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The map label for this gene is gatA

Identifier: 197104790

GI number: 197104790

Start: 1502212

End: 1503684

Strand: Direct

Name: gatA

Synonym: PHZ_c1324

Alternate gene names: 197104790

Gene position: 1502212-1503684 (Clockwise)

Preceding gene: 197104789

Following gene: 197104792

Centisome position: 37.59

GC content: 70.74

Gene sequence:

>1473_bases
ATGGCCGAGCTCACCTCCCTGACGCTGAAGGCCGCGCTGGACGGCCTGTCGTCGAAGTCGTTCTCCTCGGTGGAGCTGAC
CCAGGCCCACCTCGAGGCCGTGGAGGCCGCGCGGGCGCTGAACGCCTATGTGGTCGAAACGCCCGACCGGGCGCTCGAGA
TGGCCAGGGCCGCCGACGCGCGCCGCGCCCGGGGCGAGGCCGGCCGCCTGGAAGGCGCGCCGCTGGGCATCAAGGACCTG
TTCTGCACGGAAGGCGTGGAGTCCACCGCCGCCTCCAAGATCCTGAAGGGCTTCAAGCCGACCTACGAATCCACCGTGAC
CGCCAACCTCTGGCGCGACGGGGCGGTCATGCTGGGCAAGCTCAACATGGACGAGTTCGCCATGGGCTCGTCCAACGAGA
CCTCGGCCTGGGGCCCGGTGGTCAACCCGTGGCGGGCCGAGGGCGGCAACGCCCAGCTGACCCCGGGCGGCTCGTCCGGC
GGCTCGGCCTCGGCGGTCGCCGCCGAGCTCTGCCTGGGCGCCACGGCCACCGACACCGGCGGCTCGATCCGCCAGCCCGC
CGCCTTCACCGGCACGGTGGGCATCAAGCCGACCTACGGCCGCTGCTCGCGCTGGGGCATCGTCGCCTTCGCGTCCTCGC
TGGACCAGGCCGGGCCGATGGCGCGCACCGTCGAGGACGCGGCCATCCTGCTGACCTCGATGTCGGGGCACGACCCCAAG
GACTCCACCAGCCTGCCCGTCGAGACGCCGGACTTCGCCAGCTTCTGCGGCAAGTCGGTGAAGGGCCTGCGGGTCGGCAT
CCCGAAGGAATACCGCGTCGACGGCATGCCGCCCGAGATCGAGAAGCTGTGGGAGCAGGGGATCGCCTGGCTGAAGGAGG
CCGGCTGCGAAATCGTCGAGGTCAGCCTGCCGCACACCAAGTACGCCCTGCCGGCCTACTACATCATCGCGCCCGCCGAG
GCCTCGTCGAACCTCGCCCGCTACGACGGCATGCGCTTCGGCCTGCGCGTCGAGGGCGGCAACCTCACCGAGACGTACGA
GAACACCCGCGCCGAGGGCTTCGGGGCCGAGGTGAAGCGGCGGATCCTGATCGGCACCTACGTGCTCTCCGCCGGCTACT
ACGACGCCTACTACCTCAAGGCCCTGAAGGTCCGCCGGCGCATCGCCGACGACTTCGACCGGGCCTGGGAGAAGGTGGAC
GCGCTGCTGACGCCGACCGCGCCGTCGGCCGCCTTCGCCCTGGGCGAGAAGTCGGACGATCCGGTGGCCATGTACCTGAA
CGACATCTTCACGGTGACGGTGAACCTGGCGGGCCTGCCGGGCATGAGCGTTCCGGCCGGCGTCGACGCCAACGGCCTGC
CGCTGGGCCTGCAGCTGATCGGCAAGGCGCTGGACGAGGGCACGCTGTTCTCGCTGGGCTCGGCCATCGAGAAGGCGGCG
GACTTCAAGGCCAAGCCGAAGAAGTGGTGGTGA

Upstream 100 bases:

>100_bases
GGGACGACGTCGTCACCGAGGGCGGCGATCCCGCCAAGGTGCTGGCCAACGCGCCGAAGGCCGCCAAGAACTTCTTCGTC
GTGCCGAAGGTGGTCGAGTA

Downstream 100 bases:

>100_bases
TCCTTTCCCTCCCTTGAGTGGGGGAGGGTGGAGTCGCGCCCGGCGCGAGCCGGGTGGGGAGGTCATGATCCGGCGCTGCG
CCCGATCCCTGGCTGCCCCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAELTSLTLKAALDGLSSKSFSSVELTQAHLEAVEAARALNAYVVETPDRALEMARAADARRARGEAGRLEGAPLGIKDL
FCTEGVESTAASKILKGFKPTYESTVTANLWRDGAVMLGKLNMDEFAMGSSNETSAWGPVVNPWRAEGGNAQLTPGGSSG
GSASAVAAELCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMARTVEDAAILLTSMSGHDPK
DSTSLPVETPDFASFCGKSVKGLRVGIPKEYRVDGMPPEIEKLWEQGIAWLKEAGCEIVEVSLPHTKYALPAYYIIAPAE
ASSNLARYDGMRFGLRVEGGNLTETYENTRAEGFGAEVKRRILIGTYVLSAGYYDAYYLKALKVRRRIADDFDRAWEKVD
ALLTPTAPSAAFALGEKSDDPVAMYLNDIFTVTVNLAGLPGMSVPAGVDANGLPLGLQLIGKALDEGTLFSLGSAIEKAA
DFKAKPKKWW

Sequences:

>Translated_490_residues
MAELTSLTLKAALDGLSSKSFSSVELTQAHLEAVEAARALNAYVVETPDRALEMARAADARRARGEAGRLEGAPLGIKDL
FCTEGVESTAASKILKGFKPTYESTVTANLWRDGAVMLGKLNMDEFAMGSSNETSAWGPVVNPWRAEGGNAQLTPGGSSG
GSASAVAAELCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMARTVEDAAILLTSMSGHDPK
DSTSLPVETPDFASFCGKSVKGLRVGIPKEYRVDGMPPEIEKLWEQGIAWLKEAGCEIVEVSLPHTKYALPAYYIIAPAE
ASSNLARYDGMRFGLRVEGGNLTETYENTRAEGFGAEVKRRILIGTYVLSAGYYDAYYLKALKVRRRIADDFDRAWEKVD
ALLTPTAPSAAFALGEKSDDPVAMYLNDIFTVTVNLAGLPGMSVPAGVDANGLPLGLQLIGKALDEGTLFSLGSAIEKAA
DFKAKPKKWW
>Mature_489_residues
AELTSLTLKAALDGLSSKSFSSVELTQAHLEAVEAARALNAYVVETPDRALEMARAADARRARGEAGRLEGAPLGIKDLF
CTEGVESTAASKILKGFKPTYESTVTANLWRDGAVMLGKLNMDEFAMGSSNETSAWGPVVNPWRAEGGNAQLTPGGSSGG
SASAVAAELCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMARTVEDAAILLTSMSGHDPKD
STSLPVETPDFASFCGKSVKGLRVGIPKEYRVDGMPPEIEKLWEQGIAWLKEAGCEIVEVSLPHTKYALPAYYIIAPAEA
SSNLARYDGMRFGLRVEGGNLTETYENTRAEGFGAEVKRRILIGTYVLSAGYYDAYYLKALKVRRRIADDFDRAWEKVDA
LLTPTAPSAAFALGEKSDDPVAMYLNDIFTVTVNLAGLPGMSVPAGVDANGLPLGLQLIGKALDEGTLFSLGSAIEKAAD
FKAKPKKWW

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=40.6438631790744, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI195972892, Length=485, Percent_Identity=23.5051546391753, Blast_Score=96, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=37.9232505643341, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=25.1485148514851, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=325, Percent_Identity=28.6153846153846, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=298, Percent_Identity=26.1744966442953, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=24.8447204968944, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=331, Percent_Identity=25.9818731117825, Blast_Score=65, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=36.1111111111111, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=463, Percent_Identity=26.7818574514039, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648113, Length=493, Percent_Identity=39.1480730223124, Blast_Score=305, Evalue=4e-83,
Organism=Drosophila melanogaster, GI24652985, Length=496, Percent_Identity=24.5967741935484, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922090, Length=496, Percent_Identity=24.5967741935484, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652981, Length=496, Percent_Identity=24.5967741935484, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24652983, Length=496, Percent_Identity=24.5967741935484, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24644968, Length=485, Percent_Identity=23.9175257731959, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=24.75442043222, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI45550774, Length=244, Percent_Identity=28.2786885245902, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648435, Length=244, Percent_Identity=28.2786885245902, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648437, Length=244, Percent_Identity=28.2786885245902, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356731, Length=219, Percent_Identity=30.5936073059361, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PHEZH (B4R968)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130167.1
- GeneID:   6790828
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1324
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52015; Mature: 51884

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTSLTLKAALDGLSSKSFSSVELTQAHLEAVEAARALNAYVVETPDRALEMARAADA
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHH
RRARGEAGRLEGAPLGIKDLFCTEGVESTAASKILKGFKPTYESTVTANLWRDGAVMLGK
HHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHCEEEEECCCCCCEEEEE
LNMDEFAMGSSNETSAWGPVVNPWRAEGGNAQLTPGGSSGGSASAVAAELCLGATATDTG
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMARTVEDAAILLTSMSGHDPK
CCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCC
DSTSLPVETPDFASFCGKSVKGLRVGIPKEYRVDGMPPEIEKLWEQGIAWLKEAGCEIVE
CCCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
VSLPHTKYALPAYYIIAPAEASSNLARYDGMRFGLRVEGGNLTETYENTRAEGFGAEVKR
EECCCCCCCCCEEEEEEECCCCCCHHHHCCEEEEEEECCCCCCHHHHHHCCCCCCHHHHH
RILIGTYVLSAGYYDAYYLKALKVRRRIADDFDRAWEKVDALLTPTAPSAAFALGEKSDD
HHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCC
PVAMYLNDIFTVTVNLAGLPGMSVPAGVDANGLPLGLQLIGKALDEGTLFSLGSAIEKAA
CCEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
DFKAKPKKWW
CCCCCCCCCC
>Mature Secondary Structure 
AELTSLTLKAALDGLSSKSFSSVELTQAHLEAVEAARALNAYVVETPDRALEMARAADA
CCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHH
RRARGEAGRLEGAPLGIKDLFCTEGVESTAASKILKGFKPTYESTVTANLWRDGAVMLGK
HHHCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHCEEEEECCCCCCEEEEE
LNMDEFAMGSSNETSAWGPVVNPWRAEGGNAQLTPGGSSGGSASAVAAELCLGATATDTG
CCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGIVAFASSLDQAGPMARTVEDAAILLTSMSGHDPK
CCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHEEEEECCCCCCC
DSTSLPVETPDFASFCGKSVKGLRVGIPKEYRVDGMPPEIEKLWEQGIAWLKEAGCEIVE
CCCCCCCCCCHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEE
VSLPHTKYALPAYYIIAPAEASSNLARYDGMRFGLRVEGGNLTETYENTRAEGFGAEVKR
EECCCCCCCCCEEEEEEECCCCCCHHHHCCEEEEEEECCCCCCHHHHHHCCCCCCHHHHH
RILIGTYVLSAGYYDAYYLKALKVRRRIADDFDRAWEKVDALLTPTAPSAAFALGEKSDD
HHHEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEECCCCCCC
PVAMYLNDIFTVTVNLAGLPGMSVPAGVDANGLPLGLQLIGKALDEGTLFSLGSAIEKAA
CCEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
DFKAKPKKWW
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA