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The map label for this gene is hdhA [C]

Identifier: 197104779

GI number: 197104779

Start: 1489804

End: 1490664

Strand: Reverse

Name: hdhA [C]

Synonym: PHZ_c1313

Alternate gene names: 197104779

Gene position: 1490664-1489804 (Counterclockwise)

Preceding gene: 197104780

Following gene: 197104778

Centisome position: 37.3

GC content: 72.59

Gene sequence:

>861_bases
ATGGCTTCCGAAGGCCCCATCGAAGGAATTCCCGCCCTCGCCCCCGGCGGTGCGGCCGTCGTGACCGGCGCGGCCAGCGG
CATCGGCCTCGAAGCCGCCTCCACCTTCGCCCGCCGGGGGCTGAAGGTGGTGATGGCCGACCTGGGCGGCGAGGCCCTGG
ACCAGTCCGCCGACCTCGTCCGCGCCGCCGCCGGCTCGGCCGAGGTGCTGGCCGTGCCCACCGACGTCGCCCGATTCGAG
GAGATGGAGCGCCTGCGCGACCGCGCGCTGGAGGCGTTCGGCGTCCCCGCCGTCCTGATGAACAACGCCGGCGTCGGCCT
CAACCCGGGCGGCGTGGCGAAGGACCTGGCGGCCTGGCGGCGGCTGATGGACGTGAACTTCTGGGGCGTCGCCCACGGCG
TCCAGGCGTTCCTGCCGGCCATGACCGAGGCGGGCGGGCCGCGGCTCATCATCAACACCGGCTCGAAGCAGGGCATCACC
ACCCCGCCGGGCAACGCCGCCTACAACGTCTCCAAGGCCGCGGTGAAGGTGTTCACCGAGGCCCTCGCGCACGAGCTGCG
CCAGGACCCCGCCTGCCGGATCACGGCCCACCTGCTGATCCCCGGCTTCACGCACACCGGCATGACCTCCCGCGGCGAGA
AGCACCCCGCCGCCTGGACGGCGGCCCAGGTGGTGGAGTTCATGCTGCAGGGCCTCGCGCGCGGCGACTTCTACATCCTG
TGTCCCGACAACGACGTGGACCGCGCCACCGACGTGCGACGCATGCAGTGGGCGATCGGCGACATCGCCGAGAACCGCCC
GGCCCTGTCGCGCTGGCATCCCGACTGGAAGGACGCCTTCGACGCCTTCATGAAGGGCTGA

Upstream 100 bases:

>100_bases
GCCCAGGCGCGGATGGAGGACCCGTCGCGCCCGAAGCCGTTCCTGAGGTACAAGGACCGGTCCGCCGGACGTCCCGCCTA
CCTCTAGCCGCGAGGCTCGC

Downstream 100 bases:

>100_bases
CCAGGAAAGGAGACGCCGCATGGCCCGCCTGCTCGCCGCGATCGCCACGACCGCCGCCCTCGTCCTCCACGCTTCCGCAG
CGGCCGCCGCGGAGCGCCCG

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MASEGPIEGIPALAPGGAAVVTGAASGIGLEAASTFARRGLKVVMADLGGEALDQSADLVRAAAGSAEVLAVPTDVARFE
EMERLRDRALEAFGVPAVLMNNAGVGLNPGGVAKDLAAWRRLMDVNFWGVAHGVQAFLPAMTEAGGPRLIINTGSKQGIT
TPPGNAAYNVSKAAVKVFTEALAHELRQDPACRITAHLLIPGFTHTGMTSRGEKHPAAWTAAQVVEFMLQGLARGDFYIL
CPDNDVDRATDVRRMQWAIGDIAENRPALSRWHPDWKDAFDAFMKG

Sequences:

>Translated_286_residues
MASEGPIEGIPALAPGGAAVVTGAASGIGLEAASTFARRGLKVVMADLGGEALDQSADLVRAAAGSAEVLAVPTDVARFE
EMERLRDRALEAFGVPAVLMNNAGVGLNPGGVAKDLAAWRRLMDVNFWGVAHGVQAFLPAMTEAGGPRLIINTGSKQGIT
TPPGNAAYNVSKAAVKVFTEALAHELRQDPACRITAHLLIPGFTHTGMTSRGEKHPAAWTAAQVVEFMLQGLARGDFYIL
CPDNDVDRATDVRRMQWAIGDIAENRPALSRWHPDWKDAFDAFMKG
>Mature_285_residues
ASEGPIEGIPALAPGGAAVVTGAASGIGLEAASTFARRGLKVVMADLGGEALDQSADLVRAAAGSAEVLAVPTDVARFEE
MERLRDRALEAFGVPAVLMNNAGVGLNPGGVAKDLAAWRRLMDVNFWGVAHGVQAFLPAMTEAGGPRLIINTGSKQGITT
PPGNAAYNVSKAAVKVFTEALAHELRQDPACRITAHLLIPGFTHTGMTSRGEKHPAAWTAAQVVEFMLQGLARGDFYILC
PDNDVDRATDVRRMQWAIGDIAENRPALSRWHPDWKDAFDAFMKG

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=201, Percent_Identity=28.8557213930348, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI40807363, Length=193, Percent_Identity=30.0518134715026, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25282469, Length=220, Percent_Identity=29.5454545454545, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI31542939, Length=216, Percent_Identity=28.2407407407407, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787905, Length=131, Percent_Identity=34.3511450381679, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082100, Length=195, Percent_Identity=31.2820512820513, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=215, Percent_Identity=33.0232558139535, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786701, Length=193, Percent_Identity=30.0518134715026, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147288, Length=202, Percent_Identity=33.6633663366337, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562908, Length=180, Percent_Identity=31.1111111111111, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17561402, Length=196, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=198, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=205, Percent_Identity=30.2439024390244, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=179, Percent_Identity=28.4916201117318, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=199, Percent_Identity=28.643216080402, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=191, Percent_Identity=30.3664921465969, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=193, Percent_Identity=26.9430051813472, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=198, Percent_Identity=30.8080808080808, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=190, Percent_Identity=25.2631578947368, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=199, Percent_Identity=27.6381909547739, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=198, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=171, Percent_Identity=32.7485380116959, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644339, Length=198, Percent_Identity=31.3131313131313, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571526, Length=194, Percent_Identity=35.5670103092784, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364892, Length=206, Percent_Identity=31.5533980582524, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21358495, Length=197, Percent_Identity=27.9187817258883, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357041, Length=201, Percent_Identity=31.3432835820896, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI18859837, Length=189, Percent_Identity=32.2751322751323, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24649181, Length=246, Percent_Identity=25.2032520325203, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI161076933, Length=225, Percent_Identity=29.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584748, Length=225, Percent_Identity=29.3333333333333, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 30208; Mature: 30077

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASEGPIEGIPALAPGGAAVVTGAASGIGLEAASTFARRGLKVVMADLGGEALDQSADLV
CCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCHHEEHHHHCCHHHHHHHHHH
RAAAGSAEVLAVPTDVARFEEMERLRDRALEAFGVPAVLMNNAGVGLNPGGVAKDLAAWR
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHH
RLMDVNFWGVAHGVQAFLPAMTEAGGPRLIINTGSKQGITTPPGNAAYNVSKAAVKVFTE
HHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALAHELRQDPACRITAHLLIPGFTHTGMTSRGEKHPAAWTAAQVVEFMLQGLARGDFYIL
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
CPDNDVDRATDVRRMQWAIGDIAENRPALSRWHPDWKDAFDAFMKG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
ASEGPIEGIPALAPGGAAVVTGAASGIGLEAASTFARRGLKVVMADLGGEALDQSADLV
CCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCHHEEHHHHCCHHHHHHHHHH
RAAAGSAEVLAVPTDVARFEEMERLRDRALEAFGVPAVLMNNAGVGLNPGGVAKDLAAWR
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHH
RLMDVNFWGVAHGVQAFLPAMTEAGGPRLIINTGSKQGITTPPGNAAYNVSKAAVKVFTE
HHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALAHELRQDPACRITAHLLIPGFTHTGMTSRGEKHPAAWTAAQVVEFMLQGLARGDFYIL
HHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
CPDNDVDRATDVRRMQWAIGDIAENRPALSRWHPDWKDAFDAFMKG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA