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The map label for this gene is hemB [H]

Identifier: 197105145

GI number: 197105145

Start: 1886586

End: 1887602

Strand: Direct

Name: hemB [H]

Synonym: PHZ_c1682

Alternate gene names: 197105145

Gene position: 1886586-1887602 (Clockwise)

Preceding gene: 197105139

Following gene: 197105146

Centisome position: 47.21

GC content: 71.88

Gene sequence:

>1017_bases
ATGACCCGCGCTCCCCTCGCCGCCTACCCCGCGCTCCGCCTGCGCCGCCTGCGCCAGGCCGACTGGATCCGCCGCCTCGT
CCGCGAGACCGTGCTCACGCCGGCCGACCTGATCTGGTCGATGGTCGTCCACGAGGGCGAGGGCGAGATCCGCGTGGCCT
CCATGCCGGGCGTGACCCGCCTGTCGGTGAAGGACGCCGCCCAGGCCGCGAAGGAAGCCAAGGCCCTCGGCATCCCGGCC
ATCGCCGTCTTCCCGCACATCGACGGCGCCAAGAAGGACGCCCGCGGCAGCCGGGCGGCCGATCCCAACGGCCTCGTCTG
CAACGCCGTCAAGGCGATGAAGGACGCCGCCCCCGAAGTGGGGATCATGTGCGACGTGGCGCTCGACCCCTTCACCGACC
ACGGCCACGACGGCCTGATCGAGGGCGGCCGCATCCTCAACGACGCGACCATCGAGGCCCTGGTCGCCCAGGCGCTGGTG
CAGGCGAAGGCCGGCTGCGACATCCTCGCCCCGTCCGACATGATGGACGGCCGCGTCGGGGCGCTGCGTAGCGCGCTGGA
GGCCGAGGGGCTGCAGGACGTGATGATCATGTCCTACGCCGCCAAGTACGCCTCGGCGTTCTACGGCCCCTACCGGGACG
CCATCGGCTCCGGAAAGCTGGGGACCGGCTCGGTGGACAACCCCGGCGACAAGAAGACCTACCAGATGGACCCCGCCAAC
ACGGACGAGGCCCTGCGCGAGGTGGCCCTCGACATCGCCGAGGGCGCCGACATGGTGATGGTGAAGCCCGGCCTGCCCTA
CCTCGACATCGTCCGCCGGGTGGTCGACGAGTTCGCCCTGCCGACCTTCGCGTTCCAGGTCTCGGGCGAGTACGCCATGC
TGATGGCGGCGGCCCAGAACGGGTGGCTGGACGAGGAACGCGCCATCCTGGAGAGCCTCGGCGCCTTCAAGCGGGCCGGC
GCCGCCGGGGTCATCACCTACTTCGCGCCCCGGGCGGCGCGCCTCCTGGGCGCGTGA

Upstream 100 bases:

>100_bases
TCGTAGCTCAGGGTCATGTTCTTGCCTCCCCTTTTGAGCGTCGCCTTAGCACGGCGGGAGGGTCTTGCCTTCCGGGGCCT
TCAGGGTCCAAAGCCCGGCC

Downstream 100 bases:

>100_bases
TCGTCGAGGTCGTCGGCGCGGTCGTCCGCGACGGCGCCGGCCGTCTGCTCGTGGTCCGCAAGCGCGGGACGCAGCGCTTC
ATGCTGCCGGGCGGCAAGCC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MTRAPLAAYPALRLRRLRQADWIRRLVRETVLTPADLIWSMVVHEGEGEIRVASMPGVTRLSVKDAAQAAKEAKALGIPA
IAVFPHIDGAKKDARGSRAADPNGLVCNAVKAMKDAAPEVGIMCDVALDPFTDHGHDGLIEGGRILNDATIEALVAQALV
QAKAGCDILAPSDMMDGRVGALRSALEAEGLQDVMIMSYAAKYASAFYGPYRDAIGSGKLGTGSVDNPGDKKTYQMDPAN
TDEALREVALDIAEGADMVMVKPGLPYLDIVRRVVDEFALPTFAFQVSGEYAMLMAAAQNGWLDEERAILESLGAFKRAG
AAGVITYFAPRAARLLGA

Sequences:

>Translated_338_residues
MTRAPLAAYPALRLRRLRQADWIRRLVRETVLTPADLIWSMVVHEGEGEIRVASMPGVTRLSVKDAAQAAKEAKALGIPA
IAVFPHIDGAKKDARGSRAADPNGLVCNAVKAMKDAAPEVGIMCDVALDPFTDHGHDGLIEGGRILNDATIEALVAQALV
QAKAGCDILAPSDMMDGRVGALRSALEAEGLQDVMIMSYAAKYASAFYGPYRDAIGSGKLGTGSVDNPGDKKTYQMDPAN
TDEALREVALDIAEGADMVMVKPGLPYLDIVRRVVDEFALPTFAFQVSGEYAMLMAAAQNGWLDEERAILESLGAFKRAG
AAGVITYFAPRAARLLGA
>Mature_337_residues
TRAPLAAYPALRLRRLRQADWIRRLVRETVLTPADLIWSMVVHEGEGEIRVASMPGVTRLSVKDAAQAAKEAKALGIPAI
AVFPHIDGAKKDARGSRAADPNGLVCNAVKAMKDAAPEVGIMCDVALDPFTDHGHDGLIEGGRILNDATIEALVAQALVQ
AKAGCDILAPSDMMDGRVGALRSALEAEGLQDVMIMSYAAKYASAFYGPYRDAIGSGKLGTGSVDNPGDKKTYQMDPANT
DEALREVALDIAEGADMVMVKPGLPYLDIVRRVVDEFALPTFAFQVSGEYAMLMAAAQNGWLDEERAILESLGAFKRAGA
AGVITYFAPRAARLLGA

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=42.443729903537, Blast_Score=228, Evalue=8e-60,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=44.375, Blast_Score=236, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=38.2165605095541, Blast_Score=187, Evalue=3e-48,
Organism=Drosophila melanogaster, GI21358291, Length=308, Percent_Identity=36.3636363636364, Blast_Score=211, Evalue=4e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35954; Mature: 35823

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAPLAAYPALRLRRLRQADWIRRLVRETVLTPADLIWSMVVHEGEGEIRVASMPGVTR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCEE
LSVKDAAQAAKEAKALGIPAIAVFPHIDGAKKDARGSRAADPNGLVCNAVKAMKDAAPEV
EHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GIMCDVALDPFTDHGHDGLIEGGRILNDATIEALVAQALVQAKAGCDILAPSDMMDGRVG
CEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEECCCHHCCCHHH
ALRSALEAEGLQDVMIMSYAAKYASAFYGPYRDAIGSGKLGTGSVDNPGDKKTYQMDPAN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCC
TDEALREVALDIAEGADMVMVKPGLPYLDIVRRVVDEFALPTFAFQVSGEYAMLMAAAQN
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHCC
GWLDEERAILESLGAFKRAGAAGVITYFAPRAARLLGA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TRAPLAAYPALRLRRLRQADWIRRLVRETVLTPADLIWSMVVHEGEGEIRVASMPGVTR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCEE
LSVKDAAQAAKEAKALGIPAIAVFPHIDGAKKDARGSRAADPNGLVCNAVKAMKDAAPEV
EHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GIMCDVALDPFTDHGHDGLIEGGRILNDATIEALVAQALVQAKAGCDILAPSDMMDGRVG
CEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCEECCCHHCCCHHH
ALRSALEAEGLQDVMIMSYAAKYASAFYGPYRDAIGSGKLGTGSVDNPGDKKTYQMDPAN
HHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCC
TDEALREVALDIAEGADMVMVKPGLPYLDIVRRVVDEFALPTFAFQVSGEYAMLMAAAQN
HHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHCC
GWLDEERAILESLGAFKRAGAAGVITYFAPRAARLLGA
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]