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The map label for this gene is gcvA [H]

Identifier: 197105467

GI number: 197105467

Start: 2241229

End: 2242128

Strand: Reverse

Name: gcvA [H]

Synonym: PHZ_c2004

Alternate gene names: 197105467

Gene position: 2242128-2241229 (Counterclockwise)

Preceding gene: 197105468

Following gene: 197105466

Centisome position: 56.11

GC content: 73.33

Gene sequence:

>900_bases
ATGCCGCCGTTCGCTGCGCTGCGGGCGTTTGAAGCCGTGGGCGCGCTGGGCGGGATCCGCCGGGCGGCGCAGGAACTCGG
CGTCGACCACGCAGCGGTCAGCCGCCACATCCGCTTCCTGGAGGACTGGGCCGGCGTCCCGCTTATCGACCGGCCGCAGG
GCGGGCGGCTCACCCCCGAGGGGCAGCGCTTCCATCAGCGCATCGCCGCGGCTCTGGAGGAGATCGTCAGCGCGAGCGCC
GAACTGACCCGGCGCGGCCACGAGCGCCGGCTCCTCGTCTGGTGCGTCCCCGGCTTCGCGTACCGCTGGCTGAACGCTCG
GCTAGCGGACTTCTCCGCCTCGCACCCGGACTTCGACCTCGAAATCCGACCCACCGACCACGCGCCGGACCTCGCACGGC
ATGAGGCCGACGCCGACATCCGGTACGTGCACGACTACGATCCGCCGCAGCCGGCCGATGTCGAGACGATGGAGATCGCA
AGGCCCCCGGTGATCCCCGTGGGCAGCCCCGACCTGGTCGCCAGCCTCGGCCCGCCGGTGTCGCCGCAGCGGTTCCTGGA
CGGGCCGTTGCTCCACGAGGAGGGAGACGGCGAGTGGCGGGCCTGGCTCGCGGGCAACGGGGTGGAGGTTCCCGACCGCC
TTCCCGGCCCTCGCCTTTGGCACGCGCACCTGACGATCGATGCGGCGCGCCGAGGGCAGGGGATCGCCCTGACGAACCCG
TTCCTCGCCGGGGAGGACCTGGCGGCGGGCAATCTCCGCATCGTGCCGCTGGTCGAGGGCGCCGCTGACGTCCGGCTGGG
CGCCTACGTCTTCCGCACCCGACGCGACCGCTGGCGGACGCCGGCGGTGATCGCGTTCCGCCGCTGGCTCGAACGAGAAG
CCGCGAAGACCCCGGCCTGA

Upstream 100 bases:

>100_bases
CAGAGGGGCCGACGGGGCCGCCGCGTAGGACAGAGCCGGCATGCCGCGCGAGAAGCAGAGAGCTGGGACCTCGAGCCTGC
GCACCCTGGGCGCGCGGGCC

Downstream 100 bases:

>100_bases
CGGTCGCCGCTCCTTACGAATCGCGGGAAGCGTGCCGCCCACCCCAGACGGAGCGCTCGTTTGCCCAATCCCGTGCTGCT
GTTCGCCTGCCACGATGTTC

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MPPFAALRAFEAVGALGGIRRAAQELGVDHAAVSRHIRFLEDWAGVPLIDRPQGGRLTPEGQRFHQRIAAALEEIVSASA
ELTRRGHERRLLVWCVPGFAYRWLNARLADFSASHPDFDLEIRPTDHAPDLARHEADADIRYVHDYDPPQPADVETMEIA
RPPVIPVGSPDLVASLGPPVSPQRFLDGPLLHEEGDGEWRAWLAGNGVEVPDRLPGPRLWHAHLTIDAARRGQGIALTNP
FLAGEDLAAGNLRIVPLVEGAADVRLGAYVFRTRRDRWRTPAVIAFRRWLEREAAKTPA

Sequences:

>Translated_299_residues
MPPFAALRAFEAVGALGGIRRAAQELGVDHAAVSRHIRFLEDWAGVPLIDRPQGGRLTPEGQRFHQRIAAALEEIVSASA
ELTRRGHERRLLVWCVPGFAYRWLNARLADFSASHPDFDLEIRPTDHAPDLARHEADADIRYVHDYDPPQPADVETMEIA
RPPVIPVGSPDLVASLGPPVSPQRFLDGPLLHEEGDGEWRAWLAGNGVEVPDRLPGPRLWHAHLTIDAARRGQGIALTNP
FLAGEDLAAGNLRIVPLVEGAADVRLGAYVFRTRRDRWRTPAVIAFRRWLEREAAKTPA
>Mature_298_residues
PPFAALRAFEAVGALGGIRRAAQELGVDHAAVSRHIRFLEDWAGVPLIDRPQGGRLTPEGQRFHQRIAAALEEIVSASAE
LTRRGHERRLLVWCVPGFAYRWLNARLADFSASHPDFDLEIRPTDHAPDLARHEADADIRYVHDYDPPQPADVETMEIAR
PPVIPVGSPDLVASLGPPVSPQRFLDGPLLHEEGDGEWRAWLAGNGVEVPDRLPGPRLWHAHLTIDAARRGQGIALTNPF
LAGEDLAAGNLRIVPLVEGAADVRLGAYVFRTRRDRWRTPAVIAFRRWLEREAAKTPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=29.7297297297297, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=28.9115646258503, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788706, Length=304, Percent_Identity=24.0131578947368, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33104; Mature: 32973

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPFAALRAFEAVGALGGIRRAAQELGVDHAAVSRHIRFLEDWAGVPLIDRPQGGRLTPE
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GQRFHQRIAAALEEIVSASAELTRRGHERRLLVWCVPGFAYRWLNARLADFSASHPDFDL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCEE
EIRPTDHAPDLARHEADADIRYVHDYDPPQPADVETMEIARPPVIPVGSPDLVASLGPPV
EEECCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCEECCCCCHHHHHCCCCC
SPQRFLDGPLLHEEGDGEWRAWLAGNGVEVPDRLPGPRLWHAHLTIDAARRGQGIALTNP
CHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEEEHHHCCCCEEEECC
FLAGEDLAAGNLRIVPLVEGAADVRLGAYVFRTRRDRWRTPAVIAFRRWLEREAAKTPA
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PPFAALRAFEAVGALGGIRRAAQELGVDHAAVSRHIRFLEDWAGVPLIDRPQGGRLTPE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
GQRFHQRIAAALEEIVSASAELTRRGHERRLLVWCVPGFAYRWLNARLADFSASHPDFDL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCEE
EIRPTDHAPDLARHEADADIRYVHDYDPPQPADVETMEIARPPVIPVGSPDLVASLGPPV
EEECCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCHHHHCCCCEECCCCCHHHHHCCCCC
SPQRFLDGPLLHEEGDGEWRAWLAGNGVEVPDRLPGPRLWHAHLTIDAARRGQGIALTNP
CHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEEEEHHHCCCCEEEECC
FLAGEDLAAGNLRIVPLVEGAADVRLGAYVFRTRRDRWRTPAVIAFRRWLEREAAKTPA
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]