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The map label for this gene is ksgA

Identifier: 197105173

GI number: 197105173

Start: 1918582

End: 1919439

Strand: Direct

Name: ksgA

Synonym: PHZ_c1710

Alternate gene names: 197105173

Gene position: 1918582-1919439 (Clockwise)

Preceding gene: 197105172

Following gene: 197105174

Centisome position: 48.01

GC content: 74.83

Gene sequence:

>858_bases
ATGAGCCTGGAGACCCTGCCCACCCTGCGCGAGGCGCTGGAGGCGCACGGCCTGTGGGCCAAGAAGGCGTTCGGCCAGCA
CTTCCTCCTCGATCTCAACATCACCCGCAAGATCGCGCGCCTGGCGCAGGTGGGCGACGGCGACGTGGTGATCGAGGTGG
GCCCGGGCCCCGGCGGCCTGACCCGCGCTCTGCTGGAGACCGGCGCCCGGGTCATCGCCGTCGAGAAGGACGAACGCTTC
CGGCCGCTGCTGCAGGAGGTGGCCGACGCCGCCCCGCATCTCACCCTGGTCTTCGGGGATGCGTTGACGGCCGACGAGGC
GGCGCTGTCGGCCGGCCGGCCCGCGCACCTCGTCTCGAACCTGCCCTACAACGTCGGCACGCCGCTGCTCATCAAGTGGC
TGACCGGCCCCTGGACGCCGGCGTCGCTGACCCTGATGTTCCAGAAGGAGGTCGCCGACCGGATCACGGCGGCGCCGGGC
GAAGACGCCTACGGCCGGCTGGCGGTGATCGCCCAGGCCACCGCGGACGCCCGGCCGGTGATGGACGTCCCGGCCCGCGC
CTTCACCCCGCCGCCGAAGGTGGAATCGGCCGTGGTCCGCCTCGAGCCGCGGGCCGCCCGCCCCTCGCCCGAGCGCCTGG
ACGCCCTGCAGAAGGTCACCGCCGCCGCCTTCGGTCAGCGCCGCAAGATGCTCCGCTCCAGCCTCAAGGCCCTGGGGGGC
GAGCCGCTCATCACGGCCGCGGGCCTGGACCCGGCCGCCCGCGCCGAGGTCGTGCCGGTGGCGGGTTTCCTGGCGCTCGC
CGACGCCTGGCTGGCGCGGCGCGAAGCCCCGGCGACGGCCGCTCCTCGTGGACGCTGA

Upstream 100 bases:

>100_bases
ACGGCACGGCGTTCGACATCGCCGGCCGGGGGATCGCGCGGCCGGACAGCCTGATCGCCGCGGTCCGGCTCGCCGCCGAC
ATCGCTCGTCGCCGCCCGGC

Downstream 100 bases:

>100_bases
AGCCTGTTCGATGAACCACTGTGACGGTCCGCGCGTTGACTTGCAGCCGTCGCACCGTATGTTCCGCGCGCGCGGCGATG
TCCGCCTGCGCCGCAGCGTT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSLETLPTLREALEAHGLWAKKAFGQHFLLDLNITRKIARLAQVGDGDVVIEVGPGPGGLTRALLETGARVIAVEKDERF
RPLLQEVADAAPHLTLVFGDALTADEAALSAGRPAHLVSNLPYNVGTPLLIKWLTGPWTPASLTLMFQKEVADRITAAPG
EDAYGRLAVIAQATADARPVMDVPARAFTPPPKVESAVVRLEPRAARPSPERLDALQKVTAAAFGQRRKMLRSSLKALGG
EPLITAAGLDPAARAEVVPVAGFLALADAWLARREAPATAAPRGR

Sequences:

>Translated_285_residues
MSLETLPTLREALEAHGLWAKKAFGQHFLLDLNITRKIARLAQVGDGDVVIEVGPGPGGLTRALLETGARVIAVEKDERF
RPLLQEVADAAPHLTLVFGDALTADEAALSAGRPAHLVSNLPYNVGTPLLIKWLTGPWTPASLTLMFQKEVADRITAAPG
EDAYGRLAVIAQATADARPVMDVPARAFTPPPKVESAVVRLEPRAARPSPERLDALQKVTAAAFGQRRKMLRSSLKALGG
EPLITAAGLDPAARAEVVPVAGFLALADAWLARREAPATAAPRGR
>Mature_284_residues
SLETLPTLREALEAHGLWAKKAFGQHFLLDLNITRKIARLAQVGDGDVVIEVGPGPGGLTRALLETGARVIAVEKDERFR
PLLQEVADAAPHLTLVFGDALTADEAALSAGRPAHLVSNLPYNVGTPLLIKWLTGPWTPASLTLMFQKEVADRITAAPGE
DAYGRLAVIAQATADARPVMDVPARAFTPPPKVESAVVRLEPRAARPSPERLDALQKVTAAAFGQRRKMLRSSLKALGGE
PLITAAGLDPAARAEVVPVAGFLALADAWLARREAPATAAPRGR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=293, Percent_Identity=38.5665529010239, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI7657198, Length=236, Percent_Identity=35.1694915254237, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=39.6946564885496, Blast_Score=184, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI25141369, Length=298, Percent_Identity=35.2348993288591, Blast_Score=164, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI25146882, Length=210, Percent_Identity=34.7619047619048, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324989, Length=189, Percent_Identity=37.5661375661376, Blast_Score=119, Evalue=4e-28,
Organism=Drosophila melanogaster, GI21357273, Length=297, Percent_Identity=33.6700336700337, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI21358017, Length=281, Percent_Identity=32.7402135231317, Blast_Score=132, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PHEZH (B4RBS4)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130550.1
- GeneID:   6791223
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1710
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30230; Mature: 30099

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 27-27 BINDING 29-29 BINDING 54-54 BINDING 75-75 BINDING 100-100 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLETLPTLREALEAHGLWAKKAFGQHFLLDLNITRKIARLAQVGDGDVVIEVGPGPGGL
CCCCHHHHHHHHHHHCCCHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCHH
TRALLETGARVIAVEKDERFRPLLQEVADAAPHLTLVFGDALTADEAALSAGRPAHLVSN
HHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCHHHHHC
LPYNVGTPLLIKWLTGPWTPASLTLMFQKEVADRITAAPGEDAYGRLAVIAQATADARPV
CCCCCCCCEEEEECCCCCCCHHEEEEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
MDVPARAFTPPPKVESAVVRLEPRAARPSPERLDALQKVTAAAFGQRRKMLRSSLKALGG
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPLITAAGLDPAARAEVVPVAGFLALADAWLARREAPATAAPRGR
CCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SLETLPTLREALEAHGLWAKKAFGQHFLLDLNITRKIARLAQVGDGDVVIEVGPGPGGL
CCCHHHHHHHHHHHCCCHHHHHCCCEEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCHH
TRALLETGARVIAVEKDERFRPLLQEVADAAPHLTLVFGDALTADEAALSAGRPAHLVSN
HHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCHHHHHC
LPYNVGTPLLIKWLTGPWTPASLTLMFQKEVADRITAAPGEDAYGRLAVIAQATADARPV
CCCCCCCCEEEEECCCCCCCHHEEEEEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCC
MDVPARAFTPPPKVESAVVRLEPRAARPSPERLDALQKVTAAAFGQRRKMLRSSLKALGG
CCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPLITAAGLDPAARAEVVPVAGFLALADAWLARREAPATAAPRGR
CCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA