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The map label for this gene is fixK [H]

Identifier: 197106259

GI number: 197106259

Start: 3211003

End: 3211656

Strand: Direct

Name: fixK [H]

Synonym: PHZ_c2798

Alternate gene names: 197106259

Gene position: 3211003-3211656 (Clockwise)

Preceding gene: 197106254

Following gene: 197106260

Centisome position: 80.35

GC content: 69.42

Gene sequence:

>654_bases
ATGACCCTCGCCTACTCGCTGGACGACCCCCGCACCGCCCGCCCGGCCGCCGCGCTGACGGAGGTCATGGACCGCCTCGG
CACCAAGATGACCTTCGCGCGGGACGAGGAGATCTACGCCCAGGGCGACGACGTCGAATTCATGTACCGCCTGGCCAAGG
GCGTCATCCGCACCAGCCGGGTGACCGCCGACGGCCGCCGGCAGGTTGGCGACTTCTACTATCCCGGCGACCTGTTCGGC
CTGGAAGTGGGCCCCGACCACCAGTTCTCGGCAGAAGCGCTGACGGACTGCGAGATCCAGATGGTCCGCCGCGGCTCGGT
CCGCGCCTTCGCCGGCGACGCCGAGTTGGACCGGGCGATCCTGGAAGCCACCCGCCGCGAGCTGCAACGGGTTCAGGACC
ACGTCCTCCTCCTGGGCCGCAAGAGCGCCCGCGAGAAGGTCGCCGCCTTCCTGATGAGCCTGGCCCAGCGCGACCCCAGC
GCGGACGTCGAGCTGCCCATGGGCCGCCAGGACATGGCCGACTACCTGGGCCTGACCATCGAGACCGTCTCGCGCATGCT
GACGCAGCTGCAGGGCGACGCCATCGTCGAGTTCCCGTCCTTCCGCCGCTTCCACGTCAAGAAGTGGCAGGCCCTGGAAG
AACTCGTGGACTGA

Upstream 100 bases:

>100_bases
TCCGTAGAACTACTTAGGTGCGACCCCTTAGGGGGACGCCACGAATTTCTTGCGGCCCGGCGCAGGCGTACCCCTTCGTC
ATCGACGCAGGAACTTCGCC

Downstream 100 bases:

>100_bases
CCGCATCACCCCCAGCGGCCGGGGGCGTCCAGCCGGCCGCGCGAAAGCGAGGGAGGCTCACATGTCCCAGAAGCAGGGGT
CCCAGACCCAGGAACGTCCC

Product: transcriptional regulator, Crp/Fnr family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTLAYSLDDPRTARPAAALTEVMDRLGTKMTFARDEEIYAQGDDVEFMYRLAKGVIRTSRVTADGRRQVGDFYYPGDLFG
LEVGPDHQFSAEALTDCEIQMVRRGSVRAFAGDAELDRAILEATRRELQRVQDHVLLLGRKSAREKVAAFLMSLAQRDPS
ADVELPMGRQDMADYLGLTIETVSRMLTQLQGDAIVEFPSFRRFHVKKWQALEELVD

Sequences:

>Translated_217_residues
MTLAYSLDDPRTARPAAALTEVMDRLGTKMTFARDEEIYAQGDDVEFMYRLAKGVIRTSRVTADGRRQVGDFYYPGDLFG
LEVGPDHQFSAEALTDCEIQMVRRGSVRAFAGDAELDRAILEATRRELQRVQDHVLLLGRKSAREKVAAFLMSLAQRDPS
ADVELPMGRQDMADYLGLTIETVSRMLTQLQGDAIVEFPSFRRFHVKKWQALEELVD
>Mature_216_residues
TLAYSLDDPRTARPAAALTEVMDRLGTKMTFARDEEIYAQGDDVEFMYRLAKGVIRTSRVTADGRRQVGDFYYPGDLFGL
EVGPDHQFSAEALTDCEIQMVRRGSVRAFAGDAELDRAILEATRRELQRVQDHVLLLGRKSAREKVAAFLMSLAQRDPSA
DVELPMGRQDMADYLGLTIETVSRMLTQLQGDAIVEFPSFRRFHVKKWQALEELVD

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK is a positive regulator of nifA expression. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=176, Percent_Identity=30.6818181818182, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24538; Mature: 24407

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAYSLDDPRTARPAAALTEVMDRLGTKMTFARDEEIYAQGDDVEFMYRLAKGVIRTSR
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHCCCCCHHHHHHHHHHHHHHHH
VTADGRRQVGDFYYPGDLFGLEVGPDHQFSAEALTDCEIQMVRRGSVRAFAGDAELDRAI
CCCCCHHHCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHH
LEATRRELQRVQDHVLLLGRKSAREKVAAFLMSLAQRDPSADVELPMGRQDMADYLGLTI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ETVSRMLTQLQGDAIVEFPSFRRFHVKKWQALEELVD
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TLAYSLDDPRTARPAAALTEVMDRLGTKMTFARDEEIYAQGDDVEFMYRLAKGVIRTSR
EEEECCCCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHCCCCCHHHHHHHHHHHHHHHH
VTADGRRQVGDFYYPGDLFGLEVGPDHQFSAEALTDCEIQMVRRGSVRAFAGDAELDRAI
CCCCCHHHCCCEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHH
LEATRRELQRVQDHVLLLGRKSAREKVAAFLMSLAQRDPSADVELPMGRQDMADYLGLTI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
ETVSRMLTQLQGDAIVEFPSFRRFHVKKWQALEELVD
HHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766374 [H]