Click here to switch to the map view.

The map label for this gene is dacA [H]

Identifier: 197104762

GI number: 197104762

Start: 1475247

End: 1476500

Strand: Reverse

Name: dacA [H]

Synonym: PHZ_c1296

Alternate gene names: 197104762

Gene position: 1476500-1475247 (Counterclockwise)

Preceding gene: 197104768

Following gene: 197104759

Centisome position: 36.95

GC content: 69.22

Gene sequence:

>1254_bases
ATGTTCCAGCACGCCCGCCGCCTTGTGCTTTCCCTGGGTCTTGCCGCCGCCGCGGCGTTCGGCGCGATGGCGCCCCCTGC
GCAGGCGCAGATCCCCTATCAGATGCTCGCGCAGACCTCGTCGAAGTACGCGGCCATCGTCGTGGACGCGAAGACGGGCG
AGGTTCTGTACGAGAACCGGGCCGACAGCCCCCGGTACCCGGCGTCGATCACCAAGGTCATGACGCTGTACCTGACCTTC
GAGGCGCTGGCCTCGGGCAAGATCGACCTGGACGACCGGGTCGTCTTCTCGCCGCGCGCGGCGGCCCAGCCGCCGACGAA
GCTGGGCGTGCGGGCCGGCGACTCCATCTCGGTGGACGAGGCCATGAAGGCCCTTGCCACCAAGTCGGCCAACGACGCGG
CCGTCGCCCTCGCCGAGAAGCTGGCGGGCACCGAGCAGCGCTTCGCTGCGCTGATGACCCTGCGGGCGCGCGAGCTCGGC
ATGACCGACACCAACTTCGCCAACGCCTCGGGCCTGCCCGACAGCCGCCAGGTCTCGACGGCGCGTGACATCGCGATCCT
GTCGCGGGCGGTGATGCGGGACTACCCGCAGTACTACAAGCTGTTCAGCCTCCAGTATTTCAACTTCCGCGGCCAGACGA
TGCGCAACCACAATGGCCTGCTGCACCGGATGGACGGCGTCGACGGGCTGAAGACCGGCTTCATCAACGCCTCGGGCTTC
AACCTGGCCGCGTCGGCCGTGCGCGACGGCCGCCGCCTCATCGCGGTGGTCCTGGGCGGCCCCACCAGCCGCGCCCGGGA
CAACAACGCCGAGAGCCTGCTGCTGACCGGCTTCGAGGTCCTGGACCGCCGCGCTCGCGGCGAGGACATCCAGGTGGCCC
AGAGCTTCTTCGAGTCCCCGATGACGGGCGGCCGCGTTCAGCCGCAGACCGCCCAGGGCGACGAGGACAGCATGAAGATC
GTGCTCGCCTCGACCGCTCCGCCGATCCGCGCCTCGAAGGTCGAGATCGTCGAGAAGGGCAAGTGGACGGTCCAGGTCGG
GTCGTTCTCGAGCCGGTCCGACGCCAAGGAGCAGCTCTCCATCGTCGAGAAGCGCTTCGGCAAGCACTTCGGCAAGGCCA
AGGGCGTCGCCGAGAAGTCCGGCGGCAAGTACCGCTCGCGCTTCTCCGGCTTCTCGGAGAAGGCCGCCAAGGACGCCTGC
CGGGCGCTGAAAGCCAAGAAGCAGCCCTGCATGGTCCTGCCGCCCAGGGCGTGA

Upstream 100 bases:

>100_bases
TTCAACATCTGTTTACTCTGCATCAAGGCTACGGCTGTCATGGTAGGCAGCTCACGGCGGCGCCGTAGCCGCCCCAACGC
TTTGATTCGACGGACGAAGC

Downstream 100 bases:

>100_bases
GCGGGACCTGAATGGAGAGAGGAAAAGGCCCCAGGCTCGCCCGGGGCCTTTTTCTGTGGGGTCAGCGGCCCAGCAGCCGG
TCCCGGGCGACGTTGAGCTG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6; PBP-6 [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MFQHARRLVLSLGLAAAAAFGAMAPPAQAQIPYQMLAQTSSKYAAIVVDAKTGEVLYENRADSPRYPASITKVMTLYLTF
EALASGKIDLDDRVVFSPRAAAQPPTKLGVRAGDSISVDEAMKALATKSANDAAVALAEKLAGTEQRFAALMTLRARELG
MTDTNFANASGLPDSRQVSTARDIAILSRAVMRDYPQYYKLFSLQYFNFRGQTMRNHNGLLHRMDGVDGLKTGFINASGF
NLAASAVRDGRRLIAVVLGGPTSRARDNNAESLLLTGFEVLDRRARGEDIQVAQSFFESPMTGGRVQPQTAQGDEDSMKI
VLASTAPPIRASKVEIVEKGKWTVQVGSFSSRSDAKEQLSIVEKRFGKHFGKAKGVAEKSGGKYRSRFSGFSEKAAKDAC
RALKAKKQPCMVLPPRA

Sequences:

>Translated_417_residues
MFQHARRLVLSLGLAAAAAFGAMAPPAQAQIPYQMLAQTSSKYAAIVVDAKTGEVLYENRADSPRYPASITKVMTLYLTF
EALASGKIDLDDRVVFSPRAAAQPPTKLGVRAGDSISVDEAMKALATKSANDAAVALAEKLAGTEQRFAALMTLRARELG
MTDTNFANASGLPDSRQVSTARDIAILSRAVMRDYPQYYKLFSLQYFNFRGQTMRNHNGLLHRMDGVDGLKTGFINASGF
NLAASAVRDGRRLIAVVLGGPTSRARDNNAESLLLTGFEVLDRRARGEDIQVAQSFFESPMTGGRVQPQTAQGDEDSMKI
VLASTAPPIRASKVEIVEKGKWTVQVGSFSSRSDAKEQLSIVEKRFGKHFGKAKGVAEKSGGKYRSRFSGFSEKAAKDAC
RALKAKKQPCMVLPPRA
>Mature_417_residues
MFQHARRLVLSLGLAAAAAFGAMAPPAQAQIPYQMLAQTSSKYAAIVVDAKTGEVLYENRADSPRYPASITKVMTLYLTF
EALASGKIDLDDRVVFSPRAAAQPPTKLGVRAGDSISVDEAMKALATKSANDAAVALAEKLAGTEQRFAALMTLRARELG
MTDTNFANASGLPDSRQVSTARDIAILSRAVMRDYPQYYKLFSLQYFNFRGQTMRNHNGLLHRMDGVDGLKTGFINASGF
NLAASAVRDGRRLIAVVLGGPTSRARDNNAESLLLTGFEVLDRRARGEDIQVAQSFFESPMTGGRVQPQTAQGDEDSMKI
VLASTAPPIRASKVEIVEKGKWTVQVGSFSSRSDAKEQLSIVEKRFGKHFGKAKGVAEKSGGKYRSRFSGFSEKAAKDAC
RALKAKKQPCMVLPPRA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=277, Percent_Identity=38.2671480144404, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87082022, Length=302, Percent_Identity=34.7682119205298, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1786851, Length=306, Percent_Identity=33.0065359477124, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082054, Length=226, Percent_Identity=31.4159292035398, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45095; Mature: 45095

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQHARRLVLSLGLAAAAAFGAMAPPAQAQIPYQMLAQTSSKYAAIVVDAKTGEVLYENR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCEEECCC
ADSPRYPASITKVMTLYLTFEALASGKIDLDDRVVFSPRAAAQPPTKLGVRAGDSISVDE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCHHHCCCCCCCCCHHH
AMKALATKSANDAAVALAEKLAGTEQRFAALMTLRARELGMTDTNFANASGLPDSRQVST
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
ARDIAILSRAVMRDYPQYYKLFSLQYFNFRGQTMRNHNGLLHRMDGVDGLKTGFINASGF
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCEEECCCCCCHHHCEECCCCC
NLAASAVRDGRRLIAVVLGGPTSRARDNNAESLLLTGFEVLDRRARGEDIQVAQSFFESP
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCC
MTGGRVQPQTAQGDEDSMKIVLASTAPPIRASKVEIVEKGKWTVQVGSFSSRSDAKEQLS
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEECCCEEEEECCCCCCCHHHHHHH
IVEKRFGKHFGKAKGVAEKSGGKYRSRFSGFSEKAAKDACRALKAKKQPCMVLPPRA
HHHHHHHHHHHHHCCCHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEECCCCC
>Mature Secondary Structure
MFQHARRLVLSLGLAAAAAFGAMAPPAQAQIPYQMLAQTSSKYAAIVVDAKTGEVLYENR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEEEECCCCCEEECCC
ADSPRYPASITKVMTLYLTFEALASGKIDLDDRVVFSPRAAAQPPTKLGVRAGDSISVDE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCHHHCCCCCCCCCHHH
AMKALATKSANDAAVALAEKLAGTEQRFAALMTLRARELGMTDTNFANASGLPDSRQVST
HHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
ARDIAILSRAVMRDYPQYYKLFSLQYFNFRGQTMRNHNGLLHRMDGVDGLKTGFINASGF
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHCCCCCEEECCCCCCHHHCEECCCCC
NLAASAVRDGRRLIAVVLGGPTSRARDNNAESLLLTGFEVLDRRARGEDIQVAQSFFESP
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHCC
MTGGRVQPQTAQGDEDSMKIVLASTAPPIRASKVEIVEKGKWTVQVGSFSSRSDAKEQLS
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHEEECCCEEEEECCCCCCCHHHHHHH
IVEKRFGKHFGKAKGVAEKSGGKYRSRFSGFSEKAAKDACRALKAKKQPCMVLPPRA
HHHHHHHHHHHHHCCCHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3279397; 8905232; 9278503; 7042389 [H]