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The map label for this gene is gdhA [H]

Identifier: 197105895

GI number: 197105895

Start: 2778866

End: 2780137

Strand: Reverse

Name: gdhA [H]

Synonym: PHZ_c2434

Alternate gene names: 197105895

Gene position: 2780137-2778866 (Counterclockwise)

Preceding gene: 197105896

Following gene: 197105894

Centisome position: 69.57

GC content: 64.86

Gene sequence:

>1272_bases
ATGCAAGCCCAAGGACTTTACGCCGGTCCTGTCTTCGACATGGCGCGGAGCCAGTTCGAGCAGGTCGCGGATCATCTCGG
CATCGCCTCCGATGAGCGACCGCGCCTGCTTTACCCGAAGAGAGCCATCTCGGTGTCGGTCCCGATCCACAGGGATGACG
GTCGCACAGAGGTCTTTCAGGGTTTCAGGGTGCAACATCATCTCACTCGCGGGCCTGGCAAAGGGGGGACACGGTTCGCA
CCTCAACTCGAAATGGGCGAGGTTGCCGCCCTTGCTGTCTGGATGAGCTGGAAGTGCGCTCTTGCGGATCTGCCCTTCGG
CGGCGCCAAGGGCGGCGTCAACTGCGATCCCAGGGCCCTGTCGCGGCGCGAACTCGAGGCCGTATCTCGCCGCTACATGC
AGGAGATGATCCCGTTTGTCGGCCCAAAGACCGATGTCATGGCGCCGGATGTCGGGACCGACGAGACGGTGATGGGCTGG
TTCGTCGACACCTATTCGATGCACCAGGGCTTCACGGCCAATGAGGTGGTCACGGGCAAACCAGTAGGGCTCGGCGGCAC
GGCCGGACGGCGCGCCGCCACAGGCCGCGGCGTGGCCCTGACGATCGGCGAGGCGATGAAGGTGATCGGCCTTCCGCCCG
TCGGCGCGACAGCAATCGTCCAGGGCTTCGGCAACGTAGGTTCAGTAACCGCGGAAATTCTCTCCGAAGAGATGGGCCTG
CGGGTACTTGGCGTCAGCGACCATTCCGCCGCCTACTTCCACCCTGACGGCCTGCCGGTCGCCGAGCTCATCCAGCACGT
GAAGCGTCACGGTGCTCTGACAGGCTGGTCCAACGAGCTGCTCACGGATCCGCGGGAGCTTCTGACCCGGCCCTGTGATG
TGCTTGTTCCCGCCGCTGTCGAACGCGTGATTGACGCCGACATCGCACGCCGGCTGCAGTGCCGCATCCTGGCCGAGGGC
GCCAACGGACCCACGACGCCCGAGGCTGATGTCGTCCTCGATCAGCGCCGCGGCGAGGTGTTCGTGATCCCGGACATCCT
CTGCAACAGCGGCGGAGTGATCGTCTCCTATTTCGAGTGGGTGCAGGGCCTGCAGCGCCTGTTCTGGTCTGAGGACGAGG
TGAACGACAGCCTCGCCCGGCAGATGTTCAAGGCTTTCGCCAACGTGGTCTCCCGGGCGCAACGCGACGGCATTTCCCAT
CGCATGGCCGCCACAGCCATCGGCGTCGAACACGTGCTCCTCAGCAAGCTGCAGCGCGGCTTGTTCCCCTGA

Upstream 100 bases:

>100_bases
CCTCGCCGGCGGCCCTCGTTGCGGTCGTTCTCGTCATGCACGCCTTCGCCCGCCGGTTCCGCAGGCCGGCGAACGGCTCT
TCGAAAGCAGGAAACAGTTG

Downstream 100 bases:

>100_bases
GGGAGCCTGGCCGTCTCCGCTCCTCCCCGGCCAGGAAACCTTGCCACCCGGCTGCGGCCGGGTGGCCCCTTTTTCATCGC
AGCCAGAATGCCGGCCCATG

Product: Glu/Leu/Phe/Val dehydrogenase

Products: NA

Alternate protein names: GDH [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MQAQGLYAGPVFDMARSQFEQVADHLGIASDERPRLLYPKRAISVSVPIHRDDGRTEVFQGFRVQHHLTRGPGKGGTRFA
PQLEMGEVAALAVWMSWKCALADLPFGGAKGGVNCDPRALSRRELEAVSRRYMQEMIPFVGPKTDVMAPDVGTDETVMGW
FVDTYSMHQGFTANEVVTGKPVGLGGTAGRRAATGRGVALTIGEAMKVIGLPPVGATAIVQGFGNVGSVTAEILSEEMGL
RVLGVSDHSAAYFHPDGLPVAELIQHVKRHGALTGWSNELLTDPRELLTRPCDVLVPAAVERVIDADIARRLQCRILAEG
ANGPTTPEADVVLDQRRGEVFVIPDILCNSGGVIVSYFEWVQGLQRLFWSEDEVNDSLARQMFKAFANVVSRAQRDGISH
RMAATAIGVEHVLLSKLQRGLFP

Sequences:

>Translated_423_residues
MQAQGLYAGPVFDMARSQFEQVADHLGIASDERPRLLYPKRAISVSVPIHRDDGRTEVFQGFRVQHHLTRGPGKGGTRFA
PQLEMGEVAALAVWMSWKCALADLPFGGAKGGVNCDPRALSRRELEAVSRRYMQEMIPFVGPKTDVMAPDVGTDETVMGW
FVDTYSMHQGFTANEVVTGKPVGLGGTAGRRAATGRGVALTIGEAMKVIGLPPVGATAIVQGFGNVGSVTAEILSEEMGL
RVLGVSDHSAAYFHPDGLPVAELIQHVKRHGALTGWSNELLTDPRELLTRPCDVLVPAAVERVIDADIARRLQCRILAEG
ANGPTTPEADVVLDQRRGEVFVIPDILCNSGGVIVSYFEWVQGLQRLFWSEDEVNDSLARQMFKAFANVVSRAQRDGISH
RMAATAIGVEHVLLSKLQRGLFP
>Mature_423_residues
MQAQGLYAGPVFDMARSQFEQVADHLGIASDERPRLLYPKRAISVSVPIHRDDGRTEVFQGFRVQHHLTRGPGKGGTRFA
PQLEMGEVAALAVWMSWKCALADLPFGGAKGGVNCDPRALSRRELEAVSRRYMQEMIPFVGPKTDVMAPDVGTDETVMGW
FVDTYSMHQGFTANEVVTGKPVGLGGTAGRRAATGRGVALTIGEAMKVIGLPPVGATAIVQGFGNVGSVTAEILSEEMGL
RVLGVSDHSAAYFHPDGLPVAELIQHVKRHGALTGWSNELLTDPRELLTRPCDVLVPAAVERVIDADIARRLQCRILAEG
ANGPTTPEADVVLDQRRGEVFVIPDILCNSGGVIVSYFEWVQGLQRLFWSEDEVNDSLARQMFKAFANVVSRAQRDGISH
RMAATAIGVEHVLLSKLQRGLFP

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=351, Percent_Identity=41.8803418803419, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI4885281, Length=351, Percent_Identity=41.8803418803419, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788059, Length=359, Percent_Identity=31.7548746518106, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17544676, Length=373, Percent_Identity=40.7506702412869, Blast_Score=269, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324951, Length=392, Percent_Identity=28.8265306122449, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319256, Length=393, Percent_Identity=29.2620865139949, Blast_Score=120, Evalue=5e-28,
Organism=Drosophila melanogaster, GI45549226, Length=384, Percent_Identity=39.84375, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI45553475, Length=340, Percent_Identity=42.6470588235294, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24649283, Length=365, Percent_Identity=35.0684931506849, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24649460, Length=332, Percent_Identity=40.0602409638554, Blast_Score=230, Evalue=1e-60,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.3 [H]

Molecular weight: Translated: 45857; Mature: 45857

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQGLYAGPVFDMARSQFEQVADHLGIASDERPRLLYPKRAISVSVPIHRDDGRTEVFQ
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEEECCCCHHHHHH
GFRVQHHLTRGPGKGGTRFAPQLEMGEVAALAVWMSWKCALADLPFGGAKGGVNCDPRAL
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHH
SRRELEAVSRRYMQEMIPFVGPKTDVMAPDVGTDETVMGWFVDTYSMHQGFTANEVVTGK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCC
PVGLGGTAGRRAATGRGVALTIGEAMKVIGLPPVGATAIVQGFGNVGSVTAEILSEEMGL
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCE
RVLGVSDHSAAYFHPDGLPVAELIQHVKRHGALTGWSNELLTDPRELLTRPCDVLVPAAV
EEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHH
ERVIDADIARRLQCRILAEGANGPTTPEADVVLDQRRGEVFVIPDILCNSGGVIVSYFEW
HHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCEEEECHHHHCCCCEEHHHHHH
VQGLQRLFWSEDEVNDSLARQMFKAFANVVSRAQRDGISHRMAATAIGVEHVLLSKLQRG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
LFP
CCC
>Mature Secondary Structure
MQAQGLYAGPVFDMARSQFEQVADHLGIASDERPRLLYPKRAISVSVPIHRDDGRTEVFQ
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEEECCCCHHHHHH
GFRVQHHLTRGPGKGGTRFAPQLEMGEVAALAVWMSWKCALADLPFGGAKGGVNCDPRAL
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHH
SRRELEAVSRRYMQEMIPFVGPKTDVMAPDVGTDETVMGWFVDTYSMHQGFTANEVVTGK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEECCC
PVGLGGTAGRRAATGRGVALTIGEAMKVIGLPPVGATAIVQGFGNVGSVTAEILSEEMGL
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCE
RVLGVSDHSAAYFHPDGLPVAELIQHVKRHGALTGWSNELLTDPRELLTRPCDVLVPAAV
EEEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHCCHHHHHCCCHHHHHHHHH
ERVIDADIARRLQCRILAEGANGPTTPEADVVLDQRRGEVFVIPDILCNSGGVIVSYFEW
HHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEECCCCCEEEECHHHHCCCCEEHHHHHH
VQGLQRLFWSEDEVNDSLARQMFKAFANVVSRAQRDGISHRMAATAIGVEHVLLSKLQRG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
LFP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9680336; 10360571; 9135121; 9654452; 10366510 [H]