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The map label for this gene is rsuA [H]

Identifier: 197105762

GI number: 197105762

Start: 2605997

End: 2606725

Strand: Reverse

Name: rsuA [H]

Synonym: PHZ_c2300

Alternate gene names: 197105762

Gene position: 2606725-2605997 (Counterclockwise)

Preceding gene: 197105763

Following gene: 197105757

Centisome position: 65.23

GC content: 72.7

Gene sequence:

>729_bases
ATGAGCGGCTCGGTCCGCCTCGACAAGCTGCTGGCCAACATGGGCTATGGCTCGCGCCGGGAGGTGCAGGCGCTGGCCCG
CGCCGGGGCCATCACCCTGGACGGCGCGCCCGTGCCCAAGGCCGACACGCGGATCGCGCTGACCCCGGATCTGCCGGCGC
GGATGCAAGTGGACGGACGGCCGCTGGATCCGCTGCCCGGCATGGCGCTGATGCTGCACAAGCCCACGGGCGTGACGTGC
TCGCACAAGGAGACGGGCGAGCTGGTCTATAGCCTCCTGCCGTCGCGCTGGCGGATGCGCGAGCCGCCGCTCTCCACGGT
GGGCCGGCTGGACAAGGAGACGTCCGGCCTGCTGCTGCTGACCGACGACGGCGACCTGCTGCACCGGATCATTTCGCCCA
GGCGGCACGTCGCCAAGCGCTACGTGGCCACCCTGGCCCGGCCGCTGCGCGGCGACGAGGGGGCCATCTTCGCCGCGGGC
GAGCTGATGCTGGAGGGCGAAGACAAGCCGCTGGCGCCGGCGGTGCTGGAGCCCCTGTCGCCGACCCTGGCGCGGCTGAC
CATCACCGAGGGTCGCTACCACCAGGTGCGCCGGATGTTCGCCGCCGTCGGCAACCATGTGGAGGCGCTGCACCGGGACC
GCATCGGCGGCCTGGACCTTCCTGCCGACCTGGCCCCCGGTCGCTATCGGCTGATGGGGGCCGAGGACCTGGCGGCGATC
TTCGCCTAG

Upstream 100 bases:

>100_bases
CCAACCGGCACCTGCCGTACGAGGGCGTCCTGGGCGACCTGTTCGGCAAGGCCGCGCTGAAGGCCGAAGGTGGAGGCTTC
AAGGTGTACGAGGCGCGGAA

Downstream 100 bases:

>100_bases
GCGCGCTTGCCGTCCAGCCGGTCCACGATCCGGCCCACGCGGCCGTGCGACCAGGCGAGGCCCTGGTAGACGGCCAGGCT
CGCCCTGGCCCAGCGCGTGT

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSGSVRLDKLLANMGYGSRREVQALARAGAITLDGAPVPKADTRIALTPDLPARMQVDGRPLDPLPGMALMLHKPTGVTC
SHKETGELVYSLLPSRWRMREPPLSTVGRLDKETSGLLLLTDDGDLLHRIISPRRHVAKRYVATLARPLRGDEGAIFAAG
ELMLEGEDKPLAPAVLEPLSPTLARLTITEGRYHQVRRMFAAVGNHVEALHRDRIGGLDLPADLAPGRYRLMGAEDLAAI
FA

Sequences:

>Translated_242_residues
MSGSVRLDKLLANMGYGSRREVQALARAGAITLDGAPVPKADTRIALTPDLPARMQVDGRPLDPLPGMALMLHKPTGVTC
SHKETGELVYSLLPSRWRMREPPLSTVGRLDKETSGLLLLTDDGDLLHRIISPRRHVAKRYVATLARPLRGDEGAIFAAG
ELMLEGEDKPLAPAVLEPLSPTLARLTITEGRYHQVRRMFAAVGNHVEALHRDRIGGLDLPADLAPGRYRLMGAEDLAAI
FA
>Mature_241_residues
SGSVRLDKLLANMGYGSRREVQALARAGAITLDGAPVPKADTRIALTPDLPARMQVDGRPLDPLPGMALMLHKPTGVTCS
HKETGELVYSLLPSRWRMREPPLSTVGRLDKETSGLLLLTDDGDLLHRIISPRRHVAKRYVATLARPLRGDEGAIFAAGE
LMLEGEDKPLAPAVLEPLSPTLARLTITEGRYHQVRRMFAAVGNHVEALHRDRIGGLDLPADLAPGRYRLMGAEDLAAIF
A

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=240, Percent_Identity=44.1666666666667, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1787524, Length=237, Percent_Identity=32.4894514767932, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=29.3388429752066, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26297; Mature: 26166

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSVRLDKLLANMGYGSRREVQALARAGAITLDGAPVPKADTRIALTPDLPARMQVDGR
CCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCEEEECCC
PLDPLPGMALMLHKPTGVTCSHKETGELVYSLLPSRWRMREPPLSTVGRLDKETSGLLLL
CCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHCCCCCCCEEEE
TDDGDLLHRIISPRRHVAKRYVATLARPLRGDEGAIFAAGELMLEGEDKPLAPAVLEPLS
ECCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEECEEEEECCCCCCCHHHHHCCC
PTLARLTITEGRYHQVRRMFAAVGNHVEALHRDRIGGLDLPADLAPGRYRLMGAEDLAAI
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHH
FA
CC
>Mature Secondary Structure 
SGSVRLDKLLANMGYGSRREVQALARAGAITLDGAPVPKADTRIALTPDLPARMQVDGR
CCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCCCCCCCEEEECCCCCCEEEECCC
PLDPLPGMALMLHKPTGVTCSHKETGELVYSLLPSRWRMREPPLSTVGRLDKETSGLLLL
CCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHCCCCCCCEEEE
TDDGDLLHRIISPRRHVAKRYVATLARPLRGDEGAIFAAGELMLEGEDKPLAPAVLEPLS
ECCHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEECEEEEECCCCCCCHHHHHCCC
PTLARLTITEGRYHQVRRMFAAVGNHVEALHRDRIGGLDLPADLAPGRYRLMGAEDLAAI
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECHHHHHHH
FA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]