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The map label for this gene is ygfK [H]

Identifier: 197105161

GI number: 197105161

Start: 1903717

End: 1905507

Strand: Reverse

Name: ygfK [H]

Synonym: PHZ_c1698

Alternate gene names: 197105161

Gene position: 1905507-1903717 (Counterclockwise)

Preceding gene: 197105163

Following gene: 197105160

Centisome position: 47.68

GC content: 70.58

Gene sequence:

>1791_bases
TTGCAGCGCACGCGTACCGAGGGGCCCGAGTACTTCCACAAGGTGGTCGACTGCCAGTGGGCCTGTCCGGCGCACACGCC
GGTGCCCGAGTACATCCGGCTGATCGCCGAGGGGCGGTACGCCGACGCCTACATGATCAACTGGCGGTCCAACGTCTTTC
CCGGCGTGCTGGGGCGGACCTGCGACCGGCCCTGCGAGCCCGCCTGCCGTCGCGGCCGGGTCGAGGAGGAGCCGGTGGCG
ATCTGCCGGCTCAAGCGCGTGGCGGCCGACAACAAGGGCGACGTGCTGGCGCGGATGCCCGAGCCGGCGCTGGCCCGCAA
CGGCCGGCGGGTGGCCCTGGTGGGGGCCGGCCCCGCCTCCCTGACGGTGGCCAGGGACCTCGCGACCCTGGGCTACGAGC
TGGTGCTCTACGACGCCGAGGCCCGGCCGGGCGGCTTCATCCGCAGCCAGATTCCCCGTTTCCGCCTGCCGGAGGAGGTG
ATCGACGAGGAGGTGGGCTACGTCACCCGTCTGGGCGGCGTGGAGCTGCGGCTGGGCCAGCCGGTGGACAGCCTGAAGGC
CCTGCTGGGCGAGGGATACGACGCGGTGTTCGTCGGCTCGGGCGCGCCGCGCGGCCGGGACCTGGACCTTCCGGGCCGGC
AGGAGGCCGCGGCCAACATCCACATCGGCATCGACTGGCTGAGCTCGGTCTCGTTCGGCCACATCGAGAAGATCGGCCGG
CGGGTCATCGTGCTGGGCGGCGGCAACACGGCCATGGACTGCTGCCGCACCTCCCGGCGCCTGGGCGGCCAGGACGTGAA
GGTGATCGTCCGCTCCGGCTTCGACGAGATGAAGGCCTCGCCCTGGGAGAAGGAGGACGCGATCCACGAGGACATCCCGA
TCCTCAACTATCGCGTCCCGAAGGAATTCACCCACGAGAACGGCCGCCTGACCGGCGTCGTCTTCGAGGTCGTCGAGGCC
CGATACGACGACATGGGCCGGCGCAAGCTGGTTCCCTCGGGCGCGCCGGACGAGCACCACGAATGCGACGACGTGCTGAT
CGCCGTGGGCCAGGAGAACGCCTTCCCCTGGATCGAGCGCGACATCGGCCTGGAGTTCGACGAGTGGGGCCTGCCGAAGG
TGGACCCCGCGACCATGCGCTCGACCATCCCCAACGTCTTCTTCGGCGGCGATGCGGCCTTCGGGCCGAAGAACATCATC
TGGGCCGTGGCGCACGGCCACGAGGCGGCGGTCTCGATCCACCGGCTGTGCGAGGGGCAGGACGTGGCCGAGCGGCCGCC
GCCGCACGCCAGCCTGGTCAGCCAGAAGATGGGCATCCACGAGTGGAGCTACGACAACGACGTCTCCAACGACCGCCGCT
ACCGCGTGCCGATGCGCGAGAAGGCCGAGGCGCTGACGAACGTGCGGCTGGAGGTCGAGCTGGGCTTCGACCGCGACCTG
GCCCTGCGCGAGGCCGAGCGGTGCCTGAACTGCGACGTGCAGACGGTGTTCGCCGACAAGCTGTGCATCGAGTGCGACGC
CTGCGTCGACATCTGTCCGATGGACTGCATCACCTTCACCGAGGACGGGGAGGAGGATGAGCTGCGCGGCCGGCTCAAGG
CGCCCGCGCTGAACCCCGAGCAGGACCTCTACGCCTCGGGCCGGCTGGGCACCGGGCGGATCATGGTCAAGGACGAGGAC
GTCTGCCTGCACTGCGGCCTGTGCGCCGAGCGGTGCCCGACCGGCGCCTGGGACATGCAGAAGTTCTATCTCGAGACCAC
CCAGGCGGAGGGCGCATGCCGGAGCCGGTGA

Upstream 100 bases:

>100_bases
CCTTCCGCGATCAACGCCGCGCCGTGGCCGCGGAGGCGCCCTGCCGGGCAGGGGACTTCGCCGCCTCGGGAGTAGAGCGT
CCCGGGAAGGAAGCGAAGTC

Downstream 100 bases:

>100_bases
CGAGCCAGGCCGCCCAGCCGCCAATCAGGCCCCGCGTGAACGACTTCGTCGTCAAGTTCGCCAACGTGAACGGGTCGGGA
TCGGCCAGCGCCAACGGGCT

Product: glutamate synthase (NADPH) small chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MQRTRTEGPEYFHKVVDCQWACPAHTPVPEYIRLIAEGRYADAYMINWRSNVFPGVLGRTCDRPCEPACRRGRVEEEPVA
ICRLKRVAADNKGDVLARMPEPALARNGRRVALVGAGPASLTVARDLATLGYELVLYDAEARPGGFIRSQIPRFRLPEEV
IDEEVGYVTRLGGVELRLGQPVDSLKALLGEGYDAVFVGSGAPRGRDLDLPGRQEAAANIHIGIDWLSSVSFGHIEKIGR
RVIVLGGGNTAMDCCRTSRRLGGQDVKVIVRSGFDEMKASPWEKEDAIHEDIPILNYRVPKEFTHENGRLTGVVFEVVEA
RYDDMGRRKLVPSGAPDEHHECDDVLIAVGQENAFPWIERDIGLEFDEWGLPKVDPATMRSTIPNVFFGGDAAFGPKNII
WAVAHGHEAAVSIHRLCEGQDVAERPPPHASLVSQKMGIHEWSYDNDVSNDRRYRVPMREKAEALTNVRLEVELGFDRDL
ALREAERCLNCDVQTVFADKLCIECDACVDICPMDCITFTEDGEEDELRGRLKAPALNPEQDLYASGRLGTGRIMVKDED
VCLHCGLCAERCPTGAWDMQKFYLETTQAEGACRSR

Sequences:

>Translated_596_residues
MQRTRTEGPEYFHKVVDCQWACPAHTPVPEYIRLIAEGRYADAYMINWRSNVFPGVLGRTCDRPCEPACRRGRVEEEPVA
ICRLKRVAADNKGDVLARMPEPALARNGRRVALVGAGPASLTVARDLATLGYELVLYDAEARPGGFIRSQIPRFRLPEEV
IDEEVGYVTRLGGVELRLGQPVDSLKALLGEGYDAVFVGSGAPRGRDLDLPGRQEAAANIHIGIDWLSSVSFGHIEKIGR
RVIVLGGGNTAMDCCRTSRRLGGQDVKVIVRSGFDEMKASPWEKEDAIHEDIPILNYRVPKEFTHENGRLTGVVFEVVEA
RYDDMGRRKLVPSGAPDEHHECDDVLIAVGQENAFPWIERDIGLEFDEWGLPKVDPATMRSTIPNVFFGGDAAFGPKNII
WAVAHGHEAAVSIHRLCEGQDVAERPPPHASLVSQKMGIHEWSYDNDVSNDRRYRVPMREKAEALTNVRLEVELGFDRDL
ALREAERCLNCDVQTVFADKLCIECDACVDICPMDCITFTEDGEEDELRGRLKAPALNPEQDLYASGRLGTGRIMVKDED
VCLHCGLCAERCPTGAWDMQKFYLETTQAEGACRSR
>Mature_596_residues
MQRTRTEGPEYFHKVVDCQWACPAHTPVPEYIRLIAEGRYADAYMINWRSNVFPGVLGRTCDRPCEPACRRGRVEEEPVA
ICRLKRVAADNKGDVLARMPEPALARNGRRVALVGAGPASLTVARDLATLGYELVLYDAEARPGGFIRSQIPRFRLPEEV
IDEEVGYVTRLGGVELRLGQPVDSLKALLGEGYDAVFVGSGAPRGRDLDLPGRQEAAANIHIGIDWLSSVSFGHIEKIGR
RVIVLGGGNTAMDCCRTSRRLGGQDVKVIVRSGFDEMKASPWEKEDAIHEDIPILNYRVPKEFTHENGRLTGVVFEVVEA
RYDDMGRRKLVPSGAPDEHHECDDVLIAVGQENAFPWIERDIGLEFDEWGLPKVDPATMRSTIPNVFFGGDAAFGPKNII
WAVAHGHEAAVSIHRLCEGQDVAERPPPHASLVSQKMGIHEWSYDNDVSNDRRYRVPMREKAEALTNVRLEVELGFDRDL
ALREAERCLNCDVQTVFADKLCIECDACVDICPMDCITFTEDGEEDELRGRLKAPALNPEQDLYASGRLGTGRIMVKDED
VCLHCGLCAERCPTGAWDMQKFYLETTQAEGACRSR

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=443, Percent_Identity=29.1196388261851, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI1789243, Length=537, Percent_Identity=28.6778398510242, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1788811, Length=421, Percent_Identity=32.0665083135392, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI87082180, Length=422, Percent_Identity=30.5687203791469, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1789606, Length=423, Percent_Identity=30.2600472813239, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1788468, Length=416, Percent_Identity=29.3269230769231, Blast_Score=150, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71984108, Length=446, Percent_Identity=28.6995515695067, Blast_Score=141, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17570289, Length=444, Percent_Identity=26.5765765765766, Blast_Score=136, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320030, Length=427, Percent_Identity=25.9953161592506, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24665547, Length=426, Percent_Identity=27.9342723004695, Blast_Score=152, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24665543, Length=426, Percent_Identity=27.9342723004695, Blast_Score=152, Evalue=8e-37,
Organism=Drosophila melanogaster, GI28574881, Length=425, Percent_Identity=28, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24665539, Length=425, Percent_Identity=28, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640763, Length=425, Percent_Identity=28.9411764705882, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI18858217, Length=425, Percent_Identity=28.9411764705882, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR017701 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 66314; Mature: 66314

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00198 4FE4S_FERREDOXIN ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTRTEGPEYFHKVVDCQWACPAHTPVPEYIRLIAEGRYADAYMINWRSNVFPGVLGRT
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHC
CDRPCEPACRRGRVEEEPVAICRLKRVAADNKGDVLARMPEPALARNGRRVALVGAGPAS
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHCCCCEEEEEECCCCH
LTVARDLATLGYELVLYDAEARPGGFIRSQIPRFRLPEEVIDEEVGYVTRLGGVELRLGQ
HHHHHHHHHCCEEEEEEECCCCCCCCHHHCCCCCCCCHHHHHHHHCCEEECCCEEEEECC
PVDSLKALLGEGYDAVFVGSGAPRGRDLDLPGRQEAAANIHIGIDWLSSVSFGHIEKIGR
CHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHCCC
RVIVLGGGNTAMDCCRTSRRLGGQDVKVIVRSGFDEMKASPWEKEDAIHEDIPILNYRVP
EEEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCCHHHCCCCCCHHCCHHCCCCEEEEECC
KEFTHENGRLTGVVFEVVEARYDDMGRRKLVPSGAPDEHHECDDVLIAVGQENAFPWIER
HHHHCCCCCEEHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHH
DIGLEFDEWGLPKVDPATMRSTIPNVFFGGDAAFGPKNIIWAVAHGHEAAVSIHRLCEGQ
CCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCC
DVAERPPPHASLVSQKMGIHEWSYDNDVSNDRRYRVPMREKAEALTNVRLEVELGFDRDL
CHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCEEEEEEECCCCCH
ALREAERCLNCDVQTVFADKLCIECDACVDICPMDCITFTEDGEEDELRGRLKAPALNPE
HHHHHHHHHCCCHHHEEHHHHHEECCCHHCCCCCCEEEECCCCCHHHHHCCCCCCCCCCC
QDLYASGRLGTGRIMVKDEDVCLHCGLCAERCPTGAWDMQKFYLETTQAEGACRSR
HHHEECCCCCCCEEEEECCCEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQRTRTEGPEYFHKVVDCQWACPAHTPVPEYIRLIAEGRYADAYMINWRSNVFPGVLGRT
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHC
CDRPCEPACRRGRVEEEPVAICRLKRVAADNKGDVLARMPEPALARNGRRVALVGAGPAS
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHCCCCEEEEEECCCCH
LTVARDLATLGYELVLYDAEARPGGFIRSQIPRFRLPEEVIDEEVGYVTRLGGVELRLGQ
HHHHHHHHHCCEEEEEEECCCCCCCCHHHCCCCCCCCHHHHHHHHCCEEECCCEEEEECC
PVDSLKALLGEGYDAVFVGSGAPRGRDLDLPGRQEAAANIHIGIDWLSSVSFGHIEKIGR
CHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCHHHHHHCCC
RVIVLGGGNTAMDCCRTSRRLGGQDVKVIVRSGFDEMKASPWEKEDAIHEDIPILNYRVP
EEEEEECCCHHHHHHHHHHHCCCCCEEHHHHCCCHHHCCCCCCHHCCHHCCCCEEEEECC
KEFTHENGRLTGVVFEVVEARYDDMGRRKLVPSGAPDEHHECDDVLIAVGQENAFPWIER
HHHHCCCCCEEHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCEEEEECCCCCCCCHHH
DIGLEFDEWGLPKVDPATMRSTIPNVFFGGDAAFGPKNIIWAVAHGHEAAVSIHRLCEGQ
CCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCC
DVAERPPPHASLVSQKMGIHEWSYDNDVSNDRRYRVPMREKAEALTNVRLEVELGFDRDL
CHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCEEEEEEECCCCCH
ALREAERCLNCDVQTVFADKLCIECDACVDICPMDCITFTEDGEEDELRGRLKAPALNPE
HHHHHHHHHCCCHHHEEHHHHHEECCCHHCCCCCCEEEECCCCCHHHHHCCCCCCCCCCC
QDLYASGRLGTGRIMVKDEDVCLHCGLCAERCPTGAWDMQKFYLETTQAEGACRSR
HHHEECCCCCCCEEEEECCCEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]