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The map label for this gene is ispDF [H]

Identifier: 197105196

GI number: 197105196

Start: 1941600

End: 1942745

Strand: Direct

Name: ispDF [H]

Synonym: PHZ_c1733

Alternate gene names: 197105196

Gene position: 1941600-1942745 (Clockwise)

Preceding gene: 197105187

Following gene: 197105198

Centisome position: 48.59

GC content: 73.21

Gene sequence:

>1146_bases
ATGAGTTTCTCGGCTGTTGTCGTGGCGGCGGGGGCCGGTCTGAGGGCCGGTCCGGGCGAACCCAAGGCCTGGCGCACCCT
GGGCGGACGGGCGCTCGTGCGCTGGTCGGTGGAGGGCCTGCTGTCGGCCGGCGCGCGCGAGGTCGTGGTCGTGGTCGCCC
ACGATCGCCTGACGCAGGTGGACGAGGCCCTGGCGGGGCTGGTCGGCTGGCGCGCCGTCACCGGCGGCAAGACCCGGGCC
GAGTCGGTGCAGGCCGGGTTGGCGGCGATCACCGCCGGCCGGACCCAACCGGTGCTGATCCACGACGCCGCGCGACCCTT
CGTGACGAAGGAGCATGTCGACCGCCTGCTGAAGGCGCTGGAGGTCTCCGACGGCGCGATCCCCGCCCTGCCCGTCCCCG
ACACGCTGAAGCGCGGCGACGGTCTGGTGGACGAGACGGTCTCCCGCGACGGGCTCTGGCGTGCCCAGACGCCCCAGGCG
TTCCGGACCGGCAAGCTGAAGGCGGCCTACCGCCTCTGGCCCACGGGCGAAGAGCCGACCGACGACGCCTCGGTCATGGA
GCGGGCCGGCGGCTCGGTGGCCATGGTCCCCGGCGATCCGCTGCTGATGAAGCTGACCTATCCGCAGGACTTCCTGATGG
CCGAACAACTGGCCGGCGGCCGCCGCATCGTCCGCATGGGCCAAGGGATCGACGCCCACCGCTTCGGCCCCGGCGAAGTC
GTCTGGCTGTGCGGCGTCCGCATCCAGCACGACCTGGGGCTCGTGGGCCATTCCGACGCGGATTGCGGCCTCCACGCGCT
CACCGATGCGGTGCTGGGCGCGATCGCCCAGGGGGACATCGGCGAGCACTTCCCGCCCTCGGACCCGCGGTGGAAGGGCG
CCTCCTCCGACCGGTTCCTGAAGCATGCGGTGGACCTGGTGGGCGCGGCCGGCGGGCGGATCCTCAACGCCGACGTGACG
CTCGTCTGCGAACGCCCGAAGATCCGGCCGCACCGCGACGCCATGCGGGCCCGGATCGCCGAACTGCTGGGCCTGCCGGT
CGAGCGGGTCAGCGTGAAGGCCACCACGACCGAGGGCATGGGCTTCACCGGCCGCGAGGAAGGCCTGATGGCCCAGGCCG
TCGTCGCGGTCGAATGCCCGCCCTGA

Upstream 100 bases:

>100_bases
TGGTGCTCATTTTCTAATCATCCCCGACGCTGTGTTTTTAGGCAATTTGTGCGGCGCCGTAAAGCTGGAAGAAAGCGCCG
CAGTCCCTTAGACCGACGCC

Downstream 100 bases:

>100_bases
CGGCTACGGCAGCCAGGTCCAGCGGGTCTTGAGGTTGGCGTAGAGCGCCCCGGCCAGGTTCGTGTAGTCGTCGCGCCAGG
GCCGGGCCAGGAACGGATTG

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSFSAVVVAAGAGLRAGPGEPKAWRTLGGRALVRWSVEGLLSAGAREVVVVVAHDRLTQVDEALAGLVGWRAVTGGKTRA
ESVQAGLAAITAGRTQPVLIHDAARPFVTKEHVDRLLKALEVSDGAIPALPVPDTLKRGDGLVDETVSRDGLWRAQTPQA
FRTGKLKAAYRLWPTGEEPTDDASVMERAGGSVAMVPGDPLLMKLTYPQDFLMAEQLAGGRRIVRMGQGIDAHRFGPGEV
VWLCGVRIQHDLGLVGHSDADCGLHALTDAVLGAIAQGDIGEHFPPSDPRWKGASSDRFLKHAVDLVGAAGGRILNADVT
LVCERPKIRPHRDAMRARIAELLGLPVERVSVKATTTEGMGFTGREEGLMAQAVVAVECPP

