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The map label for this gene is rfaE [H]

Identifier: 197105088

GI number: 197105088

Start: 1832086

End: 1833555

Strand: Reverse

Name: rfaE [H]

Synonym: PHZ_c1625

Alternate gene names: 197105088

Gene position: 1833555-1832086 (Counterclockwise)

Preceding gene: 197105089

Following gene: 197105087

Centisome position: 45.88

GC content: 73.33

Gene sequence:

>1470_bases
ATGGATCTGGCCGCACTCCAGCACCTCCTCAGCGAGGTCCCGGGCAAGCGCGTCGCCTGCGTGGGCGACCTGATGGTGGA
CCGTTTCGTCTACGGCTCGGTCAGCCGCGTCTCGCCCGAGGCGCCCATCCCGGTGCTCGCCCGCAGCCGCGAGATCATGA
TGCTGGGCGCCGCCGGCAACGTGGCGCGCAACGTCGCCGCCCTGGGCGGCTCGGTCGCCCTGGTGGGGCTCGTCGGCGGC
GACGCCGAGGGCCATGAAGCCTCCCGCCTGGTCGGCGAGGAGCCGGCCATCGAGGGCTATCTCGTCACCGATCCCTCCCG
GCCGACCACCCTGAAGACCCGCTTTGTCTCGGGCGGCCAGCAGCTCCTGCGCGTGGACCTCGAGGAGAGCCGGCCGGTCG
AGGGCGAGTGCGAGCAGCGGCTCGTGCGCACCATCCGAGACGCCGCCCGGGGCGCCGGCGTGATCCTGCTGTCCGACTAC
GGCAAGGGCGTGGTGACCGACGCCGTCATCGCCGCCTGCCGCGAGGCCGCCGCCGAGACCGGCGCCAAGGTGGTGGTGGA
CTCCAAGGCCCGCTCGTTCGCCCGCTACGGCGAGATCGACCTCGTCAAACCGAACGCCTCCGAGCTGGCCTACGCCACCG
ACATGCCGACCGACACCGACGCCGAGGTGGAGGCCGCGCTCGCCCGCGCGCTCGAGCTGTGGTCGGCGCGGGGCGTTCTG
GTCACCCGCTCGGGCAAGGGCGCCTCCCTCGGCCTGCGCGGCTGGCCGGTGCGCCACTTCCCGACCGTGGCGCGCGAGGT
GTTCGACGCCTCGGGGGCGGGGGACACGGCCCTGGCGGCCCTCGGCTTGGCCCTGGCCGGCGGCGCGGGGCTGGAGGACG
CCATCGCCTTCGCCCAGCTCGCCTCCGGCGTCGCGGTGGGCAAGGCGGGCACCGCGACGGTCTCGCCCGAGGAGCTGGTG
GAGGCCGTCCTGACCGCCCACATGGCGCCGGCCGAGGCCAAGGTCGCCACGCCGCAGCGCATGGTCGAGGAGGTTGCCCG
CTGGCGCGCCCAGGGCCTGCGGGTGGGCTTCACCAACGGCTGCTTCGACATCCTGCACCGCGGCCACGTGGCCTACCTTT
CCCAGGCCAAGGCCTGGTGCGACCGGCTGATCGTGGGCCTGAATTCCGACGAGTCCGTCCGCGCCCTGAAGGGCGAGGGG
CGGCCGGTGAACGACCTGGAGAGCCGCGCCCTGGTGCTGGCCGGGCTGCAGAGCGTGGATCTCGTCGCGCCGTTCGAGGA
GCTGACGCCGCTGAAGCTGATCGAGGCCGCCCGGCCCGACGTGCTGGTCAAGGGCGCCGACTATTCCGAGGACCAGGTGG
TGGGCGCCGAGCAGGTCCGGTCCTGGGGCGGCGAGGTGAAGCTCGCCAGCTTGGTGGACGGCTATTCCACCACCGCGGCC
ATCGCCCGGATGAAGAAAGAGAAGGCATGA

Upstream 100 bases:

>100_bases
CTGGACGGCGCCGAGCCGCTGCTGCGCCAGCTCAAGGCGCTGCACGAGGTCGCCCGCGCGGCTTGACCGGCCCGCCAACG
CGCCGCACACCCTGAGCCCC

Downstream 100 bases:

>100_bases
CCCGCAAGATCGTCTTCGTGACCGGCGGCGCGGGCTTCATCGGCTCGAACATCGCCGCCAAGCTGGCCGAGGACCGCAGC
CTGGACGTCGTCGTCTGCGA

Product: rfaE protein

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MDLAALQHLLSEVPGKRVACVGDLMVDRFVYGSVSRVSPEAPIPVLARSREIMMLGAAGNVARNVAALGGSVALVGLVGG
DAEGHEASRLVGEEPAIEGYLVTDPSRPTTLKTRFVSGGQQLLRVDLEESRPVEGECEQRLVRTIRDAARGAGVILLSDY
GKGVVTDAVIAACREAAAETGAKVVVDSKARSFARYGEIDLVKPNASELAYATDMPTDTDAEVEAALARALELWSARGVL
VTRSGKGASLGLRGWPVRHFPTVAREVFDASGAGDTALAALGLALAGGAGLEDAIAFAQLASGVAVGKAGTATVSPEELV
EAVLTAHMAPAEAKVATPQRMVEEVARWRAQGLRVGFTNGCFDILHRGHVAYLSQAKAWCDRLIVGLNSDESVRALKGEG
RPVNDLESRALVLAGLQSVDLVAPFEELTPLKLIEAARPDVLVKGADYSEDQVVGAEQVRSWGGEVKLASLVDGYSTTAA
IARMKKEKA

Sequences:

>Translated_489_residues
MDLAALQHLLSEVPGKRVACVGDLMVDRFVYGSVSRVSPEAPIPVLARSREIMMLGAAGNVARNVAALGGSVALVGLVGG
DAEGHEASRLVGEEPAIEGYLVTDPSRPTTLKTRFVSGGQQLLRVDLEESRPVEGECEQRLVRTIRDAARGAGVILLSDY
GKGVVTDAVIAACREAAAETGAKVVVDSKARSFARYGEIDLVKPNASELAYATDMPTDTDAEVEAALARALELWSARGVL
VTRSGKGASLGLRGWPVRHFPTVAREVFDASGAGDTALAALGLALAGGAGLEDAIAFAQLASGVAVGKAGTATVSPEELV
EAVLTAHMAPAEAKVATPQRMVEEVARWRAQGLRVGFTNGCFDILHRGHVAYLSQAKAWCDRLIVGLNSDESVRALKGEG
RPVNDLESRALVLAGLQSVDLVAPFEELTPLKLIEAARPDVLVKGADYSEDQVVGAEQVRSWGGEVKLASLVDGYSTTAA
IARMKKEKA
>Mature_489_residues
MDLAALQHLLSEVPGKRVACVGDLMVDRFVYGSVSRVSPEAPIPVLARSREIMMLGAAGNVARNVAALGGSVALVGLVGG
DAEGHEASRLVGEEPAIEGYLVTDPSRPTTLKTRFVSGGQQLLRVDLEESRPVEGECEQRLVRTIRDAARGAGVILLSDY
GKGVVTDAVIAACREAAAETGAKVVVDSKARSFARYGEIDLVKPNASELAYATDMPTDTDAEVEAALARALELWSARGVL
VTRSGKGASLGLRGWPVRHFPTVAREVFDASGAGDTALAALGLALAGGAGLEDAIAFAQLASGVAVGKAGTATVSPEELV
EAVLTAHMAPAEAKVATPQRMVEEVARWRAQGLRVGFTNGCFDILHRGHVAYLSQAKAWCDRLIVGLNSDESVRALKGEG
RPVNDLESRALVLAGLQSVDLVAPFEELTPLKLIEAARPDVLVKGADYSEDQVVGAEQVRSWGGEVKLASLVDGYSTTAA
IARMKKEKA

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=471, Percent_Identity=42.2505307855626, Blast_Score=320, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 51247; Mature: 51247

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLAALQHLLSEVPGKRVACVGDLMVDRFVYGSVSRVSPEAPIPVLARSREIMMLGAAGN
CCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCHHHCCCCCCCCHHCCCCEEEEEECCCH
VARNVAALGGSVALVGLVGGDAEGHEASRLVGEEPAIEGYLVTDPSRPTTLKTRFVSGGQ
HHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCEEEECCCCCCEEHHHHHHCCH
QLLRVDLEESRPVEGECEQRLVRTIRDAARGAGVILLSDYGKGVVTDAVIAACREAAAET
HHEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
GAKVVVDSKARSFARYGEIDLVKPNASELAYATDMPTDTDAEVEAALARALELWSARGVL
CCEEEECCHHHHHHHCCCEEEECCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
VTRSGKGASLGLRGWPVRHFPTVAREVFDASGAGDTALAALGLALAGGAGLEDAIAFAQL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
ASGVAVGKAGTATVSPEELVEAVLTAHMAPAEAKVATPQRMVEEVARWRAQGLRVGFTNG
HCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCH
CFDILHRGHVAYLSQAKAWCDRLIVGLNSDESVRALKGEGRPVNDLESRALVLAGLQSVD
HHHHHHCCHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCC
LVAPFEELTPLKLIEAARPDVLVKGADYSEDQVVGAEQVRSWGGEVKLASLVDGYSTTAA
CCCCHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEEHHHCCCHHHHH
IARMKKEKA
HHHHHHHCC
>Mature Secondary Structure
MDLAALQHLLSEVPGKRVACVGDLMVDRFVYGSVSRVSPEAPIPVLARSREIMMLGAAGN
CCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCHHHCCCCCCCCHHCCCCEEEEEECCCH
VARNVAALGGSVALVGLVGGDAEGHEASRLVGEEPAIEGYLVTDPSRPTTLKTRFVSGGQ
HHHHHHHCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCEEEECCCCCCEEHHHHHHCCH
QLLRVDLEESRPVEGECEQRLVRTIRDAARGAGVILLSDYGKGVVTDAVIAACREAAAET
HHEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
GAKVVVDSKARSFARYGEIDLVKPNASELAYATDMPTDTDAEVEAALARALELWSARGVL
CCEEEECCHHHHHHHCCCEEEECCCHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
VTRSGKGASLGLRGWPVRHFPTVAREVFDASGAGDTALAALGLALAGGAGLEDAIAFAQL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
ASGVAVGKAGTATVSPEELVEAVLTAHMAPAEAKVATPQRMVEEVARWRAQGLRVGFTNG
HCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCH
CFDILHRGHVAYLSQAKAWCDRLIVGLNSDESVRALKGEGRPVNDLESRALVLAGLQSVD
HHHHHHCCHHHHHHHHHHHHHHHEECCCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCC
LVAPFEELTPLKLIEAARPDVLVKGADYSEDQVVGAEQVRSWGGEVKLASLVDGYSTTAA
CCCCHHHCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEEHHHCCCHHHHH
IARMKKEKA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA