Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 197105253

GI number: 197105253

Start: 2003855

End: 2004595

Strand: Reverse

Name: birA [H]

Synonym: PHZ_c1790

Alternate gene names: 197105253

Gene position: 2004595-2003855 (Counterclockwise)

Preceding gene: 197105254

Following gene: 197105252

Centisome position: 50.16

GC content: 77.19

Gene sequence:

>741_bases
TTGGCCGAGCACCCGCCGATCGAGGCCTACGAGGCGCTCGACTCCACCAACGCCGAGGCCCGCCGCCGGGCCGAGGCGGG
CGAGGCGGGGCCCGTCTGGATCACCGCGGCGGTCCAGACCGCCGGCCGCGGCCGCCGCGGGCGGGCCTGGTCCACCCGCA
AGGGGAACCTCGCCGCGACCCTGCTGACCGTCACGGACGTCCCGGCCGCCGAGGCCGCCCAGCTCTCGTTCGTGGCGGCG
CTCGCCGCCTGCGACCTGGCCGACGCCTGCCTCGGTGAGGGGACCGCGCGGCTGAAATGGCCCAACGACGTGCTGATCCA
CGGCCGCAAGGCGGTCGGAATCTTGGTCGAGTCCGGCGCTCGGCCGGACGGGCGGCTGTGGCTCGCGGTGGGGATCGGAG
TGAACCTCGCCCATGCGCCGCAGGACGTGGAGCGTCCCGCCGCCGCCTTCGCCGAGTACATGGCCGGCCCGCCGCCCGAG
CCGCGGGCCGCGCTGGAGGTCCTGGCCGCCGCGTTCGAACGCTGGCGGCGGACCTGGGCGGCGGGCGGCTTCGCGCCCAT
CGCGCAGGCGTGGACGGCGCGGGCCATGGGTCTGGGCGAGCGCTGCGAGGCGCGGCTGCCGGGCCGGACGCTCGCGGGCG
TGGCCGAGGCGCTGGATTCCGACGGCGCGCTGCGGCTCAGGCTGGACGACGGGAGCCTGGAGCGCATAACGGCGGGCGAC
GTGTTCTTCGGGGGCGTGTGA

Upstream 100 bases:

>100_bases
GCGCTGGGCCTCTTCTCCTTCCCCGTGGTGCTCGTGGCCCTGGTCTGGATCTACCCGGCCGCCGGCCAGGCCGCGCGCGC
CTTCGGCCTGTCCTAAGGGC

Downstream 100 bases:

>100_bases
GACGAATGTTGCTGGCGATCGAACAGGGGAACACCAACACCCTCTTCGCGGTGCATGACGGCGAACGCTGGATCGCCCAG
TGGCGCGCGGCGACCGATTC

Product: biotin protein ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAEHPPIEAYEALDSTNAEARRRAEAGEAGPVWITAAVQTAGRGRRGRAWSTRKGNLAATLLTVTDVPAAEAAQLSFVAA
LAACDLADACLGEGTARLKWPNDVLIHGRKAVGILVESGARPDGRLWLAVGIGVNLAHAPQDVERPAAAFAEYMAGPPPE
PRAALEVLAAAFERWRRTWAAGGFAPIAQAWTARAMGLGERCEARLPGRTLAGVAEALDSDGALRLRLDDGSLERITAGD
VFFGGV

Sequences:

>Translated_246_residues
MAEHPPIEAYEALDSTNAEARRRAEAGEAGPVWITAAVQTAGRGRRGRAWSTRKGNLAATLLTVTDVPAAEAAQLSFVAA
LAACDLADACLGEGTARLKWPNDVLIHGRKAVGILVESGARPDGRLWLAVGIGVNLAHAPQDVERPAAAFAEYMAGPPPE
PRAALEVLAAAFERWRRTWAAGGFAPIAQAWTARAMGLGERCEARLPGRTLAGVAEALDSDGALRLRLDDGSLERITAGD
VFFGGV
>Mature_245_residues
AEHPPIEAYEALDSTNAEARRRAEAGEAGPVWITAAVQTAGRGRRGRAWSTRKGNLAATLLTVTDVPAAEAAQLSFVAAL
AACDLADACLGEGTARLKWPNDVLIHGRKAVGILVESGARPDGRLWLAVGIGVNLAHAPQDVERPAAAFAEYMAGPPPEP
RAALEVLAAAFERWRRTWAAGGFAPIAQAWTARAMGLGERCEARLPGRTLAGVAEALDSDGALRLRLDDGSLERITAGDV
FFGGV

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=224, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 25793; Mature: 25662

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEHPPIEAYEALDSTNAEARRRAEAGEAGPVWITAAVQTAGRGRRGRAWSTRKGNLAAT
CCCCCCCHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCEEEE
LLTVTDVPAAEAAQLSFVAALAACDLADACLGEGTARLKWPNDVLIHGRKAVGILVESGA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCEEEEEEECCC
RPDGRLWLAVGIGVNLAHAPQDVERPAAAFAEYMAGPPPEPRAALEVLAAAFERWRRTWA
CCCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AGGFAPIAQAWTARAMGLGERCEARLPGRTLAGVAEALDSDGALRLRLDDGSLERITAGD
CCCCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECC
VFFGGV
EEECCC
>Mature Secondary Structure 
AEHPPIEAYEALDSTNAEARRRAEAGEAGPVWITAAVQTAGRGRRGRAWSTRKGNLAAT
CCCCCCHHHHHHCCCCHHHHHHHCCCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCEEEE
LLTVTDVPAAEAAQLSFVAALAACDLADACLGEGTARLKWPNDVLIHGRKAVGILVESGA
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECCCEEEEEEECCC
RPDGRLWLAVGIGVNLAHAPQDVERPAAAFAEYMAGPPPEPRAALEVLAAAFERWRRTWA
CCCCCEEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
AGGFAPIAQAWTARAMGLGERCEARLPGRTLAGVAEALDSDGALRLRLDDGSLERITAGD
CCCCHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECC
VFFGGV
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]