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The map label for this gene is thtR [H]

Identifier: 197116489

GI number: 197116489

Start: 110803

End: 111777

Strand: Reverse

Name: thtR [H]

Synonym: Gbem_0089

Alternate gene names: 197116489

Gene position: 111777-110803 (Counterclockwise)

Preceding gene: 197116490

Following gene: 197116486

Centisome position: 2.42

GC content: 61.64

Gene sequence:

>975_bases
ATGGCAGGCAAACATCTGGTACGGCTCCTTTCCGTTCTGGCGCTGATAATCAGCCTGGCGGGGACGTCCTGGGCGAAACA
ACAGCAGGGATACAAGGGATATGCGCGCGGCTCCGCCCTGATCACGGCAAAGGAGCTGAAGCAGCTCATCGACGCGAAGG
ACAAGAAACTGGTGATCATCGCGGCGGAGAATGACGTCGAATACCGCCTCGGCCACATACCTGGATCGTTCCAGGTGGAC
CGCCCTGCCTACGAGGCCCCCGCGGCGACGCAAAACGGGGTGACGGGCAACCTGATCGACGCAGCAGGTTTCACCAAGCT
GGCGCAGGAGCTCGGCATCAACAAGGATTCCAAGGTCGTAGTCTACGACACCAAGTACGACGCGACGCGCCTCTGGTGGG
CCTTCCTGTACTACGGCAAAGGTGACGTGAGGGTGCTTGACGGCGGGATCAAGGCCTGGAAGGACGCCGGTTACGACACG
GCGATCCTGGCGCCGGATGCAGCCAGGGCCGGGGCCTTCGTGGCCAAGGTCGCCCTGCCGCGCAGCAGGGTGGAGACCCC
GGAGATCCTCGCGCTGAAAGACCCGGCCAAGGGACAACTCTGGGATAACCGCGACCGCAAGGAGTACTGCGGCGAGGAGT
TGAAGAAAGGGGCTTACCGGAAAGGGCGCATCCCCTGGGGCAAGCATAGCGACTGGGCGATCTTCAAGAAGAAGGAGAAC
GCAGGCGAGTGGCTCACTGCGGCCGAGGCGCAGGCGATTTTGAAAAAGCTGGGTGTGGACGGCAAGAAAGATCAGTATTT
CTTCTGCCAGTCTGGAGTGCGCTCCACCCAGGCGATGTTCACCTTTTACCTGCTTGGCTACCCGCTGAACAAGCTGCACA
ACTACGACAGCTCCTGGATCGGCTGGTCCAAGGATGCTAGCCTCCCCATCGAGACCGGGTGCGACCTGAACCCTGCGGTC
GCCACGGCGAGATAG

Upstream 100 bases:

>100_bases
AACCCTACCAAAACAGTTTAAAAAGCTCTTGCCAAGTTGACCATTTCAATAGTATAAACCGCCTACCAACTTTTACGTAA
CTTTAGCATGGAGGTAGGCA

Downstream 100 bases:

>100_bases
ACCTGCTGGTATTTCTCGAACTCATCGCCATTTGAGCGGCAACAAAAAGAAGAGGCCCTCCGGCTACAGCCGGGGGGCCT
CTTCTTTTTGCCTGGAGAGT

Product: rhodanese homology domain pair protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MAGKHLVRLLSVLALIISLAGTSWAKQQQGYKGYARGSALITAKELKQLIDAKDKKLVIIAAENDVEYRLGHIPGSFQVD
RPAYEAPAATQNGVTGNLIDAAGFTKLAQELGINKDSKVVVYDTKYDATRLWWAFLYYGKGDVRVLDGGIKAWKDAGYDT
AILAPDAARAGAFVAKVALPRSRVETPEILALKDPAKGQLWDNRDRKEYCGEELKKGAYRKGRIPWGKHSDWAIFKKKEN
AGEWLTAAEAQAILKKLGVDGKKDQYFFCQSGVRSTQAMFTFYLLGYPLNKLHNYDSSWIGWSKDASLPIETGCDLNPAV
ATAR

Sequences:

>Translated_324_residues
MAGKHLVRLLSVLALIISLAGTSWAKQQQGYKGYARGSALITAKELKQLIDAKDKKLVIIAAENDVEYRLGHIPGSFQVD
RPAYEAPAATQNGVTGNLIDAAGFTKLAQELGINKDSKVVVYDTKYDATRLWWAFLYYGKGDVRVLDGGIKAWKDAGYDT
AILAPDAARAGAFVAKVALPRSRVETPEILALKDPAKGQLWDNRDRKEYCGEELKKGAYRKGRIPWGKHSDWAIFKKKEN
AGEWLTAAEAQAILKKLGVDGKKDQYFFCQSGVRSTQAMFTFYLLGYPLNKLHNYDSSWIGWSKDASLPIETGCDLNPAV
ATAR
>Mature_323_residues
AGKHLVRLLSVLALIISLAGTSWAKQQQGYKGYARGSALITAKELKQLIDAKDKKLVIIAAENDVEYRLGHIPGSFQVDR
PAYEAPAATQNGVTGNLIDAAGFTKLAQELGINKDSKVVVYDTKYDATRLWWAFLYYGKGDVRVLDGGIKAWKDAGYDTA
ILAPDAARAGAFVAKVALPRSRVETPEILALKDPAKGQLWDNRDRKEYCGEELKKGAYRKGRIPWGKHSDWAIFKKKENA
GEWLTAAEAQAILKKLGVDGKKDQYFFCQSGVRSTQAMFTFYLLGYPLNKLHNYDSSWIGWSKDASLPIETGCDLNPAVA
TAR

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=247, Percent_Identity=28.3400809716599, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI87082121, Length=251, Percent_Identity=28.2868525896414, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081967, Length=255, Percent_Identity=27.0588235294118, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 35765; Mature: 35634

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKHLVRLLSVLALIISLAGTSWAKQQQGYKGYARGSALITAKELKQLIDAKDKKLVII
CCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCCEEEEE
AAENDVEYRLGHIPGSFQVDRPAYEAPAATQNGVTGNLIDAAGFTKLAQELGINKDSKVV
EECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCCCEEE
VYDTKYDATRLWWAFLYYGKGDVRVLDGGIKAWKDAGYDTAILAPDAARAGAFVAKVALP
EEECCCCHHHHEEEHEEECCCCEEEEECCHHHHHCCCCCEEEECCCCHHHHHHHHHHHCC
RSRVETPEILALKDPAKGQLWDNRDRKEYCGEELKKGAYRKGRIPWGKHSDWAIFKKKEN
HHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
AGEWLTAAEAQAILKKLGVDGKKDQYFFCQSGVRSTQAMFTFYLLGYPLNKLHNYDSSWI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCC
GWSKDASLPIETGCDLNPAVATAR
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AGKHLVRLLSVLALIISLAGTSWAKQQQGYKGYARGSALITAKELKQLIDAKDKKLVII
CHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHCCCCCEEEEE
AAENDVEYRLGHIPGSFQVDRPAYEAPAATQNGVTGNLIDAAGFTKLAQELGINKDSKVV
EECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHCCCCCCEEE
VYDTKYDATRLWWAFLYYGKGDVRVLDGGIKAWKDAGYDTAILAPDAARAGAFVAKVALP
EEECCCCHHHHEEEHEEECCCCEEEEECCHHHHHCCCCCEEEECCCCHHHHHHHHHHHCC
RSRVETPEILALKDPAKGQLWDNRDRKEYCGEELKKGAYRKGRIPWGKHSDWAIFKKKEN
HHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCC
AGEWLTAAEAQAILKKLGVDGKKDQYFFCQSGVRSTQAMFTFYLLGYPLNKLHNYDSSWI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCHHHHCCCCCCCC
GWSKDASLPIETGCDLNPAVATAR
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626; 8772169 [H]