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The map label for this gene is ogt [H]

Identifier: 197105717

GI number: 197105717

Start: 2555012

End: 2555545

Strand: Reverse

Name: ogt [H]

Synonym: PHZ_c2255

Alternate gene names: 197105717

Gene position: 2555545-2555012 (Counterclockwise)

Preceding gene: 197105718

Following gene: 197105716

Centisome position: 63.95

GC content: 74.72

Gene sequence:

>534_bases
ATGATCGTGCAGCCGCCTGAAACCCTGACCCTCGACCGCATCGCGACCCCGATCGGCACGGCCCTCGTGGTCACCGACGA
GGCGGGCGTCCTGCGGGCCTTCAACTGGACCGACTACGAGGACCGGATGCTGGCCTGGGTCGCCCGCCGCTATCCGAAGG
CCGCGCTCGTCGAAGGGCGCAGCCCCGCGCGGCCGGCGCTGGAGGCCTATTTCGCCGGGGACCTCGGGGCTCTGTCGGCC
GTGCCCTGGGACGGCGCGGGCACGGCGTTCCAGCGGAAGGTGTGGGCCGCGCTCTGCGCCATCCCGGTCGGGGAGACCTG
GAGCTACGCGCGCCTGGCGCAGGCGATCGGCCGGCCGACGGCCGTACGCGCCGTGGGGCTCGCCAACGGCTCCAATCCCG
TGGCGCTGGTCGTGCCCTGCCACCGGGTGATCGGCTCGGACGGCTCGCTCACCGGCTACGGCGGCGGGCTGCCGCGCAAG
CGCTGGCTGCTGGCCCACGAGGGCGCGAGCTTCCGGGATCGCCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
CTCGCCCGCAGCGCCGCCTGGCGGCCCTGGCGCGCCTACGCCGCCCAACATCTCTGGGCCCACGACGCGGCCCAACCCGA
CCTCAAGAGGACCAAGACCG

Downstream 100 bases:

>100_bases
TGGCGCCGGCGCAACATTGCCGAAATTTAAGGTCCGCGCCGCTCGCGGCGAAGCCGTCCGCGTCGTATAACGCCGCTCGG
CGGAGTCGCGCAGGAATCGG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIVQPPETLTLDRIATPIGTALVVTDEAGVLRAFNWTDYEDRMLAWVARRYPKAALVEGRSPARPALEAYFAGDLGALSA
VPWDGAGTAFQRKVWAALCAIPVGETWSYARLAQAIGRPTAVRAVGLANGSNPVALVVPCHRVIGSDGSLTGYGGGLPRK
RWLLAHEGASFRDRLAA

Sequences:

>Translated_177_residues
MIVQPPETLTLDRIATPIGTALVVTDEAGVLRAFNWTDYEDRMLAWVARRYPKAALVEGRSPARPALEAYFAGDLGALSA
VPWDGAGTAFQRKVWAALCAIPVGETWSYARLAQAIGRPTAVRAVGLANGSNPVALVVPCHRVIGSDGSLTGYGGGLPRK
RWLLAHEGASFRDRLAA
>Mature_177_residues
MIVQPPETLTLDRIATPIGTALVVTDEAGVLRAFNWTDYEDRMLAWVARRYPKAALVEGRSPARPALEAYFAGDLGALSA
VPWDGAGTAFQRKVWAALCAIPVGETWSYARLAQAIGRPTAVRAVGLANGSNPVALVVPCHRVIGSDGSLTGYGGGLPRK
RWLLAHEGASFRDRLAA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=81, Percent_Identity=50.6172839506173, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1787596, Length=165, Percent_Identity=47.8787878787879, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1788542, Length=159, Percent_Identity=37.7358490566038, Blast_Score=102, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=54.1176470588235, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=54.1176470588235, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=92, Percent_Identity=46.7391304347826, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137554, Length=180, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18866; Mature: 18866

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00037 MYB_1 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVQPPETLTLDRIATPIGTALVVTDEAGVLRAFNWTDYEDRMLAWVARRYPKAALVEGR
CCCCCCCCEEHHHHHCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCC
SPARPALEAYFAGDLGALSAVPWDGAGTAFQRKVWAALCAIPVGETWSYARLAQAIGRPT
CCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
AVRAVGLANGSNPVALVVPCHRVIGSDGSLTGYGGGLPRKRWLLAHEGASFRDRLAA
CEEEEEECCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCC
>Mature Secondary Structure
MIVQPPETLTLDRIATPIGTALVVTDEAGVLRAFNWTDYEDRMLAWVARRYPKAALVEGR
CCCCCCCCEEHHHHHCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCEEECCC
SPARPALEAYFAGDLGALSAVPWDGAGTAFQRKVWAALCAIPVGETWSYARLAQAIGRPT
CCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
AVRAVGLANGSNPVALVVPCHRVIGSDGSLTGYGGGLPRKRWLLAHEGASFRDRLAA
CEEEEEECCCCCCEEEEEECHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]