Sequences:

>Translated_381_residues
MSFSAVVVAAGAGLRAGPGEPKAWRTLGGRALVRWSVEGLLSAGAREVVVVVAHDRLTQVDEALAGLVGWRAVTGGKTRA
ESVQAGLAAITAGRTQPVLIHDAARPFVTKEHVDRLLKALEVSDGAIPALPVPDTLKRGDGLVDETVSRDGLWRAQTPQA
FRTGKLKAAYRLWPTGEEPTDDASVMERAGGSVAMVPGDPLLMKLTYPQDFLMAEQLAGGRRIVRMGQGIDAHRFGPGEV
VWLCGVRIQHDLGLVGHSDADCGLHALTDAVLGAIAQGDIGEHFPPSDPRWKGASSDRFLKHAVDLVGAAGGRILNADVT
LVCERPKIRPHRDAMRARIAELLGLPVERVSVKATTTEGMGFTGREEGLMAQAVVAVECPP
>Mature_380_residues
SFSAVVVAAGAGLRAGPGEPKAWRTLGGRALVRWSVEGLLSAGAREVVVVVAHDRLTQVDEALAGLVGWRAVTGGKTRAE
SVQAGLAAITAGRTQPVLIHDAARPFVTKEHVDRLLKALEVSDGAIPALPVPDTLKRGDGLVDETVSRDGLWRAQTPQAF
RTGKLKAAYRLWPTGEEPTDDASVMERAGGSVAMVPGDPLLMKLTYPQDFLMAEQLAGGRRIVRMGQGIDAHRFGPGEVV
WLCGVRIQHDLGLVGHSDADCGLHALTDAVLGAIAQGDIGEHFPPSDPRWKGASSDRFLKHAVDLVGAAGGRILNADVTL
VCERPKIRPHRDAMRARIAELLGLPVERVSVKATTTEGMGFTGREEGLMAQAVVAVECPP

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=151, Percent_Identity=50.3311258278146, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789104, Length=223, Percent_Identity=38.5650224215247, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 40333; Mature: 40202

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSAVVVAAGAGLRAGPGEPKAWRTLGGRALVRWSVEGLLSAGAREVVVVVAHDRLTQV
CCCCEEEEEECCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHCCCCCEEEEEEECCHHHHH
DEALAGLVGWRAVTGGKTRAESVQAGLAAITAGRTQPVLIHDAARPFVTKEHVDRLLKAL
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
EVSDGAIPALPVPDTLKRGDGLVDETVSRDGLWRAQTPQAFRTGKLKAAYRLWPTGEEPT
HCCCCCCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCC
DDASVMERAGGSVAMVPGDPLLMKLTYPQDFLMAEQLAGGRRIVRMGQGIDAHRFGPGEV
CHHHHHHHCCCCEEEECCCCEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCE
VWLCGVRIQHDLGLVGHSDADCGLHALTDAVLGAIAQGDIGEHFPPSDPRWKGASSDRFL
EEEECCEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
KHAVDLVGAAGGRILNADVTLVCERPKIRPHRDAMRARIAELLGLPVERVSVKATTTEGM
HHHHHHHHCCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCC
GFTGREEGLMAQAVVAVECPP
CCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
SFSAVVVAAGAGLRAGPGEPKAWRTLGGRALVRWSVEGLLSAGAREVVVVVAHDRLTQV
CCCEEEEEECCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHCCCCCEEEEEEECCHHHHH
DEALAGLVGWRAVTGGKTRAESVQAGLAAITAGRTQPVLIHDAARPFVTKEHVDRLLKAL
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
EVSDGAIPALPVPDTLKRGDGLVDETVSRDGLWRAQTPQAFRTGKLKAAYRLWPTGEEPT
HCCCCCCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCCC
DDASVMERAGGSVAMVPGDPLLMKLTYPQDFLMAEQLAGGRRIVRMGQGIDAHRFGPGEV
CHHHHHHHCCCCEEEECCCCEEEEEECCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCE
VWLCGVRIQHDLGLVGHSDADCGLHALTDAVLGAIAQGDIGEHFPPSDPRWKGASSDRFL
EEEECCEEECCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH
KHAVDLVGAAGGRILNADVTLVCERPKIRPHRDAMRARIAELLGLPVERVSVKATTTEGM
HHHHHHHHCCCCEEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEEECCCC
GFTGREEGLMAQAVVAVECPP
CCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA