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The map label for this gene is fadA [H]

Identifier: 197104564

GI number: 197104564

Start: 1247861

End: 1249111

Strand: Reverse

Name: fadA [H]

Synonym: PHZ_c1098

Alternate gene names: 197104564

Gene position: 1249111-1247861 (Counterclockwise)

Preceding gene: 197104571

Following gene: 197104562

Centisome position: 31.26

GC content: 70.66

Gene sequence:

>1251_bases
ATGCCCGAAGCTCTGATCATCGACGCCTGCCGCACGCCGCGCGGCATCGGCAAGGTCGGCAAGGGAGCGCTGGCCGACTT
CCACCCGCAGCACCTGGCCGCCACGGTCCTGAAGGCCCTGGCCGAGCGCAACGGCCTGAAGACCGAGGAAGTCGACGACA
TCGTGTGGGGCACCTCGTCCCAGCGCGGCAAGCAGGGCGGCGACCTCGGCCGGATGGCGGCCCTCGACGCCGGCTACGAC
GTCCGGGCCAGCGGCGTCACCCTCGACCGCTTCTGCGGCTCGGGCATCACCGCCGTGAACATGGCCGCGGCCCAGATCAT
GTCGGGCATGGAGGACCTCGTCATCGCCGGCGGGACCGAGATGATGTCCTACACCGCCGCCACCGCCTCGATGCAGGGTC
CCGACGGCGGCCCGGCTCTGATCGATTCCGGCAACACCCGCCTTCGCGCCCGGCACCCGCAGTCGCACCAGGGCGTCTGC
GCCGACGCCATCGCGACCATGGAAGGGATCACCCGCGAGGCGCTGGACGAGCTCGCCTACGTCAGCCAGCAGCGCGCCGA
CGTGGCCATCCGCGAGGGCCGCTTCGACCGCTCGCTCGTGCCGGTCTATCGCGACGACGGCACGGTGGCCCTCGACCGCG
AGGAGTTCCCCCGCCCGCAGACCACCCGCGAGGGCCTGGCGGGCCTGAAGCCCTCGTTCGAGGCCATGGCCAACGTGCCG
CTGGACGACAAGGGCACGACGCTCGCGGGCCTGATCAACCAGGTCTATCCGGACCTGAAGATCCGCCACTTCCACCACGC
CGGGAACTCCTCGGGCGTGGTGGACGGCGCCGCGGCCGTCCTCCTGGCCTCGCCCGAATACGCCAAGAAGAACGGCCTCA
AGGCCCGCGCCCGCGTGGTCGCCATGGCCAACGTCGGCGATTCCCCGACCCTGATGCTCAACGCCCCGGTCCCGGCGGCG
AAGAAGGTGCTGAAGAAGGCCGGCCTGACCCCCGACGACATCGACCTGTGGGAGATCAACGAGGCGTTCTCGGTCGTCGC
CGAGAAGTTCATCCGCGACCTGAACCTCGACCGCGAGAAGGTCAATGTGAACGGCGGCGCCATGGCCCTCGGGCACCCGA
TCGGCGCCACTGGCTCGATCCTGATCGGCACCGTCATCGACGAGCTGGAACGCCGCGGCCTCCAGCGCGGTCTCGTCACC
ATGTGCGCCGCCGGCGGCATGGCCCCCGCGATCATCGTCGAGCGGGTCTGA

Upstream 100 bases:

>100_bases
CGTACAACATCATGCCGCAGGGGCGAAACGCATTTCCTCTGGACTGACGCCACGTCCGGCAAGCTGATTGGCGCCATCGC
ATTCCCGGGGAGGACGAGAC

Downstream 100 bases:

>100_bases
CCCCGCAAACCTCCCCCGCTCGCGGGGGAGGTTTTCTACTTGGGGCCACCCTTCGTCCACTTCTCCAGCAGCCTGGCCGC
CGCCTCGCGGCCGCCGATGC

Product: acetyl-CoA acetyltransferase

Products: CoA; oxoacyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPEALIIDACRTPRGIGKVGKGALADFHPQHLAATVLKALAERNGLKTEEVDDIVWGTSSQRGKQGGDLGRMAALDAGYD
VRASGVTLDRFCGSGITAVNMAAAQIMSGMEDLVIAGGTEMMSYTAATASMQGPDGGPALIDSGNTRLRARHPQSHQGVC
ADAIATMEGITREALDELAYVSQQRADVAIREGRFDRSLVPVYRDDGTVALDREEFPRPQTTREGLAGLKPSFEAMANVP
LDDKGTTLAGLINQVYPDLKIRHFHHAGNSSGVVDGAAAVLLASPEYAKKNGLKARARVVAMANVGDSPTLMLNAPVPAA
KKVLKKAGLTPDDIDLWEINEAFSVVAEKFIRDLNLDREKVNVNGGAMALGHPIGATGSILIGTVIDELERRGLQRGLVT
MCAAGGMAPAIIVERV

Sequences:

