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The map label for this gene is dusC [H]

Identifier: 197116498

GI number: 197116498

Start: 122824

End: 123849

Strand: Reverse

Name: dusC [H]

Synonym: Gbem_0098

Alternate gene names: 197116498

Gene position: 123849-122824 (Counterclockwise)

Preceding gene: 197116499

Following gene: 197116496

Centisome position: 2.68

GC content: 65.2

Gene sequence:

>1026_bases
ATGACCATGGAAAACCCGCGCCACCAAGCCCTCCAGCCGAATTCCGCAACACCTGCGCCAGCACCCTCGAAGCTCCCCTG
GCTTCCCGGGGTGAAGCCGCTCATGCTGGCCCCCATGCAGGGGTTGACCAACCGTGCGCTCCGCTCGCTTTTCACCAAGT
GGGTGCGTCCGGACGTGGTCTGGACCGAGTTCATGCGGGTGAACCCGGTGTCGGCCAAGAAGCGCCTCATGCCGGGCGAC
CTGCGCGAGATCAGTGCCAGCGAGGACGGGATTCCCCTAGTGGTGCAGTTGATCGGGCACGGGCGGGAGGCGCTCGTTTC
CGCGGCGCAAGCCGCGCAGGACGCGGGGGCGGTGCACCTGAACCTGAACATGGGATGCCCCTACGGCCGCATGACCAGCG
GGCTTACCGGCGGCGGTATGCTGAAAAAGCCGGAACTTCTGGAAGAGATCATCCCTGCGCTGCGCGAGGCGATCCACGGC
TCCTTCTCCATCAAGCTCCGCGCGGGGTACGAAGACCCGCAGCAGGTGTTCTCGCTTCTCCCTCTCTTCGAGCGGTCCGG
TGTGGACTTCCTGGTGCTCCATCCGCGCACCGTGCGCCAGGCCTACAACGGCTGGGCCGATCACGAGGTGACGGCGGAGG
TGGTGCGCCAAACCTCCATCCCGGTCATCGCCAACGGAGACATCAGGAGCGCGGCGGGTGGGCAGCGCCTTTTGGAGCAG
ACCGGCGCCGCCGGCCTCATGCTCGGCAGGGGGGGCATCGGCGAGCCCATGCTCTTCGAGCGTTTGCGCGGGCGCGCTGC
AGCCGAACCGGACCTCGCCGAAAGAAAAGCCATGCTCCACCGCTACCTAATCGACCTGCTTCCCCTTTACTGCGCCCTTT
TCTGCGGCGACGCCCAGGTCCTGGGGAAGATCAAAGGCGTGGTCTCCAATGTCGAAGACGCAGAACTTGAGCGCGAACTG
AAAAAGCTTACAAGATCCAAGACCCTGCAGGCCTTCCGGTCGGCGCTAGAAGAACTCGCCCCTTAG

Upstream 100 bases:

>100_bases
AGCTCCTCGAGATCAAGTAGCAAGCAGTTCGCACCATCCCGGGGAGGCGGCCAGCCGCCTCCCCGTTTCACAACAGCTAC
CGAAGCGAAAGCCCGCACAG

Downstream 100 bases:

>100_bases
CCCCAGTCCCCAGTCCCCAGCCCCCAGCCCCTGCCTCCCTGCTACTTTTCCCAGTAATCCTTAGCGAAGAAGAACATGGC
CGCGGCGAAGGCAAGTGCCC

Product: tRNA dihydrouridine synthase-like flavin mononucleotide-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTMENPRHQALQPNSATPAPAPSKLPWLPGVKPLMLAPMQGLTNRALRSLFTKWVRPDVVWTEFMRVNPVSAKKRLMPGD
LREISASEDGIPLVVQLIGHGREALVSAAQAAQDAGAVHLNLNMGCPYGRMTSGLTGGGMLKKPELLEEIIPALREAIHG
SFSIKLRAGYEDPQQVFSLLPLFERSGVDFLVLHPRTVRQAYNGWADHEVTAEVVRQTSIPVIANGDIRSAAGGQRLLEQ
TGAAGLMLGRGGIGEPMLFERLRGRAAAEPDLAERKAMLHRYLIDLLPLYCALFCGDAQVLGKIKGVVSNVEDAELEREL
KKLTRSKTLQAFRSALEELAP

Sequences:

>Translated_341_residues
MTMENPRHQALQPNSATPAPAPSKLPWLPGVKPLMLAPMQGLTNRALRSLFTKWVRPDVVWTEFMRVNPVSAKKRLMPGD
LREISASEDGIPLVVQLIGHGREALVSAAQAAQDAGAVHLNLNMGCPYGRMTSGLTGGGMLKKPELLEEIIPALREAIHG
SFSIKLRAGYEDPQQVFSLLPLFERSGVDFLVLHPRTVRQAYNGWADHEVTAEVVRQTSIPVIANGDIRSAAGGQRLLEQ
TGAAGLMLGRGGIGEPMLFERLRGRAAAEPDLAERKAMLHRYLIDLLPLYCALFCGDAQVLGKIKGVVSNVEDAELEREL
KKLTRSKTLQAFRSALEELAP
>Mature_340_residues
TMENPRHQALQPNSATPAPAPSKLPWLPGVKPLMLAPMQGLTNRALRSLFTKWVRPDVVWTEFMRVNPVSAKKRLMPGDL
REISASEDGIPLVVQLIGHGREALVSAAQAAQDAGAVHLNLNMGCPYGRMTSGLTGGGMLKKPELLEEIIPALREAIHGS
FSIKLRAGYEDPQQVFSLLPLFERSGVDFLVLHPRTVRQAYNGWADHEVTAEVVRQTSIPVIANGDIRSAAGGQRLLEQT
GAAGLMLGRGGIGEPMLFERLRGRAAAEPDLAERKAMLHRYLIDLLPLYCALFCGDAQVLGKIKGVVSNVEDAELERELK
KLTRSKTLQAFRSALEELAP

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=234, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI40807366, Length=185, Percent_Identity=31.8918918918919, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI239788483, Length=191, Percent_Identity=26.1780104712042, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI239788462, Length=191, Percent_Identity=26.1780104712042, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788462, Length=256, Percent_Identity=31.25, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1789660, Length=266, Percent_Identity=30.8270676691729, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI145693211, Length=166, Percent_Identity=26.5060240963855, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=230, Percent_Identity=26.0869565217391, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=180, Percent_Identity=32.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=198, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=24.3478260869565, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921524, Length=183, Percent_Identity=32.7868852459016, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24585320, Length=257, Percent_Identity=28.4046692607004, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580595, Length=229, Percent_Identity=26.2008733624454, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920448, Length=229, Percent_Identity=26.2008733624454, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37228; Mature: 37097

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMENPRHQALQPNSATPAPAPSKLPWLPGVKPLMLAPMQGLTNRALRSLFTKWVRPDVV
CCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
WTEFMRVNPVSAKKRLMPGDLREISASEDGIPLVVQLIGHGREALVSAAQAAQDAGAVHL
HHHHHHCCCCCHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEE
NLNMGCPYGRMTSGLTGGGMLKKPELLEEIIPALREAIHGSFSIKLRAGYEDPQQVFSLL
EEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
PLFERSGVDFLVLHPRTVRQAYNGWADHEVTAEVVRQTSIPVIANGDIRSAAGGQRLLEQ
HHHHHCCCCEEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHH
TGAAGLMLGRGGIGEPMLFERLRGRAAAEPDLAERKAMLHRYLIDLLPLYCALFCGDAQV
CCCCEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LGKIKGVVSNVEDAELERELKKLTRSKTLQAFRSALEELAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TMENPRHQALQPNSATPAPAPSKLPWLPGVKPLMLAPMQGLTNRALRSLFTKWVRPDVV
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
WTEFMRVNPVSAKKRLMPGDLREISASEDGIPLVVQLIGHGREALVSAAQAAQDAGAVHL
HHHHHHCCCCCHHHHCCCCHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCEEEE
NLNMGCPYGRMTSGLTGGGMLKKPELLEEIIPALREAIHGSFSIKLRAGYEDPQQVFSLL
EEECCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHH
PLFERSGVDFLVLHPRTVRQAYNGWADHEVTAEVVRQTSIPVIANGDIRSAAGGQRLLEQ
HHHHHCCCCEEEECCHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHH
TGAAGLMLGRGGIGEPMLFERLRGRAAAEPDLAERKAMLHRYLIDLLPLYCALFCGDAQV
CCCCEEEECCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LGKIKGVVSNVEDAELERELKKLTRSKTLQAFRSALEELAP
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]