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The map label for this gene is murD

Identifier: 197105787

GI number: 197105787

Start: 2637144

End: 2638553

Strand: Reverse

Name: murD

Synonym: PHZ_c2325

Alternate gene names: 197105787

Gene position: 2638553-2637144 (Counterclockwise)

Preceding gene: 197105788

Following gene: 197105786

Centisome position: 66.03

GC content: 72.55

Gene sequence:

>1410_bases
ATGATCCCGGTCAGCGGCTTCGAGGGACGGACGGTCGCCGTGTTCGGCCTGGCCCGCACGGGCCTGGCCGCCGCGCGCGC
GCTCGTCGCCGGCGGGGCCAAGGTGGCGCTCTGGGACGACAAGCCGGCCGCGCGAGAAGCTGCGCAGGCCGAGGGCTTCG
AGCTGACCGACCTGACCCGGGCCGACTGGCGGGAGTTCGCCGCCGTCATGCTCTCGCCCGGCGTGCCGCTGACCCATCCG
GCGCCGCACTGGACCGTCGAGCGGGCCCGCGAGGCCGGGGCCGAGATCGTGGGCGACATCGAGCTCTTCGCCCGTACCGT
CAACGCCGCGCCCGAGCACAAGCGGCCGAAGATCGTCGCCATCACCGGCACCAACGGCAAGTCCACGACCACGGCGCTGA
TCGGCCACATCTGCGCCGAGGCCGGCCGCGACGTTCGGATCGGCGGCAACATCGGCTACGGCGTGCTGGGCCTCGAGGAC
ATGCACGGCGGGGCGGTCTACGTGCTGGAGCTGTCGTCCTACCAGCTGGACCTGACGTCCTCGCTGCATGCCGACGTCAC
CGTGATCCTGAACATCTCGCCCGACCACCTCGAGCGGCACGGGACGATGGACGAGTATGTCGCCGCCAAGCGCCGCATCC
TGCTGAACCAGGGCAAGGGCGACACCGCCGTCATCGGCGTGGACGATCCCTGGGGCCAGCGGATCTGCACCGAGATCACC
GCCGCCAACCGCCGCACCATCGTGCCGGTCTCGGCGTCCAAGGCCATGAGCCGCGGGGTCTACGCCCTGGACGGCCTGCT
CTACGACGCCACCGGCGAGCGCGCCCAGGAAGTGGCCGACCTGAAGCGGGCCCGCTCGCTGCCCGGCCGCCACAACTGGC
AGAACGCGGCGGCCGCCTACGCCGCCGCCAGGGGGCTCGGGATCTCGGGCGAGGAGGCGGCCCAGCACCTGATGACCTTC
CCCGGGCTCGCGCACCGGATGGAGACCGTGGCGGTGCTGGGCAAGGTCCGCTTCGTCAACGACAGCAAGGCCACCAACGC
CGACGCCGCGCGGCAGGCGATGTCCAGCTACCCGAAGTTCTACTGGATCGCCGGCGGCCTGCCGAAGACCGGCGGCATCG
ACGGCCTCATCGACCTCTTCCCGCGCATCGAGAAGGCCTACCTGGTGGGCGAGGCCGCGCCGGCCTTCGCCGACGTGCTG
CGCGGCAAGGCGCCCGCCGTCGAAAGCGGGACCATCGAGGCCGCCGTGCGCCAGGCCTACGCGGACGCCCGGGCCGCGGG
TCAGGAGGCGATCGTGCTGCTGTCGCCGGCCTGCGCCTCGTTCGACCAGTTCGCCGACTTCGAGGAACGCGGCGAGGCCT
TCCGCGCCGCCGTCCAGAAGCTGGCCGACGGCCAGGCGGACGCCGCATGA

Upstream 100 bases:

>100_bases
GGAATCGACCGTCGTCATCCGCTTCTGGATCGTCGCCATCATGCTGGCCCTCGTGGGCCTCGCCACCCTGAAGCTCAGGT
AGGGGAGGGCGCGCGGTCAG

Downstream 100 bases:

>100_bases
GCCCGGTGAACAACGTCTCCCAGGCCCACGCCTTTCCGCGTTCGGACCGCTCGCCGCTCGGCGTGTGGTGGTGGACGGTG
GACCGCTGGATGCTGGGCGT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MIPVSGFEGRTVAVFGLARTGLAAARALVAGGAKVALWDDKPAAREAAQAEGFELTDLTRADWREFAAVMLSPGVPLTHP
APHWTVERAREAGAEIVGDIELFARTVNAAPEHKRPKIVAITGTNGKSTTTALIGHICAEAGRDVRIGGNIGYGVLGLED
MHGGAVYVLELSSYQLDLTSSLHADVTVILNISPDHLERHGTMDEYVAAKRRILLNQGKGDTAVIGVDDPWGQRICTEIT
AANRRTIVPVSASKAMSRGVYALDGLLYDATGERAQEVADLKRARSLPGRHNWQNAAAAYAAARGLGISGEEAAQHLMTF
PGLAHRMETVAVLGKVRFVNDSKATNADAARQAMSSYPKFYWIAGGLPKTGGIDGLIDLFPRIEKAYLVGEAAPAFADVL
RGKAPAVESGTIEAAVRQAYADARAAGQEAIVLLSPACASFDQFADFEERGEAFRAAVQKLADGQADAA

Sequences:

>Translated_469_residues
MIPVSGFEGRTVAVFGLARTGLAAARALVAGGAKVALWDDKPAAREAAQAEGFELTDLTRADWREFAAVMLSPGVPLTHP
APHWTVERAREAGAEIVGDIELFARTVNAAPEHKRPKIVAITGTNGKSTTTALIGHICAEAGRDVRIGGNIGYGVLGLED
MHGGAVYVLELSSYQLDLTSSLHADVTVILNISPDHLERHGTMDEYVAAKRRILLNQGKGDTAVIGVDDPWGQRICTEIT
AANRRTIVPVSASKAMSRGVYALDGLLYDATGERAQEVADLKRARSLPGRHNWQNAAAAYAAARGLGISGEEAAQHLMTF
PGLAHRMETVAVLGKVRFVNDSKATNADAARQAMSSYPKFYWIAGGLPKTGGIDGLIDLFPRIEKAYLVGEAAPAFADVL
RGKAPAVESGTIEAAVRQAYADARAAGQEAIVLLSPACASFDQFADFEERGEAFRAAVQKLADGQADAA
>Mature_469_residues
MIPVSGFEGRTVAVFGLARTGLAAARALVAGGAKVALWDDKPAAREAAQAEGFELTDLTRADWREFAAVMLSPGVPLTHP
APHWTVERAREAGAEIVGDIELFARTVNAAPEHKRPKIVAITGTNGKSTTTALIGHICAEAGRDVRIGGNIGYGVLGLED
MHGGAVYVLELSSYQLDLTSSLHADVTVILNISPDHLERHGTMDEYVAAKRRILLNQGKGDTAVIGVDDPWGQRICTEIT
AANRRTIVPVSASKAMSRGVYALDGLLYDATGERAQEVADLKRARSLPGRHNWQNAAAAYAAARGLGISGEEAAQHLMTF
PGLAHRMETVAVLGKVRFVNDSKATNADAARQAMSSYPKFYWIAGGLPKTGGIDGLIDLFPRIEKAYLVGEAAPAFADVL
RGKAPAVESGTIEAAVRQAYADARAAGQEAIVLLSPACASFDQFADFEERGEAFRAAVQKLADGQADAA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=472, Percent_Identity=34.9576271186441, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PHEZH (B4RFS2)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002131164.1
- ProteinModelPortal:   B4RFS2
- GeneID:   6791846
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c2325
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK01390
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49618; Mature: 49618

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPVSGFEGRTVAVFGLARTGLAAARALVAGGAKVALWDDKPAAREAAQAEGFELTDLTR
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEECCCH
ADWREFAAVMLSPGVPLTHPAPHWTVERAREAGAEIVGDIELFARTVNAAPEHKRPKIVA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
ITGTNGKSTTTALIGHICAEAGRDVRIGGNIGYGVLGLEDMHGGAVYVLELSSYQLDLTS
EECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCEEEEEEECCEEEEECC
SLHADVTVILNISPDHLERHGTMDEYVAAKRRILLNQGKGDTAVIGVDDPWGQRICTEIT
CCCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHH
AANRRTIVPVSASKAMSRGVYALDGLLYDATGERAQEVADLKRARSLPGRHNWQNAAAAY
HCCCEEEEEECHHHHHHCCCCHHHCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAARGLGISGEEAAQHLMTFPGLAHRMETVAVLGKVRFVNDSKATNADAARQAMSSYPKF
HHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCE
YWIAGGLPKTGGIDGLIDLFPRIEKAYLVGEAAPAFADVLRGKAPAVESGTIEAAVRQAY
EEEECCCCCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
ADARAAGQEAIVLLSPACASFDQFADFEERGEAFRAAVQKLADGQADAA
HHHHHCCCCEEEEECCCHHCHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIPVSGFEGRTVAVFGLARTGLAAARALVAGGAKVALWDDKPAAREAAQAEGFELTDLTR
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCEEECCCH
ADWREFAAVMLSPGVPLTHPAPHWTVERAREAGAEIVGDIELFARTVNAAPEHKRPKIVA
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEE
ITGTNGKSTTTALIGHICAEAGRDVRIGGNIGYGVLGLEDMHGGAVYVLELSSYQLDLTS
EECCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCEEEEEEECCEEEEECC
SLHADVTVILNISPDHLERHGTMDEYVAAKRRILLNQGKGDTAVIGVDDPWGQRICTEIT
CCCCCEEEEEECCHHHHHHCCCHHHHHHHHHHHEEECCCCCEEEEECCCCHHHHHHHHHH
AANRRTIVPVSASKAMSRGVYALDGLLYDATGERAQEVADLKRARSLPGRHNWQNAAAAY
HCCCEEEEEECHHHHHHCCCCHHHCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
AAARGLGISGEEAAQHLMTFPGLAHRMETVAVLGKVRFVNDSKATNADAARQAMSSYPKF
HHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCE
YWIAGGLPKTGGIDGLIDLFPRIEKAYLVGEAAPAFADVLRGKAPAVESGTIEAAVRQAY
EEEECCCCCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHH
ADARAAGQEAIVLLSPACASFDQFADFEERGEAFRAAVQKLADGQADAA
HHHHHCCCCEEEEECCCHHCHHHHCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA