Click here to switch to the map view.

The map label for this gene is gatB [H]

Identifier: 197104792

GI number: 197104792

Start: 1504290

End: 1505789

Strand: Direct

Name: gatB [H]

Synonym: PHZ_c1326

Alternate gene names: 197104792

Gene position: 1504290-1505789 (Clockwise)

Preceding gene: 197104790

Following gene: 197104794

Centisome position: 37.64

GC content: 67.2

Gene sequence:

>1500_bases
ATGAGCGAAACTGCGAACGCCAAGCTGATCCAGGGCCGGACGGGCCCGTGGGAAGTGGTCCTGGGCCTCGAGGTCCACGC
CCAGGTGGCCTCCAACGCCAAGCTGTTCTCCGGCGCGGCCGTCGGATTCGGCGCGGGCCCCAACGAGCAGGTCAGCCTGG
TGGACGCGGCCATGCCCGGCATGCTGCCGGTCATCAACCGCCACTGCGTCGAGCAGGCGGTGCGGACAGGGCTGGGCCTG
AAGGCCCAGATCAACCTCAAGAGCCGCTTCGACCGGAAGAACTACTTCTATCCGGACCTGCCGCAGGGCTATCAGATCAG
CCAGTACCAGCACCCGATCGTGGGTGAGGGCGAGGTCATCGTCGAACGCGACGACGGCTCCACCTTCACGGTCCGGATCG
AGCGGCTGCACCTGGAGCAGGACGCCGGCAAGAGCCTGCACGACCAGGATCCGAACTTCACCTACGTCGACCTGAACCGG
GCCGGCACGGCCCTGATGGAGATCGTCTCCCGGCCCGACATGCGCTCGTCCGACGAGGCGGCGGCCTATGTGAAGAAGCT
GCGGACCATCCTGATCTACCTCGGCTCCTGCGACGGCGACATGGAGAAGGGCAACCTGCGGGCGGACGTGAACGTCTCGG
TCTGCCGCCCCGGCGCCTACGAGAAGTACCGCCAGACCGGCGACTTCAGCCATCTGGGCACGCGCTGCGAGATCAAGAAC
GTCAACTCGTTCCGCTTCATGCAGCAGGCGATCGAGTACGAGGCCCGCCGCCAGATCGAGATTCTGGAGGACGGCGGTTC
CATCGACCAGGAGACCCGCCTGTTCGATCCGAACAAGGGCGAGACGCGGTCCATGCGCTCGAAGGAAGAGGCGCACGACT
ACCGCTACTTCCCCGACCCCGACCTGCTGCCGCTGGAGCTCGACCCGGCCTGGGTGAAGAAGATCGAGGCGGACCTGCCG
GAACTGCCCGACGCCAAGAAGGCGCGCTTCCAGTCGCAGTACGGCCTGTCGGCCTACGACGCCGGCGTGCTGATCGCCGA
CCAGGCCAAGGCCGACTTCTACGAGGCCGCGGCCAACGGCCGCGACGCCAAGCTCGTGGCCAACTGGGTCACCAACGACC
TGGCCGCCAAGCTGTCGGCGGACGGACTGGAGATCGGCGACTCGCCGATCGGCCCGGCGGCGATCGCCGAGCTGGTCCAG
CTGATCGAGGACGGGACCATCTCGTCCAAGATCGCCAAGGACGTGTTCGAGCGGATGTGGGCGGGCGAGGGCTCGGCGTC
CGAGATCGTCGAGAAGCAGGGCCTGAAGCAGGTTTCCGACACCGGCGCGCTGGAGAAGATCATCGACGACCTCATCGCCG
CCAATCCCGGCCAGGCCGCCGCGGTCAAGGAGAAGCCGCAGGCCATCGGCTGGTTCGTGGGCCAGGTGATGAAGGCGACG
GGCGGCAAGGCCAACCCCGGCGCCGTCAACCAGCTGCTGAAGGCGAAACTGGGCGTCTAG

Upstream 100 bases:

>100_bases
CGTAAGGCGGAGCGCAGCTTGAGGCCATTTCATGACCTTTGCGCAAGAGGGCGCGTGAGCGCGCTTCACCTTCCGGCCCG
GCGGCGCTAGGGAAGCGGCC

Downstream 100 bases:

>100_bases
TCCGGGACGATGTCGAAGACCCGGCCGTTCGGGTCGACCAGCACGAAGCCGTCCCCCATCCGCCGCCAGGCGTAGCCGCG
CGGCGGCTGGCGCAGGCGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSETANAKLIQGRTGPWEVVLGLEVHAQVASNAKLFSGAAVGFGAGPNEQVSLVDAAMPGMLPVINRHCVEQAVRTGLGL
KAQINLKSRFDRKNYFYPDLPQGYQISQYQHPIVGEGEVIVERDDGSTFTVRIERLHLEQDAGKSLHDQDPNFTYVDLNR
AGTALMEIVSRPDMRSSDEAAAYVKKLRTILIYLGSCDGDMEKGNLRADVNVSVCRPGAYEKYRQTGDFSHLGTRCEIKN
VNSFRFMQQAIEYEARRQIEILEDGGSIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLELDPAWVKKIEADLP
ELPDAKKARFQSQYGLSAYDAGVLIADQAKADFYEAAANGRDAKLVANWVTNDLAAKLSADGLEIGDSPIGPAAIAELVQ
LIEDGTISSKIAKDVFERMWAGEGSASEIVEKQGLKQVSDTGALEKIIDDLIAANPGQAAAVKEKPQAIGWFVGQVMKAT
GGKANPGAVNQLLKAKLGV

Sequences:

>Translated_499_residues
MSETANAKLIQGRTGPWEVVLGLEVHAQVASNAKLFSGAAVGFGAGPNEQVSLVDAAMPGMLPVINRHCVEQAVRTGLGL
KAQINLKSRFDRKNYFYPDLPQGYQISQYQHPIVGEGEVIVERDDGSTFTVRIERLHLEQDAGKSLHDQDPNFTYVDLNR
AGTALMEIVSRPDMRSSDEAAAYVKKLRTILIYLGSCDGDMEKGNLRADVNVSVCRPGAYEKYRQTGDFSHLGTRCEIKN
VNSFRFMQQAIEYEARRQIEILEDGGSIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLELDPAWVKKIEADLP
ELPDAKKARFQSQYGLSAYDAGVLIADQAKADFYEAAANGRDAKLVANWVTNDLAAKLSADGLEIGDSPIGPAAIAELVQ
LIEDGTISSKIAKDVFERMWAGEGSASEIVEKQGLKQVSDTGALEKIIDDLIAANPGQAAAVKEKPQAIGWFVGQVMKAT
GGKANPGAVNQLLKAKLGV
>Mature_498_residues
SETANAKLIQGRTGPWEVVLGLEVHAQVASNAKLFSGAAVGFGAGPNEQVSLVDAAMPGMLPVINRHCVEQAVRTGLGLK
AQINLKSRFDRKNYFYPDLPQGYQISQYQHPIVGEGEVIVERDDGSTFTVRIERLHLEQDAGKSLHDQDPNFTYVDLNRA
GTALMEIVSRPDMRSSDEAAAYVKKLRTILIYLGSCDGDMEKGNLRADVNVSVCRPGAYEKYRQTGDFSHLGTRCEIKNV
NSFRFMQQAIEYEARRQIEILEDGGSIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDPDLLPLELDPAWVKKIEADLPE
LPDAKKARFQSQYGLSAYDAGVLIADQAKADFYEAAANGRDAKLVANWVTNDLAAKLSADGLEIGDSPIGPAAIAELVQL
IEDGTISSKIAKDVFERMWAGEGSASEIVEKQGLKQVSDTGALEKIIDDLIAANPGQAAAVKEKPQAIGWFVGQVMKATG
GKANPGAVNQLLKAKLGV

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=506, Percent_Identity=43.2806324110672, Blast_Score=380, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI115534094, Length=309, Percent_Identity=41.747572815534, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319391, Length=548, Percent_Identity=28.6496350364964, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45550808, Length=503, Percent_Identity=36.779324055666, Blast_Score=308, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54516; Mature: 54385

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETANAKLIQGRTGPWEVVLGLEVHAQVASNAKLFSGAAVGFGAGPNEQVSLVDAAMPG
CCCCCCCEEEECCCCCEEEEEEEEEEHHHHCCCEEECCCEEECCCCCCCCEEEHHHHCCC
MLPVINRHCVEQAVRTGLGLKAQINLKSRFDRKNYFYPDLPQGYQISQYQHPIVGEGEVI
HHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
VERDDGSTFTVRIERLHLEQDAGKSLHDQDPNFTYVDLNRAGTALMEIVSRPDMRSSDEA
EECCCCCEEEEEEEEEEEHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHH
AAYVKKLRTILIYLGSCDGDMEKGNLRADVNVSVCRPGAYEKYRQTGDFSHLGTRCEIKN
HHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHCCCEEEECC
VNSFRFMQQAIEYEARRQIEILEDGGSIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDP
CHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
DLLPLELDPAWVKKIEADLPELPDAKKARFQSQYGLSAYDAGVLIADQAKADFYEAAANG
CEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHCCC
RDAKLVANWVTNDLAAKLSADGLEIGDSPIGPAAIAELVQLIEDGTISSKIAKDVFERMW
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AGEGSASEIVEKQGLKQVSDTGALEKIIDDLIAANPGQAAAVKEKPQAIGWFVGQVMKAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHC
GGKANPGAVNQLLKAKLGV
CCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SETANAKLIQGRTGPWEVVLGLEVHAQVASNAKLFSGAAVGFGAGPNEQVSLVDAAMPG
CCCCCCEEEECCCCCEEEEEEEEEEHHHHCCCEEECCCEEECCCCCCCCEEEHHHHCCC
MLPVINRHCVEQAVRTGLGLKAQINLKSRFDRKNYFYPDLPQGYQISQYQHPIVGEGEVI
HHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
VERDDGSTFTVRIERLHLEQDAGKSLHDQDPNFTYVDLNRAGTALMEIVSRPDMRSSDEA
EECCCCCEEEEEEEEEEEHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCHHH
AAYVKKLRTILIYLGSCDGDMEKGNLRADVNVSVCRPGAYEKYRQTGDFSHLGTRCEIKN
HHHHHHHHHHHEEEECCCCCCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHCCCEEEECC
VNSFRFMQQAIEYEARRQIEILEDGGSIDQETRLFDPNKGETRSMRSKEEAHDYRYFPDP
CHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
DLLPLELDPAWVKKIEADLPELPDAKKARFQSQYGLSAYDAGVLIADQAKADFYEAAANG
CEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHCCC
RDAKLVANWVTNDLAAKLSADGLEIGDSPIGPAAIAELVQLIEDGTISSKIAKDVFERMW
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AGEGSASEIVEKQGLKQVSDTGALEKIIDDLIAANPGQAAAVKEKPQAIGWFVGQVMKAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHC
GGKANPGAVNQLLKAKLGV
CCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]