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The map label for this gene is mak [H]

Identifier: 197104771

GI number: 197104771

Start: 1484272

End: 1485132

Strand: Reverse

Name: mak [H]

Synonym: PHZ_c1305

Alternate gene names: 197104771

Gene position: 1485132-1484272 (Counterclockwise)

Preceding gene: 197104773

Following gene: 197104770

Centisome position: 37.16

GC content: 77.12

Gene sequence:

>861_bases
ATGATCCGCTTCGGCGTGGACTTCGGCGGAACCAAGATCGAGGCCGCGGCGCTGGACGCTGACGGCCGCTTCGTCGCGCG
GGTGCGGGCGCCGACCCCGAAAGCCTATGACGAGGGGCTGGAGGCCGTGGCCGAACTCCTGGCCGAGGCCGAGCGGCAGG
CCGGGGCCAGGGCCGAACGCGTCGGCGTGGGCGGGCCGGGCTCGCCCTCACCGGCCGGCGGACTGATGCGCAACGCCAAC
TCCACCCAGCTCAACGGCCGCCCCTTCCCGGCCGACCTGGCACGGATGCTGGGGCGGCCGGTGCGCTACGAGAACGACGC
CAACTGCCTGGCGCTCTCGGAGGCGACCGACGGCGCCGGCGCGGGCGCCGAGCTGGTGTTCGCCGTCATCCTGGGAACCG
GCTGCGGCGGCGGGATCGCCATCGGCGGGCGGCCGCGCACCGGCCGCAACGCCGTGGCCGGCGAGTGGGGCCACACGCCC
CTGCCCTGGCCGCGCCCCGACGAGCTGCCGGCGCCGGAATGCTGGTGCGGCCGCCGCGGATGCCTGGAGCTGTGGGTCAG
CGGTCCCGCCTTCGCCCGCGACGCGGGCCGCCCCGCCGAGGCGGCCGCGGCCGCCGGGGGCCCGCCGCTGGACCGGTACA
TCGACCGGCTGGGGCGCGGGCTCGCGGCCGTCGTGGACGTGCTGGACCCGGACGTGATCGTCCTGGGCGGCGGCATGTCG
AACGTGGAGGCGCTTTACGACCGGCTGCCGGCCGCCATCGCCCCGCACGTGTTCTCGGACGTGTTCGCGACGCCGGTGCG
CCGGGCCGCGCACGGGGACTCCTCGGGCGTGCGCGGCGCTGCATGGCTGTGGCCGCGATGA

Upstream 100 bases:

>100_bases
ATCGCCTCCCGCCGCCGCCCATAACGCCAAGGCGGCGCTTTCTTTCCGCCCCCGCCATCAGGCGCGTTATCGGCCGGAAC
AAATCAGGTATCCTGGCGCC

Downstream 100 bases:

>100_bases
AGGCCCTCTGCCGCGATTGCCTCTGGACGGGGGAGGACCAGCCGCGCCGCTGTCCGGCCTGCGGCTCGCCGCGGATCGTG
GCCCACGAGGAGCTGGGCGC

Product: putative transcriptional regulator/sugar kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MIRFGVDFGGTKIEAAALDADGRFVARVRAPTPKAYDEGLEAVAELLAEAERQAGARAERVGVGGPGSPSPAGGLMRNAN
STQLNGRPFPADLARMLGRPVRYENDANCLALSEATDGAGAGAELVFAVILGTGCGGGIAIGGRPRTGRNAVAGEWGHTP
LPWPRPDELPAPECWCGRRGCLELWVSGPAFARDAGRPAEAAAAAGGPPLDRYIDRLGRGLAAVVDVLDPDVIVLGGGMS
NVEALYDRLPAAIAPHVFSDVFATPVRRAAHGDSSGVRGAAWLWPR

Sequences:

>Translated_286_residues
MIRFGVDFGGTKIEAAALDADGRFVARVRAPTPKAYDEGLEAVAELLAEAERQAGARAERVGVGGPGSPSPAGGLMRNAN
STQLNGRPFPADLARMLGRPVRYENDANCLALSEATDGAGAGAELVFAVILGTGCGGGIAIGGRPRTGRNAVAGEWGHTP
LPWPRPDELPAPECWCGRRGCLELWVSGPAFARDAGRPAEAAAAAGGPPLDRYIDRLGRGLAAVVDVLDPDVIVLGGGMS
NVEALYDRLPAAIAPHVFSDVFATPVRRAAHGDSSGVRGAAWLWPR
>Mature_286_residues
MIRFGVDFGGTKIEAAALDADGRFVARVRAPTPKAYDEGLEAVAELLAEAERQAGARAERVGVGGPGSPSPAGGLMRNAN
STQLNGRPFPADLARMLGRPVRYENDANCLALSEATDGAGAGAELVFAVILGTGCGGGIAIGGRPRTGRNAVAGEWGHTP
LPWPRPDELPAPECWCGRRGCLELWVSGPAFARDAGRPAEAAAAAGGPPLDRYIDRLGRGLAAVVDVLDPDVIVLGGGMS
NVEALYDRLPAAIAPHVFSDVFATPVRRAAHGDSSGVRGAAWLWPR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=272, Percent_Identity=29.0441176470588, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI190014632, Length=272, Percent_Identity=29.0441176470588, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI4885285, Length=272, Percent_Identity=29.0441176470588, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI298566315, Length=272, Percent_Identity=29.0441176470588, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI87081733, Length=302, Percent_Identity=51.9867549668874, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1787363, Length=300, Percent_Identity=37.6666666666667, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1790522, Length=293, Percent_Identity=28.3276450511945, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 29573; Mature: 29573

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFGVDFGGTKIEAAALDADGRFVARVRAPTPKAYDEGLEAVAELLAEAERQAGARAER
CEEECCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
VGVGGPGSPSPAGGLMRNANSTQLNGRPFPADLARMLGRPVRYENDANCLALSEATDGAG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
AGAELVFAVILGTGCGGGIAIGGRPRTGRNAVAGEWGHTPLPWPRPDELPAPECWCGRRG
CHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
CLELWVSGPAFARDAGRPAEAAAAAGGPPLDRYIDRLGRGLAAVVDVLDPDVIVLGGGMS
CEEEEECCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEECCCCH
NVEALYDRLPAAIAPHVFSDVFATPVRRAAHGDSSGVRGAAWLWPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRFGVDFGGTKIEAAALDADGRFVARVRAPTPKAYDEGLEAVAELLAEAERQAGARAER
CEEECCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCHHHH
VGVGGPGSPSPAGGLMRNANSTQLNGRPFPADLARMLGRPVRYENDANCLALSEATDGAG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCC
AGAELVFAVILGTGCGGGIAIGGRPRTGRNAVAGEWGHTPLPWPRPDELPAPECWCGRRG
CHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCC
CLELWVSGPAFARDAGRPAEAAAAAGGPPLDRYIDRLGRGLAAVVDVLDPDVIVLGGGMS
CEEEEECCCHHHHCCCCCHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCCEEEECCCCH
NVEALYDRLPAAIAPHVFSDVFATPVRRAAHGDSSGVRGAAWLWPR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]