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The map label for this gene is hsdM [H]

Identifier: 197104449

GI number: 197104449

Start: 1087216

End: 1088673

Strand: Reverse

Name: hsdM [H]

Synonym: PHZ_c0983

Alternate gene names: 197104449

Gene position: 1088673-1087216 (Counterclockwise)

Preceding gene: 197104450

Following gene: 197104448

Centisome position: 27.24

GC content: 64.75

Gene sequence:

>1458_bases
ATGAGCGTTCGCACTATCGTCAAATCCATCCAGGACGAGATGCGCAAGGATGCCGGCGTCGACGGCGATGCCCAGCGCAT
CAGCCAGCTCTGCTGGATGTTCTTCCTCAAGATCATCGACGACCAGGACCAGGAGCTGGAGTTGGTCCAGCCGGGATACG
AGTCGCCTGTGCCGGACGAGTTCCAGTGGCGAAACTGGGCCGCTGATCGGGAAGGCATGACCGGCGACGCGCTGCTGAGC
TTCATCAACAACGAGCTGTTTCCAGCCCTCAAGAACCTGCCCATCACCGGTCCGCGGCGCCACCGCGCCATCGTGGTCCG
CAGCGTCTTCGAGGACGCCTACAACTACATGAAGTCCGGCCACCAGCTGCGGAAGGTGGTCAACAAGATCGACGATGTCG
ACTTCAATGACCTGTCCGAGCGCCAGCACTTTGGAGACATCTACGAGCAGCTTCTCAACGACCTGCAGAGCGCTGGCAAC
GCAGGCGAGTACTACACGCCCCGCGCCGTCACGGCGTTCATGACGGACCGGATCGACCCGAAGCCAGGCGAGATCCTCTT
CGACCCAGCCTGCGGCACCGGCGGCTTCCTCACCTGCGCGATGCGCCACATGCGCGAGCGCTATGTGAAGCGGCCGGAAG
ACGAGGCCTTAATGCAGGCCTCGCTTCGCGCGGTGGAGAAAAAGCCGCTACCGCACATGCTGGCGGTCACCAACATGCTG
CTGCATGGGGTCGAGGATCCCAGCTTCCTGCGCCACGACAACACCCTCGCGCGGCCCTACATCAGCTGGGGTCAGTCCGA
CCGCGTCGACATCGTCCTTACCAACCCGCCGTTCGGCGGCCAGGAAGAGGACGGCATCGAGACCAACTTCCCGGCCCACT
TCCGCACGCGCGAGACGGCCGACCTCTTCCTGGCGCTAATCGTCCGTCTCCTGAAGCCGGGCGGCCGCGCGGCGGTGGTG
CTGCCCGACGGCACACTCTTCGGCGAGGGCATGAAGACCCGGCTCAAGGAGCACCTGATGGAGGAGTGCAACCTCCACAC
CATCGTCCGGCTCCCCAACTCGGTCTTCAAGCCCTACGCCTCCATCGGCACCAACCTCTTGTTCTTCGAGAAGGGGCAGC
CGACGGAGGAGACTTGGTATTGGGAGCATCGGGTCCCCGAGGGCCAGAAGGCCTATTCCATGACCAAGCCCATCCGGCTT
GAGCACTTCGCCGACTGCGTCGCCTGGTGGGGCGGCACAGCGCGTGAGGCACGGCAGGAAGGGCCGCAGGCTTGGCGCGT
GACCGCCGAGGCGGTGAAGGCGCGCAACTACAACCTCGACATCAAGAACCCGCACACCGTCGAGGAGGACCATGGCGACC
CCGCTGAGCTGTTGGCCGAGCTGCGCGCGGCAGAAGCGAAGGCTGCTGGCCTGCGCGACCAGCTGAAGTCGATCCTGGCC
GAGGCGCTCGCGCGATGA

Upstream 100 bases:

>100_bases
TTGCCGGCGTGGGCGTGCTGTGTCCCATCCGCCCGAACGGCCAGTGGTACGTCGCCGACATCGAGGCTGCACTGTACGCT
GACATTATAGGTCTTGAGTC

Downstream 100 bases:

>100_bases
ACGCGGAGCGGCTGTTGGCGCACTACGAGGAGATCGCCGAGGCACCAGATGCGGTGGCGAGGCTACGGCGGTTCGTAGTG
GATATCGCGGTACGAGGCGC

Product: type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSVRTIVKSIQDEMRKDAGVDGDAQRISQLCWMFFLKIIDDQDQELELVQPGYESPVPDEFQWRNWAADREGMTGDALLS
FINNELFPALKNLPITGPRRHRAIVVRSVFEDAYNYMKSGHQLRKVVNKIDDVDFNDLSERQHFGDIYEQLLNDLQSAGN
AGEYYTPRAVTAFMTDRIDPKPGEILFDPACGTGGFLTCAMRHMRERYVKRPEDEALMQASLRAVEKKPLPHMLAVTNML
LHGVEDPSFLRHDNTLARPYISWGQSDRVDIVLTNPPFGGQEEDGIETNFPAHFRTRETADLFLALIVRLLKPGGRAAVV
LPDGTLFGEGMKTRLKEHLMEECNLHTIVRLPNSVFKPYASIGTNLLFFEKGQPTEETWYWEHRVPEGQKAYSMTKPIRL
EHFADCVAWWGGTAREARQEGPQAWRVTAEAVKARNYNLDIKNPHTVEEDHGDPAELLAELRAAEAKAAGLRDQLKSILA
EALAR

Sequences:

>Translated_485_residues
MSVRTIVKSIQDEMRKDAGVDGDAQRISQLCWMFFLKIIDDQDQELELVQPGYESPVPDEFQWRNWAADREGMTGDALLS
FINNELFPALKNLPITGPRRHRAIVVRSVFEDAYNYMKSGHQLRKVVNKIDDVDFNDLSERQHFGDIYEQLLNDLQSAGN
AGEYYTPRAVTAFMTDRIDPKPGEILFDPACGTGGFLTCAMRHMRERYVKRPEDEALMQASLRAVEKKPLPHMLAVTNML
LHGVEDPSFLRHDNTLARPYISWGQSDRVDIVLTNPPFGGQEEDGIETNFPAHFRTRETADLFLALIVRLLKPGGRAAVV
LPDGTLFGEGMKTRLKEHLMEECNLHTIVRLPNSVFKPYASIGTNLLFFEKGQPTEETWYWEHRVPEGQKAYSMTKPIRL
EHFADCVAWWGGTAREARQEGPQAWRVTAEAVKARNYNLDIKNPHTVEEDHGDPAELLAELRAAEAKAAGLRDQLKSILA
EALAR
>Mature_484_residues
SVRTIVKSIQDEMRKDAGVDGDAQRISQLCWMFFLKIIDDQDQELELVQPGYESPVPDEFQWRNWAADREGMTGDALLSF
INNELFPALKNLPITGPRRHRAIVVRSVFEDAYNYMKSGHQLRKVVNKIDDVDFNDLSERQHFGDIYEQLLNDLQSAGNA
GEYYTPRAVTAFMTDRIDPKPGEILFDPACGTGGFLTCAMRHMRERYVKRPEDEALMQASLRAVEKKPLPHMLAVTNMLL
HGVEDPSFLRHDNTLARPYISWGQSDRVDIVLTNPPFGGQEEDGIETNFPAHFRTRETADLFLALIVRLLKPGGRAAVVL
PDGTLFGEGMKTRLKEHLMEECNLHTIVRLPNSVFKPYASIGTNLLFFEKGQPTEETWYWEHRVPEGQKAYSMTKPIRLE
HFADCVAWWGGTAREARQEGPQAWRVTAEAVKARNYNLDIKNPHTVEEDHGDPAELLAELRAAEAKAAGLRDQLKSILAE
ALAR

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=413, Percent_Identity=29.2978208232446, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55073; Mature: 54942

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCC
>Mature Secondary Structure 
SVRTIVKSIQDEMRKDAGVDGDAQRISQLCWMFFLKIIDDQDQELELVQPGYESPVPDE
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCCC
FQWRNWAADREGMTGDALLSFINNELFPALKNLPITGPRRHRAIVVRSVFEDAYNYMKSG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
HQLRKVVNKIDDVDFNDLSERQHFGDIYEQLLNDLQSAGNAGEYYTPRAVTAFMTDRIDP
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
KPGEILFDPACGTGGFLTCAMRHMRERYVKRPEDEALMQASLRAVEKKPLPHMLAVTNML
CCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHH
LHGVEDPSFLRHDNTLARPYISWGQSDRVDIVLTNPPFGGQEEDGIETNFPAHFRTRETA
HHCCCCCHHHHCCCCHHCHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
DLFLALIVRLLKPGGRAAVVLPDGTLFGEGMKTRLKEHLMEECNLHTIVRLPNSVFKPYA
HHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
SIGTNLLFFEKGQPTEETWYWEHRVPEGQKAYSMTKPIRLEHFADCVAWWGGTAREARQE
HHCCCEEEEECCCCCCCCEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHC
GPQAWRVTAEAVKARNYNLDIKNPHTVEEDHGDPAELLAELRAAEAKAAGLRDQLKSILA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALAR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]