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The map label for this gene is bcr [H]

Identifier: 197105738

GI number: 197105738

Start: 2578186

End: 2579400

Strand: Reverse

Name: bcr [H]

Synonym: PHZ_c2276

Alternate gene names: 197105738

Gene position: 2579400-2578186 (Counterclockwise)

Preceding gene: 197105742

Following gene: 197105734

Centisome position: 64.55

GC content: 73.5

Gene sequence:

>1215_bases
GTGACGTACGCCAACACCACATCGCCGGACCGCACGCCCTGGGGGCTGGTGGTCCTGCTCGGGGCGCTGACGGCCATGGG
GCCGCTCGCCATCGACATGTACCTGCCGAGCCTGCCGGCCATCGGCGAGGATCTGCGCGCCACCTCGGGCCAGACCCAGG
GCACCGTCGCCGCCTTCCTCGCGGGCATGGCGATCGGCCAGTTCTTCTACGGCCCGGCCTCGGACCGGTTCGGGCGCAAG
CCGCCGATCCTGGTGGGCGTCCTGATCTTCATCCTGGCCTCGGCGGGCTGCGCGCTCGCCGCCTCGCCGGAGCAGCTGCT
GGCCGGGCGGTTCGTCCAGGCGCTGGGCGCCTGCGCGGGCGGTGTGGTCTCGCGCGCGGTGGTGCGCGACCGCTTCAACC
ACACCGAGACGGCGCGGATGCTCAGCCTGCTGATGCTGATCATGGGCCTGGCGCCGATCCTCGCGCCGCTGCTGGGCGGC
GCCCTGCTCACCGTGGGCGGCTGGCGGCTGAACTTCTGGTTCATGGCCGCCTTCGGCCTGGCGATCGGCGCGGCGACGAT
GCTTCGCCTGCAGGAGTCCCGCAGCGAGGAGACGACGCGGCAGGCGCAGTCCGAGAGCCCGCTGCAGGCCTACGCCGCCC
TGCTGCGGCAGCCGCGGCTGGTGGGCTACGCCCTGGCCGGGGCGCTGAACGGGGCGACGCTGTTCACCTACATCTCGGCC
TCGCCCGAGCTGCTGATCGAGACCTACGGCGTGCCGCCCTCGGCGTTCGGCTGGCTGTTCGGCCTGAACGCCGCCGGGGT
GATCGGCGCGAACCAGGTGAACCGCTGGCTGCTGCGCACCCGCACGCCCGACCAGGTCCTGGCGCGGGCGAGCACGGTGG
CGGTGGCGTTCGCCGTCGCCCTGGCGCTGGCGGCGGTCACCGGGATCGGAGAGCGCTGGAGCGTCCTGCCGCTGCTGTTC
CTGCTGCTGGCCAGCTACGGCTTCATGCAGGGCAACACCATGGCCGGCGCCCTCAGCGTCGATCCGCGGCGGGCCGGCTC
GATCTCGGCCCTGCTGGGCGGGGTCTCGTTCGGCATGGGCGCGCTGGCCTCCAGCCTGGCCGGCGCGTTCCACGACGGCA
CGCCCCGGCCCATGGCGCTGGTGATGCTGGTCGCGCTTGTCGGTTCGGCCGTGGCGCTGAGGAAGCTGGCCCTGAAGCCC
GGCCCTCAGGTCTGA

Upstream 100 bases:

>100_bases
GCGCAGACACGGCTTTTCCCCCTCATTTTCGGCGTTGCGGCCAGCATCGCCGCAAAACCGCTTTCCCGGAAGCGGGCGGC
GCCAGTAGGGCACGCCTGTC

Downstream 100 bases:

>100_bases
CAGCTCTCAGGTCTGGCGGCCTTCAGGTTTGGCGGCGCGCCACCGCCCAGGCGAGGGCGGCCCATCCGGCCATGAAGCTG
ACGCCGCCGATCGGCGTGAT

Product: drug resistance transporter, Bcr/CflA subfamily

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MTYANTTSPDRTPWGLVVLLGALTAMGPLAIDMYLPSLPAIGEDLRATSGQTQGTVAAFLAGMAIGQFFYGPASDRFGRK
PPILVGVLIFILASAGCALAASPEQLLAGRFVQALGACAGGVVSRAVVRDRFNHTETARMLSLLMLIMGLAPILAPLLGG
ALLTVGGWRLNFWFMAAFGLAIGAATMLRLQESRSEETTRQAQSESPLQAYAALLRQPRLVGYALAGALNGATLFTYISA
SPELLIETYGVPPSAFGWLFGLNAAGVIGANQVNRWLLRTRTPDQVLARASTVAVAFAVALALAAVTGIGERWSVLPLLF
LLLASYGFMQGNTMAGALSVDPRRAGSISALLGGVSFGMGALASSLAGAFHDGTPRPMALVMLVALVGSAVALRKLALKP
GPQV

Sequences:

>Translated_404_residues
MTYANTTSPDRTPWGLVVLLGALTAMGPLAIDMYLPSLPAIGEDLRATSGQTQGTVAAFLAGMAIGQFFYGPASDRFGRK
PPILVGVLIFILASAGCALAASPEQLLAGRFVQALGACAGGVVSRAVVRDRFNHTETARMLSLLMLIMGLAPILAPLLGG
ALLTVGGWRLNFWFMAAFGLAIGAATMLRLQESRSEETTRQAQSESPLQAYAALLRQPRLVGYALAGALNGATLFTYISA
SPELLIETYGVPPSAFGWLFGLNAAGVIGANQVNRWLLRTRTPDQVLARASTVAVAFAVALALAAVTGIGERWSVLPLLF
LLLASYGFMQGNTMAGALSVDPRRAGSISALLGGVSFGMGALASSLAGAFHDGTPRPMALVMLVALVGSAVALRKLALKP
GPQV
>Mature_403_residues
TYANTTSPDRTPWGLVVLLGALTAMGPLAIDMYLPSLPAIGEDLRATSGQTQGTVAAFLAGMAIGQFFYGPASDRFGRKP
PILVGVLIFILASAGCALAASPEQLLAGRFVQALGACAGGVVSRAVVRDRFNHTETARMLSLLMLIMGLAPILAPLLGGA
LLTVGGWRLNFWFMAAFGLAIGAATMLRLQESRSEETTRQAQSESPLQAYAALLRQPRLVGYALAGALNGATLFTYISAS
PELLIETYGVPPSAFGWLFGLNAAGVIGANQVNRWLLRTRTPDQVLARASTVAVAFAVALALAAVTGIGERWSVLPLLFL
LLASYGFMQGNTMAGALSVDPRRAGSISALLGGVSFGMGALASSLAGAFHDGTPRPMALVMLVALVGSAVALRKLALKPG
PQV

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=360, Percent_Identity=33.0555555555556, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1790146, Length=340, Percent_Identity=33.5294117647059, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI48994889, Length=242, Percent_Identity=35.5371900826446, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1787065, Length=229, Percent_Identity=30.1310043668122, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082312, Length=174, Percent_Identity=33.9080459770115, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790794, Length=266, Percent_Identity=26.3157894736842, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789207, Length=133, Percent_Identity=30.8270676691729, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=163, Percent_Identity=28.2208588957055, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41943; Mature: 41812

Theoretical pI: Translated: 10.35; Mature: 10.35

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYANTTSPDRTPWGLVVLLGALTAMGPLAIDMYLPSLPAIGEDLRATSGQTQGTVAAFL
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHH
AGMAIGQFFYGPASDRFGRKPPILVGVLIFILASAGCALAASPEQLLAGRFVQALGACAG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
GVVSRAVVRDRFNHTETARMLSLLMLIMGLAPILAPLLGGALLTVGGWRLNFWFMAAFGL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHH
AIGAATMLRLQESRSEETTRQAQSESPLQAYAALLRQPRLVGYALAGALNGATLFTYISA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECC
SPELLIETYGVPPSAFGWLFGLNAAGVIGANQVNRWLLRTRTPDQVLARASTVAVAFAVA
CHHEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LALAAVTGIGERWSVLPLLFLLLASYGFMQGNTMAGALSVDPRRAGSISALLGGVSFGMG
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
ALASSLAGAFHDGTPRPMALVMLVALVGSAVALRKLALKPGPQV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TYANTTSPDRTPWGLVVLLGALTAMGPLAIDMYLPSLPAIGEDLRATSGQTQGTVAAFL
CCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHH
AGMAIGQFFYGPASDRFGRKPPILVGVLIFILASAGCALAASPEQLLAGRFVQALGACAG
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
GVVSRAVVRDRFNHTETARMLSLLMLIMGLAPILAPLLGGALLTVGGWRLNFWFMAAFGL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHH
AIGAATMLRLQESRSEETTRQAQSESPLQAYAALLRQPRLVGYALAGALNGATLFTYISA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEECC
SPELLIETYGVPPSAFGWLFGLNAAGVIGANQVNRWLLRTRTPDQVLARASTVAVAFAVA
CHHEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LALAAVTGIGERWSVLPLLFLLLASYGFMQGNTMAGALSVDPRRAGSISALLGGVSFGMG
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
ALASSLAGAFHDGTPRPMALVMLVALVGSAVALRKLALKPGPQV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]