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The map label for this gene is panB

Identifier: 197104996

GI number: 197104996

Start: 1733171

End: 1733998

Strand: Direct

Name: panB

Synonym: PHZ_c1533

Alternate gene names: 197104996

Gene position: 1733171-1733998 (Clockwise)

Preceding gene: 197104995

Following gene: 197104997

Centisome position: 43.37

GC content: 71.01

Gene sequence:

>828_bases
TTGTCCAGCCACCGGGAAGAGAAGGTCCGGCGCATCGCCGCCCCGGACATCGCTGCGCGCAAGGGCGGCACGCCGATCGT
GTGCCTCACTGCCTACACCGCGCCGGTGGCCGAGCTCCTGGACGAGCACTGCGACCTGCTGCTGGTGGGCGACAGCGTGG
GCATGGTGGTCCACGGGCTTCCGAACACCGTCGGCGTGACGCTCGACATGATGATCCTGCACGGCCAGGCGGTGATGCGC
GGCTCGCGCCGCGCCATGGTCGTCGTGGACATGCCGTTCGGCTCGTACGAGGGCGCCCCCGAGACCGCCTACGACAACGC
CGCCCGCCTCATGAAGGAGACGGGCGCGCAGGCCGTGAAGGTCGAGTCCGGTCCGACCGTCATCGACACCATCCAGTACC
TGGTGAAGCGCGGCATCCCGGTCATGGGGCACGTCGGCCTGCGGCCCCAGGCGGTTCTGGTGGACGGCGGCTTCAAGGCC
AAGGGCAAGGCGGGCGAGGAGCGCCGCCGCATCCTGGAGGAGGCGAGGGCGACCGCCGACGCCGGCGCGTTCGCGGTGGT
GGTCGAGGGCGTGGCCGAGGGCCTGGCCCGGGAGATCACCGAGGCCATCGAGGTTCCGACCATCGGCATCGGCGCGTCCG
CGGGCTGCGACGGGCAGATCCTGGTCACGGACGACATGCTGGGCCTCTTCGACTGGACCCCGAAGTTCGTGCGCCGTTAC
GGCGACCTGCGGGGCGAGATCGGCAAGGCCGTGGCGGCCTACGCAGAGGATGTCCGGGCCCGTCGCTTCCCGGGACCAGC
CGAGATCTACTTCGCCAAGGCGGGGTAG

Upstream 100 bases:

>100_bases
CTCGGCCCTGTCGGGCGGCTTCGGCGTCTGGGTCGCGGTCGGCCTGGCCGTGCTGCTGCTGGGCTTCGCCCTGTCGCGCG
TCATCGAGGAGATCTGAGGC

Downstream 100 bases:

>100_bases
CGTTGCGGCTGAGGGCGGCGCGCTATAGGTTCCCGGCCTCTGCCCCAAGGGCCAGCTAAAGAGTCCGGAGTCCCCTTCAC
GTGACGGATCTGTTCGAAGA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSSHREEKVRRIAAPDIAARKGGTPIVCLTAYTAPVAELLDEHCDLLLVGDSVGMVVHGLPNTVGVTLDMMILHGQAVMR
GSRRAMVVVDMPFGSYEGAPETAYDNAARLMKETGAQAVKVESGPTVIDTIQYLVKRGIPVMGHVGLRPQAVLVDGGFKA
KGKAGEERRRILEEARATADAGAFAVVVEGVAEGLAREITEAIEVPTIGIGASAGCDGQILVTDDMLGLFDWTPKFVRRY
GDLRGEIGKAVAAYAEDVRARRFPGPAEIYFAKAG

Sequences:

>Translated_275_residues
MSSHREEKVRRIAAPDIAARKGGTPIVCLTAYTAPVAELLDEHCDLLLVGDSVGMVVHGLPNTVGVTLDMMILHGQAVMR
GSRRAMVVVDMPFGSYEGAPETAYDNAARLMKETGAQAVKVESGPTVIDTIQYLVKRGIPVMGHVGLRPQAVLVDGGFKA
KGKAGEERRRILEEARATADAGAFAVVVEGVAEGLAREITEAIEVPTIGIGASAGCDGQILVTDDMLGLFDWTPKFVRRY
GDLRGEIGKAVAAYAEDVRARRFPGPAEIYFAKAG
>Mature_274_residues
SSHREEKVRRIAAPDIAARKGGTPIVCLTAYTAPVAELLDEHCDLLLVGDSVGMVVHGLPNTVGVTLDMMILHGQAVMRG
SRRAMVVVDMPFGSYEGAPETAYDNAARLMKETGAQAVKVESGPTVIDTIQYLVKRGIPVMGHVGLRPQAVLVDGGFKAK
GKAGEERRRILEEARATADAGAFAVVVEGVAEGLAREITEAIEVPTIGIGASAGCDGQILVTDDMLGLFDWTPKFVRRYG
DLRGEIGKAVAAYAEDVRARRFPGPAEIYFAKAG

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=239, Percent_Identity=41.4225941422594, Blast_Score=170, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319653, Length=251, Percent_Identity=38.2470119521912, Blast_Score=163, Evalue=3e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PHEZH (B4RAD7)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130373.1
- GeneID:   6791040
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1533
- HOGENOM:   HBG299908
- OMA:   DMMIAHG
- ProtClustDB:   PRK00311
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 29231; Mature: 29100

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: ACT_SITE 189-189 BINDING 90-90 BINDING 120-120

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHREEKVRRIAAPDIAARKGGTPIVCLTAYTAPVAELLDEHCDLLLVGDSVGMVVHGL
CCCHHHHHHHHHCCCCHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCCEEEECC
PNTVGVTLDMMILHGQAVMRGSRRAMVVVDMPFGSYEGAPETAYDNAARLMKETGAQAVK
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
VESGPTVIDTIQYLVKRGIPVMGHVGLRPQAVLVDGGFKAKGKAGEERRRILEEARATAD
ECCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
AGAFAVVVEGVAEGLAREITEAIEVPTIGIGASAGCDGQILVTDDMLGLFDWTPKFVRRY
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHCCCHHHHHHH
GDLRGEIGKAVAAYAEDVRARRFPGPAEIYFAKAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
SSHREEKVRRIAAPDIAARKGGTPIVCLTAYTAPVAELLDEHCDLLLVGDSVGMVVHGL
CCHHHHHHHHHCCCCHHHHCCCCCEEEEECCHHHHHHHHHHCCCEEEECCCCCEEEECC
PNTVGVTLDMMILHGQAVMRGSRRAMVVVDMPFGSYEGAPETAYDNAARLMKETGAQAVK
CCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEE
VESGPTVIDTIQYLVKRGIPVMGHVGLRPQAVLVDGGFKAKGKAGEERRRILEEARATAD
ECCCCCHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCC
AGAFAVVVEGVAEGLAREITEAIEVPTIGIGASAGCDGQILVTDDMLGLFDWTPKFVRRY
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCHHHHCCCHHHHHHH
GDLRGEIGKAVAAYAEDVRARRFPGPAEIYFAKAG
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA