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The map label for this gene is rne [H]

Identifier: 197105305

GI number: 197105305

Start: 2064179

End: 2066869

Strand: Direct

Name: rne [H]

Synonym: PHZ_c1842

Alternate gene names: 197105305

Gene position: 2064179-2066869 (Clockwise)

Preceding gene: 197105301

Following gene: 197105306

Centisome position: 51.65

GC content: 72.35

Gene sequence:

>2691_bases
ATGTCCAAGACCATGTTGATCGACGCAGCCCACGCGGAAGAGACGCGCGTGGTGGTCGTCGACGGGAACCGCGTTGAAGA
GTTCGATTTCGAAAGCCAGAACCGGAAGCAGCTTCGAGGCAACATCTATCTCGCCAAGGTGACGCGCGTTGAGCCGTCGC
TGCAGGCGGCCTTCGTCGAATACGGCGGCAACCGCCACGGCTTCCTGGCCTTCAACGAAATCCACCCCGACTACTACCAG
ATCCCGGTCGCCGACCGCGAAGCGCTGCTCCGCGAGGAGGCCGAGGAGGACGACGACGACCGTCACGAGCCCCGCGCCCG
GGCGCCCGAGGACGAGGACGAGGTCGACCTGTCCGGCTCCGCTGACGACGAGGACGACGTCGTCGAGGAAGAGCTGGCGC
GCCGCCGCCGCCGGCTGATGCGCCGCTACAAGATCCAGGAAGTGATCCGCCGCCGGCAGATCATGCTGGTCCAGGTCGTC
AAGGAAGAGCGCGGCAACAAGGGCGCGGCGCTGACCACCTACCTGTCGCTGGCCGGCCGCTATGGCGTACTGATGCCGAA
CACCGCCCGCGGCGGCGGCATCAGCCGCAAGATCACCACGGCGACCGACCGCAAGCGCCTGAAGAGCATCGTCCAGAGCC
TGGACGTGCCCGCCGGCATGGGCCTCATCGTCCGCACGGCGGGCGCCAAGCGCACCAAGGCCGAGATCAAGCGCGACTAC
GACTACCTGCTGCGCCTGTGGGAGAACATCCGCGAGACGACGCTGCACTCGATCGCGCCCGCCCTCATCTACGAGGAAGA
GGACCTCGTGAAGCGGGCGATCCGCGACCTCTACGACAAGGACGTGGACAACGTCTGGGTCGAGGGCGAGGCCGGCTTCA
AGGAAGCCCGCGACTTCATGCGCATGCTGATGCCGTCGCAGGCCAAGAAGGTGCAGCTCTACCGCGAGCCGACGCCGCTC
TACGTCAAGCACAAGGTCGAGGACCACCTCAGCCAGATCTATTCGCCCGTCGTGCCGCTGCGCTCCGGCGGCTACCTGGT
GATCAACCAGACCGAAGCGCTGGTCGCCATCGACGTGAACTCCGGCCGCGCGACGCGCGAACGGAACATCGAGGCCACCG
CGCTCAAGACCAACATGGAGGCGGCCGAAGAAGCCGCCCGGCAGCTGCGCCTGCGCGACCTGGCCGGCCTGATCGTCATC
GACTTCATCGACATGGATGAGGCGAAGAACAACCGGGCCGTCGAGAAGAAGCTGAAGGACTGCCTGAAGGACGACCGCGC
GCGGGTCCAGATGGGCAAGATCAGCCCCTTCGGCCTGATGGAGATCTCCCGCCAGCGCCGCCGCACCGGCGTGCTGGAGG
GCACCACCCACGTGTGCGAACACTGCGGCGGCACCGGCCGGGTCCGCTCGGTGGAATCCAGCGCGCTGGCCGCGCTGCGA
GCCCTGGAGATCGAGGCGCTGAAGGGCGGCGGCGAAGCCACCCTGACCGTGCCGCGCGCCGTGGGCCTCTACATCCTGAA
CGAGAAGCGCGGTCATCTCGCCCGCCTGCACCACGCGTTCGGCCTGTTCGTCACCGTCCTGATCGACGACGACATGGCGC
ACGCCGAGCACGGCATCGAGCGAACCGCCAGCGAGACCCGCGCCGAGCACGCCGCGGCGCTCGCGCCGACGGCCGCCGGC
GCCGTCCCCCCGGCCGAGGAGGCCTACCTCGAGGAGGTCTTCGAGGACGAGGACGAGGCCGAGGAGGAAGAGGAGTCCTT
CGAGGAGGACGAGGATGAAGGCGGCGCCGAAGCCGAGGCTCCCCGCAGCCGCGAGGCCCGGGGCGATCGGGAAGACGGCG
AAGGCCGCCGCGGGCGTCGCCGCCGTCGCCGCGGCCGCCGGGGCGACGAGCCCGCGGGCGAGCGGCCCGAGGGCGCACGT
TCGGAGGGCGGCCGTCCCGAGGGCGGGCGACGCGAGGAGGTCCAGGCCGAGGACGAGGACGGCGCCCACGGCCGTCGCCG
TCGCCGCGGCCGCCGCGGCGGTCGGCGCATGCGCGACGACTTCCGCCCGCACGAGCCCTTCTCCTGGGTGCGCCCCTGGG
TGCCTTACGGCGAAGATCCCTTCGTCTGGTACGATCCGGCCGAGGACCTGAAGCCGGTCGCCGCGCCGCCGGCTGCGGCC
AACGATGCTGCGGCCGAGCCGCAGCCCGCGGCCGCCGAGGCCGCCGCGCCCGCGCCGGCCGTCCGGGCCCCGCAGCCCGA
GGGCGACATGGAGGTCTGGGTCGAGCTCCCCGCCGAGGAGGAGAAGCCCAAGCGCGCCCGCCGCTCGCGCAGCCGCGGCC
GCGGCAAGGCCGAGGCGCAAGCCGAGGAGGCCGTGGCCGCTGAGGTCGCCGCCCCCGAGGCCGTCGTCGCGCCGGAACCG
GAGGCTCCCGCCGAGCCGGAGCCCGCTGCGGCGGAGCCGGAAGCGGCCGAGCCTGAGAAGCCCGCGCCCAAGCGCCGGAC
GCGCAGCCGCAAGGCCGCCGCGCCCGTCGAAGCCGAGGCGACGGCCGCCGAACCCGCAGCGGAGCCGGCCCGGCCTGAAC
CGGCGCCCGCGGCTCCGGCCGAGCCCGTGGCGGCTTCGGTTCCCGAGCCCGAGGCGCCGCAAGCGCCCGACCCGGCCGAG
ATCTCCGGGCCGCCGCCGGCGCCGCGCAAAGGCTGGTGGCGCCGCGGCTGA

Upstream 100 bases:

>100_bases
GCCGTTCCCGGCGCCCCGCGGGCCGGCGCGCGCGCCCGTCCCATGCACCGGCAGCGCCCGGGCCTTCGGGCCCCCGCCGC
GCGCCGCGGAGACTTACTGA

Downstream 100 bases:

>100_bases
GGCATTACATTCCGGTCGGGCTTCGAGGGCGGTCTGGAGAAGAGTTGAGCATGGTCTCCCCTGCCCGATCGGTTTCCCGC
CTCGCGATGAGCTTCGTCCT

Product: ribonuclease, Rne/Rng family protein

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 896; Mature: 895

Protein sequence:

>896_residues
MSKTMLIDAAHAEETRVVVVDGNRVEEFDFESQNRKQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQ
IPVADREALLREEAEEDDDDRHEPRARAPEDEDEVDLSGSADDEDDVVEEELARRRRRLMRRYKIQEVIRRRQIMLVQVV
KEERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITTATDRKRLKSIVQSLDVPAGMGLIVRTAGAKRTKAEIKRDY
DYLLRLWENIRETTLHSIAPALIYEEEDLVKRAIRDLYDKDVDNVWVEGEAGFKEARDFMRMLMPSQAKKVQLYREPTPL
YVKHKVEDHLSQIYSPVVPLRSGGYLVINQTEALVAIDVNSGRATRERNIEATALKTNMEAAEEAARQLRLRDLAGLIVI
DFIDMDEAKNNRAVEKKLKDCLKDDRARVQMGKISPFGLMEISRQRRRTGVLEGTTHVCEHCGGTGRVRSVESSALAALR
ALEIEALKGGGEATLTVPRAVGLYILNEKRGHLARLHHAFGLFVTVLIDDDMAHAEHGIERTASETRAEHAAALAPTAAG
AVPPAEEAYLEEVFEDEDEAEEEEESFEEDEDEGGAEAEAPRSREARGDREDGEGRRGRRRRRRGRRGDEPAGERPEGAR
SEGGRPEGGRREEVQAEDEDGAHGRRRRRGRRGGRRMRDDFRPHEPFSWVRPWVPYGEDPFVWYDPAEDLKPVAAPPAAA
NDAAAEPQPAAAEAAAPAPAVRAPQPEGDMEVWVELPAEEEKPKRARRSRSRGRGKAEAQAEEAVAAEVAAPEAVVAPEP
EAPAEPEPAAAEPEAAEPEKPAPKRRTRSRKAAAPVEAEATAAEPAAEPARPEPAPAAPAEPVAASVPEPEAPQAPDPAE
ISGPPPAPRKGWWRRG

Sequences:

>Translated_896_residues
MSKTMLIDAAHAEETRVVVVDGNRVEEFDFESQNRKQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQ
IPVADREALLREEAEEDDDDRHEPRARAPEDEDEVDLSGSADDEDDVVEEELARRRRRLMRRYKIQEVIRRRQIMLVQVV
KEERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITTATDRKRLKSIVQSLDVPAGMGLIVRTAGAKRTKAEIKRDY
DYLLRLWENIRETTLHSIAPALIYEEEDLVKRAIRDLYDKDVDNVWVEGEAGFKEARDFMRMLMPSQAKKVQLYREPTPL
YVKHKVEDHLSQIYSPVVPLRSGGYLVINQTEALVAIDVNSGRATRERNIEATALKTNMEAAEEAARQLRLRDLAGLIVI
DFIDMDEAKNNRAVEKKLKDCLKDDRARVQMGKISPFGLMEISRQRRRTGVLEGTTHVCEHCGGTGRVRSVESSALAALR
ALEIEALKGGGEATLTVPRAVGLYILNEKRGHLARLHHAFGLFVTVLIDDDMAHAEHGIERTASETRAEHAAALAPTAAG
AVPPAEEAYLEEVFEDEDEAEEEEESFEEDEDEGGAEAEAPRSREARGDREDGEGRRGRRRRRRGRRGDEPAGERPEGAR
SEGGRPEGGRREEVQAEDEDGAHGRRRRRGRRGGRRMRDDFRPHEPFSWVRPWVPYGEDPFVWYDPAEDLKPVAAPPAAA
NDAAAEPQPAAAEAAAPAPAVRAPQPEGDMEVWVELPAEEEKPKRARRSRSRGRGKAEAQAEEAVAAEVAAPEAVVAPEP
EAPAEPEPAAAEPEAAEPEKPAPKRRTRSRKAAAPVEAEATAAEPAAEPARPEPAPAAPAEPVAASVPEPEAPQAPDPAE
ISGPPPAPRKGWWRRG
>Mature_895_residues
SKTMLIDAAHAEETRVVVVDGNRVEEFDFESQNRKQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYQI
PVADREALLREEAEEDDDDRHEPRARAPEDEDEVDLSGSADDEDDVVEEELARRRRRLMRRYKIQEVIRRRQIMLVQVVK
EERGNKGAALTTYLSLAGRYGVLMPNTARGGGISRKITTATDRKRLKSIVQSLDVPAGMGLIVRTAGAKRTKAEIKRDYD
YLLRLWENIRETTLHSIAPALIYEEEDLVKRAIRDLYDKDVDNVWVEGEAGFKEARDFMRMLMPSQAKKVQLYREPTPLY
VKHKVEDHLSQIYSPVVPLRSGGYLVINQTEALVAIDVNSGRATRERNIEATALKTNMEAAEEAARQLRLRDLAGLIVID
FIDMDEAKNNRAVEKKLKDCLKDDRARVQMGKISPFGLMEISRQRRRTGVLEGTTHVCEHCGGTGRVRSVESSALAALRA
LEIEALKGGGEATLTVPRAVGLYILNEKRGHLARLHHAFGLFVTVLIDDDMAHAEHGIERTASETRAEHAAALAPTAAGA
VPPAEEAYLEEVFEDEDEAEEEEESFEEDEDEGGAEAEAPRSREARGDREDGEGRRGRRRRRRGRRGDEPAGERPEGARS
EGGRPEGGRREEVQAEDEDGAHGRRRRRGRRGGRRMRDDFRPHEPFSWVRPWVPYGEDPFVWYDPAEDLKPVAAPPAAAN
DAAAEPQPAAAEAAAPAPAVRAPQPEGDMEVWVELPAEEEKPKRARRSRSRGRGKAEAQAEEAVAAEVAAPEAVVAPEPE
APAEPEPAAAEPEAAEPEKPAPKRRTRSRKAAAPVEAEATAAEPAAEPARPEPAPAAPAEPVAASVPEPEAPQAPDPAEI
SGPPPAPRKGWWRRG

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=398, Percent_Identity=45.2261306532663, Blast_Score=337, Evalue=2e-93,
Organism=Escherichia coli, GI87082235, Length=338, Percent_Identity=35.5029585798817, Blast_Score=189, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 99426; Mature: 99295

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTMLIDAAHAEETRVVVVDGNRVEEFDFESQNRKQLRGNIYLAKVTRVEPSLQAAFVE
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFNEIHPDYYQIPVADREALLREEAEEDDDDRHEPRARAPEDEDEVDLSGS
HCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCC
ADDEDDVVEEELARRRRRLMRRYKIQEVIRRRQIMLVQVVKEERGNKGAALTTYLSLAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
YGVLMPNTARGGGISRKITTATDRKRLKSIVQSLDVPAGMGLIVRTAGAKRTKAEIKRDY
CCEECCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHH
DYLLRLWENIRETTLHSIAPALIYEEEDLVKRAIRDLYDKDVDNVWVEGEAGFKEARDFM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
RMLMPSQAKKVQLYREPTPLYVKHKVEDHLSQIYSPVVPLRSGGYLVINQTEALVAIDVN
HHHCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECC
SGRATRERNIEATALKTNMEAAEEAARQLRLRDLAGLIVIDFIDMDEAKNNRAVEKKLKD
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHH
CLKDDRARVQMGKISPFGLMEISRQRRRTGVLEGTTHVCEHCGGTGRVRSVESSALAALR
HHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHH
ALEIEALKGGGEATLTVPRAVGLYILNEKRGHLARLHHAFGLFVTVLIDDDMAHAEHGIE
HHHHHHHCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHEEEEEECCCHHHHHHHHH
RTASETRAEHAAALAPTAAGAVPPAEEAYLEEVFEDEDEAEEEEESFEEDEDEGGAEAEA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
PRSREARGDREDGEGRRGRRRRRRGRRGDEPAGERPEGARSEGGRPEGGRREEVQAEDED
CCCHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAHGRRRRRGRRGGRRMRDDFRPHEPFSWVRPWVPYGEDPFVWYDPAEDLKPVAAPPAAA
CCHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCCCCCCCCCEEEECCHHHCCCCCCCCCCC
NDAAAEPQPAAAEAAAPAPAVRAPQPEGDMEVWVELPAEEEKPKRARRSRSRGRGKAEAQ
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHH
AEEAVAAEVAAPEAVVAPEPEAPAEPEPAAAEPEAAEPEKPAPKRRTRSRKAAAPVEAEA
HHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
TAAEPAAEPARPEPAPAAPAEPVAASVPEPEAPQAPDPAEISGPPPAPRKGWWRRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTMLIDAAHAEETRVVVVDGNRVEEFDFESQNRKQLRGNIYLAKVTRVEPSLQAAFVE
CCEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHHHCCCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFNEIHPDYYQIPVADREALLREEAEEDDDDRHEPRARAPEDEDEVDLSGS
HCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCC
ADDEDDVVEEELARRRRRLMRRYKIQEVIRRRQIMLVQVVKEERGNKGAALTTYLSLAGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
YGVLMPNTARGGGISRKITTATDRKRLKSIVQSLDVPAGMGLIVRTAGAKRTKAEIKRDY
CCEECCCCCCCCCCCCEEECHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHH
DYLLRLWENIRETTLHSIAPALIYEEEDLVKRAIRDLYDKDVDNVWVEGEAGFKEARDFM
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
RMLMPSQAKKVQLYREPTPLYVKHKVEDHLSQIYSPVVPLRSGGYLVINQTEALVAIDVN
HHHCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCEEEEECCCEEEEEECC
SGRATRERNIEATALKTNMEAAEEAARQLRLRDLAGLIVIDFIDMDEAKNNRAVEKKLKD
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHH
CLKDDRARVQMGKISPFGLMEISRQRRRTGVLEGTTHVCEHCGGTGRVRSVESSALAALR
HHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHH
ALEIEALKGGGEATLTVPRAVGLYILNEKRGHLARLHHAFGLFVTVLIDDDMAHAEHGIE
HHHHHHHCCCCCEEEEECCEEEEEEECCCCCHHHHHHHHHHHEEEEEECCCHHHHHHHHH
RTASETRAEHAAALAPTAAGAVPPAEEAYLEEVFEDEDEAEEEEESFEEDEDEGGAEAEA
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCC
PRSREARGDREDGEGRRGRRRRRRGRRGDEPAGERPEGARSEGGRPEGGRREEVQAEDED
CCCHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAHGRRRRRGRRGGRRMRDDFRPHEPFSWVRPWVPYGEDPFVWYDPAEDLKPVAAPPAAA
CCHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCCCCCCCCCEEEECCHHHCCCCCCCCCCC
NDAAAEPQPAAAEAAAPAPAVRAPQPEGDMEVWVELPAEEEKPKRARRSRSRGRGKAEAQ
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHH
AEEAVAAEVAAPEAVVAPEPEAPAEPEPAAAEPEAAEPEKPAPKRRTRSRKAAAPVEAEA
HHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
TAAEPAAEPARPEPAPAAPAEPVAASVPEPEAPQAPDPAEISGPPPAPRKGWWRRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]