Click here to switch to the map view.

The map label for this gene is trmFO

Identifier: 197105412

GI number: 197105412

Start: 2183847

End: 2185247

Strand: Direct

Name: trmFO

Synonym: PHZ_c1949

Alternate gene names: 197105412

Gene position: 2183847-2185247 (Clockwise)

Preceding gene: 197105410

Following gene: 197105419

Centisome position: 54.65

GC content: 72.16

Gene sequence:

>1401_bases
ATGTCGATGAACCCTATCCATGTCGTGGGCGGCGGCCTGGCCGGCTCGGAAGCCGCCTGGCAGATCGCCGAGGCCGGCGT
GCCGGTCGTGCTGCACGAGATGCGCCCGGTGCGCCGCACCGACGCGCACCACACCGACCGCCTCGCCGAGCTGGTCTGCT
CGAACTCGTTCCGGGCCGACGACTGGACCGGCAACGCGGTCGGCCTGCTGCACGCCGAGATGCGCCGGCTGGGCTCGATC
ATCATGTCGGCGGGGGACGCCCACCAGGTGCCGGCCGGCGGCGCGCTGGCCGTGGACCGCGAGGGCTTCTCCCAGGCCGT
GACCGAGCGGCTGGAGGCCCATCCGCTGGTGACCATCGCCCGCGAGGAGATCGCCGGCCTGCCGCCGCAGGACTGGGACA
GCGTGATCCTGGCCACCGGCCCCCTCACCTCGCCCGCCCTGGCCGACGCGGTGCTGAAGCTGACCGGCGAGGACCAGCTG
TCGTTCTTCGACGCCATCGCCCCGATCGTGCACTTCGAGTCCATCGACATGGACGTCGCCTGGCGCCAGTCGCGCTACGA
CAAGGCCGGGCCCGGCGGCGATGCGGCGGCCTACATCAACTGCCCGATGACCGAGGCGCAGTACGAGGCGTTCATCGACG
CCCTGCTGGCCGCCCCGAAGGCCGAGTTCAAGGAGTGGGAGCACGTGCCCTACTTCGACGGCTGCCTGCCGATCGAGGTG
ATGGCCGAGCGCGGGCGCGAGACCCTGCGCCACGGGCCGATGAAGCCGGTCGGCCTGACCAACGCCCACAGGCCCGACGA
GAAGCCCCACGCCGTCGTCCAGCTGCGCCAGGACAACGCCCTGGGGACCCTGTGGAACATGGTGGGCTTCCAGACCAAGC
TGAAGCACGGCGCCCAGACCGAGGTGTTCCGGATGATCCCGGGCCTCGAAAAGGCGGTGTTCGCCAGGCTCGGCGGCCTG
CACCGCAACACCTTCATCAACAGCCCCCGGCTGCTGGACGGCGTCCTGCGCCTGAAGGCCCAGCCGCGCCTGCGCTTCGC
GGGCCAGGTGACGGGCGTGGAGGGCTATGTGGAGAGCGCCGCGGTAGGCCTGCTGGCCGGGCGCTTCGCCGCCGCCGAAC
GCCTGGGCCGCCCCGCCGTCGCACCGCCGCCGACCACGGCCCTGGGCGGACTGATCGGGCACATCACCGGCGGGCATCTG
GAGGGCGGGAGCGGTTCGTTCCAGCCGATGAACATCAACTACGGCCTGATCCCGCCCATCGCTCCGCCCAAGCGCGACGC
GGAGGGCCGGCGCCTGGGTGCCAAGGAGAAGACCCGAATCAAGAAGCGGCTGGTGGGCGAGCGGGCCCTGGCCGACCTCG
AGGCCTGGCTGAGCGGGGCGCAGGCGGTCGCGGCCGAGTAG

Upstream 100 bases:

>100_bases
GATTCGGGCCGCCCCTTCAGCCGCCCACGTTGCCGCGCCGAGGATCAAGCGCGCAAGGCCCCGCTCGCGATCCCGGCCGT
TCGTTGCTAGTGAACCGGCC

Downstream 100 bases:

>100_bases
CGCCCGCTCCCGATCCGGGGGAAGTCAGATCCGGCCGCGGGCGACCGCCAGGATCAGGCGCAGCCGGTCCCCGACCGGCG
AGCGGCGGCGGCCGATCAGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSMNPIHVVGGGLAGSEAAWQIAEAGVPVVLHEMRPVRRTDAHHTDRLAELVCSNSFRADDWTGNAVGLLHAEMRRLGSI
IMSAGDAHQVPAGGALAVDREGFSQAVTERLEAHPLVTIAREEIAGLPPQDWDSVILATGPLTSPALADAVLKLTGEDQL
SFFDAIAPIVHFESIDMDVAWRQSRYDKAGPGGDAAAYINCPMTEAQYEAFIDALLAAPKAEFKEWEHVPYFDGCLPIEV
MAERGRETLRHGPMKPVGLTNAHRPDEKPHAVVQLRQDNALGTLWNMVGFQTKLKHGAQTEVFRMIPGLEKAVFARLGGL
HRNTFINSPRLLDGVLRLKAQPRLRFAGQVTGVEGYVESAAVGLLAGRFAAAERLGRPAVAPPPTTALGGLIGHITGGHL
EGGSGSFQPMNINYGLIPPIAPPKRDAEGRRLGAKEKTRIKKRLVGERALADLEAWLSGAQAVAAE

