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The map label for this gene is slt [H]

Identifier: 197104820

GI number: 197104820

Start: 1534450

End: 1536057

Strand: Reverse

Name: slt [H]

Synonym: PHZ_c1354

Alternate gene names: 197104820

Gene position: 1536057-1534450 (Counterclockwise)

Preceding gene: 197104821

Following gene: 197104818

Centisome position: 38.44

GC content: 73.01

Gene sequence:

>1608_bases
ATGACGCCCATAAAGCGCCTGTGCATGGGGGCCGCCGCCCTCTCGATCCTTGCAGCCTCCGCAGGCGCCGCCGCGGCCGA
ATCGCCGAGGCCGCTCTCGGACCGCGACGCGCGGGCCTACGCCGCCGCCTTCGCCGCCGCCGACCAGGGCGACTTCGTGG
GCGCTCAGATCGCCGCCGCGGGGATCAAGGACAATTCCCTGCTGGGCTACCTGTCCTTCCGCCAGCTGATGCACCCGACC
GCGCACAAGGCCAGCTTCGAGGAGCTGTCCGGCTGGCTGTCGAAGTTCCGGGACCTGCCGCTGGCCGACCGCGTCTTTTC
GCTCGCCGCCAAGCGCAAGCCCAGCGGCGCCGAGGCCCCGCCTGCGCCGGACGTCGGCCCCGACCCCAACGCCGAGTCGG
CCCGCAAGGCGCGCGAGGCCTACTATTCCGGCGAGCCGCAGAAGGCCTTCCTGCTGGCCGCGGCTGCCGGCGAGCGCTGG
ATCGCGGGCCTTTCGGCCTATCGGCTGAAGAACTTTCCCGAGGCCGAGGTCCACTTCGCCGACCTTGCTCGCGACGAGGA
CGAGGATTCCTGGCTGCGCTCGGCCGCGGCTTTCTGGGCCGCCAGGTCGGCGGAGGCCCTGGGCGATGCGGTGACGGCCC
AGACCTTCCTGCGGCTCGCGGCGCAGGCGCCGGAGACCTTCTACGGCATGATCGCCGAGCGGCGGGTGCAGCTCGTCCAG
GCGCAGGCGCCGACCGGCCAGATCGTGCTGGCCAGCTATCGCGCGCCGCCGCCGGCGCCGAAGGCCGATCCCGCGCACCT
GCTGGCCTCGCAGCCGCGGGCCCACCGCGCCGCCGCGCTCGTCCAGATCGGCCGGTTCGAGGAGGCCCGCCAGGAGATCC
GCGCCGGCCTCGCCGCCGCCGCCCCCGACGAGCGCCGCGCCTGGAACCGGCTTGCGGCCGACCTGCGCCTGGGCCGGGCT
GCGGCCGAGAAGCCGACGCCGGGCTATCGGATCCTCGGCAGCGACTATCCCGTGCCGGCGCTGGAGCCGAAATGGGGTTT
CACCGTCGACCGGGCCCTGGTCTACGCCCTGGTCTGGCAGGAGAGCCGGTTCAATCCGCAGGCCGTCTCGCCGGCGGGCG
CTATCGGCCTGATGCAGCTGATGCCCGAGACGGCCGCCCGGGCCGCCGGGGACGACAAGCTGAAGAAGGACGCCAGCCCG
CTCTTCGACCCGGCATTCAACCTGCGGGTCGGGCAGGACTACCTCACCTGGCTGATGGAACGCGGCGTCGGCTACGACCT
GCTGCGGACGGTGGCGGCGTATAACGGCGGGCCGGGTGCGGTGATGAAGGCCGCCGCCAACGCGGGCGACGACGATCCCC
TGCTGCTGATCGAGTCGCTGCCGGCCCAGGAAACCCGCGCCTACGTCGAGAAGGTGCTGGCCGCCTACTGGACCTACAAG
CGCATGTTCGGCGAGGAGGCGCGGTCCCTCGACGCCCTCGCCTCGGGCGCCCGGACGATCGACGCGCGGCTCGACCTACC
CGATGCGCGGGGGCCGCAGCCCGAGCTTACGGCGAAGACGATCCAGGTCGGGCTCCTGAAGGCTGACTACGCGGGCGCGC
TCGACTAG

Upstream 100 bases:

>100_bases
GATCGTCCCGACCGCCCTGCCTAGCGTGGCTGCTTCGGTCTCGGGATAAGGGTCCCGCGGGCAGGCTGCAGAAGACGGCC
GTCCCCGCGGCGGAGTTGGC

Downstream 100 bases:

>100_bases
CACCCCGAACGGCGCGGAGCTGAACAGGCCGCCGCGGACCAGTCCCACGACCACCGCCCCCGGCCCGCCCGCCGCGACGT
GCGCGCCCAGCGCCTCGACC

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTPIKRLCMGAAALSILAASAGAAAAESPRPLSDRDARAYAAAFAAADQGDFVGAQIAAAGIKDNSLLGYLSFRQLMHPT
AHKASFEELSGWLSKFRDLPLADRVFSLAAKRKPSGAEAPPAPDVGPDPNAESARKAREAYYSGEPQKAFLLAAAAGERW
IAGLSAYRLKNFPEAEVHFADLARDEDEDSWLRSAAAFWAARSAEALGDAVTAQTFLRLAAQAPETFYGMIAERRVQLVQ
AQAPTGQIVLASYRAPPPAPKADPAHLLASQPRAHRAAALVQIGRFEEARQEIRAGLAAAAPDERRAWNRLAADLRLGRA
AAEKPTPGYRILGSDYPVPALEPKWGFTVDRALVYALVWQESRFNPQAVSPAGAIGLMQLMPETAARAAGDDKLKKDASP
LFDPAFNLRVGQDYLTWLMERGVGYDLLRTVAAYNGGPGAVMKAAANAGDDDPLLLIESLPAQETRAYVEKVLAAYWTYK
RMFGEEARSLDALASGARTIDARLDLPDARGPQPELTAKTIQVGLLKADYAGALD

Sequences:

>Translated_535_residues
MTPIKRLCMGAAALSILAASAGAAAAESPRPLSDRDARAYAAAFAAADQGDFVGAQIAAAGIKDNSLLGYLSFRQLMHPT
AHKASFEELSGWLSKFRDLPLADRVFSLAAKRKPSGAEAPPAPDVGPDPNAESARKAREAYYSGEPQKAFLLAAAAGERW
IAGLSAYRLKNFPEAEVHFADLARDEDEDSWLRSAAAFWAARSAEALGDAVTAQTFLRLAAQAPETFYGMIAERRVQLVQ
AQAPTGQIVLASYRAPPPAPKADPAHLLASQPRAHRAAALVQIGRFEEARQEIRAGLAAAAPDERRAWNRLAADLRLGRA
AAEKPTPGYRILGSDYPVPALEPKWGFTVDRALVYALVWQESRFNPQAVSPAGAIGLMQLMPETAARAAGDDKLKKDASP
LFDPAFNLRVGQDYLTWLMERGVGYDLLRTVAAYNGGPGAVMKAAANAGDDDPLLLIESLPAQETRAYVEKVLAAYWTYK
RMFGEEARSLDALASGARTIDARLDLPDARGPQPELTAKTIQVGLLKADYAGALD
>Mature_534_residues
TPIKRLCMGAAALSILAASAGAAAAESPRPLSDRDARAYAAAFAAADQGDFVGAQIAAAGIKDNSLLGYLSFRQLMHPTA
HKASFEELSGWLSKFRDLPLADRVFSLAAKRKPSGAEAPPAPDVGPDPNAESARKAREAYYSGEPQKAFLLAAAAGERWI
AGLSAYRLKNFPEAEVHFADLARDEDEDSWLRSAAAFWAARSAEALGDAVTAQTFLRLAAQAPETFYGMIAERRVQLVQA
QAPTGQIVLASYRAPPPAPKADPAHLLASQPRAHRAAALVQIGRFEEARQEIRAGLAAAAPDERRAWNRLAADLRLGRAA
AEKPTPGYRILGSDYPVPALEPKWGFTVDRALVYALVWQESRFNPQAVSPAGAIGLMQLMPETAARAAGDDKLKKDASPL
FDPAFNLRVGQDYLTWLMERGVGYDLLRTVAAYNGGPGAVMKAAANAGDDDPLLLIESLPAQETRAYVEKVLAAYWTYKR
MFGEEARSLDALASGARTIDARLDLPDARGPQPELTAKTIQVGLLKADYAGALD

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=156, Percent_Identity=36.5384615384615, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87082191, Length=159, Percent_Identity=33.9622641509434, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57317; Mature: 57186

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIKRLCMGAAALSILAASAGAAAAESPRPLSDRDARAYAAAFAAADQGDFVGAQIAAA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHC
GIKDNSLLGYLSFRQLMHPTAHKASFEELSGWLSKFRDLPLADRVFSLAAKRKPSGAEAP
CCCCCCHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
PAPDVGPDPNAESARKAREAYYSGEPQKAFLLAAAAGERWIAGLSAYRLKNFPEAEVHFA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHH
DLARDEDEDSWLRSAAAFWAARSAEALGDAVTAQTFLRLAAQAPETFYGMIAERRVQLVQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AQAPTGQIVLASYRAPPPAPKADPAHLLASQPRAHRAAALVQIGRFEEARQEIRAGLAAA
HCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APDERRAWNRLAADLRLGRAAAEKPTPGYRILGSDYPVPALEPKWGFTVDRALVYALVWQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESRFNPQAVSPAGAIGLMQLMPETAARAAGDDKLKKDASPLFDPAFNLRVGQDYLTWLME
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEECHHHHHHHHH
RGVGYDLLRTVAAYNGGPGAVMKAAANAGDDDPLLLIESLPAQETRAYVEKVLAAYWTYK
CCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
RMFGEEARSLDALASGARTIDARLDLPDARGPQPELTAKTIQVGLLKADYAGALD
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEECCCCCCC
>Mature Secondary Structure 
TPIKRLCMGAAALSILAASAGAAAAESPRPLSDRDARAYAAAFAAADQGDFVGAQIAAA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHC
GIKDNSLLGYLSFRQLMHPTAHKASFEELSGWLSKFRDLPLADRVFSLAAKRKPSGAEAP
CCCCCCHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
PAPDVGPDPNAESARKAREAYYSGEPQKAFLLAAAAGERWIAGLSAYRLKNFPEAEVHFA
CCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHH
DLARDEDEDSWLRSAAAFWAARSAEALGDAVTAQTFLRLAAQAPETFYGMIAERRVQLVQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AQAPTGQIVLASYRAPPPAPKADPAHLLASQPRAHRAAALVQIGRFEEARQEIRAGLAAA
HCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APDERRAWNRLAADLRLGRAAAEKPTPGYRILGSDYPVPALEPKWGFTVDRALVYALVWQ
CCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ESRFNPQAVSPAGAIGLMQLMPETAARAAGDDKLKKDASPLFDPAFNLRVGQDYLTWLME
HCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEECHHHHHHHHH
RGVGYDLLRTVAAYNGGPGAVMKAAANAGDDDPLLLIESLPAQETRAYVEKVLAAYWTYK
CCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
RMFGEEARSLDALASGARTIDARLDLPDARGPQPELTAKTIQVGLLKADYAGALD
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]