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The map label for this gene is gacS [H]

Identifier: 197104507

GI number: 197104507

Start: 1166366

End: 1167031

Strand: Reverse

Name: gacS [H]

Synonym: PHZ_c1041

Alternate gene names: 197104507

Gene position: 1167031-1166366 (Counterclockwise)

Preceding gene: 197104508

Following gene: 197104503

Centisome position: 29.2

GC content: 72.52

Gene sequence:

>666_bases
TTGCAGATTCCGCCGCGCGAAACCCGCCGGCTTAACCGAGGCCGACCTTTCCGCGCGCCGCGTCCTGAACCGCAAAGCTT
GGCCGTAGCCGGCTTCGACTGGCCGGCGCCCCCCCCGACGTCTGGCGTCCGCCCCGCCTTAACGGCGGTTAGCCACGTCC
GTCAGCGTTACCTTAGCTTCTCGGCGCCACATTCCGGACGTATTCGGGCGAGGTCCCGGCCGCGCCCGCCGAAGGAGCTT
GCCGTGAGCGACGCCCAAGGCCCTGCCCGCCGGTTGCGGGTCCTGCACGTGGACGACGACGCCATGAACCTGCGCGTGGT
CCAGGAGATCCTCGAGGCGTTCGGCCACTCGGCCGTGATGGCCGCCTCCGGCGACGAGGCGCTCGAGCACCTCTCGCGCG
AGGCCTTCGACGTGGTGCTGATGGACATCCACATGCCCGGCATGACCGGCGTCGAGGCGGTCCGCCGCCTGCGCGCCTCG
GCCGGGCCCGGCCGCGACACCCCGGTGATCGCCGTCACCGCCGACGTCCTGTCGCGCCGGCCGCAGGAGTACGTGGCCCT
GGGCTTCAACGACTTCGTCGCCAAGCCCATCCTGGTCTCGGGGCTGATGGCCTCGGTCACCCGCGCCGCGGCCGCCCGCG
CCACGCCGCTCTCCCGCGCGAGCTGA

Upstream 100 bases:

>100_bases
GATCATCACGCCCACCAGCCATCCGGCGGTGATGCTCGCTGCGTTCGCGGCCTGCTGCATGCCCGCTAGTTAGCCCGGCC
GCGCGCCCGGGCAAGCACCC

Downstream 100 bases:

>100_bases
ACCCGCGAAGGCCGCTCCTAGTCCGCGTCGGCCTCCACCGGCGGGGCCGCGATCGGCTGCGGCGCGCCCGACAGCGCGTC
CTCCAGCGCCTGCCGGTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MQIPPRETRRLNRGRPFRAPRPEPQSLAVAGFDWPAPPPTSGVRPALTAVSHVRQRYLSFSAPHSGRIRARSRPRPPKEL
AVSDAQGPARRLRVLHVDDDAMNLRVVQEILEAFGHSAVMAASGDEALEHLSREAFDVVLMDIHMPGMTGVEAVRRLRAS
AGPGRDTPVIAVTADVLSRRPQEYVALGFNDFVAKPILVSGLMASVTRAAAARATPLSRAS

Sequences:

>Translated_221_residues
MQIPPRETRRLNRGRPFRAPRPEPQSLAVAGFDWPAPPPTSGVRPALTAVSHVRQRYLSFSAPHSGRIRARSRPRPPKEL
AVSDAQGPARRLRVLHVDDDAMNLRVVQEILEAFGHSAVMAASGDEALEHLSREAFDVVLMDIHMPGMTGVEAVRRLRAS
AGPGRDTPVIAVTADVLSRRPQEYVALGFNDFVAKPILVSGLMASVTRAAAARATPLSRAS
>Mature_221_residues
MQIPPRETRRLNRGRPFRAPRPEPQSLAVAGFDWPAPPPTSGVRPALTAVSHVRQRYLSFSAPHSGRIRARSRPRPPKEL
AVSDAQGPARRLRVLHVDDDAMNLRVVQEILEAFGHSAVMAASGDEALEHLSREAFDVVLMDIHMPGMTGVEAVRRLRAS
AGPGRDTPVIAVTADVLSRRPQEYVALGFNDFVAKPILVSGLMASVTRAAAARATPLSRAS

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI48994928, Length=121, Percent_Identity=36.3636363636364, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789149, Length=153, Percent_Identity=32.0261437908497, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI145693157, Length=135, Percent_Identity=31.8518518518519, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=121, Percent_Identity=30.5785123966942, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322000, Length=179, Percent_Identity=26.8156424581006, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 23996; Mature: 23996

Theoretical pI: Translated: 11.84; Mature: 11.84

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPPRETRRLNRGRPFRAPRPEPQSLAVAGFDWPAPPPTSGVRPALTAVSHVRQRYLSF
CCCCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
SAPHSGRIRARSRPRPPKELAVSDAQGPARRLRVLHVDDDAMNLRVVQEILEAFGHSAVM
CCCCCCCEECCCCCCCHHHHHCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHCCCEEE
AASGDEALEHLSREAFDVVLMDIHMPGMTGVEAVRRLRASAGPGRDTPVIAVTADVLSRR
ECCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCC
PQEYVALGFNDFVAKPILVSGLMASVTRAAAARATPLSRAS
CHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQIPPRETRRLNRGRPFRAPRPEPQSLAVAGFDWPAPPPTSGVRPALTAVSHVRQRYLSF
CCCCCHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
SAPHSGRIRARSRPRPPKELAVSDAQGPARRLRVLHVDDDAMNLRVVQEILEAFGHSAVM
CCCCCCCEECCCCCCCHHHHHCCCCCCHHHEEEEEEECCCHHHHHHHHHHHHHHCCCEEE
AASGDEALEHLSREAFDVVLMDIHMPGMTGVEAVRRLRASAGPGRDTPVIAVTADVLSRR
ECCCHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCC
PQEYVALGFNDFVAKPILVSGLMASVTRAAAARATPLSRAS
CHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]