Sequences:

>Translated_466_residues
MSMNPIHVVGGGLAGSEAAWQIAEAGVPVVLHEMRPVRRTDAHHTDRLAELVCSNSFRADDWTGNAVGLLHAEMRRLGSI
IMSAGDAHQVPAGGALAVDREGFSQAVTERLEAHPLVTIAREEIAGLPPQDWDSVILATGPLTSPALADAVLKLTGEDQL
SFFDAIAPIVHFESIDMDVAWRQSRYDKAGPGGDAAAYINCPMTEAQYEAFIDALLAAPKAEFKEWEHVPYFDGCLPIEV
MAERGRETLRHGPMKPVGLTNAHRPDEKPHAVVQLRQDNALGTLWNMVGFQTKLKHGAQTEVFRMIPGLEKAVFARLGGL
HRNTFINSPRLLDGVLRLKAQPRLRFAGQVTGVEGYVESAAVGLLAGRFAAAERLGRPAVAPPPTTALGGLIGHITGGHL
EGGSGSFQPMNINYGLIPPIAPPKRDAEGRRLGAKEKTRIKKRLVGERALADLEAWLSGAQAVAAE
>Mature_465_residues
SMNPIHVVGGGLAGSEAAWQIAEAGVPVVLHEMRPVRRTDAHHTDRLAELVCSNSFRADDWTGNAVGLLHAEMRRLGSII
MSAGDAHQVPAGGALAVDREGFSQAVTERLEAHPLVTIAREEIAGLPPQDWDSVILATGPLTSPALADAVLKLTGEDQLS
FFDAIAPIVHFESIDMDVAWRQSRYDKAGPGGDAAAYINCPMTEAQYEAFIDALLAAPKAEFKEWEHVPYFDGCLPIEVM
AERGRETLRHGPMKPVGLTNAHRPDEKPHAVVQLRQDNALGTLWNMVGFQTKLKHGAQTEVFRMIPGLEKAVFARLGGLH
RNTFINSPRLLDGVLRLKAQPRLRFAGQVTGVEGYVESAAVGLLAGRFAAAERLGRPAVAPPPTTALGGLIGHITGGHLE
GGSGSFQPMNINYGLIPPIAPPKRDAEGRRLGAKEKTRIKKRLVGERALADLEAWLSGAQAVAAE

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_PHEZH (B4RDH0)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130789.1
- GeneID:   6791465
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1949
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 50059; Mature: 49928

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMNPIHVVGGGLAGSEAAWQIAEAGVPVVLHEMRPVRRTDAHHTDRLAELVCSNSFRAD
CCCCCEEEEECCCCCCHHHHHHHHCCCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCC
DWTGNAVGLLHAEMRRLGSIIMSAGDAHQVPAGGALAVDREGFSQAVTERLEAHPLVTIA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCHHEEE
REEIAGLPPQDWDSVILATGPLTSPALADAVLKLTGEDQLSFFDAIAPIVHFESIDMDVA
HHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHH
WRQSRYDKAGPGGDAAAYINCPMTEAQYEAFIDALLAAPKAEFKEWEHVPYFDGCLPIEV
HHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHH
MAERGRETLRHGPMKPVGLTNAHRPDEKPHAVVQLRQDNALGTLWNMVGFQTKLKHGAQT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHH
EVFRMIPGLEKAVFARLGGLHRNTFINSPRLLDGVLRLKAQPRLRFAGQVTGVEGYVESA
HHHHHCCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCEEECCEECCHHHHHHHH
AVGLLAGRFAAAERLGRPAVAPPPTTALGGLIGHITGGHLEGGSGSFQPMNINYGLIPPI
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCC
APPKRDAEGRRLGAKEKTRIKKRLVGERALADLEAWLSGAQAVAAE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SMNPIHVVGGGLAGSEAAWQIAEAGVPVVLHEMRPVRRTDAHHTDRLAELVCSNSFRAD
CCCCEEEEECCCCCCHHHHHHHHCCCCEEECCCCCHHHCCCHHHHHHHHHHHCCCCCCC
DWTGNAVGLLHAEMRRLGSIIMSAGDAHQVPAGGALAVDREGFSQAVTERLEAHPLVTIA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCHHEEE
REEIAGLPPQDWDSVILATGPLTSPALADAVLKLTGEDQLSFFDAIAPIVHFESIDMDVA
HHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHH
WRQSRYDKAGPGGDAAAYINCPMTEAQYEAFIDALLAAPKAEFKEWEHVPYFDGCLPIEV
HHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCHHHH
MAERGRETLRHGPMKPVGLTNAHRPDEKPHAVVQLRQDNALGTLWNMVGFQTKLKHGAQT
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCHH
EVFRMIPGLEKAVFARLGGLHRNTFINSPRLLDGVLRLKAQPRLRFAGQVTGVEGYVESA
HHHHHCCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHCCCCCEEECCEECCHHHHHHHH
AVGLLAGRFAAAERLGRPAVAPPPTTALGGLIGHITGGHLEGGSGSFQPMNINYGLIPPI
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCC
APPKRDAEGRRLGAKEKTRIKKRLVGERALADLEAWLSGAQAVAAE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA