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The map label for this gene is cooL [H]

Identifier: 197116467

GI number: 197116467

Start: 82695

End: 83693

Strand: Direct

Name: cooL [H]

Synonym: Gbem_0067

Alternate gene names: 197116467

Gene position: 82695-83693 (Clockwise)

Preceding gene: 197116466

Following gene: 197116468

Centisome position: 1.79

GC content: 66.27

Gene sequence:

>999_bases
ATGCAAGACCAGGTAAAAGAAACTCCAGCGGAGCCGGTACGGCTTTTTCGCATCAATACCGGTTCCTGCAACGGATGCGA
CGTGGAGCTGGCGGTCACCGCTGCGGTGGACGAGTTCGACGTGGAGCAGTTGGGGTGCCGCTATACGGAGGAGCCGCATG
AGGCGGACATACTCCTTATCACCGGCCCCCTTACCGTCCGCGCCCGGAACGAGGCGCTGCGACTCTACGCAGAGGCGCCG
GAAACCAAGGTGACGGTGGCGGTCGGCGTCTGCCCGGTCTCCGGTGGGGTCTTCCGCGACAGCTATGCGGTCGACGGCCC
GGTGCAGCGTTACCTGCCGGTGGACCTCAACGTGCCGGGATGCCCGCCCCGTCCCCAGGCTCTCCTGGCCGGGATCGCCC
AGGCGCGGGATCTCTGGCGCGCCCGCAGCAAGGGAGTGGATGCCGTATTTGCGGCGCCCCCCCTGGCCAAGCCGGCGGAT
GAACCGGCGCAGCAATTCCCCATCCGCGGCCGGATGAGCTTTGACCCCAAGGCCTGCGTGGACTGCCGCATGTGCCGGCA
CGTCTGCGCCGCCGGGGCTATTCACTTCGATATCAAGGGGGACAAGGGGGACAGGCACCTGCGGAGCCAGTCCCCTAGCG
GCAACAAGGAGGACAGGCACCTGCGGAGCCTGTCCCCCGGCTCGCCAGAGGGGGTTCGGCTCACGCTCTGGCACAACAGC
TGTGTTTTTTGCGGCCTCTGCAGCCATTACTGCCCGACCGGCGCCTTGTCGGTGACCGGCGATTGGCATCTTGCCCACCG
CGGCGGGCAAACCTTCCGCTTCTCCGAGCAGGGAGAGGTCCCTTATACCCCCTGCTCCGGTTGCGGTTCTCCCCTCATTG
CCGTGGCCCCGGAACTCCTGCACGCGGCCTTTCGCCGCCCTACCCCCGAAATCGAACACCTTAAGACCCTCTGTCCGGCC
TGCCGGCAACAACTGAGCATAGGAGCAGTGAGACGATGA

Upstream 100 bases:

>100_bases
AGACCGCCGCCCGATTTTGGCCTACATTGCCTGCCAACCCCGGTGATCCTTCTCATCCGGGACCACGCAGACAAGCCGTT
TGCGCCAGTCGGGAGAACCC

Downstream 100 bases:

>100_bases
CCACGTTGATCGATGAGGTTGCCGCCCTCCTCGCCGCTGCGGCGGGGGAAGAGACCGTCGCCACCTGGCGCCGGGACCGG
AAGGGGGGCCTGACCGGCTG

Product: iron-sulfur cluster-binding domain-containing hydrogenase subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MQDQVKETPAEPVRLFRINTGSCNGCDVELAVTAAVDEFDVEQLGCRYTEEPHEADILLITGPLTVRARNEALRLYAEAP
ETKVTVAVGVCPVSGGVFRDSYAVDGPVQRYLPVDLNVPGCPPRPQALLAGIAQARDLWRARSKGVDAVFAAPPLAKPAD
EPAQQFPIRGRMSFDPKACVDCRMCRHVCAAGAIHFDIKGDKGDRHLRSQSPSGNKEDRHLRSLSPGSPEGVRLTLWHNS
CVFCGLCSHYCPTGALSVTGDWHLAHRGGQTFRFSEQGEVPYTPCSGCGSPLIAVAPELLHAAFRRPTPEIEHLKTLCPA
CRQQLSIGAVRR

Sequences:

>Translated_332_residues
MQDQVKETPAEPVRLFRINTGSCNGCDVELAVTAAVDEFDVEQLGCRYTEEPHEADILLITGPLTVRARNEALRLYAEAP
ETKVTVAVGVCPVSGGVFRDSYAVDGPVQRYLPVDLNVPGCPPRPQALLAGIAQARDLWRARSKGVDAVFAAPPLAKPAD
EPAQQFPIRGRMSFDPKACVDCRMCRHVCAAGAIHFDIKGDKGDRHLRSQSPSGNKEDRHLRSLSPGSPEGVRLTLWHNS
CVFCGLCSHYCPTGALSVTGDWHLAHRGGQTFRFSEQGEVPYTPCSGCGSPLIAVAPELLHAAFRRPTPEIEHLKTLCPA
CRQQLSIGAVRR
>Mature_332_residues
MQDQVKETPAEPVRLFRINTGSCNGCDVELAVTAAVDEFDVEQLGCRYTEEPHEADILLITGPLTVRARNEALRLYAEAP
ETKVTVAVGVCPVSGGVFRDSYAVDGPVQRYLPVDLNVPGCPPRPQALLAGIAQARDLWRARSKGVDAVFAAPPLAKPAD
EPAQQFPIRGRMSFDPKACVDCRMCRHVCAAGAIHFDIKGDKGDRHLRSQSPSGNKEDRHLRSLSPGSPEGVRLTLWHNS
CVFCGLCSHYCPTGALSVTGDWHLAHRGGQTFRFSEQGEVPYTPCSGCGSPLIAVAPELLHAAFRRPTPEIEHLKTLCPA
CRQQLSIGAVRR

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=109, Percent_Identity=34.8623853211009, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1788834, Length=210, Percent_Identity=34.2857142857143, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789074, Length=124, Percent_Identity=42.741935483871, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788624, Length=108, Percent_Identity=34.2592592592593, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17509685, Length=109, Percent_Identity=34.8623853211009, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24651058, Length=109, Percent_Identity=35.7798165137615, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI18859983, Length=109, Percent_Identity=33.9449541284404, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24642371, Length=109, Percent_Identity=33.9449541284404, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 36066; Mature: 36066

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQVKETPAEPVRLFRINTGSCNGCDVELAVTAAVDEFDVEQLGCRYTEEPHEADILLI
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHCCCCCCCCCCCCEEEE
TGPLTVRARNEALRLYAEAPETKVTVAVGVCPVSGGVFRDSYAVDGPVQRYLPVDLNVPG
ECCEEEEECCCEEEEEECCCCCEEEEEEEEEECCCCEECCCCCCCCCHHHEEEEECCCCC
CPPRPQALLAGIAQARDLWRARSKGVDAVFAAPPLAKPADEPAQQFPIRGRMSFDPKACV
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHCCCCCCCCCCCHHHH
DCRMCRHVCAAGAIHFDIKGDKGDRHLRSQSPSGNKEDRHLRSLSPGSPEGVRLTLWHNS
HHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECC
CVFCGLCSHYCPTGALSVTGDWHLAHRGGQTFRFSEQGEVPYTPCSGCGSPLIAVAPELL
EEEEEHHHHCCCCCCEEECCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
HAAFRRPTPEIEHLKTLCPACRQQLSIGAVRR
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQDQVKETPAEPVRLFRINTGSCNGCDVELAVTAAVDEFDVEQLGCRYTEEPHEADILLI
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCHHHHCCCCCCCCCCCCEEEE
TGPLTVRARNEALRLYAEAPETKVTVAVGVCPVSGGVFRDSYAVDGPVQRYLPVDLNVPG
ECCEEEEECCCEEEEEECCCCCEEEEEEEEEECCCCEECCCCCCCCCHHHEEEEECCCCC
CPPRPQALLAGIAQARDLWRARSKGVDAVFAAPPLAKPADEPAQQFPIRGRMSFDPKACV
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHCCCCCCCCCCCHHHH
DCRMCRHVCAAGAIHFDIKGDKGDRHLRSQSPSGNKEDRHLRSLSPGSPEGVRLTLWHNS
HHHHHHHHHHCCCEEEEEECCCCCCHHHCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECC
CVFCGLCSHYCPTGALSVTGDWHLAHRGGQTFRFSEQGEVPYTPCSGCGSPLIAVAPELL
EEEEEHHHHCCCCCCEEECCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
HAAFRRPTPEIEHLKTLCPACRQQLSIGAVRR
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]