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The map label for this gene is cooH [H]

Identifier: 197116469

GI number: 197116469

Start: 84214

End: 85293

Strand: Direct

Name: cooH [H]

Synonym: Gbem_0069

Alternate gene names: 197116469

Gene position: 84214-85293 (Clockwise)

Preceding gene: 197116468

Following gene: 197116470

Centisome position: 1.82

GC content: 67.13

Gene sequence:

>1080_bases
ATGAGCAGCGTCATCGAACTTGGGCCCCACCAACTGGCCCTCGAGGAGCCGATGTACTTCCGGGTGAAGCTGGAGGGGGA
TCGCGTGGCGGGGCTGGACGTGCAGCCGGGGCAGGTGCACCGGGGCATGGAGCACCTGGTGATGCGCAGGAACCTCTACC
AGAACATCACCCTCTTGGAGCGCCTCTGCTCGCTTTGCTCCAACAGCCACCCGAGCACCTTCTGCTCCGCCATCGAGGAG
GTGGCGGGGGTGGCGATTCCCGAGCGGGCCGAATACCTGCGCGCCGTGGCGGACGAGATCAAGCGCATCGCGTCGCATCT
CTTTTGCTGCGCCGTGCAGGCGAACCTGATCGGCCAGACGGCGCTCTTCAGGCGGGCGATGGAAGTCCGGGAGATCATGC
AGCGCTCCAAGGAGGCGATCTACGGCAACCGCATGGACCTCGCGGCGAACTGCATCGGCGGGGTGCGCTACGACCTGACG
CCGGACGCGGCAATGTACCTCGCCCGGCAGATGGAGGCCATGAAAGCGCCGCTGGACGAGCTGTACCTGCAGTTCCAGAA
CAACCCGGCGATCATGGCGCGGACCGCAGGCGTTGGCGTCTTGCCCAGGCTTGAGGCGATCGAGTGCGCGGTGGTGGGTC
CCGTGGCGCGCGCCTGCGGCATCGACTACGACGTGCGCAAGAAGAGCCCCTACGGTGCCTATGACCGGCTGGAGTTCAGT
ACCTCCCTGCAGCAGGCGGGGGACGTCCGGGCCCGGAACCTGGTCCGGCTTGCGGAGGCGCGGCAGTCGGTCTCTCTCAT
CGAGCAGTGCCTGGCGGGGCTGGTACCCGGGCCGCTTTGCGCAGACCTCCTCCCTGAGATTCCGGCGGGGGAGGCGGTCG
CCAAGTCGGAGGCGCCGCGGGGCGAGCTGGTCTATTACCTGAGGACCGACGGCACCGACTTCCCGCTGCGTCTGAAATGG
CGGGTCCCTTCCTACATGAACTGGGACGCGCTCAAGGTGATGCTCCACGGCGAGCCGGTGGCGAACATCCCGCTCATCGT
GAACAGCATCGACCCCTGCATCGCCTGCACGGACCGCTAG

Upstream 100 bases:

>100_bases
CCGACGCCCCGTTCGAACGGCTCATCCCTTATTCCACCTTCACCAACGGCGCCGGAGGGAAAGCGCTCTGGGAAAAGGTG
CTCGCCGCCAAGGAGAAGCC

Downstream 100 bases:

>100_bases
GAGGAGCCATGCACGAAATCACCCTGGTCACCGGACTCTTCGAGATCATCAACCGCGAGGTCGCCGCGCGCGGCATCGAA
TCCATCTCCAAGGTGCGCCT

Product: carbon monoxide-induced hydrogenase large subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSSVIELGPHQLALEEPMYFRVKLEGDRVAGLDVQPGQVHRGMEHLVMRRNLYQNITLLERLCSLCSNSHPSTFCSAIEE
VAGVAIPERAEYLRAVADEIKRIASHLFCCAVQANLIGQTALFRRAMEVREIMQRSKEAIYGNRMDLAANCIGGVRYDLT
PDAAMYLARQMEAMKAPLDELYLQFQNNPAIMARTAGVGVLPRLEAIECAVVGPVARACGIDYDVRKKSPYGAYDRLEFS
TSLQQAGDVRARNLVRLAEARQSVSLIEQCLAGLVPGPLCADLLPEIPAGEAVAKSEAPRGELVYYLRTDGTDFPLRLKW
RVPSYMNWDALKVMLHGEPVANIPLIVNSIDPCIACTDR

Sequences:

