGCACCAGGGAGGGATACGCCGCGCTCCTCCTTTACGGCGCTATC
TACACCGCCATGAATCTTGCCGCTTTCGGCGCCATTGCCTCGCTCTCACTGGAAAAGGAAAGGGACCAGATAGCGGACTA
CTCCGGGCTCGGTTTCAGCGCTCCGCTGCGCGGCGGCGTGCTTGCCCTCGCCATGCTGGCACTGGCCGGCCTGCCACCTA
CCGGAGGCTTCATCGGCAAGTTCTTCATCTTCTATTCCGCCATCAAGGGGGGCGAAACGGCGCTCGCCATTGTCGGTATC
ATAAGCGCCGCGGTCTCCTCCTTTTTCTATCTCAAGGTCGTCGCGAGCCTCTACATGCACCGCTCCTCCTCCCCTGCCCC
CCGCGCCGCTGCCGCTGCCGACGCCGTCGCCCTTTGCTGCGCGGCCGCCTTTATCCTGCTCCTGGGGGTATTCCCGGCCC
CGCTGTTACGGTTGGTCGACGCCGCGCTGCAGTAA

Upstream 100 bases:

>100_bases
TTCAATGTCCCTCACCTGAAGGATGGAATTCAACGTTTTGTGCTTTAGGTTTTCCTCTGTGTACCTCTGTGGTGAGTGCT
TTTGTTTCTGGAGAACCAAG

Downstream 100 bases:

>100_bases
GCGCCTGACTCCGTAACCTCTCCAAATTGTCGTGTGACTTTGATTTTAATGAAGAAAGGGGAATAATAATGAAAGAGTTG
AGCTTTGCTGAAGTTGTGGA

Product: NADH dehydrogenase I subunit N lipoprotein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIIPDLHTIMPIIITGVGALVVLLCGPLLLSGTTSSIAAVTAAAAGLWAIFTAPSAVPAVAGLTFTPLAQFLIPLFCAAA
LLTLLLSHGYNENRAIKGEEYPATVLFALFALCVLPCASNLLILFLAIESVSFAFYILVTMDLQRAESGEAGLKYLLMGA
VAAAFTAFGFALIFAGSGTLELAGAVPHADNRAIVAAGWGVVIMGMAFKLSLVPAHLWTPDVYQGAPAPVAAFLSTSSKV
AAASLLLMLLTFSPPMPELRIPLVLLSLLSMVVGNLAALRQQNIKRMLAYSSIAHMGYLTLALLTGTREGYAALLLYGAI
YTAMNLAAFGAIASLSLEKERDQIADYSGLGFSAPLRGGVLALAMLALAGLPPTGGFIGKFFIFYSAIKGGETALAIVGI
ISAAVSSFFYLKVVASLYMHRSSSPAPRAAAAADAVALCCAAAFILLLGVFPAPLLRLVDAALQ

Sequences:

>Translated_464_residues
MIIPDLHTIMPIIITGVGALVVLLCGPLLLSGTTSSIAAVTAAAAGLWAIFTAPSAVPAVAGLTFTPLAQFLIPLFCAAA
LLTLLLSHGYNENRAIKGEEYPATVLFALFALCVLPCASNLLILFLAIESVSFAFYILVTMDLQRAESGEAGLKYLLMGA
VAAAFTAFGFALIFAGSGTLELAGAVPHADNRAIVAAGWGVVIMGMAFKLSLVPAHLWTPDVYQGAPAPVAAFLSTSSKV
AAASLLLMLLTFSPPMPELRIPLVLLSLLSMVVGNLAALRQQNIKRMLAYSSIAHMGYLTLALLTGTREGYAALLLYGAI
YTAMNLAAFGAIASLSLEKERDQIADYSGLGFSAPLRGGVLALAMLALAGLPPTGGFIGKFFIFYSAIKGGETALAIVGI
ISAAVSSFFYLKVVASLYMHRSSSPAPRAAAAADAVALCCAAAFILLLGVFPAPLLRLVDAALQ
>Mature_464_residues
MIIPDLHTIMPIIITGVGALVVLLCGPLLLSGTTSSIAAVTAAAAGLWAIFTAPSAVPAVAGLTFTPLAQFLIPLFCAAA
LLTLLLSHGYNENRAIKGEEYPATVLFALFALCVLPCASNLLILFLAIESVSFAFYILVTMDLQRAESGEAGLKYLLMGA
VAAAFTAFGFALIFAGSGTLELAGAVPHADNRAIVAAGWGVVIMGMAFKLSLVPAHLWTPDVYQGAPAPVAAFLSTSSKV
AAASLLLMLLTFSPPMPELRIPLVLLSLLSMVVGNLAALRQQNIKRMLAYSSIAHMGYLTLALLTGTREGYAALLLYGAI
YTAMNLAAFGAIASLSLEKERDQIADYSGLGFSAPLRGGVLALAMLALAGLPPTGGFIGKFFIFYSAIKGGETALAIVGI
ISAAVSSFFYLKVVASLYMHRSSSPAPRAAAAADAVALCCAAAFILLLGVFPAPLLRLVDAALQ

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=322, Percent_Identity=23.9130434782609, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI145693160, Length=481, Percent_Identity=34.7193347193347, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1788831, Length=377, Percent_Identity=28.6472148541114, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788614, Length=340, Percent_Identity=28.2352941176471, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788829, Length=350, Percent_Identity=26.5714285714286, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788613, Length=218, Percent_Identity=29.3577981651376, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367154, Length=380, Percent_Identity=23.4210526315789, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_GEOBB (B5E960)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002136993.1
- GeneID:   6782988
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0166
- HOGENOM:   HBG747830
- OMA:   FAFYILV
- ProtClustDB:   CLSK829274
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 48018; Mature: 48018

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x236ef144)-; HASH(0x258f6038)-; HASH(0x26103d14)-; HASH(0x266892a8)-; HASH(0x25dd6190)-; HASH(0x25cf675c)-; HASH(0x26748edc)-; HASH(0x25dd3560)-; HASH(0x267490bc)-; HASH(0x22101390)-; HASH(0x25dd08a8)-; HASH(0x2562f178)-; HASH(0x25af33c8)-; HASH(0x248f5514)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPDLHTIMPIIITGVGALVVLLCGPLLLSGTTSSIAAVTAAAAGLWAIFTAPSAVPAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
AGLTFTPLAQFLIPLFCAAALLTLLLSHGYNENRAIKGEEYPATVLFALFALCVLPCASN
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLILFLAIESVSFAFYILVTMDLQRAESGEAGLKYLLMGAVAAAFTAFGFALIFAGSGTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ELAGAVPHADNRAIVAAGWGVVIMGMAFKLSLVPAHLWTPDVYQGAPAPVAAFLSTSSKV
EEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHCCHHH
AAASLLLMLLTFSPPMPELRIPLVLLSLLSMVVGNLAALRQQNIKRMLAYSSIAHMGYLT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLTGTREGYAALLLYGAIYTAMNLAAFGAIASLSLEKERDQIADYSGLGFSAPLRGGV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LALAMLALAGLPPTGGFIGKFFIFYSAIKGGETALAIVGIISAAVSSFFYLKVVASLYMH
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSSSPAPRAAAAADAVALCCAAAFILLLGVFPAPLLRLVDAALQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MIIPDLHTIMPIIITGVGALVVLLCGPLLLSGTTSSIAAVTAAAAGLWAIFTAPSAVPAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHH
AGLTFTPLAQFLIPLFCAAALLTLLLSHGYNENRAIKGEEYPATVLFALFALCVLPCASN
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLILFLAIESVSFAFYILVTMDLQRAESGEAGLKYLLMGAVAAAFTAFGFALIFAGSGTL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ELAGAVPHADNRAIVAAGWGVVIMGMAFKLSLVPAHLWTPDVYQGAPAPVAAFLSTSSKV
EEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHCCHHH
AAASLLLMLLTFSPPMPELRIPLVLLSLLSMVVGNLAALRQQNIKRMLAYSSIAHMGYLT
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALLTGTREGYAALLLYGAIYTAMNLAAFGAIASLSLEKERDQIADYSGLGFSAPLRGGV
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
LALAMLALAGLPPTGGFIGKFFIFYSAIKGGETALAIVGIISAAVSSFFYLKVVASLYMH
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSSSPAPRAAAAADAVALCCAAAFILLLGVFPAPLLRLVDAALQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA