Click here to switch to the map view.

The map label for this gene is rpfG [H]

Identifier: 197116480

GI number: 197116480

Start: 95859

End: 96932

Strand: Direct

Name: rpfG [H]

Synonym: Gbem_0080

Alternate gene names: 197116480

Gene position: 95859-96932 (Clockwise)

Preceding gene: 197116479

Following gene: 197116481

Centisome position: 2.08

GC content: 61.17

Gene sequence:

>1074_bases
ATGGAAAATTTAAAATCGGGGCGGGTCGTCCTCGTGGACGACGACCCATACGTGCTGGAGAGCGTGGCACTGCTCCTTTC
GGTAAGCGGATTCGAGGTCACCTCGTTTTCCAACGGCCACGAGGCGCTGCAGCTGCTGCGCCGGTCGCCGCCGGACGTGG
TGCTCACTGACGTCAACATGCCGCAGATGTCGGGCATCGAACTCCTGGAGCAGATCCACGACTTCGACCGCGACATCCCG
GTCATCCTGATGACGGCCTATGCCGAGCTGGAGATGGCCGTTTCCGCCATCAAGAAAGGCGCCTTCGACTTCATCATCAA
GCCTTTCAAGACTCCATACCTGATCTACGCCGTGGAGAAGGGGATCAACTATCAGCGCCTGGTCCTGATGGAGAAAAATT
ACAAGGCGGAGCTGGAACAAAAGGTACAGGACCGGACCCGCGAGCTGGGCGCAGCGCTGGTGCAGATGCGGAATATGAGC
CGCGAGACCATCGAGAGGCTGACGGCAGCGGCCGAACTCAGGGACGAGGACACCGGCAGGCACATCTCGCGCATCGGTCT
CTACGCCAAGAGGCTTGCCGAGCAGCTGCAAATGCCCGCGGATTTCGCTGAGGGGATCGGCATGGCTGCGCCGATGCACG
ACGTCGGCAAGATCGGCATACCCGACGCGATACTTTTGAAGGCGGGGCCGCTCAACGGTGAGGAATTCGGCGTCATGAAG
AGCCATACCGAGATCGGCGGCAGGATACTCGCCGGCTCGACTCACATGGTGCTGCAGATGGCGGCCTCCATCGCCCTTAC
CCACCACGAGCGCTGGGACGGCAGCGGCTACCCCCACGGCCTCGCCGGAGAAAAGATCCCCGTGGAGGGGCGCATCGTCA
TGCTTGTGGACCAGTACGACGCCCTCAGAAGCAAGCGCGTCTACAAGCCCACCTTCGAACACGGAAAGACGGTCCGCATC
ATCACCGAAGGGGACGGCAGAACCATGCCGGAGCATTTCGACCCGGAGATACTGCGCGCCTTCGTTACGGCGGCACACGA
CTTCGAGGCCATCTACGAGAACCATAGCGATTAG

Upstream 100 bases:

>100_bases
GCTTGGGAACGCCCTTAGCAATCTCTTCCTCAAGTTCCCATTAGAAATGCCGAAACAGGAAAAAGCACCGGACATCAGGT
GGCTAGCTGGGGAGAACTAC

Downstream 100 bases:

>100_bases
CGGGGAACCAGACGAAACATGTCCAGGTGCAGCCTGAAAATAAAGATGAGCTTGGTCGTCTTCCTGCTGATTGGTACGGT
GAGCTCGTGCGTTGCCGGCC

Product: response receiver-modulated cyclic diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MENLKSGRVVLVDDDPYVLESVALLLSVSGFEVTSFSNGHEALQLLRRSPPDVVLTDVNMPQMSGIELLEQIHDFDRDIP
VILMTAYAELEMAVSAIKKGAFDFIIKPFKTPYLIYAVEKGINYQRLVLMEKNYKAELEQKVQDRTRELGAALVQMRNMS
RETIERLTAAAELRDEDTGRHISRIGLYAKRLAEQLQMPADFAEGIGMAAPMHDVGKIGIPDAILLKAGPLNGEEFGVMK
SHTEIGGRILAGSTHMVLQMAASIALTHHERWDGSGYPHGLAGEKIPVEGRIVMLVDQYDALRSKRVYKPTFEHGKTVRI
ITEGDGRTMPEHFDPEILRAFVTAAHDFEAIYENHSD

Sequences:

>Translated_357_residues
MENLKSGRVVLVDDDPYVLESVALLLSVSGFEVTSFSNGHEALQLLRRSPPDVVLTDVNMPQMSGIELLEQIHDFDRDIP
VILMTAYAELEMAVSAIKKGAFDFIIKPFKTPYLIYAVEKGINYQRLVLMEKNYKAELEQKVQDRTRELGAALVQMRNMS
RETIERLTAAAELRDEDTGRHISRIGLYAKRLAEQLQMPADFAEGIGMAAPMHDVGKIGIPDAILLKAGPLNGEEFGVMK
SHTEIGGRILAGSTHMVLQMAASIALTHHERWDGSGYPHGLAGEKIPVEGRIVMLVDQYDALRSKRVYKPTFEHGKTVRI
ITEGDGRTMPEHFDPEILRAFVTAAHDFEAIYENHSD
>Mature_357_residues
MENLKSGRVVLVDDDPYVLESVALLLSVSGFEVTSFSNGHEALQLLRRSPPDVVLTDVNMPQMSGIELLEQIHDFDRDIP
VILMTAYAELEMAVSAIKKGAFDFIIKPFKTPYLIYAVEKGINYQRLVLMEKNYKAELEQKVQDRTRELGAALVQMRNMS
RETIERLTAAAELRDEDTGRHISRIGLYAKRLAEQLQMPADFAEGIGMAAPMHDVGKIGIPDAILLKAGPLNGEEFGVMK
SHTEIGGRILAGSTHMVLQMAASIALTHHERWDGSGYPHGLAGEKIPVEGRIVMLVDQYDALRSKRVYKPTFEHGKTVRI
ITEGDGRTMPEHFDPEILRAFVTAAHDFEAIYENHSD

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=41.3223140495868, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=40.495867768595, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790437, Length=116, Percent_Identity=37.9310344827586, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788905, Length=118, Percent_Identity=33.8983050847458, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=31.5789473684211, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789402, Length=102, Percent_Identity=31.3725490196078, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788394, Length=105, Percent_Identity=30.4761904761905, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790860, Length=101, Percent_Identity=33.6633663366337, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 39854; Mature: 39854

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLKSGRVVLVDDDPYVLESVALLLSVSGFEVTSFSNGHEALQLLRRSPPDVVLTDVNM
CCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCEEEEECCC
PQMSGIELLEQIHDFDRDIPVILMTAYAELEMAVSAIKKGAFDFIIKPFKTPYLIYAVEK
CCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEC
GINYQRLVLMEKNYKAELEQKVQDRTRELGAALVQMRNMSRETIERLTAAAELRDEDTGR
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCH
HISRIGLYAKRLAEQLQMPADFAEGIGMAAPMHDVGKIGIPDAILLKAGPLNGEEFGVMK
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCC
SHTEIGGRILAGSTHMVLQMAASIALTHHERWDGSGYPHGLAGEKIPVEGRIVMLVDQYD
CCHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHH
ALRSKRVYKPTFEHGKTVRIITEGDGRTMPEHFDPEILRAFVTAAHDFEAIYENHSD
HHHHCCCCCCHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENLKSGRVVLVDDDPYVLESVALLLSVSGFEVTSFSNGHEALQLLRRSPPDVVLTDVNM
CCCCCCCCEEEECCCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCEEEEECCC
PQMSGIELLEQIHDFDRDIPVILMTAYAELEMAVSAIKKGAFDFIIKPFKTPYLIYAVEK
CCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEEEEEC
GINYQRLVLMEKNYKAELEQKVQDRTRELGAALVQMRNMSRETIERLTAAAELRDEDTGR
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCH
HISRIGLYAKRLAEQLQMPADFAEGIGMAAPMHDVGKIGIPDAILLKAGPLNGEEFGVMK
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHCCCCCCCCEEEEECCCCCCCCCCCCC
SHTEIGGRILAGSTHMVLQMAASIALTHHERWDGSGYPHGLAGEKIPVEGRIVMLVDQYD
CCHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHH
ALRSKRVYKPTFEHGKTVRIITEGDGRTMPEHFDPEILRAFVTAAHDFEAIYENHSD
HHHHCCCCCCHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]