>Translated_416_residues
MPEALIIDACRTPRGIGKVGKGALADFHPQHLAATVLKALAERNGLKTEEVDDIVWGTSSQRGKQGGDLGRMAALDAGYD
VRASGVTLDRFCGSGITAVNMAAAQIMSGMEDLVIAGGTEMMSYTAATASMQGPDGGPALIDSGNTRLRARHPQSHQGVC
ADAIATMEGITREALDELAYVSQQRADVAIREGRFDRSLVPVYRDDGTVALDREEFPRPQTTREGLAGLKPSFEAMANVP
LDDKGTTLAGLINQVYPDLKIRHFHHAGNSSGVVDGAAAVLLASPEYAKKNGLKARARVVAMANVGDSPTLMLNAPVPAA
KKVLKKAGLTPDDIDLWEINEAFSVVAEKFIRDLNLDREKVNVNGGAMALGHPIGATGSILIGTVIDELERRGLQRGLVT
MCAAGGMAPAIIVERV
>Mature_415_residues
PEALIIDACRTPRGIGKVGKGALADFHPQHLAATVLKALAERNGLKTEEVDDIVWGTSSQRGKQGGDLGRMAALDAGYDV
RASGVTLDRFCGSGITAVNMAAAQIMSGMEDLVIAGGTEMMSYTAATASMQGPDGGPALIDSGNTRLRARHPQSHQGVCA
DAIATMEGITREALDELAYVSQQRADVAIREGRFDRSLVPVYRDDGTVALDREEFPRPQTTREGLAGLKPSFEAMANVPL
DDKGTTLAGLINQVYPDLKIRHFHHAGNSSGVVDGAAAVLLASPEYAKKNGLKARARVVAMANVGDSPTLMLNAPVPAAK
KVLKKAGLTPDDIDLWEINEAFSVVAEKFIRDLNLDREKVNVNGGAMALGHPIGATGSILIGTVIDELERRGLQRGLVTM
CAAGGMAPAIIVERV

Specific function: Subunit Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=417, Percent_Identity=35.7314148681055, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI167614485, Length=416, Percent_Identity=33.8942307692308, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI4501853, Length=416, Percent_Identity=35.8173076923077, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI4557237, Length=428, Percent_Identity=32.4766355140187, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI4504327, Length=448, Percent_Identity=30.5803571428571, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=49.438202247191, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI48994986, Length=423, Percent_Identity=37.3522458628842, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082165, Length=421, Percent_Identity=36.8171021377672, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1787663, Length=435, Percent_Identity=39.3103448275862, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788554, Length=424, Percent_Identity=36.5566037735849, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI1788683, Length=453, Percent_Identity=28.2560706401766, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI133906874, Length=400, Percent_Identity=35, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=31.1904761904762, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17535921, Length=422, Percent_Identity=30.8056872037915, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17551802, Length=453, Percent_Identity=28.6975717439294, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535917, Length=424, Percent_Identity=27.5943396226415, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6322031, Length=415, Percent_Identity=33.2530120481928, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6325229, Length=423, Percent_Identity=31.6784869976359, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655093, Length=421, Percent_Identity=34.916864608076, Blast_Score=228, Evalue=5e-60,
Organism=Drosophila melanogaster, GI17648125, Length=420, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24640423, Length=425, Percent_Identity=32.4705882352941, Blast_Score=177, Evalue=9e-45,
Organism=Drosophila melanogaster, GI17137578, Length=459, Percent_Identity=29.6296296296296, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16 [C]

Molecular weight: Translated: 43881; Mature: 43750

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEALIIDACRTPRGIGKVGKGALADFHPQHLAATVLKALAERNGLKTEEVDDIVWGTSS
CCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
QRGKQGGDLGRMAALDAGYDVRASGVTLDRFCGSGITAVNMAAAQIMSGMEDLVIAGGTE
CCCCCCCCHHHHHHCCCCCCEEECCEEHHHHHCCCCHHHHHHHHHHHCCCHHEEEECCHH
MMSYTAATASMQGPDGGPALIDSGNTRLRARHPQSHQGVCADAIATMEGITREALDELAY
HHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VSQQRADVAIREGRFDRSLVPVYRDDGTVALDREEFPRPQTTREGLAGLKPSFEAMANVP
HHHHHHHHHHHCCCCCCCCCCEEECCCCEEECHHHCCCCCCHHHHHHCCCCCHHHHHCCC
LDDKGTTLAGLINQVYPDLKIRHFHHAGNSSGVVDGAAAVLLASPEYAKKNGLKARARVV
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHCCCCHHEEEE
AMANVGDSPTLMLNAPVPAAKKVLKKAGLTPDDIDLWEINEAFSVVAEKFIRDLNLDREK
EEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHE
VNVNGGAMALGHPIGATGSILIGTVIDELERRGLQRGLVTMCAAGGMAPAIIVERV
EECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure 
PEALIIDACRTPRGIGKVGKGALADFHPQHLAATVLKALAERNGLKTEEVDDIVWGTSS
CCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
QRGKQGGDLGRMAALDAGYDVRASGVTLDRFCGSGITAVNMAAAQIMSGMEDLVIAGGTE
CCCCCCCCHHHHHHCCCCCCEEECCEEHHHHHCCCCHHHHHHHHHHHCCCHHEEEECCHH
MMSYTAATASMQGPDGGPALIDSGNTRLRARHPQSHQGVCADAIATMEGITREALDELAY
HHHHHHHHCCCCCCCCCCEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VSQQRADVAIREGRFDRSLVPVYRDDGTVALDREEFPRPQTTREGLAGLKPSFEAMANVP
HHHHHHHHHHHCCCCCCCCCCEEECCCCEEECHHHCCCCCCHHHHHHCCCCCHHHHHCCC
LDDKGTTLAGLINQVYPDLKIRHFHHAGNSSGVVDGAAAVLLASPEYAKKNGLKARARVV
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHCCCCHHEEEE
AMANVGDSPTLMLNAPVPAAKKVLKKAGLTPDDIDLWEINEAFSVVAEKFIRDLNLDREK
EEECCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHE
VNVNGGAMALGHPIGATGSILIGTVIDELERRGLQRGLVTMCAAGGMAPAIIVERV
EECCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acyl-CoA; acetyl-CoA [C]

Specific reaction: Acyl-CoA + acetyl-CoA = CoA + oxoacyl-CoA [C]

General reaction: Acyl group transfer; Claisen condensation [C]

Inhibitor: Tris (hydroxy methyl) aminomethane [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]