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The map label for this gene is ntrX [H]

Identifier: 197105191

GI number: 197105191

Start: 1934275

End: 1935666

Strand: Reverse

Name: ntrX [H]

Synonym: PHZ_c1728

Alternate gene names: 197105191

Gene position: 1935666-1934275 (Counterclockwise)

Preceding gene: 197105192

Following gene: 197105190

Centisome position: 48.44

GC content: 70.69

Gene sequence:

>1392_bases
ATGGCTGCAGACGTTCTGGTGGTCGACGACGAGGTGGACATCCGCGAGCTGGTCGCGGGGATCCTCTCGGACGAGGGTTA
TGCGGTCCGGACGGCGAACGACTCCGAATCGGCGCTGGCCGCGGTCCGCGCGCGCAAGCCCGCGCTCCTGATCCTCGACA
TCTGGATGTCGGGCGGCGGGATGGACGGCCTGGAGCTGCTCGATCTCGTCAAGGAGCTCGATCCCGACCTTCCGGTGATC
ATGATCTCGGGCCACGGCAACATCGAGACGGCGGTCAGCGCCATCAAGCGCGGCGCCTACGAGTTCCTGGAGAAGCCGTT
CAAGTCCGACCGGCTGCTGCTGGTCGTGGAACGGGCGCTGGAGGCGGCCAACCTGCGGCGCGAGAACCGCCGGCTGCGCG
CCCAGGCCATCACGCCTGAGGGACTGCTCGGCAAGTCGGTCGCCGCCCAGCAGCTCCGCCAGATGATCGCCAAGCTCGCG
GGCGCCAACAGCCGCGTCCTGATCTCGGGCCCGCCGGGCGCGGGCAAGGAGACGGTCGCGCGCCTGATCCATGACGCCAG
CCATCGGGCCCGCAACGAGTTCGTGCCGATCAGCGCCGCCGGCATGACGCCCGAGCGCATGGACATCGAGCTGTTCGGCC
AGGAGGGCGAGGGCGGACGTCCGGCCAAGATCGGGGTGTTCGAACGGGCCCACGCGGGCACGCTCTACCTCGACGAGGTC
GCCGACATGCCGCGGGAGACCCAGGGCCGCATCCTGCGGGTGCTGGTGGAGCAGCGGTTCCGGCGCGTGGACGGCGACTC
GGACGTCCAGGTGGACGTGCGGGTGATCTCGTCCACTTCCAAGGACCTGCGCGAGGAGATCGCCGCCGGCCGCTTCCGCG
AGGACCTGTTCCACCGCCTGAACGTGGTGCCGGTGAACGTGCCGGGCCTGGCCGAGCGGCGCGAGGACATCCCCGAGCTG
ATCGACGACTTCATCACCCGCATCTGCGACGCCACCGGCCTGCCGCGGCGGCGGCTGTCCGACGACGCGCTCGCCATGCT
CCAGGTGCGCGCCTGGCCCGGCAACCTGCGCCAGCTGCGCAACAACGTGGAGCGGATGCTGATCCTGGCGTCGGGCGCGC
CGTCCGAGCCGATCACCGCCGAGATGCTGCCGCCCGAGGCTTCCGTCAGCGACCAGGCCGGCTCGTTCGGCGCCGAGCGG
ATCATCGCCCTGCCGCTGCGCGAGGCCCGCGAGGTGTTCGAGCGCGAGTACCTGAACGCCCAGATGCTCCGCTTCTCGGG
CAACATCAGCCGCACCGCCGCCTTCATCGGCATGGAGCGTTCGGCCCTGCACCGGAAGCTGAAGTCGCTGGGGCTCTCCG
GTGCCCGCCCCCTCGAGACCGAGGAGGCCTGA

Upstream 100 bases:

>100_bases
AGCGGGCAGGGGGCCCTGGCCACCCTTCGCCTGCCGGCGGCGAGCGCCGCGCGCGTCGAGGCTACGGCCGTCGCCGCGAC
GACGTGAGAAGGCGGAAGGC

Downstream 100 bases:

>100_bases
GCTTGGGCCGCATCGCCTACGTCAACGGCCGCTTCGTTCCCCACGGGGAGGCGTTCGTCCACATCGAGGACCGCGGCTAT
CAGTTCGCCGACGCGGTCTA

Product: nitrogen regulation protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAADVLVVDDEVDIRELVAGILSDEGYAVRTANDSESALAAVRARKPALLILDIWMSGGGMDGLELLDLVKELDPDLPVI
MISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLVVERALEAANLRRENRRLRAQAITPEGLLGKSVAAQQLRQMIAKLA
GANSRVLISGPPGAGKETVARLIHDASHRARNEFVPISAAGMTPERMDIELFGQEGEGGRPAKIGVFERAHAGTLYLDEV
ADMPRETQGRILRVLVEQRFRRVDGDSDVQVDVRVISSTSKDLREEIAAGRFREDLFHRLNVVPVNVPGLAERREDIPEL
IDDFITRICDATGLPRRRLSDDALAMLQVRAWPGNLRQLRNNVERMLILASGAPSEPITAEMLPPEASVSDQAGSFGAER
IIALPLREAREVFEREYLNAQMLRFSGNISRTAAFIGMERSALHRKLKSLGLSGARPLETEEA

Sequences:

>Translated_463_residues
MAADVLVVDDEVDIRELVAGILSDEGYAVRTANDSESALAAVRARKPALLILDIWMSGGGMDGLELLDLVKELDPDLPVI
MISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLVVERALEAANLRRENRRLRAQAITPEGLLGKSVAAQQLRQMIAKLA
GANSRVLISGPPGAGKETVARLIHDASHRARNEFVPISAAGMTPERMDIELFGQEGEGGRPAKIGVFERAHAGTLYLDEV
ADMPRETQGRILRVLVEQRFRRVDGDSDVQVDVRVISSTSKDLREEIAAGRFREDLFHRLNVVPVNVPGLAERREDIPEL
IDDFITRICDATGLPRRRLSDDALAMLQVRAWPGNLRQLRNNVERMLILASGAPSEPITAEMLPPEASVSDQAGSFGAER
IIALPLREAREVFEREYLNAQMLRFSGNISRTAAFIGMERSALHRKLKSLGLSGARPLETEEA
>Mature_462_residues
AADVLVVDDEVDIRELVAGILSDEGYAVRTANDSESALAAVRARKPALLILDIWMSGGGMDGLELLDLVKELDPDLPVIM
ISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLVVERALEAANLRRENRRLRAQAITPEGLLGKSVAAQQLRQMIAKLAG
ANSRVLISGPPGAGKETVARLIHDASHRARNEFVPISAAGMTPERMDIELFGQEGEGGRPAKIGVFERAHAGTLYLDEVA
DMPRETQGRILRVLVEQRFRRVDGDSDVQVDVRVISSTSKDLREEIAAGRFREDLFHRLNVVPVNVPGLAERREDIPELI
DDFITRICDATGLPRRRLSDDALAMLQVRAWPGNLRQLRNNVERMLILASGAPSEPITAEMLPPEASVSDQAGSFGAERI
IALPLREAREVFEREYLNAQMLRFSGNISRTAAFIGMERSALHRKLKSLGLSGARPLETEEA

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=36.3829787234043, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=34.4226579520697, Blast_Score=268, Evalue=7e-73,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=36.4653243847875, Blast_Score=251, Evalue=7e-68,
Organism=Escherichia coli, GI1788905, Length=449, Percent_Identity=34.075723830735, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=37.1517027863777, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1787583, Length=299, Percent_Identity=37.123745819398, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789087, Length=320, Percent_Identity=35, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789233, Length=251, Percent_Identity=38.2470119521912, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1786524, Length=329, Percent_Identity=36.1702127659575, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87082117, Length=255, Percent_Identity=37.6470588235294, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=31.5625, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789828, Length=217, Percent_Identity=33.1797235023041, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=28.5223367697594, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50905; Mature: 50774

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADVLVVDDEVDIRELVAGILSDEGYAVRTANDSESALAAVRARKPALLILDIWMSGGG
CCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCC
MDGLELLDLVKELDPDLPVIMISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLVVERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
EAANLRRENRRLRAQAITPEGLLGKSVAAQQLRQMIAKLAGANSRVLISGPPGAGKETVA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RLIHDASHRARNEFVPISAAGMTPERMDIELFGQEGEGGRPAKIGVFERAHAGTLYLDEV
HHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEEHHHH
ADMPRETQGRILRVLVEQRFRRVDGDSDVQVDVRVISSTSKDLREEIAAGRFREDLFHRL
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
NVVPVNVPGLAERREDIPELIDDFITRICDATGLPRRRLSDDALAMLQVRAWPGNLRQLR
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHEEEEEEECCCCHHHHHH
NNVERMLILASGAPSEPITAEMLPPEASVSDQAGSFGAERIIALPLREAREVFEREYLNA
HHHHHEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCH
QMLRFSGNISRTAAFIGMERSALHRKLKSLGLSGARPLETEEA
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
AADVLVVDDEVDIRELVAGILSDEGYAVRTANDSESALAAVRARKPALLILDIWMSGGG
CCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEEEECCCC
MDGLELLDLVKELDPDLPVIMISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLVVERAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHH
EAANLRRENRRLRAQAITPEGLLGKSVAAQQLRQMIAKLAGANSRVLISGPPGAGKETVA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
RLIHDASHRARNEFVPISAAGMTPERMDIELFGQEGEGGRPAKIGVFERAHAGTLYLDEV
HHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCCCEEHHHH
ADMPRETQGRILRVLVEQRFRRVDGDSDVQVDVRVISSTSKDLREEIAAGRFREDLFHRL
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHC
NVVPVNVPGLAERREDIPELIDDFITRICDATGLPRRRLSDDALAMLQVRAWPGNLRQLR
CEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHEEEEEEECCCCHHHHHH
NNVERMLILASGAPSEPITAEMLPPEASVSDQAGSFGAERIIALPLREAREVFEREYLNA
HHHHHEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHCH
QMLRFSGNISRTAAFIGMERSALHRKLKSLGLSGARPLETEEA
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]