Click here to switch to the map view.

The map label for this gene is nifR3 [H]

Identifier: 197105195

GI number: 197105195

Start: 1940505

End: 1941509

Strand: Reverse

Name: nifR3 [H]

Synonym: PHZ_c1732

Alternate gene names: 197105195

Gene position: 1941509-1940505 (Counterclockwise)

Preceding gene: 197105197

Following gene: 197105194

Centisome position: 48.58

GC content: 72.64

Gene sequence:

>1005_bases
ATGAGCACCATGCTCAAGATCGGCGGAGTCGAGGTCGGCGGGCGGGCGTGGATCGCGCCCATGACCGGCGTGTCCGACCT
GCCGTTCCGAAAGGCCGCCAGCGCCCAGGGCGCGGCCTATGTGGCGACCGAGATGGTCGCGTGCGCCGAGTTCTCGAAGG
GCCGGCCCGACGTGGTCCGACGCGCGGCGGTGGGCGAGGGCCTGCCTTTGATGGTCGTCCAGCTCGTCGGCCGCGATCCC
GAACACATCCGGCAAGGCGCGCGCCTCGCCGCCCAGGCCGGCGCGCAGATCGTCGACCTCAACTTCGGCTGTCCGGCCAA
GGAGGTCACCGGCGTCCTCTCGGGCTCGGCCCTGATGCGGGAGCCCGAGCTGGCCGCCCGCCTGATGGCCGCCGCGGTCG
AGGCGGTGGACGTGCCGGTGACCGTCAAGATGCGCCTGGGCTGGGACGACGCCTCGCGGAACGCCCCCGAGATCGCCGCG
GCCGCCGAGGCGATCGGCGTCCAGGCGGTGACGGTCCACGGCCGCACGCGCTGCCAGTTCTACAAGGGCGCCGCCGACTG
GAGCGCCGTCCGGGCGGTGAAGGCGGCGGTGTCCATCCCGGTGATCGTCAACGGCGACATCGTGGATGGGACCACGGCCA
AGGCGGCCCTGGCGGCCTCGGGCGCGGACGCCGTGATGGTCGGCCGCGGAGTCTACGGCAAGCCTTGGGTCGCGGCGCAG
ATCGAGGCCGAGCTGGCGGGGCGCCGCTTCGAGGAGCCGGATGCTCCGGCGCGGCTGGAGATCGCCCTCAGCCACTTCCG
CGAGAGCCTGAAGTTCTACGGCGACCGCCTGGGCCTGCGGATCTTCCGCAAGCATCTGGCGAGCTACATCGAAAACGCCC
CCTGGCCCGCCGATCCGCAGGCCAGGCGAGAAGCGCGCGCGGCCCTGTGCCGCCTGGAGGACGCCTCTGAAGTGGAGCGG
GGCCTGAAGGCCCTGTGGGGCGCTTCGGAGAGATTGGCGGCATGA

Upstream 100 bases:

>100_bases
AGAAACTCATGGCGTCGGTCTAAGGGACTGCGGCGCTTTCTTCCAGCTTTACGGCGCCGCACAAATTGCCTAAAAACACA
GCGTCGGGGATGATTAGAAA

Downstream 100 bases:

>100_bases
ACGGAAACACGAGGACGCACCCCAAGGGGATAGATCCCGACGCCCTGAAGGCGGCGGCGTTCGACCTCAGTCCCGACCCT
TCGCTGGTGGTCAGCGCCGA

Product: tRNA-dihydrouridine synthase, nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSTMLKIGGVEVGGRAWIAPMTGVSDLPFRKAASAQGAAYVATEMVACAEFSKGRPDVVRRAAVGEGLPLMVVQLVGRDP
EHIRQGARLAAQAGAQIVDLNFGCPAKEVTGVLSGSALMREPELAARLMAAAVEAVDVPVTVKMRLGWDDASRNAPEIAA
AAEAIGVQAVTVHGRTRCQFYKGAADWSAVRAVKAAVSIPVIVNGDIVDGTTAKAALAASGADAVMVGRGVYGKPWVAAQ
IEAELAGRRFEEPDAPARLEIALSHFRESLKFYGDRLGLRIFRKHLASYIENAPWPADPQARREARAALCRLEDASEVER
GLKALWGASERLAA

Sequences:

>Translated_334_residues
MSTMLKIGGVEVGGRAWIAPMTGVSDLPFRKAASAQGAAYVATEMVACAEFSKGRPDVVRRAAVGEGLPLMVVQLVGRDP
EHIRQGARLAAQAGAQIVDLNFGCPAKEVTGVLSGSALMREPELAARLMAAAVEAVDVPVTVKMRLGWDDASRNAPEIAA
AAEAIGVQAVTVHGRTRCQFYKGAADWSAVRAVKAAVSIPVIVNGDIVDGTTAKAALAASGADAVMVGRGVYGKPWVAAQ
IEAELAGRRFEEPDAPARLEIALSHFRESLKFYGDRLGLRIFRKHLASYIENAPWPADPQARREARAALCRLEDASEVER
GLKALWGASERLAA
>Mature_333_residues
STMLKIGGVEVGGRAWIAPMTGVSDLPFRKAASAQGAAYVATEMVACAEFSKGRPDVVRRAAVGEGLPLMVVQLVGRDPE
HIRQGARLAAQAGAQIVDLNFGCPAKEVTGVLSGSALMREPELAARLMAAAVEAVDVPVTVKMRLGWDDASRNAPEIAAA
AEAIGVQAVTVHGRTRCQFYKGAADWSAVRAVKAAVSIPVIVNGDIVDGTTAKAALAASGADAVMVGRGVYGKPWVAAQI
EAELAGRRFEEPDAPARLEIALSHFRESLKFYGDRLGLRIFRKHLASYIENAPWPADPQARREARAALCRLEDASEVERG
LKALWGASERLAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=287, Percent_Identity=28.9198606271777, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI239788462, Length=287, Percent_Identity=28.9198606271777, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI40807366, Length=249, Percent_Identity=33.7349397590361, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI31742496, Length=265, Percent_Identity=27.9245283018868, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789660, Length=328, Percent_Identity=40.8536585365854, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1788462, Length=288, Percent_Identity=34.0277777777778, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI145693211, Length=261, Percent_Identity=29.8850574712644, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=31.5315315315315, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=294, Percent_Identity=24.8299319727891, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=227, Percent_Identity=31.7180616740088, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323433, Length=319, Percent_Identity=25.3918495297806, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=225, Percent_Identity=29.3333333333333, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24585320, Length=298, Percent_Identity=31.8791946308725, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=32.3008849557522, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=32.3008849557522, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19921524, Length=313, Percent_Identity=25.2396166134185, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45549423, Length=240, Percent_Identity=28.3333333333333, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35317; Mature: 35186

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMLKIGGVEVGGRAWIAPMTGVSDLPFRKAASAQGAAYVATEMVACAEFSKGRPDVVR
CCCEEEECCEEECCEEEECCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
RAAVGEGLPLMVVQLVGRDPEHIRQGARLAAQAGAQIVDLNFGCPAKEVTGVLSGSALMR
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCHHHHC
EPELAARLMAAAVEAVDVPVTVKMRLGWDDASRNAPEIAAAAEAIGVQAVTVHGRTRCQF
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCEEEEEECCCEEEEE
YKGAADWSAVRAVKAAVSIPVIVNGDIVDGTTAKAALAASGADAVMVGRGVYGKPWVAAQ
ECCCCCHHHHHHHHHHHCCCEEEECCEECCCCHHHHHHCCCCCEEEECCCCCCCCCEEEE
IEAELAGRRFEEPDAPARLEIALSHFRESLKFYGDRLGLRIFRKHLASYIENAPWPADPQ
EHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ARREARAALCRLEDASEVERGLKALWGASERLAA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
STMLKIGGVEVGGRAWIAPMTGVSDLPFRKAASAQGAAYVATEMVACAEFSKGRPDVVR
CCEEEECCEEECCEEEECCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
RAAVGEGLPLMVVQLVGRDPEHIRQGARLAAQAGAQIVDLNFGCPAKEVTGVLSGSALMR
HHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCHHHHC
EPELAARLMAAAVEAVDVPVTVKMRLGWDDASRNAPEIAAAAEAIGVQAVTVHGRTRCQF
CHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHCEEEEEECCCEEEEE
YKGAADWSAVRAVKAAVSIPVIVNGDIVDGTTAKAALAASGADAVMVGRGVYGKPWVAAQ
ECCCCCHHHHHHHHHHHCCCEEEECCEECCCCHHHHHHCCCCCEEEECCCCCCCCCEEEE
IEAELAGRRFEEPDAPARLEIALSHFRESLKFYGDRLGLRIFRKHLASYIENAPWPADPQ
EHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
ARREARAALCRLEDASEVERGLKALWGASERLAA
HHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]