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The map label for this gene is flbD [H]

Identifier: 197104324

GI number: 197104324

Start: 950739

End: 952106

Strand: Reverse

Name: flbD [H]

Synonym: PHZ_c0858

Alternate gene names: 197104324

Gene position: 952106-950739 (Counterclockwise)

Preceding gene: 197104325

Following gene: 197104323

Centisome position: 23.82

GC content: 71.27

Gene sequence:

>1368_bases
ATGCGGCTTCTGGTCGTCGGAAAACTGAGCGGACAGCTCTCCGCCGCCGTGAAGATGGCGATGAGCGCGGGCGCCAAGGT
CAGCCACGTGGAGACGATCCTCGCGGCGACGCACGCCCTGCGCGCCGGCCAGGGCGCCGACCTCCTGATGGTCGACTACG
AGCTCGACATCGCGGGCCTGATCGAGGCCAACGAGGCCGAGCGGATCCGGGTGCCGGTGGTGGCCTGCGGCGTGGGGGCC
GACCCCAAGCGCGCGGCCGACGCGATCCGCGCCGGGGCCAAGGAGTTCATCCCCCTGCCGCCCGAGGCCGAGCTGATCGC
CGCGGTGCTGGCCGCGGTCAGCGACGACAACCGTCCGATGGTGGTGCGCGACCCGGCCATGGAGCAGGTCATCCAGCTGG
CCGACCAGGTGGCGCCGTCCGACGCCTCGATCCTGATCACCGGGGAAAGCGGCTCGGGCAAGGAAGTCATCGCCCGCTAC
GTCCACCAGAAGTCCCGCCGCGCGAGCCGGCCGTTCATCTCGGTGAACTGCGCGGCCATCCCCGAGAACCTCCTGGAGAG
CGAACTCTTCGGCCACGAGAAGGGCGCCTTCACCGGCGCGGTGGCCCGCCGGATCGGCAAGTTCGAGGAGGCCAACGGCG
GCACCCTGCTGCTGGACGAAATCTCGGAGATGGACGCCCGGCTGCAGGCCAAGCTGCTGCGGGCGCTGCAGGAACGCGAG
ATCGACCGCGTCGGCGGGACCAAGCCGGTGAAGGTGGACATCCGCATCCTGGCCACCTCGAACCGCGACCTGGCCCAGGC
GGTGAAGGAAGGGACGTTCCGCGAGGACCTGCTCTACCGCCTGAACGTCGTGAACCTGCGCCTGCCGCCGCTGCGGGAGC
GGCCGGGCGACGTCGTCGCCCTGGCCGAGCACTTCGTGAAGAAGTACGCCGCCGCCAACGGCATGCCCGAGAAGCCGCTG
TCGGCCGAGGCCAAGCGGCGCCTGGCCGCCCACCGCTGGCCCGGCAACGTGCGTGAGCTCGAGAACGCCATGCACCGCGC
GGTGCTCCTGGCGACCGGGACCGAGATCGACGAAATGGCCATCCGCCTGCCCGACGGCCAGCCGCTGGCCCCGGCCGATC
CGCACGCCAGGGCCGCCCAGGCCGCCTCTCTGGCGGCCGAGACCGCCAGCCGCTCATTCGTCGGCCAGACCGTGGCCGAG
ATGGAGCAGCAGCTGATCCTCGACACGCTGGAGCACTGCCTGGGCAACCGCACGCACGCGGCCAACATCCTCGGCATCTC
GATCCGCACGCTGCGTAACAAGCTGAAGGAATACGCCGAGGCCGGCGTGTCGGTGCCGGCGCCGCAGATGGGCGCGAGCG
CGGCCTGA

Upstream 100 bases:

>100_bases
GTCGTCATCGTCGACGAGCGCCTGGGCGTGACCATGACGGAAATCATCAAGGACGGTGACGCCGCGGCCTGAGGCCGGGG
CGGGGTGAAGGAGATTGCCG

Downstream 100 bases:

>100_bases
CGCGCGGGGCTTTGAGGGGGGCCTATGTCTGACACCGGTACGGCCGGGCGCCTTTCGGGCGCGCCGTCGCCGAAGGAAAT
CTGGGGCTGGTTCGCCCGGG

Product: transcriptional regulator FlbD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MRLLVVGKLSGQLSAAVKMAMSAGAKVSHVETILAATHALRAGQGADLLMVDYELDIAGLIEANEAERIRVPVVACGVGA
DPKRAADAIRAGAKEFIPLPPEAELIAAVLAAVSDDNRPMVVRDPAMEQVIQLADQVAPSDASILITGESGSGKEVIARY
VHQKSRRASRPFISVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDARLQAKLLRALQERE
IDRVGGTKPVKVDIRILATSNRDLAQAVKEGTFREDLLYRLNVVNLRLPPLRERPGDVVALAEHFVKKYAAANGMPEKPL
SAEAKRRLAAHRWPGNVRELENAMHRAVLLATGTEIDEMAIRLPDGQPLAPADPHARAAQAASLAAETASRSFVGQTVAE
MEQQLILDTLEHCLGNRTHAANILGISIRTLRNKLKEYAEAGVSVPAPQMGASAA

Sequences:

>Translated_455_residues
MRLLVVGKLSGQLSAAVKMAMSAGAKVSHVETILAATHALRAGQGADLLMVDYELDIAGLIEANEAERIRVPVVACGVGA
DPKRAADAIRAGAKEFIPLPPEAELIAAVLAAVSDDNRPMVVRDPAMEQVIQLADQVAPSDASILITGESGSGKEVIARY
VHQKSRRASRPFISVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDARLQAKLLRALQERE
IDRVGGTKPVKVDIRILATSNRDLAQAVKEGTFREDLLYRLNVVNLRLPPLRERPGDVVALAEHFVKKYAAANGMPEKPL
SAEAKRRLAAHRWPGNVRELENAMHRAVLLATGTEIDEMAIRLPDGQPLAPADPHARAAQAASLAAETASRSFVGQTVAE
MEQQLILDTLEHCLGNRTHAANILGISIRTLRNKLKEYAEAGVSVPAPQMGASAA
>Mature_455_residues
MRLLVVGKLSGQLSAAVKMAMSAGAKVSHVETILAATHALRAGQGADLLMVDYELDIAGLIEANEAERIRVPVVACGVGA
DPKRAADAIRAGAKEFIPLPPEAELIAAVLAAVSDDNRPMVVRDPAMEQVIQLADQVAPSDASILITGESGSGKEVIARY
VHQKSRRASRPFISVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDARLQAKLLRALQERE
IDRVGGTKPVKVDIRILATSNRDLAQAVKEGTFREDLLYRLNVVNLRLPPLRERPGDVVALAEHFVKKYAAANGMPEKPL
SAEAKRRLAAHRWPGNVRELENAMHRAVLLATGTEIDEMAIRLPDGQPLAPADPHARAAQAASLAAETASRSFVGQTVAE
MEQQLILDTLEHCLGNRTHAANILGISIRTLRNKLKEYAEAGVSVPAPQMGASAA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=414, Percent_Identity=43.9613526570048, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1790437, Length=409, Percent_Identity=40.5867970660147, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI87082117, Length=330, Percent_Identity=45.4545454545455, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1790299, Length=424, Percent_Identity=38.9150943396226, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=44.1640378548896, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789087, Length=320, Percent_Identity=44.6875, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788905, Length=408, Percent_Identity=37.0098039215686, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=51.4893617021277, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=39.8753894080997, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=40.251572327044, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=41.6167664670659, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1789828, Length=247, Percent_Identity=38.0566801619433, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI87081858, Length=341, Percent_Identity=28.4457478005865, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48721; Mature: 48721

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLVVGKLSGQLSAAVKMAMSAGAKVSHVETILAATHALRAGQGADLLMVDYELDIAGL
CEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEE
IEANEAERIRVPVVACGVGADPKRAADAIRAGAKEFIPLPPEAELIAAVLAAVSDDNRPM
EECCCCCEEEEEEEEECCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCCE
VVRDPAMEQVIQLADQVAPSDASILITGESGSGKEVIARYVHQKSRRASRPFISVNCAAI
EEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHC
PENLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDARLQAKLLRALQERE
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
IDRVGGTKPVKVDIRILATSNRDLAQAVKEGTFREDLLYRLNVVNLRLPPLRERPGDVVA
HHHCCCCCCEEEEEEEEEECCHHHHHHHHHCCHHHHHHHHHEEEEEECCCCCCCCCCCHH
LAEHFVKKYAAANGMPEKPLSAEAKRRLAAHRWPGNVRELENAMHRAVLLATGTEIDEMA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCHHHHE
IRLPDGQPLAPADPHARAAQAASLAAETASRSFVGQTVAEMEQQLILDTLEHCLGNRTHA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ANILGISIRTLRNKLKEYAEAGVSVPAPQMGASAA
CCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLLVVGKLSGQLSAAVKMAMSAGAKVSHVETILAATHALRAGQGADLLMVDYELDIAGL
CEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEEE
IEANEAERIRVPVVACGVGADPKRAADAIRAGAKEFIPLPPEAELIAAVLAAVSDDNRPM
EECCCCCEEEEEEEEECCCCCHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHCCCCCCE
VVRDPAMEQVIQLADQVAPSDASILITGESGSGKEVIARYVHQKSRRASRPFISVNCAAI
EEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHC
PENLLESELFGHEKGAFTGAVARRIGKFEEANGGTLLLDEISEMDARLQAKLLRALQERE
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHH
IDRVGGTKPVKVDIRILATSNRDLAQAVKEGTFREDLLYRLNVVNLRLPPLRERPGDVVA
HHHCCCCCCEEEEEEEEEECCHHHHHHHHHCCHHHHHHHHHEEEEEECCCCCCCCCCCHH
LAEHFVKKYAAANGMPEKPLSAEAKRRLAAHRWPGNVRELENAMHRAVLLATGTEIDEMA
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHEEEEECCCCHHHHE
IRLPDGQPLAPADPHARAAQAASLAAETASRSFVGQTVAEMEQQLILDTLEHCLGNRTHA
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ANILGISIRTLRNKLKEYAEAGVSVPAPQMGASAA
CCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]