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The map label for this gene is hdrF [H]

Identifier: 197116426

GI number: 197116426

Start: 31964

End: 32806

Strand: Reverse

Name: hdrF [H]

Synonym: Gbem_0026

Alternate gene names: 197116426

Gene position: 32806-31964 (Counterclockwise)

Preceding gene: 197116427

Following gene: 197116425

Centisome position: 0.71

GC content: 62.28

Gene sequence:

>843_bases
ATGTGCCAATCGAACAACATCTATCTGCCTAACCTTGCCACTATCGAGGCCATCGTCGACGAAACCCCCGATGTCCGCAC
CCTGCGCCTGGTCTTCCAGGACGAGCAGGTCCGCGAGAACTTCAGCTTTCGGGCGGGACAGTTCGCCGAGTATTCAGCCT
TCGGCGCCGGCGAGTCCACCTTCTGCATCGCCTCCGCCCCCACCAGGAAAGGGTACATAGAGTGCTGCTTCCGGAGCGTG
GGAAGGGTCACCGAGTCGCTCAGGAGACTGGAGGTCGGCGATACCATCGGCGTGCGCGGCCCCTACGGCAACTCCTTCCC
CATCGAGCAGTTCTTCGGCAAGAGCCTCGTATTCATCGCCGGGGGCATCGCGCTCCCGCCGCTTAGGACCGTGATCTGGA
ACTGCCTCGACCTGCGCGACAAGTTCAAGGACATCACCATCGTCTACGGCGCCCGCTCCGAAGCGGATCTGGTCTACAAG
CGCGAGCTTGCCGAGTGGCAGGAAAGAGACGACGTGAGGCTGGTGAAGTGCGTCGATCCCGGCGGGAACGGACCGGATTT
CCACGGCAAAGTCGGCTTCGTCCCCAACGTCCTGGAGGAAGCAGCGCCCAGTTCCGAGAACACGGTAGCGCTCGTCTGCG
GACCGCCAATCATGATCAAGTTCACCCTCCCCGTGCTGGAGCGCCTAGGGTTCGCCGACGACCAGATCTACACCACGCTG
GAGAACCGGATGAAGTGCGGCGTCGGCAAATGCGGCCGCTGCAACGTCGGCAACGTTTACGTCTGCAAAGACGGCCCGGT
CTTCACCGCCGGCCAAGTAAAGGCGATGTCGCAGGAGTTCTAG

Upstream 100 bases:

>100_bases
TGGGTGAAGGTGCTATCGGCAATGCCTATGGCCGCTTCCCCCCCCACCCCGACCCTCCCCCGCAGGGGGGGAGGGAGCTT
AGAGACACGAGAGAGAAGCC

Downstream 100 bases:

>100_bases
GAAAGCCGCGGGGTCACAACAGCGAAGAGGGGGAAAGGCACAATGCCTTTCCCCCTCTTTTCGTTCTACCTGTCGCACCA
TGGCGCGCTGGCGATGAGTT

Product: heterodisulfide oxidoreductase, NAD(P)H oxidoreductase subunit

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MCQSNNIYLPNLATIEAIVDETPDVRTLRLVFQDEQVRENFSFRAGQFAEYSAFGAGESTFCIASAPTRKGYIECCFRSV
GRVTESLRRLEVGDTIGVRGPYGNSFPIEQFFGKSLVFIAGGIALPPLRTVIWNCLDLRDKFKDITIVYGARSEADLVYK
RELAEWQERDDVRLVKCVDPGGNGPDFHGKVGFVPNVLEEAAPSSENTVALVCGPPIMIKFTLPVLERLGFADDQIYTTL
ENRMKCGVGKCGRCNVGNVYVCKDGPVFTAGQVKAMSQEF

Sequences:

>Translated_280_residues
MCQSNNIYLPNLATIEAIVDETPDVRTLRLVFQDEQVRENFSFRAGQFAEYSAFGAGESTFCIASAPTRKGYIECCFRSV
GRVTESLRRLEVGDTIGVRGPYGNSFPIEQFFGKSLVFIAGGIALPPLRTVIWNCLDLRDKFKDITIVYGARSEADLVYK
RELAEWQERDDVRLVKCVDPGGNGPDFHGKVGFVPNVLEEAAPSSENTVALVCGPPIMIKFTLPVLERLGFADDQIYTTL
ENRMKCGVGKCGRCNVGNVYVCKDGPVFTAGQVKAMSQEF
>Mature_280_residues
MCQSNNIYLPNLATIEAIVDETPDVRTLRLVFQDEQVRENFSFRAGQFAEYSAFGAGESTFCIASAPTRKGYIECCFRSV
GRVTESLRRLEVGDTIGVRGPYGNSFPIEQFFGKSLVFIAGGIALPPLRTVIWNCLDLRDKFKDITIVYGARSEADLVYK
RELAEWQERDDVRLVKCVDPGGNGPDFHGKVGFVPNVLEEAAPSSENTVALVCGPPIMIKFTLPVLERLGFADDQIYTTL
ENRMKCGVGKCGRCNVGNVYVCKDGPVFTAGQVKAMSQEF

Specific function: This enzyme catalyzes the hydrogen sulfide production from sulfite. It is strictly anaerobic. It is regulated by electron acceptors rather than by cysteine [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17563926, Length=169, Percent_Identity=28.4023668639053, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322146, Length=183, Percent_Identity=27.8688524590164, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281366101, Length=179, Percent_Identity=30.1675977653631, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24662934, Length=179, Percent_Identity=30.1675977653631, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24662938, Length=179, Percent_Identity=30.1675977653631, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007804, Length=183, Percent_Identity=28.9617486338798, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI116007806, Length=183, Percent_Identity=28.9617486338798, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012165
- InterPro:   IPR019480
- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938
- InterPro:   IPR014260 [H]

Pfam domain/function: PF10418 DHODB_Fe-S_bind; PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 30954; Mature: 30954

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQSNNIYLPNLATIEAIVDETPDVRTLRLVFQDEQVRENFSFRAGQFAEYSAFGAGEST
CCCCCCEECCCHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCCCCCCHHHCCCCCCCE
FCIASAPTRKGYIECCFRSVGRVTESLRRLEVGDTIGVRGPYGNSFPIEQFFGKSLVFIA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEEEEE
GGIALPPLRTVIWNCLDLRDKFKDITIVYGARSEADLVYKRELAEWQERDDVRLVKCVDP
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECC
GGNGPDFHGKVGFVPNVLEEAAPSSENTVALVCGPPIMIKFTLPVLERLGFADDQIYTTL
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHCCCCCHHHHHHH
ENRMKCGVGKCGRCNVGNVYVCKDGPVFTAGQVKAMSQEF
HHHHHCCCCCCCCCCCCCEEEECCCCEECCCHHHHHCCCC
>Mature Secondary Structure
MCQSNNIYLPNLATIEAIVDETPDVRTLRLVFQDEQVRENFSFRAGQFAEYSAFGAGEST
CCCCCCEECCCHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCCCCCCHHHCCCCCCCE
FCIASAPTRKGYIECCFRSVGRVTESLRRLEVGDTIGVRGPYGNSFPIEQFFGKSLVFIA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCEEEEEE
GGIALPPLRTVIWNCLDLRDKFKDITIVYGARSEADLVYKRELAEWQERDDVRLVKCVDP
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECC
GGNGPDFHGKVGFVPNVLEEAAPSSENTVALVCGPPIMIKFTLPVLERLGFADDQIYTTL
CCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHHHHCCCCCHHHHHHH
ENRMKCGVGKCGRCNVGNVYVCKDGPVFTAGQVKAMSQEF
HHHHHCCCCCCCCCCCCCEEEECCCCEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1704886; 11677609 [H]