>Translated_359_residues
MSSVIELGPHQLALEEPMYFRVKLEGDRVAGLDVQPGQVHRGMEHLVMRRNLYQNITLLERLCSLCSNSHPSTFCSAIEE
VAGVAIPERAEYLRAVADEIKRIASHLFCCAVQANLIGQTALFRRAMEVREIMQRSKEAIYGNRMDLAANCIGGVRYDLT
PDAAMYLARQMEAMKAPLDELYLQFQNNPAIMARTAGVGVLPRLEAIECAVVGPVARACGIDYDVRKKSPYGAYDRLEFS
TSLQQAGDVRARNLVRLAEARQSVSLIEQCLAGLVPGPLCADLLPEIPAGEAVAKSEAPRGELVYYLRTDGTDFPLRLKW
RVPSYMNWDALKVMLHGEPVANIPLIVNSIDPCIACTDR
>Mature_358_residues
SSVIELGPHQLALEEPMYFRVKLEGDRVAGLDVQPGQVHRGMEHLVMRRNLYQNITLLERLCSLCSNSHPSTFCSAIEEV
AGVAIPERAEYLRAVADEIKRIASHLFCCAVQANLIGQTALFRRAMEVREIMQRSKEAIYGNRMDLAANCIGGVRYDLTP
DAAMYLARQMEAMKAPLDELYLQFQNNPAIMARTAGVGVLPRLEAIECAVVGPVARACGIDYDVRKKSPYGAYDRLEFST
SLQQAGDVRARNLVRLAEARQSVSLIEQCLAGLVPGPLCADLLPEIPAGEAVAKSEAPRGELVYYLRTDGTDFPLRLKWR
VPSYMNWDALKVMLHGEPVANIPLIVNSIDPCIACTDR

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=383, Percent_Identity=28.1984334203655, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI260898743, Length=373, Percent_Identity=28.1501340482574, Blast_Score=144, Evalue=9e-35,
Organism=Escherichia coli, GI1789076, Length=359, Percent_Identity=32.5905292479109, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788832, Length=357, Percent_Identity=31.0924369747899, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI145693162, Length=386, Percent_Identity=27.720207253886, Blast_Score=148, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17555284, Length=383, Percent_Identity=27.4151436031332, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17568379, Length=385, Percent_Identity=28.051948051948, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI221459469, Length=385, Percent_Identity=27.2727272727273, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24638644, Length=383, Percent_Identity=26.8929503916449, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 39747; Mature: 39615

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVIELGPHQLALEEPMYFRVKLEGDRVAGLDVQPGQVHRGMEHLVMRRNLYQNITLLE
CCCCHHCCCCCEEECCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLCSLCSNSHPSTFCSAIEEVAGVAIPERAEYLRAVADEIKRIASHLFCCAVQANLIGQT
HHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFRRAMEVREIMQRSKEAIYGNRMDLAANCIGGVRYDLTPDAAMYLARQMEAMKAPLDE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCHHH
LYLQFQNNPAIMARTAGVGVLPRLEAIECAVVGPVARACGIDYDVRKKSPYGAYDRLEFS
HHEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
TSLQQAGDVRARNLVRLAEARQSVSLIEQCLAGLVPGPLCADLLPEIPAGEAVAKSEAPR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHCCCCC
GELVYYLRTDGTDFPLRLKWRVPSYMNWDALKVMLHGEPVANIPLIVNSIDPCIACTDR
CCEEEEEECCCCCCCEEEEECCCCCCCHHHEEEEECCCCCCCCCEEEECCCCCEEECCC
>Mature Secondary Structure 
SSVIELGPHQLALEEPMYFRVKLEGDRVAGLDVQPGQVHRGMEHLVMRRNLYQNITLLE
CCCHHCCCCCEEECCCEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLCSLCSNSHPSTFCSAIEEVAGVAIPERAEYLRAVADEIKRIASHLFCCAVQANLIGQT
HHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALFRRAMEVREIMQRSKEAIYGNRMDLAANCIGGVRYDLTPDAAMYLARQMEAMKAPLDE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHCCHHH
LYLQFQNNPAIMARTAGVGVLPRLEAIECAVVGPVARACGIDYDVRKKSPYGAYDRLEFS
HHEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
TSLQQAGDVRARNLVRLAEARQSVSLIEQCLAGLVPGPLCADLLPEIPAGEAVAKSEAPR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHCCCCC
GELVYYLRTDGTDFPLRLKWRVPSYMNWDALKVMLHGEPVANIPLIVNSIDPCIACTDR
CCEEEEEECCCCCCCEEEEECCCCCCCHHHEEEEECCCCCCCCCEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]