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The map label for this gene is glyA

Identifier: 197105138

GI number: 197105138

Start: 1879096

End: 1880385

Strand: Reverse

Name: glyA

Synonym: PHZ_c1675

Alternate gene names: 197105138

Gene position: 1880385-1879096 (Counterclockwise)

Preceding gene: 197105140

Following gene: 197105137

Centisome position: 47.05

GC content: 67.6

Gene sequence:

>1290_bases
GTGCACGTGCAATCGCAAGCGTCGTCGGACGATTTCTTCCTTCAGGGCGTGGGGTCGGCCGACCCGGCCGTGGCCGAGAT
CCTGGCGGGCGAGTTGAAGCGGCAGCAGGACCAGATCGAGCTGATCGCCTCGGAGAACATCGTCTCGAAGGCGGTGCTGG
ACGCCCAGGGCAGCGTGCTGACGAACAAGTACGCCGAGGGCTATCCCGGCAAGCGCTACTACGGCGGCTGCGAAGTCGTG
GACGAGGTCGAGCGCCTGGCCATCGAGCGGGCCAAGCAGCTGTTCGGCTGCGAACACGCCAACGTCCAGCCCCACTCGGG
CAGCCAGGCCAACCAGGCGGTGTTCATGGTCACCATGACGCCGGGCGACACCTTCATGGGCATGAACCTGGACCACGGCG
GCCACCTGACGCACGGCAAGAGCGTCAACCAGTCGGGCAAGTGGTTCAGCCCCGTGGCCTACGGCGTGCGCGCCCAGGAC
CACCTGATCGACTACGACGAGGCCTACGAGGTCGCCAAGGCGAACAACCCGAAGGTGATCATCGCCGGCGGCTCGGCCTA
TTCGCGCCACATCGACTTCAAGAAATTCCGCGAGATCGCCGACGAAGTGGGCGCGATCCTGATGTGCGACGTGGCCCACT
ATGCCGGCCTGATCGTGGCGGGCGAGTACCCGAACCCGTTCCCGCACGCCCACATCGTCACGACGACGACGCACAAGACC
CTTCGGGGTCCGCGCGGCGGCATGATCCTGACCAACGACAAGAAGCTGGCCAAGAAGATCGACAGCGCCGTCTTCCCGGG
CCTGCAGGGCGGCCCGCTCATGCACGTCATCGCCGCCAAGGCCGTGGCCTTCGGCGAGGCGCTGAAGCCCGAGTTCAAGC
AGTACGCCCGCCAGGTGATCGAGAACGCCCGCGCGCTGGCCGAGAGCCTGCAGAGCGTCGGCTTCAAGATCGTCTCCAAC
GGCACCGACAGCCACCTGATGCTGGTGGACCTGACGCCCAAGGGCGTCTCGGGCGCCGACGCCGAGATCGCGCTGGAGCG
GGCCGGGATCACCACCAACAAGAACTCGATCCCGGGCGATCCCCTGCCGCCGATGCAGACCTCCGGCCTGCGCGTCGGCA
CGCCGGCCGGCACCACCCGCGGCTTCGGCCCGGGCGAGTTCCGGCAGGTCGGCAAGTGGATCGGCGAGGTGCTGGATGCG
GTGGCGTCGGGCGAGGATCCGACGCCGGTCGAACAGAAGGTGCGCGGCGAGGTGCTGGCGCTGACGAAGCGCTTCCCCAT
CTACAGCTAA

Upstream 100 bases:

>100_bases
CCCGCGCGCCCGTGCGCGGTTGGAACCGGCGCGGCGAAGGAGTAAAGAACGCCCTCCGCCGGAAGACTCATCGCCAGCCC
AGAAAGGCCCGCCCTTGAAC

Downstream 100 bases:

>100_bases
TAGCGTTAAGTGGTCGGGAAGGGGGCTAGATATGCGCTGCCCGTTCTGCGGCCACGACGAGACCCAGGTGAAGGACAGCC
GCCCGTCGGAAGACGGCGCG

Product: serine hydroxymethyl transferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MHVQSQASSDDFFLQGVGSADPAVAEILAGELKRQQDQIELIASENIVSKAVLDAQGSVLTNKYAEGYPGKRYYGGCEVV
DEVERLAIERAKQLFGCEHANVQPHSGSQANQAVFMVTMTPGDTFMGMNLDHGGHLTHGKSVNQSGKWFSPVAYGVRAQD
HLIDYDEAYEVAKANNPKVIIAGGSAYSRHIDFKKFREIADEVGAILMCDVAHYAGLIVAGEYPNPFPHAHIVTTTTHKT
LRGPRGGMILTNDKKLAKKIDSAVFPGLQGGPLMHVIAAKAVAFGEALKPEFKQYARQVIENARALAESLQSVGFKIVSN
GTDSHLMLVDLTPKGVSGADAEIALERAGITTNKNSIPGDPLPPMQTSGLRVGTPAGTTRGFGPGEFRQVGKWIGEVLDA
VASGEDPTPVEQKVRGEVLALTKRFPIYS

Sequences:

>Translated_429_residues
MHVQSQASSDDFFLQGVGSADPAVAEILAGELKRQQDQIELIASENIVSKAVLDAQGSVLTNKYAEGYPGKRYYGGCEVV
DEVERLAIERAKQLFGCEHANVQPHSGSQANQAVFMVTMTPGDTFMGMNLDHGGHLTHGKSVNQSGKWFSPVAYGVRAQD
HLIDYDEAYEVAKANNPKVIIAGGSAYSRHIDFKKFREIADEVGAILMCDVAHYAGLIVAGEYPNPFPHAHIVTTTTHKT
LRGPRGGMILTNDKKLAKKIDSAVFPGLQGGPLMHVIAAKAVAFGEALKPEFKQYARQVIENARALAESLQSVGFKIVSN
GTDSHLMLVDLTPKGVSGADAEIALERAGITTNKNSIPGDPLPPMQTSGLRVGTPAGTTRGFGPGEFRQVGKWIGEVLDA
VASGEDPTPVEQKVRGEVLALTKRFPIYS
>Mature_429_residues
MHVQSQASSDDFFLQGVGSADPAVAEILAGELKRQQDQIELIASENIVSKAVLDAQGSVLTNKYAEGYPGKRYYGGCEVV
DEVERLAIERAKQLFGCEHANVQPHSGSQANQAVFMVTMTPGDTFMGMNLDHGGHLTHGKSVNQSGKWFSPVAYGVRAQD
HLIDYDEAYEVAKANNPKVIIAGGSAYSRHIDFKKFREIADEVGAILMCDVAHYAGLIVAGEYPNPFPHAHIVTTTTHKT
LRGPRGGMILTNDKKLAKKIDSAVFPGLQGGPLMHVIAAKAVAFGEALKPEFKQYARQVIENARALAESLQSVGFKIVSN
GTDSHLMLVDLTPKGVSGADAEIALERAGITTNKNSIPGDPLPPMQTSGLRVGTPAGTTRGFGPGEFRQVGKWIGEVLDA
VASGEDPTPVEQKVRGEVLALTKRFPIYS

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=405, Percent_Identity=48.1481481481481, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=405, Percent_Identity=48.1481481481481, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=405, Percent_Identity=48.1481481481481, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI19923315, Length=405, Percent_Identity=48.1481481481481, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=405, Percent_Identity=47.1604938271605, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI22547186, Length=411, Percent_Identity=47.9318734793187, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI22547189, Length=397, Percent_Identity=45.088161209068, Blast_Score=306, Evalue=2e-83,
Organism=Escherichia coli, GI1788902, Length=415, Percent_Identity=57.1084337349398, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI25144732, Length=411, Percent_Identity=47.4452554744526, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25144729, Length=411, Percent_Identity=47.4452554744526, Blast_Score=365, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=48.1296758104738, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=46.5174129353234, Blast_Score=345, Evalue=8e-96,
Organism=Drosophila melanogaster, GI24640005, Length=413, Percent_Identity=47.4576271186441, Blast_Score=367, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=413, Percent_Identity=47.4576271186441, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_PHEZH (B4RB35)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130515.1
- ProteinModelPortal:   B4RB35
- GeneID:   6791187
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1675
- HOGENOM:   HBG301263
- OMA:   IDMEQVA
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46004; Mature: 46004

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVQSQASSDDFFLQGVGSADPAVAEILAGELKRQQDQIELIASENIVSKAVLDAQGSVL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCH
TNKYAEGYPGKRYYGGCEVVDEVERLAIERAKQLFGCEHANVQPHSGSQANQAVFMVTMT
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEC
PGDTFMGMNLDHGGHLTHGKSVNQSGKWFSPVAYGVRAQDHLIDYDEAYEVAKANNPKVI
CCCEEEEECCCCCCCCCCCCCCCCCCCEECHHHHCCCHHHHCCCHHHHHHHHCCCCCEEE
IAGGSAYSRHIDFKKFREIADEVGAILMCDVAHYAGLIVAGEYPNPFPHAHIVTTTTHKT
EECCCHHHHCCCHHHHHHHHHHHCEEEEEEHHHHCCEEEECCCCCCCCCCEEEEECCHHH
LRGPRGGMILTNDKKLAKKIDSAVFPGLQGGPLMHVIAAKAVAFGEALKPEFKQYARQVI
HCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ENARALAESLQSVGFKIVSNGTDSHLMLVDLTPKGVSGADAEIALERAGITTNKNSIPGD
HHHHHHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCHHEEEEECCCCCCCCCCCCC
PLPPMQTSGLRVGTPAGTTRGFGPGEFRQVGKWIGEVLDAVASGEDPTPVEQKVRGEVLA
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHEE
LTKRFPIYS
HHHCCCCCC
>Mature Secondary Structure
MHVQSQASSDDFFLQGVGSADPAVAEILAGELKRQQDQIELIASENIVSKAVLDAQGSVL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCH
TNKYAEGYPGKRYYGGCEVVDEVERLAIERAKQLFGCEHANVQPHSGSQANQAVFMVTMT
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEC
PGDTFMGMNLDHGGHLTHGKSVNQSGKWFSPVAYGVRAQDHLIDYDEAYEVAKANNPKVI
CCCEEEEECCCCCCCCCCCCCCCCCCCEECHHHHCCCHHHHCCCHHHHHHHHCCCCCEEE
IAGGSAYSRHIDFKKFREIADEVGAILMCDVAHYAGLIVAGEYPNPFPHAHIVTTTTHKT
EECCCHHHHCCCHHHHHHHHHHHCEEEEEEHHHHCCEEEECCCCCCCCCCEEEEECCHHH
LRGPRGGMILTNDKKLAKKIDSAVFPGLQGGPLMHVIAAKAVAFGEALKPEFKQYARQVI
HCCCCCCEEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ENARALAESLQSVGFKIVSNGTDSHLMLVDLTPKGVSGADAEIALERAGITTNKNSIPGD
HHHHHHHHHHHHCCEEEEECCCCCEEEEEEECCCCCCCCCHHEEEEECCCCCCCCCCCCC
PLPPMQTSGLRVGTPAGTTRGFGPGEFRQVGKWIGEVLDAVASGEDPTPVEQKVRGEVLA
CCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHEE
LTKRFPIYS
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA